scholarly journals Ascaris suum: Influence of Egg Density on in Vitro Development from Embryonated Egg to Infective Stage

1990 ◽  
Vol 31 (4) ◽  
pp. 489-491
Author(s):  
Lis Eriksen
Keyword(s):  
Ascaris Suum ◽  
Infective Stage ◽  
In Vitro Development ◽  
Egg Density ◽  
Vitro Development

In vitro development of Terranova decipiens (Nematoda) (Krabbe, 1878)

1974 ◽  
Vol 52 (4) ◽  
pp. 471-479 ◽  
Author(s):  
G. McClelland ◽  
K. Ronald
Keyword(s):  
Developmental Stages ◽  
Infective Stage ◽  
In Vitro Development ◽  
Infective Larvae ◽  
Complete Development ◽  
Vitro Development

Eggs of Terranova decipiens were dissected from the vagina and uterus of adult worms from seals and incubated in seawater at 15C. Freshly hatched larvae were cultivated to preadults in Eagle's medium (MEM) with 20% fetal-calf serum.At 15C, 70% of the larvae exsheathed and grew to the infective stage without further molts. Infective larvae were 31.3 (27.7–33.3) mm in length after 52 weeks of cultivation and were similar in morphology to larvae from fish.At 35C, 80%. of cultivated larvae (5.0–24.2 mm in length) molted to preadult. There was a 25% increase in length over a 6-week, period. Cultivated preadults (6.1–36.8 mm in length) were morphologically similar to preadults from seals. They did not, however, complete development to adulthood, and an attempt to infect a seal with cultivated infective larvae was unsuccessful.The described in vitro developmental stages of T. decipiens form a useful guide to the in vivo identification of this important economic nematode.

In vitro development of the nematode Contracaecum osculatum Rudolphi 1802 (Nematoda: Anisakinae)

1974 ◽  
Vol 52 (7) ◽  
pp. 847-855 ◽  
Author(s):  
G. McClelland ◽  
K. Ronald
Keyword(s):  
Gastric Mucosa ◽  
Infective Stage ◽  
In Vitro Development ◽  
Second Stage ◽  
Infective Larvae ◽  
Contracaecum Osculatum ◽  
Vitro Development

Eggs of Contracaecum osculatum were dissected from the vagina and uterus of adult worms from seals and were incubated in seawater at 15C. Freshly hatched larvae were cultivated to preadult in Eagle's medium (MEM) with 20% foetal calf serum.At 15C, the ensheathed second-stage larvae exsheathed and grew to the infective stage without further molts. Infective larvae of C. osculatum were 6.5 mm (5.9–9.5) in length after 32 weeks.At 35C, 58% of infective larvae > 2 mm in length molted to subadults which possessed cuticular lips but lacked interlabia. Subadults were 2.9–13.8 mm in length and morphologically similar to larvae found attached to the gastric mucosa of seals. Four subadults subsequently molted to preadults 13.1–17.2 mm in length with interlabia. However, an attempt lo infect a seal with cultivated infective larvae was unsuccessful.

In vitro development of human triploid zygotes reconstructed by pronuclear transfer

2002 ◽  
Vol 78 ◽  
pp. S180-S181
Author(s):  
John Zhang ◽  
Yi Ming Shu ◽  
Lewis C Krey ◽  
Hui Liu ◽  
Guang Lun Zhuang ◽  
...  
Keyword(s):  
In Vitro Development ◽  
Pronuclear Transfer ◽  
Vitro Development

Gallic acid promotes the in vitro development of sheep secondary isolated follicles involving the phosphatidylinositol 3-kinase pathway

2021 ◽  
pp. 106767
Author(s):  
Gizele A.L. Silva ◽  
Luana B. Araújo ◽  
Larissa C.R. Silva ◽  
Bruna B. Gouveia ◽  
Ricássio S. Barberino ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Phosphatidylinositol 3 Kinase ◽  
In Vitro Development ◽  
Vitro Development ◽  
Kinase Pathway ◽  
Phosphatidylinositol 3
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