In vitro Development of Ascaris suum from Third- to Fourth-Stage Larvae and Detection of Metabolic Antigens in Multi-Well Culture Systems

1981 ◽  
Vol 67 (6) ◽  
pp. 800 ◽  
Author(s):  
Joseph F. Urban ◽  
Frank W. Douvres
Keyword(s):  
Ascaris Suum ◽  
Fourth Stage ◽  
In Vitro Development ◽  
Culture Systems ◽  
Vitro Development

In vitro development of cyathostomin larvae from the third stage larvae to the fourth stage: morphologic characterization, effects of refrigeration, and species-specific patterns

2009 ◽  
Vol 163 (4) ◽  
pp. 348-356 ◽  
Author(s):  
Emanuele Brianti ◽  
Salvatore Giannetto ◽  
Donato Traversa ◽  
Sharon R. Chirgwin ◽  
Krishna Shakya ◽  
...  
Keyword(s):  
Fourth Stage ◽  
In Vitro Development ◽  
The Third ◽  
Third Stage ◽  
Species Specific ◽  
Vitro Development

279 IN VITRO DEVELOPMENT IN THREE CULTURE SYSTEMS OF BOVINE OOCYTES FERTILIZED WITH SEX-SORTED SPERM

2011 ◽  
Vol 23 (1) ◽  
pp. 237
Author(s):  
B. Trigal ◽  
E. Gomez ◽  
M. Muñoz ◽  
J.N. Caamaño ◽  
S. Carrocera ◽  
...  
Keyword(s):  
Culture System ◽  
Final Analysis ◽  
Vero Cells ◽  
Bovine Embryos ◽  
Vitro Fertilization ◽  
In Vitro Development ◽  
Culture Systems ◽  
Cell Counts ◽  
Vitro Development

Following in vitro fertilization and culture, the timing of both embryonic cleavage and appearance of blastocysts has been reported to be altered when using sorted spermatozoa (Lu et al. 1999 Theriogenology 52, 1393–1405). To optimize an in vitro culture system for bovine embryos produced with sexed sperm, sorted and unsorted (control) semen samples of 5 bulls (semen samples provided by XY Inc., Fort Collins, CO, USA) were separated using a Percoll gradient followed by a washing step with Fert-TALP medium (Trigal et al. 2010 Reprod. Domest. Anim. 45, 83). The obtained pellet was used for fertilizing in vitro matured cumulus–oocyte complexes from slaughterhouse ovaries. Zygotes were cultured in 1) Vero cells monolayer + B2 + 10% FCS; 2) SOFaaci + 10% FCS; or 3) SOFaaci + 6 g L–1 BSA. A total of 1980 oocytes were in vitro matured and fertilized. Development was recorded on Days 3 and Days 6 to 8, and analysed by ANOVA and Duncan test. Bull, replicate, and culture effects were nonsignificant, and consequently were not considered in the final analysis. Data are percentages of development related to the zygotes cultured, and are expressed as LSM ± SE (see Table 1). The use of sexed sperm significantly reduced development rates compared with unsorted sperm. Thus, sorted sperm showed reduced 5- to 8-cell, Day-6 morulae, and Day-8 blastocyst rates over unsorted sperm within the 3 culture systems analysed. Culture in SOF + BSA was the least efficient embryo culture system in terms of Day-7 blastocyst rates, both with unsorted and sorted sperm. Day-7 blastocyst rates produced with unsorted sperm after culture in Vero cells or SOF + FCS were significantly higher than those obtained after culture in SOF + BSA. Day-7 blastocyst rates obtained with sorted sperm and cultured in Vero cells or SOF + FCS were comparable to those obtained with unsorted sperm in SOF + BSA. No differences were detected between male- and female-sorted sperm (data not shown). Sorted sperm is an effective tool for producing sex-known embryos. Quality assessment (differential cell counts and cryosurvival) of embryos produced with sorted sperm under different conditions are being undertaken. Table 1.In vitro development of bovine embryos fertilized with either untreated or sorted sperm and cultured in Vero cells, SOF + FCS, or SOF + BSA Supported by INIA RTA2008.#0082. M. Muñoz, B. Trigal and D. Martin are sponsored by RYC08-03454, Cajastur and PTA2007-#0268-I. We acknowledge Sexing Technologies for collaboration.

Effects of Retinol on the in vitro Development of Bos Indicus Embryos to Blastocysts in Two Different Culture Systems

2004 ◽  
Vol 39 (5) ◽  
pp. 356-360 ◽  
Author(s):  
PF Lima ◽  
MAL Oliveira ◽  
PBD Gonçalves ◽  
MM Montagner ◽  
H-D Reichenbach ◽  
...  
Keyword(s):  
Bos Indicus ◽  
In Vitro Development ◽  
Culture Systems ◽  
Vitro Development

In vitro development of bovine one-cell embryos fertilized in vitro in serum- and feeder cell-free culture systems

1996 ◽  
Vol 9 (5) ◽  
pp. 583-590 ◽  
Author(s):  
S. Ohboshi ◽  
K. Hanada ◽  
J. Zhao ◽  
M. Hattori ◽  
N. Fujihara ◽  
...  
Keyword(s):  
Feeder Cell ◽  
In Vitro Development ◽  
Culture Systems ◽  
Vitro Development
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