Comparisons of poa semilatent and barley stripe mosaic viruses

1972 ◽  
Vol 50 (2) ◽  
pp. 263-267 ◽  
Author(s):  
Z. Polák ◽  
J. T. Slykhuis
Keyword(s):  
Mosaic Virus ◽  
Double Diffusion ◽  
Barley Stripe Mosaic Virus ◽  
Diffusion Test ◽  
Partial Protection ◽  
Normal Length ◽  
Local Lesions ◽  
Cross Absorption ◽  
Precipitin Test ◽  
Agropyron Trachycaulum

Poa semilatent virus (PSLV), which caused chlorosis and rapid death of inoculated wheat, infected a number of grasses susceptible to barley stripe mosaic virus (BSMV); but it also infected Agropyron trachycaulum and Poa palustris which were not susceptible to a barley strain or two oat-infecting strains of BSMV. Conversely BSMV caused local lesions on several species of Chenopodium that did not become infected with PSLV. BSMV protected wheat from infection by PSLV, but PSLV caused only partial protection from BSMV.The normal length and thickness of particles in leaf-dip preparations was 161 × 26 mμ for PSLV and 133 × 25 mμ for BSMV.Serological relationship was not indicated by the Ouchterlony agar double-diffusion test or leaf-dip serology. A distant relationship was shown with the microprecipitin test and the ring interface precipitin test. In cross absorption tests the titers of the antisera to the homologous viruses were not reduced by absorption with the heterologous viruses.PSLV and BSMV appear to be distantly related serotypes.

scholarly journals The N-Terminal 85 Amino Acids of the Barley Stripe Mosaic Virus γb Pathogenesis Protein Contain Three Zinc-Binding Motifs

2004 ◽  
Vol 78 (14) ◽  
pp. 7379-7391 ◽  
Author(s):  
Jennifer N. Bragg ◽  
Diane M. Lawrence ◽  
Andrew O. Jackson
Keyword(s):  
Mosaic Virus ◽  
Binding Activity ◽  
Zinc Binding ◽  
Barley Stripe Mosaic Virus ◽  
Deletion Mutants ◽  
Chenopodium Amaranticolor ◽  
Binding Motifs ◽  
Cysteine Rich Protein ◽  
Local Lesions ◽  
Arginine Residues

ABSTRACT Barley stripe mosaic virus RNAγ encodes γb, a cysteine-rich protein that affects pathogenesis. Nine of the eleven cysteines are concentrated in two clusters, designated C1 (residues 1 to 23) and C2 (residues 60 to 85), that are arranged in zinc finger-like motifs. A basic motif (BM) rich in lysine and arginine (residues 19 to 47) resides between the C1 and C2 clusters. We have demonstrated that γb binds zinc and that the C1, BM, and C2 motifs have independent zinc-binding activities. To evaluate the requirements for binding, mutations were introduced into each region. Cysteine residues at positions 7, 9, 10, 19, and 23 in the C1 motif were replaced with serines. In the BM, asparagines were substituted for lysines at positions 26 and 35, glutamine for arginine at position 25, and glycines for arginines at positions 33 and 36. The C2 mutations included cysteine replacements with serines at positions 60, 64, 71, and 81, and a histidine-to-leucine change at position 85. These mutations destroyed zinc-binding activity in each of the isolated motifs. γb derivatives containing mutations in only two of the motifs retained the ability to bind zinc, whereas a γb derivative containing mutations inactivating all three motifs destroyed the ability to bind zinc. Plants inoculated with transcripts containing combinations of the C1, BM, and C2 mutations elicited a “null” phenotype in barley characteristic of γb deletion mutants and also delayed the appearance and reduced the size of local lesions in Chenopodium amaranticolor. These results show that zinc binding of each of the motifs is critical for the biological activity of γb.

DIFFERENTIATION OF AGROPYRON MOSAIC, WHEAT STREAK MOSAIC, AND A HITHERTO UNRECOGNIZED HORDEUM MOSAIC VIRUS IN CANADA

1966 ◽  
Vol 44 (9) ◽  
pp. 1191-1208 ◽  
Author(s):  
J. T. Slykhuis ◽  
W. Bell
Keyword(s):  
Mosaic Virus ◽  
Avena Sativa ◽  
Systemic Infection ◽  
Triticum Aestivum L ◽  
Differential Centrifugation ◽  
Mild Strain ◽  
Serological Tests ◽  
Aceria Tulipae ◽  
Cross Absorption ◽  
Hordeum Jubatum

