The Use of Tissue Culture-Grown Trophozoites of an Avirulent Strain of Toxoplasma gondii for the Immunization of Mice and Guinea Pigs

1971 ◽  
Vol 57 (2) ◽  
pp. 386 ◽  
Author(s):  
J. L. Krahenbuhl ◽  
A. A. Blazkovec ◽  
M. G. Lysenko
Keyword(s):  
Tissue Culture ◽  
Toxoplasma Gondii ◽  
Guinea Pigs ◽  
Avirulent Strain

Acute infection with an avirulent strain of Toxoplasma gondii causes decreasing and atrophy of nitrergic myenteric neurons of rats

2017 ◽  
Vol 119 (4) ◽  
pp. 423-427 ◽  
Author(s):  
Débora de Mello Gonçales Sant’Ana ◽  
Marcelo Biondaro Gois ◽  
Catchia Hermes-Uliana ◽  
Letícia Sarturi Pereira-Severi ◽  
Emily Martins Baptista ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Acute Infection ◽  
Avirulent Strain ◽  
Myenteric Neurons

scholarly journals Use of quantitative real-time PCR for studying the dissemination of Leptospira interrogans in the guinea pig infection model of leptospirosis

2009 ◽  
Vol 58 (5) ◽  
pp. 648-655 ◽  
Author(s):  
Kristel Lourdault ◽  
Florence Aviat ◽  
Mathieu Picardeau
Keyword(s):  
Guinea Pig ◽  
Real Time ◽  
Real Time Pcr ◽  
Guinea Pigs ◽  
Infection Model ◽  
Avirulent Strain ◽  
Leptospira Interrogans ◽  
Quantitative Real Time Pcr ◽  
Guinea Pig Model

The dynamics of leptospirosis infection have been poorly studied. The purpose of this study was to determine the LD50, rate of bacterial dissemination, histopathology and antibody responses against leptospira following inoculation with the highly virulent Leptospira interrogans Fiocruz L1-130 strain in a guinea pig model of leptospirosis. Three routes of infection (intraperitoneal, conjunctival and subcutaneous inoculation) were used to establish disease in guinea pigs. The size and kinetics of leptospiral burdens in the blood and tissues of infected animals were determined over a 1 week course of infection using quantitative real-time PCR (qPCR). Bacteraemia peaked at day 5 post-infection reaching more than 5×104 leptospires ml−1. The highest spirochaetal load was found in the liver and kidneys, and was associated with alterations in organ tissues and a decline in liver and kidney functions. In contrast, lesions and bacteria were not detected in guinea pigs infected with an avirulent strain derived from a high-passage-number in vitro-passaged variant of the Fiocruz L1-130 strain. The use of qPCR supports the findings of earlier studies and provides an easy and reliable method for the quantification of L. interrogans in the tissues of infected animals. qPCR will be used in future studies to evaluate the efficacy of vaccine candidates against leptospirosis and the virulence of selected L. interrogans mutants relative to the parental strain.

scholarly journals THE MULTIPLICATION OF TUBERCLE BACILLI WITHIN NORMAL PHAGOCYTES IN TISSUE CULTURE

1952 ◽  
Vol 96 (2) ◽  
pp. 137-150 ◽  
Author(s):  
Emanuel Suter
Keyword(s):  
Tissue Culture ◽  
Culture Medium ◽  
Mononuclear Cells ◽  
Virulent Strain ◽  
Host Cells ◽  
Avirulent Strain ◽  
Glass Slides ◽  
Intracellular Multiplication ◽  
Cultivation In Vitro

A technique has been described for the cultivation in vitro of normal mononuclear cells on glass slides in a liquid medium. Under these conditions the monocytes transformed into macrophages which proliferated as in ordinary tissue culture. These cultures of monocytes could be infected with tubercle bacilli. The numbers of stainable tubercle bacilli within the monocytes increased steadily in cultures infected with virulent or attenuated strains. Evidence is given to support the view that this increase in numbers of bacilli was due to intracellular multiplication. There was no evidence of intracellular bacillary multiplication in cultures infected with an avirulent strain. Tubercle bacilli multiplying within phagocytes in vitro exert a damaging effect upon the host cells. The damage was most obvious in cells infected with a virulent strain. Tubercle bacilli within phagocytes were protected against the bacteriostatic effect of streptomycin added in a concentration of 5 γ per ml. of culture medium. This permitted the use of streptomycin in infected cultures to prevent extracellular multiplication of the bacilli.

