Dormancy Release and Development from Gemmules of the Fresh-Water Sponge, Spongilla lacustris: A Supravital Study with Acridine Orange

1983 ◽  
Vol 102 (4) ◽  
pp. 309 ◽  
Author(s):  
Frederick W. Harrison ◽  
Ronald R. Cowden
Keyword(s):  
Acridine Orange ◽  
Fresh Water ◽  
Dormancy Release

Vacuoles Induced in Mammalian Cells by Helicobacter Cytotoxin are Acidic Organelles

1990 ◽  
Vol 48 (3) ◽  
pp. 168-169
Author(s):  
M. H. Chestnut ◽  
C. E. Catrenich
Keyword(s):  
Acridine Orange ◽  
Mammalian Cells ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ulcer Disease ◽  
Gastroduodenal Disease ◽  
H Pylori

Helicobacter pylori is a non-invasive, Gram-negative spiral bacterium first identified in 1983, and subsequently implicated in the pathogenesis of gastroduodenal disease including gastritis and peptic ulcer disease. Cytotoxic activity, manifested by intracytoplasmic vacuolation of mammalian cells in vitro, was identified in 55% of H. pylori strains examined. The vacuoles increase in number and size during extended incubation, resulting in vacuolar and cellular degeneration after 24 h to 48 h. Vacuolation of gastric epithelial cells is also observed in vivo during infection by H. pylori. A high molecular weight, heat labile protein is believed to be responsible for vacuolation and to significantly contribute to the development of gastroduodenal disease in humans. The mechanism by which the cytotoxin exerts its effect is unknown, as is the intracellular origin of the vacuolar membrane and contents. Acridine orange is a membrane-permeant weak base that initially accumulates in low-pH compartments. We have used acridine orange accumulation in conjunction with confocal laser scanning microscopy of toxin-treated cells to begin probing the nature and origin of these vacuoles.

Aplikasi metode tahanan jenis dalam studi geologi karst Gua Seropan di Gunung Kidul, Yogyakarta

2017 ◽  
Vol 12 (2) ◽  
pp. 105-116
Author(s):  
Pulung A. Pranantya ◽  
Nurlia Sadikin
Keyword(s):  
Fresh Water ◽  
Ground Surface ◽  
Water Source ◽  
Raw Water ◽  
Limited Information ◽  
Resistivity Method ◽  
Central Java ◽  
Underground River ◽  
Initial Exploration

In terms of geology, most areas in south of the Gunungkidul District in Central Java consist of the Wonosari formation limestone. The land is generally very dry and source of raw water is also difficult to reach. Findings on the existence of underground river in caves, however, indicate the potential amount of water within the area, especially in the eastern part of the Gunungkidul District. Although limited information available, some fishermen have discovered that Seropan cave contains fresh water source. This cave is situated at 65 m below the cliff. Initial exploration, which done using a multichannel resistivity method, confirmed the availability of freshwater in the cave and underground river. The isopach of cave depth is found in ranges of 80 200 m below the ground surface. The water of Seropan cave can be utilized by implementing pipeline or by drilling at the suggested point based on the interpretation results, i.e. 110o2223.6388 EL 8o42.874 SL. [DY1][PP2][DY1]Perbaiki grammarIn terms of geology, most areas in south of Gunungkidul District in Central Java consist of the Wonosari formation limestone. The land is generally very dry and source of raw water is also difficult to reach. Findings on the exixtence of underground river in caves, however, indicate potential amount of water within the area especially in eastern part of Gunungkidul District. Although limited information available, some fishermans has discovered that Seropan cave contain fresh water source. This cave is situated at 65 m below the cliff. Initial exploration, which done using multichannel resistivity method, confirmed the availability of freshwater in the cave and underground river. The iso pach of cave depth is found in ranges of 80 200 m below the ground surface. The water of Seropan cave can be utilized by implementing pipeline or by drilling at the suggested point based on the interpretation results i.e. 110o2223.6388 EL 8o42.874 SL.[PP2]Sudah diperbaiki

STUDY OF POND STATUS IN INDIA : A REVIEW

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
V.K. YADAV ◽  
SONAM SHARMA ◽  
A.K. SRIVASTAVA ◽  
P.K. KHARE
Keyword(s):  
Fresh Water ◽  
Land Surface ◽  
Human Population ◽  
Climate Changes ◽  
Hydrological Cycle ◽  
Habitat Destruction ◽  
Conservation Strategy ◽  
Goods And Services ◽  
Human Use ◽  
Over Exploitation

