A Simplified Technique for the Serum-Neutralization Test in Infectious Bronchitis

1963 ◽  
Vol 7 (2) ◽  
pp. 203 ◽  
Author(s):  
M. P. Fontaine ◽  
D. Chabas ◽  
M. Fontaine ◽  
A. J. Brion
Keyword(s):  
Neutralization Test ◽  
Infectious Bronchitis ◽  
Serum Neutralization ◽  
Serum Neutralization Test

scholarly journals The development and application of an indirect Elisa test for the detection of antibodies to bovine respiratory syncytial virus in blood serum 

2001 ◽  
Vol 46 (No. 2) ◽  
pp. 29-34 ◽  
Author(s):  
K. Kovařčík
Keyword(s):  
Respiratory Syncytial Virus ◽  
Neutralization Test ◽  
Elisa Test ◽  
Bovine Respiratory Syncytial Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Neutralization ◽  
Specificity And Sensitivity ◽  
Reference Serum ◽  
Serum Neutralization Test ◽  
Syncytial Virus

We developed an indirect enzyme-linked immunosorbent assay (ELISA) for detection of serum antibodies to bovine respiratory syncytial virus. For evaluation of the newly developed ELISA, field sera collected from 549 head of cattle in the Czech Republic were tested in parallel by a serum neutralization test. The tests showed 98.36% agreement. The specificity and sensitivity of the ELISA relative to serum neutralization test was 97.00% (226/233) and 99.37% (314/316), respectively. Tissue culture-grown viral antigen was used in the tests. The corrected optical density (COD) of each sample tested at dilution 1/100 was expressed as a percentage of the COD of a positive reference serum included on each plate, this value was the sample/positive (S/P) ratio. We determined the relationship between the S/P ratio (%) obtained at a dilution 1/100 and the end point titer calculated by serum neutralization test (r = 0.9743). The ELISA test was evaluated by testing acute and convalescent (3 wk later) serum pairs from 9 head of cattle with confirmed BRSV infection for demonstration of seroconversion. The ELISA test demonstrated a clear increase of the S/P ratio (%) between acute and convalescent serum pairs (on average 42.2 ± 13.1).

A rapid serum neutralization test in microplates for the detection of antibodies to hog cholera virus

1989 ◽  
Vol 23 (1) ◽  
pp. 77-79 ◽  
Author(s):  
C. Buonavoglia ◽  
E. Falcone ◽  
S. Pestalozza ◽  
L. Di Trani ◽  
E. D'Amore
Keyword(s):  
Neutralization Test ◽  
Serum Neutralization ◽  
Hog Cholera ◽  
Hog Cholera Virus ◽  
Serum Neutralization Test

scholarly journals Comparison of the Serum Neutralization Test and a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to Vesicular Stomatitis Virus New Jersey and Vesicular Stomatitis Virus Indiana

2002 ◽  
Vol 14 (3) ◽  
pp. 240-242 ◽  
Author(s):  
Juan Francisco Alvarado ◽  
Gaby Dolz ◽  
Marco V. Herrero ◽  
Brian McCluskey ◽  
Mo Salman
Keyword(s):  
New Jersey ◽  
Confidence Interval ◽  
Vesicular Stomatitis Virus ◽  
Immunosorbent Assay ◽  
Neutralization Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Serum Neutralization ◽  
Serum Neutralization Test ◽  
Vesicular Stomatitis

A competitive enzyme-linked immunosorbent assay (C-ELISA) for the detection of antibodies against vesicular stomatitis virus New Jersey (VSV-NJ) and vesicular stomatitis virus Indiana (VSV-IN) was compared with the serum neutralization test (SNT) using 1,106 serum samples obtained from dairy cattle on sentinel study farms in the Poás region of Costa Rica. Kappa coefficients between the C-ELISA and the SNT were 0.8871 (95% confidence interval [CI]: 0.8587–0.9155) and 0.6912 (95% CI: 0.6246–0.7577) for the VSV-NJ and VSV-IN tests, respectively. These results indicate good to excellent agreement between the 2 tests under these conditions.

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