scholarly journals Detections of matrix metalloproteinases activities and localization by Film in situ zymography (FIZ).

2003 ◽  
Vol 121 (2) ◽  
pp. 113-118 ◽  
Author(s):  
Michiko MURAMATSU ◽  
Shinji TAKAI ◽  
Mizuo MIYAZAKI
Keyword(s):  
Matrix Metalloproteinases ◽  
Film In Situ Zymography ◽  
In Situ Zymography

Evaluation of matrix metalloproteinase-2 (mmp-2) activity with film in situ zymography for improved cytological diagnosis of Breast tumors

Breast Cancer ◽  
2006 ◽  
Vol 13 (3) ◽  
pp. 272-278 ◽  
Author(s):  
Akira Fujiwara ◽  
Eri Shibata ◽  
Hitomi Terashima ◽  
Akemi Shishido ◽  
Junko Nishiki ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Breast Tumors ◽  
Matrix Metalloproteinase 2 ◽  
Cytological Diagnosis ◽  
Film In Situ Zymography ◽  
In Situ Zymography

Localization of gelatinase activities in glioma tissues by film in situ zymography

2001 ◽  
Vol 18 (2) ◽  
pp. 145-150 ◽  
Author(s):  
Shingo Takano ◽  
Koji Tsuboi ◽  
Akira Matsumura ◽  
Hiroshige Sato ◽  
Tadao Nose
Keyword(s):  
Film In Situ Zymography ◽  
In Situ Zymography

scholarly journals Inward remodeling of resistance arteries requires reactive oxygen species-dependent activation of matrix metalloproteinases

2011 ◽  
Vol 300 (6) ◽  
pp. H2005-H2015 ◽  
Author(s):  
Luis A. Martinez-Lemus ◽  
Guiling Zhao ◽  
Edgar L. Galiñanes ◽  
Matthew Boone
Keyword(s):  
Matrix Metalloproteinases ◽  
Prolonged Exposure ◽  
Multiphoton Microscopy ◽  
Ros Production ◽  
Ang Ii ◽  
Resistance Arteries ◽  
Resistance Vessels ◽  
In Situ Zymography ◽  
Eutrophic Remodeling

Inward eutrophic remodeling is the most prevalent structural change of resistance arteries in hypertension. Sympathetic and angiotensin (ANG)-induced vasoconstriction has been associated with hypertension and with the production of matrix metalloproteinases (MMPs) and ROS. Therefore, we hypothesize that prolonged exposure to norepinephrine (NE) and ANG II induces arteriolar inward remodeling dependent on the activation of MMPs and the production of ROS. This hypothesis was tested on rat cremaster arterioles that were isolated, cannulated, pressurized, and exposed to either NE (10−5.5 mol/l) + ANG II (10−7 mol/l) or vehicle (control) for 4 h. The prolonged exposure to NE + ANG II induced inward remodeling, as evidenced by the reduced maximal arteriolar passive diameter observed after versus before exposure to the vasoconstrictor agonists. NE + ANG II also increased the arteriolar expression and activity of MMP-2 and the production of ROS as determined, respectively, by real-time RT-PCR, gel and in situ zymography, and the use of ROS-sensitive dyes with multiphoton microscopy. Inhibition of MMP activation (with GM-6001) or ROS production (with apocynin or tempol) prevented the NE + ANG II-induced inward remodeling. Inhibition of ROS production prevented the activation of MMPs and the remodeling process, whereas inhibition of MMP activation did not affect ROS production. These results indicate that prolonged stimulation of resistance arterioles with NE + ANG II induces a ROS-dependent activation of MMPs necessary for the development of arteriolar inward remodeling. These mechanisms may contribute to the structural narrowing of resistance vessels in hypertension.

Matrix Metalloproteinases-1, -3, and -8 in Adult Periodontitis in Situ.

1994 ◽  
Vol 732 (1 Inhibition of) ◽  
pp. 459-461 ◽  
Author(s):  
T. INGMAN ◽  
T. SORSA ◽  
J. MICHAELIS ◽  
Y. T. KONTTINEN
Keyword(s):  
Matrix Metalloproteinases

In Vivo Localization of Gelatinases (MMP-2 and -9) by in Situ Zymography with a Selective Gelatinase Inhibitor

2001 ◽  
Vol 287 (3) ◽  
pp. 766-774 ◽  
Author(s):  
Emma Pirilä ◽  
Päivi Maisi ◽  
Tuula Salo ◽  
Erkki Koivunen ◽  
Timo Sorsa
Keyword(s):  
In Situ Zymography
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