Factors Contributing to the In vitro Development of Ascaris suum from Second-Stage Larvae to Mature Adults

1983 ◽  
Vol 69 (3) ◽  
pp. 549 ◽  
Author(s):  
F. W. Douvres ◽  
J. F. Urban
Keyword(s):  
Ascaris Suum ◽  
In Vitro Development ◽  
Mature Adults ◽  
Second Stage ◽  
Vitro Development

In vitro development of the nematode Contracaecum osculatum Rudolphi 1802 (Nematoda: Anisakinae)

1974 ◽  
Vol 52 (7) ◽  
pp. 847-855 ◽  
Author(s):  
G. McClelland ◽  
K. Ronald
Keyword(s):  
Gastric Mucosa ◽  
Infective Stage ◽  
In Vitro Development ◽  
Second Stage ◽  
Infective Larvae ◽  
Contracaecum Osculatum ◽  
Vitro Development

Eggs of Contracaecum osculatum were dissected from the vagina and uterus of adult worms from seals and were incubated in seawater at 15C. Freshly hatched larvae were cultivated to preadult in Eagle's medium (MEM) with 20% foetal calf serum.At 15C, the ensheathed second-stage larvae exsheathed and grew to the infective stage without further molts. Infective larvae of C. osculatum were 6.5 mm (5.9–9.5) in length after 32 weeks.At 35C, 58% of infective larvae > 2 mm in length molted to subadults which possessed cuticular lips but lacked interlabia. Subadults were 2.9–13.8 mm in length and morphologically similar to larvae found attached to the gastric mucosa of seals. Four subadults subsequently molted to preadults 13.1–17.2 mm in length with interlabia. However, an attempt lo infect a seal with cultivated infective larvae was unsuccessful.

In vitro development of human triploid zygotes reconstructed by pronuclear transfer

2002 ◽  
Vol 78 ◽  
pp. S180-S181
Author(s):  
John Zhang ◽  
Yi Ming Shu ◽  
Lewis C Krey ◽  
Hui Liu ◽  
Guang Lun Zhuang ◽  
...  
Keyword(s):  
In Vitro Development ◽  
Pronuclear Transfer ◽  
Vitro Development

Gallic acid promotes the in vitro development of sheep secondary isolated follicles involving the phosphatidylinositol 3-kinase pathway

2021 ◽  
pp. 106767
Author(s):  
Gizele A.L. Silva ◽  
Luana B. Araújo ◽  
Larissa C.R. Silva ◽  
Bruna B. Gouveia ◽  
Ricássio S. Barberino ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Phosphatidylinositol 3 Kinase ◽  
In Vitro Development ◽  
Vitro Development ◽  
Kinase Pathway ◽  
Phosphatidylinositol 3

scholarly journals Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets

2021 ◽  
Vol 22 (16) ◽  
pp. 8367
Author(s):  
Hien Lau ◽  
Shiri Li ◽  
Nicole Corrales ◽  
Samuel Rodriguez ◽  
Mohammadreza Mohammadi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Oxygen Consumption ◽  
Insulin Secretion ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
In Vitro Development ◽  
Porcine Islets ◽  
Vitro Development

Pre-weaned porcine islets (PPIs) represent an unlimited source for islet transplantation but are functionally immature. We previously showed that necrostatin-1 (Nec-1) immediately after islet isolation enhanced the in vitro development of PPIs. Here, we examined the impact of Nec-1 on the in vivo function of PPIs after transplantation in diabetic mice. PPIs were isolated from pancreata of 8–15-day-old, pre-weaned pigs and cultured in media alone, or supplemented with Nec-1 (100 µM) on day 0 or on day 3 of culture (n = 5 for each group). On day 7, islet recovery, viability, oxygen consumption rate, insulin content, cellular composition, insulin secretion capacity, and transplant outcomes were evaluated. While islet viability and oxygen consumption rate remained high throughout 7-day tissue culture, Nec-1 supplementation on day 3 significantly improved islet recovery, insulin content, endocrine composition, GLUT2 expression, differentiation potential, proliferation capacity of endocrine cells, and insulin secretion. Adding Nec-1 on day 3 of tissue culture enhanced the islet recovery, proportion of delta cells, beta-cell differentiation and proliferation, and stimulation index. In vivo, this leads to shorter times to normoglycemia, better glycemic control, and higher circulating insulin. Our findings identify the novel time-dependent effects of Nec-1 supplementation on porcine islet quantity and quality prior to transplantation.

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