Agropyron mosaic virus (AMV) from Ontario and wheat streak mosaic virus (WSMV) from Alberta readily infected wheat (Triticum aestivum L.) causing mosaic symptoms that could be differentiated only when carefully compared. All isolates of AMV infected Agropyron repens (L.) Beauv. but not oats (Avena sativa L.). WSMV infected oats but not A. repens. Hordeum mosaic virus (HMV) from Alberta caused mottle rather than streak symptoms on wheat, rye, and other common hosts of WSMV, and did not infect A. repens, but, like AMV, it was not transmitted by Aceria tulipae (K.), the vector of WSMV. It infected Hordeum jubatum L., which is not susceptible to AMV or WSMV. It ranked between AMV and WSMV in longevity and tolerance to heat and pH. The particles were flexuous rods similar to those of WSMV, which appear less flexuous and slightly shorter than particles of AMV.The temperatures at which the viruses multiplied and caused symptoms on wheat ranged from 15° to 33 °C for WSMV and 10° to 30° for AMV. HMV multiplied at all temperatures from 10° to 33 °C but caused symptoms only at 10° to 30 °C.Specific antisera were prepared by partially purifying the viruses by differential centrifugation of juice from diseased wheat, emulsifying the juice with adjuvant, then injecting rabbits intramuscularly. The precipitation titers against their homologous viruses were 1/640, 1/1280, and 1/1280 for the WSMV, AMV, and HMV antisera respectively. The WSMV antiserum did not react with the heterologous viruses, but two HMV antisera had a precipitation titer of 1/16 against AMV, and an AMV antiserum had a titer of 1/10 againt WSMV. Cross absorption of each of the antisera with the heterologous viruses did not reduce the titers against the homologous viruses.The infection of wheat with a mild strain of any of the three viruses protected against later infection by a severe strain of the same virus. Both AMV and HMV were synergistic in combination with WSMV. Serological tests demonstrated that both AMV and HMV multiplied in wheat simultaneously inoculated with both viruses, but systemic infection with either virus protected the plants against later infection by the other.Despite general similarities in physical characteristics and effects on wheat, specific differences in other characteristics show that AMV, HMV, and WSMV are sufficiently different to be designated as different viruses rather than closely related strains of one virus.

The visualization of local lesions caused by the common bean mosaic virus

1972 ◽  
Vol 14 (5) ◽  
pp. 369-370 ◽  
Author(s):  
J. Polák ◽  
J. Chod
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Local Lesions ◽  
The Common

scholarly journals Barley Stripe Mosaic Virus (BSMV) Induced MicroRNA Silencing in Common Wheat (Triticum aestivum L.)

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0126621 ◽  
Author(s):  
Jian Jiao ◽  
Yichun Wang ◽  
Jonathan Nimal Selvaraj ◽  
Fuguo Xing ◽  
Yang Liu
Keyword(s):  
Triticum Aestivum ◽  
Mosaic Virus ◽  
Common Wheat ◽  
Triticum Aestivum L ◽  
Barley Stripe Mosaic Virus

scholarly journals Barley Stripe Mosaic Virus γb Interacts with Glycolate Oxidase and Inhibits Peroxisomal ROS Production to Facilitate Virus Infection

2018 ◽  
Vol 11 (2) ◽  
pp. 338-341 ◽  
Author(s):  
Meng Yang ◽  
Zhenggang Li ◽  
Kun Zhang ◽  
Xuan Zhang ◽  
Yongliang Zhang ◽  
...  
Keyword(s):  
Virus Infection ◽  
Mosaic Virus ◽  
Glycolate Oxidase ◽  
Barley Stripe Mosaic Virus ◽  
Ros Production

scholarly journals Subcellular Localization of the Barley Stripe Mosaic Virus Triple Gene Block Proteins

2009 ◽  
Vol 83 (21) ◽  
pp. 11413-11413 ◽  
Author(s):  
Hyoun-Sub Lim ◽  
Jennifer N. Bragg ◽  
Uma Ganesan ◽  
Steven Ruzin ◽  
Denise Schichnes ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Mosaic Virus ◽  
Triple Gene Block ◽  
Barley Stripe Mosaic Virus ◽  
Gene Block

scholarly journals G7H, a New Soybean mosaic virus Strain: Its Virulence and Nucleotide Sequence of CI Gene

Plant Disease ◽  
2003 ◽  
Vol 87 (11) ◽  
pp. 1372-1375 ◽  
Author(s):  
Yul-Ho Kim ◽  
Ok-Sun Kim ◽  
Bong-Choon Lee ◽  
Jung-Kyung Moon ◽  
Sang-Chul Lee ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Virus Strain ◽  
Soybean Mosaic Virus ◽  
Mosaic Symptom ◽  
Inclusion Region ◽  
Soybean Cultivars ◽  
Soybean Mosaic Virus Strain ◽  
Local Lesions ◽  
Differential Cultivars

A new Soybean mosaic virus (SMV) strain was isolated in Korea and designated as G7H. Its virulence on eight differentials and 42 Korean soybean cultivars was compared with existing SMV strains. G7H caused the same symptoms as G7 did on the eight differential cultivars. However, it caused different symptoms on the G7-immune Korean soybean cultivars; G7H caused necrosis in Suwon 97 (Hwangkeumkong) and Suwon 181 (Daewonkong), and a mosaic symptom in Miryang 41 (Duyoukong), while G7 caused only local lesions on those varieties. The nucleotide sequence of the cylindrical inclusion region of G7H was determined and compared with other SMV strains. G7H shared 96.3 and 91.3% nucleotide similarities with G2 and G7, respectively; whereas G7 shared 95.6% nucleotide similarity with G5H.

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