The Barley Stripe Mosaic Virus gb Gene Encodes a Multifunctional Cysteine-Rich Protein That Affects Pathogenesis

1994 ◽  
Vol 6 (11) ◽  
pp. 1593 ◽  
Author(s):  
Robert G. K. Donald ◽  
Andrew O. Jackson
Keyword(s):  
Mosaic Virus ◽  
Barley Stripe Mosaic Virus ◽  
Cysteine Rich Protein

scholarly journals The N-Terminal 85 Amino Acids of the Barley Stripe Mosaic Virus γb Pathogenesis Protein Contain Three Zinc-Binding Motifs

2004 ◽  
Vol 78 (14) ◽  
pp. 7379-7391 ◽  
Author(s):  
Jennifer N. Bragg ◽  
Diane M. Lawrence ◽  
Andrew O. Jackson
Keyword(s):  
Mosaic Virus ◽  
Binding Activity ◽  
Zinc Binding ◽  
Barley Stripe Mosaic Virus ◽  
Deletion Mutants ◽  
Chenopodium Amaranticolor ◽  
Binding Motifs ◽  
Cysteine Rich Protein ◽  
Local Lesions ◽  
Arginine Residues

ABSTRACT Barley stripe mosaic virus RNAγ encodes γb, a cysteine-rich protein that affects pathogenesis. Nine of the eleven cysteines are concentrated in two clusters, designated C1 (residues 1 to 23) and C2 (residues 60 to 85), that are arranged in zinc finger-like motifs. A basic motif (BM) rich in lysine and arginine (residues 19 to 47) resides between the C1 and C2 clusters. We have demonstrated that γb binds zinc and that the C1, BM, and C2 motifs have independent zinc-binding activities. To evaluate the requirements for binding, mutations were introduced into each region. Cysteine residues at positions 7, 9, 10, 19, and 23 in the C1 motif were replaced with serines. In the BM, asparagines were substituted for lysines at positions 26 and 35, glutamine for arginine at position 25, and glycines for arginines at positions 33 and 36. The C2 mutations included cysteine replacements with serines at positions 60, 64, 71, and 81, and a histidine-to-leucine change at position 85. These mutations destroyed zinc-binding activity in each of the isolated motifs. γb derivatives containing mutations in only two of the motifs retained the ability to bind zinc, whereas a γb derivative containing mutations inactivating all three motifs destroyed the ability to bind zinc. Plants inoculated with transcripts containing combinations of the C1, BM, and C2 mutations elicited a “null” phenotype in barley characteristic of γb deletion mutants and also delayed the appearance and reduced the size of local lesions in Chenopodium amaranticolor. These results show that zinc binding of each of the motifs is critical for the biological activity of γb.

Comparisons of poa semilatent and barley stripe mosaic viruses

1972 ◽  
Vol 50 (2) ◽  
pp. 263-267 ◽  
Author(s):  
Z. Polák ◽  
J. T. Slykhuis
Keyword(s):  
Mosaic Virus ◽  
Double Diffusion ◽  
Barley Stripe Mosaic Virus ◽  
Diffusion Test ◽  
Partial Protection ◽  
Normal Length ◽  
Local Lesions ◽  
Cross Absorption ◽  
Precipitin Test ◽  
Agropyron Trachycaulum

Poa semilatent virus (PSLV), which caused chlorosis and rapid death of inoculated wheat, infected a number of grasses susceptible to barley stripe mosaic virus (BSMV); but it also infected Agropyron trachycaulum and Poa palustris which were not susceptible to a barley strain or two oat-infecting strains of BSMV. Conversely BSMV caused local lesions on several species of Chenopodium that did not become infected with PSLV. BSMV protected wheat from infection by PSLV, but PSLV caused only partial protection from BSMV.The normal length and thickness of particles in leaf-dip preparations was 161 × 26 mμ for PSLV and 133 × 25 mμ for BSMV.Serological relationship was not indicated by the Ouchterlony agar double-diffusion test or leaf-dip serology. A distant relationship was shown with the microprecipitin test and the ring interface precipitin test. In cross absorption tests the titers of the antisera to the homologous viruses were not reduced by absorption with the heterologous viruses.PSLV and BSMV appear to be distantly related serotypes.

scholarly journals Barley Stripe Mosaic Virus (BSMV) Induced MicroRNA Silencing in Common Wheat (Triticum aestivum L.)

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0126621 ◽  
Author(s):  
Jian Jiao ◽  
Yichun Wang ◽  
Jonathan Nimal Selvaraj ◽  
Fuguo Xing ◽  
Yang Liu
Keyword(s):  
Triticum Aestivum ◽  
Mosaic Virus ◽  
Common Wheat ◽  
Triticum Aestivum L ◽  
Barley Stripe Mosaic Virus

scholarly journals Barley Stripe Mosaic Virus γb Interacts with Glycolate Oxidase and Inhibits Peroxisomal ROS Production to Facilitate Virus Infection

2018 ◽  
Vol 11 (2) ◽  
pp. 338-341 ◽  
Author(s):  
Meng Yang ◽  
Zhenggang Li ◽  
Kun Zhang ◽  
Xuan Zhang ◽  
Yongliang Zhang ◽  
...  
Keyword(s):  
Virus Infection ◽  
Mosaic Virus ◽  
Glycolate Oxidase ◽  
Barley Stripe Mosaic Virus ◽  
Ros Production

scholarly journals Subcellular Localization of the Barley Stripe Mosaic Virus Triple Gene Block Proteins

2009 ◽  
Vol 83 (21) ◽  
pp. 11413-11413 ◽  
Author(s):  
Hyoun-Sub Lim ◽  
Jennifer N. Bragg ◽  
Uma Ganesan ◽  
Steven Ruzin ◽  
Denise Schichnes ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Mosaic Virus ◽  
Triple Gene Block ◽  
Barley Stripe Mosaic Virus ◽  
Gene Block

Barley Stripe Mosaic Virus (Virgaviridae, Hordeivirus) As Etiological Agent of Maize Chlorotic Stripe Disease

2018 ◽  
Vol 44 (2) ◽  
pp. 142-145
Author(s):  
A. V. Gapeka ◽  
A. A. Zelikova ◽  
S. K. Zhmurkina ◽  
V. A. Ledneva ◽  
Yu. G. Volkov ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Etiological Agent ◽  
Barley Stripe Mosaic Virus
Sign in / Sign up

Export Citation Format

Share Document