Ponds are an important fresh water critical ecosystem for plants and animals providing goods and services including food, fodder, fish, irrigation, hydrological cycle, shelter, medicine, culture, aesthetic and recreation. Ponds cover less than 2 percent of worlds land surface. Ponds are important source of fresh water for human use. These are threatened by urbanization, industrialization, over exploitation, fragmentation, habitat destruction, pollution, illegal capturing of land and climate changes. These above factors have been destroying ponds very rapidly putting them in danger of extinction of a great number of local biodiversity. It is necessary to formulate a correct conservation strategy for pond restoration in order to meet the growing needs of fresh water by increasing the human population. Some measures have been compiled and proposed in the present review.

EVALUATION OF PTYCHOBOTHRIDEAN CESTODES USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FROM FRESH WATER FISHES IN GODAVARI BASIN (M.S.) INDIA

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
SUNITA BORDE ◽  
ASAWARI FARTADE ◽  
AMOL THOSAR ◽  
RAHUL KHAWAL
Keyword(s):  
Fresh Water ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Band Pattern ◽  
Genomic Variation ◽  
Polymorphic Band ◽  
Rapd Profile ◽  
Band Size ◽  
Pcr Product ◽  
The Common

Ptychobothridean genera like Senga and Circumoncobothrium are the common parasites of fresh water fishes. The genotypic study of these parasites was taken by RAPD. The RAPD profile of these two parasites were not similar to each other as depicted by the band pattern in picture. These results suggest the presence of inter-specific polymorphism among cestode parasites of two different genera for RAPD analysis. The present study demonstrated that genetic differentiation of cestode parasites could be accomplished on the basis of genomic variation with polymorphic band pattern using RAPD. All the detected bands (PCR product) were polymorphic and band size ranged from 500-5000 bp in length. The RAPD of profiles using GBO-31, GBO-32, GBO-33, GBO-34, GBO-35 and GBO-36. Primers were able to characterize inter-specific polymorphism among the two genus ( Senga and Circumoncobothrium ). Genetic analysis suggests that Senga and Circumoncobothrium show genetic diversity with respect to RAPD patterns using all the six primers used for the present study. The genetic distance between the analyzed genuses ranged from 0.14 to 0.80. The differentiation of the two parasites on the basis of genetic markers could greatly facilitate study on the biology of these parasites.

DESCRIPTION OF CIRCUMONCOBOTHRIUM SINGHII N.SP. FROM CLARIAS BATRACUS OF SAVITRI RIVER AT DAPOLI DISTRICT RATNAGIRI (M.S.) INDIA

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
R.R. DANDAWATE
Keyword(s):  
Life Cycle ◽  
Fresh Water ◽  
Summer Season ◽  
Fresh Water Fish ◽  
Water Fish

Present paper deals with study of cestode parasites of genus Circumoncobothrium from fresh water fish Clarius batracus at Savitri river, (Dapoli) for the percentages of infection occurance during summer season,minimum during winter and tolarate during rainy season.The parasite mainly infected the intestine of host and fed on nutrients from digested food. It completed its life cycle in the intestine of host. By camparing different characters of it to identify that the species is new

PREVALENCE OF PSEUDOINVERTA ORAIENSIS IN FRESH WATER FISH, CLARIAS BATRACHUS

2019 ◽  
Vol 25 (1) ◽  
Author(s):  
ADITYA NARAYAN
Keyword(s):  
Fresh Water ◽  
Monsoon Season ◽  
Winter Season ◽  
Uttar Pradesh ◽  
Summer Season ◽  
Clarias Batrachus ◽  
Fresh Water Fish ◽  
Water Fish

The present investigation deals with the prevalence of infection of cestode, Pseudoinverta oraiensis19 parasitizing Clarias batrachus from Bundelkhand Region (U.P.) India. The studies were recorded from different sampling stations of Bundelkhand region of Uttar Pradesh. For this study 360 fresh water fish, Clarias batrachus were examined. The incidence of infection, monsoon season (17.50%) followed by winter season (20.00%) whereas high in summer season (30.00%).

Survey on encysted cercariae of trematodes from fresh-water fishes in Mangyeong riverside area

1983 ◽  
Vol 21 (2) ◽  
pp. 187 ◽  
Author(s):  
Jae Ku Rhee ◽  
Ho Il Lee ◽  
Byeong Kirl Baek ◽  
Pyung Gil Kim
Keyword(s):  
Fresh Water
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