Dormancy Release and Development from Gemmules of the Freshwater Sponge, "Spongilla lacustris" L.: A Supravital Study with Acridine Orange

BioScience ◽  
1984 ◽  
Vol 34 (4) ◽  
pp. 259-260
Author(s):  
Frederick W. Harrison ◽  
Ronald R. Cowden
Keyword(s):  
Acridine Orange ◽  
Dormancy Release ◽  
Freshwater Sponge

Vacuoles Induced in Mammalian Cells by Helicobacter Cytotoxin are Acidic Organelles

1990 ◽  
Vol 48 (3) ◽  
pp. 168-169
Author(s):  
M. H. Chestnut ◽  
C. E. Catrenich
Keyword(s):  
Acridine Orange ◽  
Mammalian Cells ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ulcer Disease ◽  
Gastroduodenal Disease ◽  
H Pylori

Helicobacter pylori is a non-invasive, Gram-negative spiral bacterium first identified in 1983, and subsequently implicated in the pathogenesis of gastroduodenal disease including gastritis and peptic ulcer disease. Cytotoxic activity, manifested by intracytoplasmic vacuolation of mammalian cells in vitro, was identified in 55% of H. pylori strains examined. The vacuoles increase in number and size during extended incubation, resulting in vacuolar and cellular degeneration after 24 h to 48 h. Vacuolation of gastric epithelial cells is also observed in vivo during infection by H. pylori. A high molecular weight, heat labile protein is believed to be responsible for vacuolation and to significantly contribute to the development of gastroduodenal disease in humans. The mechanism by which the cytotoxin exerts its effect is unknown, as is the intracellular origin of the vacuolar membrane and contents. Acridine orange is a membrane-permeant weak base that initially accumulates in low-pH compartments. We have used acridine orange accumulation in conjunction with confocal laser scanning microscopy of toxin-treated cells to begin probing the nature and origin of these vacuoles.

Expression Analysis of Genes Involved in Peanut Seed Dormancy Release (Arachis hypogaeaL.)

2015 ◽  
Vol 41 (6) ◽  
pp. 845 ◽  
Author(s):  
Jing CHEN ◽  
Ling JIANG ◽  
Chun-Ming WANG ◽  
Xiao-Hui HU ◽  
Hu-Qu ZHAI ◽  
...  
Keyword(s):  
Seed Dormancy ◽  
Expression Analysis ◽  
Dormancy Release ◽  
Peanut Seed

scholarly journals Discovery of the freshwater sponge genus Corvospongilla Annandale (Porifera: Spongillida) in Australia with the description of a new species and phylogeographic implications

Limnology ◽  
2021 ◽  
Author(s):  
Renata Manconi ◽  
Dirk Erpenbeck ◽  
Jane Fromont ◽  
Gert Wörheide ◽  
Roberto Pronzato
Keyword(s):  
New Species ◽  
Morphological Traits ◽  
Freshwater Sponge ◽  
Freshwater Sponges ◽  
Molecular Analyses ◽  
Biogeographic Patterns ◽  
Hydrographic Basin ◽  
Integrative Systematics ◽  
North Western ◽  
A New Species

AbstractA recent discovery of freshwater sponges in an unexplored hydrographic basin in north-western Australia provided the opportunity to investigate the genus Corvospongilla Annandale (Spongillida: Spongillidae) using integrative systematics. Emendation of the genus diagnosis is provided. A comparative analysis of a Corvospongilla global dataset of morphological traits together with biogeographic patterns disclosed a new Australasian Corvospongilla species and along with molecular analyses provided the basis for a phylogenetic and phylogeographic tree for some Asian, Afrotropical and Australasian lineages.

scholarly journals A Turn-ON fluorometric biosensor based on ssDNA immobilized with a metal phenolic nanomaterial for the sequential detection of Pb(ii) and epirubicin cancer drug

RSC Advances ◽  
2021 ◽  
Vol 11 (20) ◽  
pp. 12361-12373
Author(s):  
A. Arunjegan ◽  
P. Rajaji ◽  
S. Sivanesan ◽  
P. Panneerselvam
Keyword(s):  
Acridine Orange ◽  
Cancer Drug ◽  
Sequential Detection ◽  
Label Free ◽  
Fluorescent Biosensor ◽  
Turn On

In this paper, we propose a fluorescent biosensor for the sequential detection of Pb2+ ions and the cancer drug epirubicin (Epn) using the interactions between label-free guanine-rich ssDNA (LFGr-ssDNA), acridine orange (AO), and a metal–phenolic nanomaterial.

Multimode binding and stimuli responsive displacement of acridine orange dye complexed with p-sulfonatocalix[4/6]arene macrocycles

2020 ◽  
Vol 22 (23) ◽  
pp. 13306-13319
Author(s):  
Mhejabeen Sayed ◽  
Dona M. Tom ◽  
Haridas Pal
Keyword(s):  
Acridine Orange ◽  
Dynamic Quenching ◽  
Stimuli Responsive ◽  
Turn On ◽  
Pictorial Presentation ◽  
Fluorescence Turn On ◽  
Multi Mode

Pictorial presentation of the different aspects as displayed by the AOH+–SCXn systems in regard to multi-mode binding, dynamic quenching and stimuli responsive fluorescence “turn ON”, demonstrating very rich supramolecular photochemistry.

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