Evaluation of matrix metalloproteinase-2 (mmp-2) activity with film in situ zymography for improved cytological diagnosis of Breast tumors

Breast Cancer ◽  
2006 ◽  
Vol 13 (3) ◽  
pp. 272-278 ◽  
Author(s):  
Akira Fujiwara ◽  
Eri Shibata ◽  
Hitomi Terashima ◽  
Akemi Shishido ◽  
Junko Nishiki ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Breast Tumors ◽  
Matrix Metalloproteinase 2 ◽  
Cytological Diagnosis ◽  
Film In Situ Zymography ◽  
In Situ Zymography

High resolution in situ zymography reveals matrix metalloproteinase activity at glutamatergic synapses

Neuroscience ◽  
2009 ◽  
Vol 158 (1) ◽  
pp. 167-176 ◽  
Author(s):  
M. Gawlak ◽  
T. Górkiewicz ◽  
A. Gorlewicz ◽  
F.A. Konopacki ◽  
L. Kaczmarek ◽  
...  
Keyword(s):  
High Resolution ◽  
Matrix Metalloproteinase ◽  
Glutamatergic Synapses ◽  
In Situ Zymography ◽  
Metalloproteinase Activity

Programmed microcapsule-type matrix metalloproteinase-2 (MMP-2)-responsive nanosensor for in situ monitoring of intracellular MMP-2

2018 ◽  
Vol 273 ◽  
pp. 511-518 ◽  
Author(s):  
XiaoJing Zhang ◽  
ZhaoYi Miao ◽  
Yue Hu ◽  
XiaoTong Yang ◽  
YingYing Tang ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
In Situ Monitoring ◽  
Matrix Metalloproteinase 2

scholarly journals Association of Matrix Metalloproteinase-2 mRNA Expression with Subtypes of Pediatric Cholesteatoma

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Taichi Kan ◽  
Hiromi Ueda ◽  
Taishi Takahara ◽  
Yoshimasa Tsuchiya ◽  
Mayuko Kishimoto ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Matrix Metalloproteinase ◽  
Mrna Expression ◽  
Bone Destruction ◽  
Expression Level ◽  
Matrix Metalloproteinase 2 ◽  
Mrna Expression Level ◽  
Congenital Cholesteatoma ◽  
Rna In Situ Hybridization

Objective. Cholesteatoma is a clinically heterogeneous disease, with some patients showing spontaneous regression, while others experiencing an aggressive, lethal disease. Cholesteatoma in children can be divided into two types: congenital and acquired. Identifying good prognostic markers is needed to help select patients who will require immediate surgical intervention. Matrix metalloproteinase-2 (MMP2) was previously reported to play an important role in cholesteatoma progression, by promoting bone destruction and keratinocyte infiltration. Herein, we analyzed MMP2 mRNA expression level in cholesteatoma using RNA-in situ hybridization in formalin-fixed, paraffin-embedded (FFPE) tissue samples. Methods. Sixty patients with cholesteatoma under 15 years old, who underwent their primary surgery at Aichi Medical University’s Otolaryngology Department, were analyzed for MMP2 expression level, using RNA-in situ hybridization. Results. There were no significant differences in MMP2 mRNA expression level between congenital cholesteatoma and acquired cholesteatomas. In congenital cholesteatoma, higher MMP2 signals were observed in the open type than in the closed type ( p < 0.001 ). In acquired cholesteatoma, higher MMP2 signals were observed in the pars tensa than in the pars flaccida ( p < 0.001 ). MMP2 mRNA expression level was almost exclusively found in the fibroblasts or in the inflammatory cells in the stroma, but not in the epithelium. Conclusion. Our study reveals that MMP2 mRNA expression level is strongly associated with the subtypes of cholesteatoma. The findings suggest that the level of expression of MMP2 mRNA may be related to the pathogenesis and aggressive features of cholesteatoma.

scholarly journals Effect of epithelial growth factor on matrix metalloproteinase-2 and E-cadherin/β-catenin expression in an in situ model of tumorigenesis

2017 ◽  
Vol 14 (3) ◽  
pp. 3136-3140
Author(s):  
Natalia Festugatto Navarini ◽  
Vera Cavalcanti De Araújo ◽  
Marcelo Sperandio ◽  
Marcelo Henrique Napimoga ◽  
Lucas Novaes Teixeira ◽  
...  
Keyword(s):  
Growth Factor ◽  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 2 ◽  
Epithelial Growth Factor ◽  
Epithelial Growth ◽  
E Cadherin
Sign in / Sign up

Export Citation Format

Share Document