scholarly journals Studies on Thrombopoiesis

Blood ◽  
1957 ◽  
Vol 12 (6) ◽  
pp. 507-519 ◽  
Author(s):  
G. IZAK ◽  
D. NELKEN ◽  
J. GUREVITCH ◽  
MISS A. HERZOG
Keyword(s):  
Bone Marrow ◽  
Tissue Culture ◽  
Guinea Pigs ◽  
Gradual Increase ◽  
Bone Marrow Tissue ◽  
Experimental Animals ◽  
Healthy Humans ◽  
The One

Abstract Thrombocyte production from megakaryocytes of healthy humans, dogs, guinea pigs and mice was observed continuously for one to six days in tissue culture. Approximately 70 per cent of the explanted megakaryocytes broke down to give rise to numerous platelets, while the remaining 30 per cent of the cells remained unchanged. The newly formed thrombocytes were separated from the rest of the bone marrow tissue, counted and their serotonin absorbing capacity determined. There was invariably a gradual increase in both the number of thrombocytes and in their serotonin absorbing capacity during the one to six days of observation. The results obtained were similar in human megakaryocytes and in those of experimental animals.

Kinetics in Parasite Abundance in Susceptible and Resistant Mice Infected with an Avirulent Strain of Toxoplasma gondii by Using Quantitative Competitive PCR

1997 ◽  
Vol 83 (6) ◽  
pp. 1070 ◽  
Author(s):  
Wentian Luo ◽  
Fumie Aosai ◽  
Masakatsu Ueda ◽  
Keizo Yamashita ◽  
Kumiko Shimizu ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Avirulent Strain ◽  
Competitive Pcr ◽  
Parasite Abundance

Freeze Preservation of Tissue Culture Propagated Toxoplasma gondii

1974 ◽  
Vol 60 (2) ◽  
pp. 368 ◽  
Author(s):  
Robert O. Bollinger ◽  
Nabil Musallam ◽  
Cyril S. Stulberg
Keyword(s):  
Tissue Culture ◽  
Toxoplasma Gondii

scholarly journals TISSUE CULTURE STUDIES ON BACTERIAL ALLERGY IN EXPERIMENTAL BRUCELLOSIS

1958 ◽  
Vol 107 (2) ◽  
pp. 319-332 ◽  
Author(s):  
Dorothy H. Heilman ◽  
Dexter H. Howard ◽  
Charles M. Carpenter
Keyword(s):  
Tissue Culture ◽  
Guinea Pigs ◽  
Protective Action ◽  
Tissue Cultures ◽  
Culture Methods ◽  
Culture Studies ◽  
Normal Cells ◽  
Specific Antibodies ◽  
Splenic Cells ◽  
Homologous Antiserum

Tissue culture methods have been used to investigate infectious allergy in experimental brucellosis. A study was made of the effect of whole cell Brucella antigen on cultures of spleen from normal and Brucella-infected guinea pigs. The degree of toxicity was based upon the inhibition of migration of wandering cells and upon the morphologic appearance of stained sections of tissue cultures at different periods of incubation. A suspension of heat-killed Br. suis was more toxic for splenic cells from guinea pigs infected with Br. suis than for normal splenic cells. Macrophages were more sensitive than leucocytes to the toxic action of the antigen. The degenerative changes observed in Brucella-sensitive cells exposed to the antigen were similar to the degeneration previously observed in cultures of tuberculin-sensitive cells in the presence of tuberculin. The specific toxicity of the whole Brucella antigen, however, was more marked than that of tuberculin. Preliminary experiments indicate that serum and plasma containing specific antibodies obtained from Brucella-infected guinea pigs reduce the toxic effect of the antigen in cultures of both normal and Brucella-sensitive cells. The protective action of the homologous antiserum was greater for Brucella-sensitive cells than for normal cells.

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