Kinetics in Parasite Abundance in Susceptible and Resistant Mice Infected with an Avirulent Strain of Toxoplasma gondii by Using Quantitative Competitive PCR

1997 ◽  
Vol 83 (6) ◽  
pp. 1070 ◽  
Author(s):  
Wentian Luo ◽  
Fumie Aosai ◽  
Masakatsu Ueda ◽  
Keizo Yamashita ◽  
Kumiko Shimizu ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Avirulent Strain ◽  
Competitive Pcr ◽  
Parasite Abundance

Acute infection with an avirulent strain of Toxoplasma gondii causes decreasing and atrophy of nitrergic myenteric neurons of rats

2017 ◽  
Vol 119 (4) ◽  
pp. 423-427 ◽  
Author(s):  
Débora de Mello Gonçales Sant’Ana ◽  
Marcelo Biondaro Gois ◽  
Catchia Hermes-Uliana ◽  
Letícia Sarturi Pereira-Severi ◽  
Emily Martins Baptista ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Acute Infection ◽  
Avirulent Strain ◽  
Myenteric Neurons

A note on the behaviour of an avirulent strain of Toxoplasma gondii in some B-vitamin deficient chicks

Parasitology ◽  
1961 ◽  
Vol 51 (1-2) ◽  
pp. 265-267 ◽  
Author(s):  
C. Kulasiri ◽  
H. Prasad
Keyword(s):  
Toxoplasma Gondii ◽  
Nicotinic Acid ◽  
Pantothenic Acid ◽  
Avirulent Strain ◽  
Cod Liver Oil ◽  
Parasite Distribution ◽  
Heat Labile ◽  
B Vitamin ◽  
Ordinary Diet

No differences in susceptibility or parasite distribution of avirulent Toxoplasma was observed between biotin-, pantothenic acid-, nicotinic acid-, riboflavin- and heat-labile vitamins-deficient chicks and their controls. Ordinary diet supplemented with cod-liver oil did not have any effect either. Avirulent Toxoplasma were readily isolated from the brains of all the animals at the end of 1 month.We are grateful to Professor P. C. C. Garnham for his interest.

Early Kinetics of Toxoplasma gondii Infection in Mice Infected Orally with Cysts of an Avirulent Strain

1995 ◽  
Vol 81 (2) ◽  
pp. 327 ◽  
Author(s):  
Marie Helene Sumyuen ◽  
Yves Jean Francois Garin ◽  
Francis Derouin
Keyword(s):  
Toxoplasma Gondii ◽  
Avirulent Strain ◽  
Toxoplasma Gondii Infection ◽  
Kinetics Of

Occurrence and genetic characterization of Toxoplasma gondii in naturally infected pigs

2013 ◽  
Vol 58 (3) ◽  
Author(s):  
Ľudmila Turčeková ◽  
Daniela Antolová ◽  
Katarína Reiterová ◽  
František Spišák
Keyword(s):  
Toxoplasma Gondii ◽  
Slovak Republic ◽  
Virulent Strain ◽  
Avirulent Strain ◽  
Genotype I ◽  
Tissue Samples ◽  
Obligate Intracellular ◽  
Wide Range ◽  
Slaughter Pigs

AbstractThe protozoan Toxoplasma gondii is an obligate intracellular parasite that infects a wide range of warm-blooded vertebrates. The data about the occurrence of toxoplasmosis in slaughter pigs in the Slovak Republic are still missing. The aim of our study was to estimate the prevalence of toxoplasmosis in pigs from Slovakia during the period of 2006–2010 by ELISA and PCR methods. In sera of 970 slaughter pigs, 2.16% seropositivity to T. gondii was detected. In tissue samples of seropositive pigs the presence of T. gondii DNA was confirmed. In six monitored Slovak regions the seropositivity varied between 1.11 and 3.48%. The statistically significant differences were recorded between the Košice and Prešov region. The seroprevalence of toxoplasmosis in sows (4.26%) was two times higher than that in slaughter pigs (2.06%) (OR = 2.12; 95% CI = 0.48–9.36). Presence of Toxoplasma gondii in tissues of seropositive pig isolates was confirmed by TGR1E and B1 genes and analysis of DNA polymorphism at SAG2 and ROP1 genes revealed the presence of virulent strain of genotype I in 85.7% of infected pigs and an avirulent strain (genotype II) in 14.3% of pigs.

