scholarly journals Comparison of an enzyme-linked immunosorbent assay with serum neutralization test for serodiagnosis of porcine epidemic diarrhea virus infection

2005 ◽  
Vol 6 (4) ◽  
pp. 349 ◽  
Author(s):  
Jin Sik Oh ◽  
Dae Sub Song ◽  
Jeong Sun Yang ◽  
Ju Young Song ◽  
Hyoung Joon Moon ◽  
...  
Keyword(s):  
Virus Infection ◽  
Immunosorbent Assay ◽  
Porcine Epidemic Diarrhea Virus ◽  
Neutralization Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diarrhea Virus ◽  
Serum Neutralization ◽  
Serum Neutralization Test ◽  
Porcine Epidemic Diarrhea

scholarly journals Comparison of the Serum Neutralization Test and a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to Vesicular Stomatitis Virus New Jersey and Vesicular Stomatitis Virus Indiana

2002 ◽  
Vol 14 (3) ◽  
pp. 240-242 ◽  
Author(s):  
Juan Francisco Alvarado ◽  
Gaby Dolz ◽  
Marco V. Herrero ◽  
Brian McCluskey ◽  
Mo Salman
Keyword(s):  
New Jersey ◽  
Confidence Interval ◽  
Vesicular Stomatitis Virus ◽  
Immunosorbent Assay ◽  
Neutralization Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Serum Neutralization ◽  
Serum Neutralization Test ◽  
Vesicular Stomatitis

A competitive enzyme-linked immunosorbent assay (C-ELISA) for the detection of antibodies against vesicular stomatitis virus New Jersey (VSV-NJ) and vesicular stomatitis virus Indiana (VSV-IN) was compared with the serum neutralization test (SNT) using 1,106 serum samples obtained from dairy cattle on sentinel study farms in the Poás region of Costa Rica. Kappa coefficients between the C-ELISA and the SNT were 0.8871 (95% confidence interval [CI]: 0.8587–0.9155) and 0.6912 (95% CI: 0.6246–0.7577) for the VSV-NJ and VSV-IN tests, respectively. These results indicate good to excellent agreement between the 2 tests under these conditions.

scholarly journals Development of a High-Throughput Serum Neutralization Test Using Recombinant Pestiviruses Possessing a Small Reporter Tag

Pathogens ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 188 ◽  
Author(s):  
Madoka Tetsuo ◽  
Keita Matsuno ◽  
Tomokazu Tamura ◽  
Takasuke Fukuhara ◽  
Taksoo Kim ◽  
...  
Keyword(s):  
High Throughput ◽  
Neutralization Test ◽  
Classical Swine Fever ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Border Disease Virus ◽  
Diarrhea Virus ◽  
Serum Neutralization ◽  
Border Disease ◽  
Serum Neutralization Test

A serum neutralization test (SNT) is an essential method for the serological diagnosis of pestivirus infections, including classical swine fever, because of the cross reactivity of antibodies against pestiviruses and the non-quantitative properties of antibodies in an enzyme-linked immunosorbent assay. In conventional SNTs, an immunoperoxidase assay or observation of cytopathic effect after incubation for 3 to 7 days is needed to determine the SNT titer, which requires labor-intensive or time-consuming procedures. Therefore, a new SNT, based on the luciferase system and using classical swine fever virus, bovine viral diarrhea virus, and border disease virus possessing the 11-amino-acid subunit derived from NanoLuc luciferase was developed and evaluated; this approach enabled the rapid and easy determination of the SNT titer using a luminometer. In the new method, SNT titers can be determined tentatively at 2 days post-infection (dpi) and are comparable to those obtained by conventional SNTs at 3 or 4 dpi. In conclusion, the luciferase-based SNT can replace conventional SNTs as a high-throughput antibody test for pestivirus infections.

Development and application of an indirect enzyme-linked immunosorbent assay using recombinant S1 for serological testing of porcine epidemic diarrhea virus

2019 ◽  
Vol 65 (5) ◽  
pp. 343-352
Author(s):  
Ying Shan ◽  
Yajie Liu ◽  
Ziqi Liu ◽  
Guowei Li ◽  
Cong Chen ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Porcine Epidemic Diarrhea Virus ◽  
Fluorescent Antibody ◽  
Area Under The Curve ◽  
Antibody Detection ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

Porcine epidemic diarrhea virus (PEDV) causes severe infectious diseases in all ages of swine and leads to serious economic losses. Serologic tests are widely accepted and used to detect anti-PEDV antibodies that could indicate PEDV infection or vaccination. In this study, PEDV recombinant S1 protein (rS1) was expressed with the Bac-to-Bac system and purified by nickel-affinity chromatography. An indirect enzyme-linked immunosorbent assay based on rS1 (rS1-ELISA) was then developed and optimized by checkerboard assays with serial dilutions of antigen and serum. Serum samples from 453 domestic pigs and 42 vaccinated pigs were analyzed by the indirect fluorescent antibody (IFA) test and rS1-ELISA. Taking IFA as a gold standard, rS1-ELISA produced a high sensitivity (90.7%) and specificity (94.6%) by a receiver operating characteristic (ROC) curve. In addition, ROC analysis also revealed that rS1-ELISA was consistent with IFA (area under the curve 0.9583 ± 0.0082). This rS1-ELISA was then applied to antibody detection in inactivated PEDV vaccinated pigs. The antibody could be detected 2–4 weeks after the first inoculation. These results indicated that the rS1-ELISA established in this study provides a promising and reliable tool for serologic detection of anti-PEDV IgG antibodies in infected or vaccinated pigs.

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