A Simplified Cell-Culture Technique for the Serum-Neutralization Test of Infectious Bronchitis

1972 ◽  
Vol 16 (3) ◽  
pp. 512
Author(s):  
A. El Zein ◽  
V. Ghoraib ◽  
S. Nehme ◽  
K. V. Singh ◽  
H. P. Chu
Keyword(s):  
Cell Culture ◽  
Neutralization Test ◽  
Culture Technique ◽  
Infectious Bronchitis ◽  
Serum Neutralization ◽  
Serum Neutralization Test ◽  
Cell Culture Technique

A Simplified Technique for the Serum-Neutralization Test in Infectious Bronchitis

1963 ◽  
Vol 7 (2) ◽  
pp. 203 ◽  
Author(s):  
M. P. Fontaine ◽  
D. Chabas ◽  
M. Fontaine ◽  
A. J. Brion
Keyword(s):  
Neutralization Test ◽  
Infectious Bronchitis ◽  
Serum Neutralization ◽  
Serum Neutralization Test

A Cell Culture Technique for Chromosome Preparation in Cyprinid Fishes

Copeia ◽  
1984 ◽  
Vol 1984 (1) ◽  
pp. 232 ◽  
Author(s):  
Chris T. Amemiya ◽  
John W. Bickham ◽  
John R. Gold
Keyword(s):  
Cell Culture ◽  
Culture Technique ◽  
Chromosome Preparation ◽  
A Cell ◽  
Cyprinid Fishes ◽  
Cell Culture Technique

scholarly journals The development and application of an indirect Elisa test for the detection of antibodies to bovine respiratory syncytial virus in blood serum 

2001 ◽  
Vol 46 (No. 2) ◽  
pp. 29-34 ◽  
Author(s):  
K. Kovařčík
Keyword(s):  
Respiratory Syncytial Virus ◽  
Neutralization Test ◽  
Elisa Test ◽  
Bovine Respiratory Syncytial Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Neutralization ◽  
Specificity And Sensitivity ◽  
Reference Serum ◽  
Serum Neutralization Test ◽  
Syncytial Virus

We developed an indirect enzyme-linked immunosorbent assay (ELISA) for detection of serum antibodies to bovine respiratory syncytial virus. For evaluation of the newly developed ELISA, field sera collected from 549 head of cattle in the Czech Republic were tested in parallel by a serum neutralization test. The tests showed 98.36% agreement. The specificity and sensitivity of the ELISA relative to serum neutralization test was 97.00% (226/233) and 99.37% (314/316), respectively. Tissue culture-grown viral antigen was used in the tests. The corrected optical density (COD) of each sample tested at dilution 1/100 was expressed as a percentage of the COD of a positive reference serum included on each plate, this value was the sample/positive (S/P) ratio. We determined the relationship between the S/P ratio (%) obtained at a dilution 1/100 and the end point titer calculated by serum neutralization test (r = 0.9743). The ELISA test was evaluated by testing acute and convalescent (3 wk later) serum pairs from 9 head of cattle with confirmed BRSV infection for demonstration of seroconversion. The ELISA test demonstrated a clear increase of the S/P ratio (%) between acute and convalescent serum pairs (on average 42.2 ± 13.1).

Sign in / Sign up

Export Citation Format

Share Document