Protection against Lethal Toxoplasmosis in Mice by an Avirulent Strain of Toxoplasma gondii: Stimulation of IFN-γ and TNF-α Response

1999 ◽  
Vol 93 (4) ◽  
pp. 231-240 ◽  
Author(s):  
Sakhina Haque ◽  
Jacqueline Franck ◽  
Henri Dumon ◽  
Lloyd H. Kasper ◽  
Azizul Haque
Keyword(s):  
Toxoplasma Gondii ◽  
Avirulent Strain ◽  
Tnf Α ◽  
Ifn Γ ◽  
Stimulation Of

Parasite load in pregnant mice infected by Toxoplasma gondii assayed by quantitative competitive-PCR

1997 ◽  
Vol 46 (2) ◽  
pp. 143-147 ◽  
Author(s):  
Na He ◽  
Fumie Aosai ◽  
Wen-Tian Luo ◽  
Masakatsu Ueda ◽  
Tian-Hui Yang ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Parasite Load ◽  
Competitive Pcr ◽  
Pregnant Mice

Membrane localization and demonstration of isoforms of nucleoside triphosphate hydrolase from Toxoplasma gondii

Parasitology ◽  
2001 ◽  
Vol 122 (1) ◽  
pp. 15-23 ◽  
Author(s):  
T. KIKUCHI ◽  
T. FURUTA ◽  
S. KOJIMA
Keyword(s):  
Monoclonal Antibody ◽  
Toxoplasma Gondii ◽  
Virulent Strain ◽  
Surface Membrane ◽  
Vero Cells ◽  
Host Cells ◽  
Nucleoside Triphosphate ◽  
Avirulent Strain ◽  
Potential Candidate ◽  
Dependent Manner

Toxoplasma gondii has a unique enzyme, a NTPase, which has a wide specificity toward NTP. In the present study, we produced a monoclonal antibody (IgG1, 6C6) against the enzyme which could recognize NTPase isozymes among several strains of T. gondii. Three avirulent strains of T. gondii, ME49, Beverley and Nakayama, were found to have 1 NTPase (63kDa, pI 6·0), while a virulent strain RH and an avirulent strain Fukaya had 2 isozymes (63kDa) with different pIs (pIs 6·0 and 6·5 for the former, and pIs 6·2 and 6·4 for the latter, respectively), suggesting that this monoclonal antibody recognizes a common epitope of NTPase among T. gondii strains. Furthermore, 6C6 could inhibit NTPase activity in the presence of dithiothreitol in a dose-dependent manner, and immuno-EM study of NTPase revealed that this molecule is located on the surface membrane of T. gondii tachyzoites. When Vero cells were co-cultured with tachyzoites pre-treated with 6C6, the number of infected cells significantly decreased, suggesting that 6C6 inhibits invasion of the parasites to host cells. These data suggest that the molecule recognized by 6C6 might be considered a potential candidate antigen for vaccines against T. gondii tachyzoites.

scholarly journals Omega-3 Polyunsaturated Fatty Acids Prevent Toxoplasma gondii Infection by Inducing Autophagy via AMPK Activation

Nutrients ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 2137 ◽  
Author(s):  
Choi ◽  
Lee ◽  
Lee ◽  
Park ◽  
Lee ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Toxoplasma Gondii ◽  
Transgenic Mice ◽  
Polyunsaturated Fatty Acids ◽  
Intracellular Survival ◽  
Avirulent Strain ◽  
Omega 3 ◽  
Wild Type ◽  
Murine Bone Marrow ◽  
Toxoplasma Gondii Infection

Omega-3 polyunsaturated fatty acids (ω3-PUFAs) have potential protective activity in a variety of infectious diseases, but their actions and underlying mechanisms in Toxoplasma gondii infection remain poorly understood. Here, we report that docosahexaenoic acid (DHA) robustly induced autophagy in murine bone marrow-derived macrophages (BMDMs). Treatment of T. gondii-infected macrophages with DHA resulted in colocalization of Toxoplasma parasitophorous vacuoles with autophagosomes and reduced intracellular survival of T. gondii. The autophagic and anti-Toxoplasma effects induced by DHA were mediated by AMP-activated protein kinase (AMPK) signaling. Importantly, BMDMs isolated from Fat-1 transgenic mice, a well-known animal model capable of synthesizing ω3-PUFAs from ω6-PUFAs, showed increased activation of autophagy and AMPK, leading to reduced intracellular survival of T. gondii when compared with wild-type BMDMs. Moreover, Fat-1 transgenic mice exhibited lower cyst burden in the brain following infection with the avirulent strain ME49 than wild-type mice. Collectively, our results revealed mechanisms by which endogenous ω3-PUFAs and DHA control T. gondii infection and suggest that ω3-PUFAs might serve as therapeutic candidate to prevent toxoplasmosis and infection with other intracellular protozoan parasites.

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