Influence of Whole-Body γ Irradiation upon Rat Liver Mitochondrial Fractions

1981 ◽  
Vol 88 (2) ◽  
pp. 377 ◽  
Author(s):  
Jean-François Kergonou ◽  
Monique Braquet ◽  
Guy Rocquet ◽  
Jean-Francois Kergonou
Keyword(s):  
Rat Liver ◽  
Whole Body ◽  
Γ Irradiation ◽  
Mitochondrial Fractions

Induced Transport of Amino Acids in Rat Liver after Whole-Body γ Irradiation

1976 ◽  
Vol 68 (1) ◽  
pp. 138 ◽  
Author(s):  
Wayne Flory ◽  
Otto W. Neuhaus
Keyword(s):  
Amino Acids ◽  
Rat Liver ◽  
Whole Body ◽  
Γ Irradiation

Isolation of blood and intracellular forms of Trypanosoma cruzi from rats and other rodents and preliminary studies of their metabolism

Parasitology ◽  
1978 ◽  
Vol 76 (2) ◽  
pp. 159-176 ◽  
Author(s):  
W. E. Gutteridge ◽  
B. Cover ◽  
Maria Gaborak
Keyword(s):  
Trypanosoma Cruzi ◽  
Blood Cells ◽  
Light And Electron Microscopy ◽  
Deae Cellulose ◽  
Whole Body ◽  
Differential Centrifugation ◽  
Γ Irradiation ◽  
Hind Limb Muscle ◽  
Radio Tracer

SummaryIsolation of blood and intracellular forms of Trypanosoma cruzi was made mainly from rats (90–110 g) which had received 580 rad of whole-body γ-irradiation not more than 24 h before subcutaneous inoculation with 107 trypomastigotes of the Sonya strain of T. cruzi. Unirradiated chinchillas (250–350 g) were, however, used for some experiments. Blood forms were isolated using a technique involving differential centrifugation to remove most of the erythrocytes and DEAE–cellulose chromatography to remove the remaining blood cells. Overall recoveries were usually in the range 30–70%. Parasites were mainly (approximately 98%) broad forms and were motile, metabolically active (as judged by respiratory and radio-tracer incorporation studies) and had lost none of their infectivity for mice. Intracellular forms were isolated from hind-limb muscle tissue. This was disrupted in an MSE tissue homogenizer and the homogenate incubated with DNase, collagenase and trypsin. Parasites, contaminated only by a few blood cells, were then obtained by differential centrifugation. For purer preparations, a terminal sucrose gradient step was used. Recoveries ranged between 40 and 70%. About 1–3% of the parasites isolated were epimastigotes and trypomastigotes; the remainder are probably best collectively termed ‘amastigotes’, though they were pointed and most had a short, free flagellum. They were undamaged as judged by light and electron microscopy and metabolically active as judged by respiratory and radio-tracer incorporation studies. However, the infectivity for mice of both these purified preparations and the initial cell homogenates could be accounted for by the epimastigotes and trypomastigotes present in them. Preliminary biochemical studies with isolated parasites have shown that blood, intracellular and culture forms of T. cruzi have a respiratory system which is in part sensitive to CN- and that all forms synthesize nucleic acids and proteins when incubated in vitro. There appears, however, to be a lack of DNA synthesis in blood stages, and thus it is not surprising that these forms do not divide.

Gender dimorphism in rat liver mitochondrial oxidative metabolism and biogenesis

2005 ◽  
Vol 289 (2) ◽  
pp. C372-C378 ◽  
Author(s):  
Roberto Justo ◽  
Jordi Boada ◽  
Margalida Frontera ◽  
Jordi Oliver ◽  
Jordi Bermúdez ◽  
...  
Keyword(s):  
Gender Differences ◽  
Protein Content ◽  
Rat Liver ◽  
Oxidative Metabolism ◽  
Mitochondrial Protein ◽  
Electron Microscopic ◽  
Gender Dimorphism ◽  
Protein Levels ◽  
Mitochondrial Fractions ◽  
Mitochondrial Oxidative Metabolism

In the present study, we have investigated gender differences in rat liver mitochondrial oxidative metabolism. Total mitochondrial population (M) as well as the heavy (M1), medium (M3), and light (M8) mitochondrial fractions obtained by means of differential centrifugation steps at 1,000, 3,000, and 8,000 g, respectively, were isolated. Electron microscopic analysis was performed and mitochondrial protein content and cardiolipin levels, mitochondrial O2 flux, ATP synthase activity, mitochondrial membrane potential, and mitochondrial transcription factor A (TFAM) protein levels were measured in each sample. Our results indicate that mitochondria from females have higher protein content and higher cardiolipin levels, greater respiratory and phosphorylative capacities, and more-energized mitochondria in respiratory state 3. Moreover, protein levels of TFAM were four times greater in females than in males. Gender differences in the aforementioned parameters were more patent in the isolated heavy M1 and M3 mitochondrial fractions. The present study demonstrates that gender-related differences in liver mitochondrial function are due mainly to a higher capacity and efficiency of substrate oxidation, likely related to greater mitochondrial machinery in females than in males, which is in accord with greater mitochondrial differentiation in females.

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