scholarly journals Placental/Umbilical Cord Blood (PCB) Stem Cells for Transplantation: Early Clinical Outcomes and the Status of Ex Vivo Expansion Strategies.

2000 ◽  
Vol 49 (4) ◽  
pp. 141-151 ◽  
Author(s):  
Annette von Drygalski ◽  
John W. Adamson
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Clinical Outcomes ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
The Status ◽  
Early Clinical Outcomes ◽  
Expansion Strategies

Ex vivo Expansion of Umbilical Cord Blood Stem Cells Using Different Combinations of Cytokines and Stromal Cells

2007 ◽  
Vol 118 (3) ◽  
pp. 153-159 ◽  
Author(s):  
Manisha Madkaikar ◽  
Kanjaksha Ghosh ◽  
Maya Gupta ◽  
Suchitra Swaminathan ◽  
Dipika Mohanty
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Stromal Cells ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells

N-rasm-Transduced Fibroblasts Facilitate the Expansion and Engraftment of Umbilical Cord Blood Stem Cells.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3196-3196
Author(s):  
Alla Dolnikov ◽  
Tiffany Holmes ◽  
Sylvie Shen ◽  
Robert Knight ◽  
Tracey A. O’Brien
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Hematopoietic Progenitor ◽  
Soluble Factors ◽  
Cd34 Cells

Abstract Clinical trials using ex-vivo expanded umbilical cord blood (UCB) cells have thus far failed to demonstrate significant improvement in speed of hematopoietic recovery suggesting better understanding of self-renewal, proliferation, homing and engraftment capabilities of cord blood is needed. Recent progress has been made in the identification and understanding of the cellular niche for hematopoietic stem cells (HSCs) within bone marrow however attempts to identify soluble factors that regulate HSC engraftment to bone marrow have been less successful. We have demonstrated that N-rasm transduced NIH 3T3 fibroblasts release soluble factors that support the ex-vivo expansion of UCB CD34+ hematopoietic progenitor cells (HPCs). UCB CD34+ cells were cultured in combination with cytokines : TPO, SCF, IL-6 and flt3-ligand(all at 100ng/ml). Significant (7-fold) expansion of total nucleated cells was seen on day 19 in cultures complemented with medium produced by N-rasm -transduced fibroblasts (N-rasm medium), compared to control cells (GFP- transduced fibroblasts (GFP medium)). Maximum expansion of UCB CD34+ cells (2-fold) and the most primitive sub-population of CD34+ CD117+ cells (2-fold) were seen on day 4 in N-rasm medium cells compared to GFP medium cells. Furthermore, increased numbers of cycling cells were observed in UCB CD34+ cell cultures supplemented with N-rasm medium. Significant (3-fold) expansion of CD34+CXCR4+ cells enriched with the NOD/SCID mice engrafting cells, was also observed. This increased expansion of CD34+CXCR4+ cells correlated with a 3-fold increased engraftment of human UCB cells in a NOD/SCID mouse model confirming retention of engraftment capability of expanded cells. This data demonstrates that N-rasm -transduced fibroblasts secrete soluble factors that promote ex-vivo expansion and in-vivo engraftment of primitive human hematopoietic progenitor/stem cells derived from UCB. Proteins that are secreted by N-rasm -transduced fibroblasts that activate hematopoiesis are currently being identified. Our data demonstrates the potential of N-rasm-transduced fibroblasts as a reservoir for the discovery of novel secreted proteins that regulate ex-vivo expansion and engraftment of human UCB HSCs and may represent a potential novel expansion strategy allowing greater use UCB as a stem cell source for allogeneic transplantation.

Direct ex vivo expansion of hematopoietic stem cells from umbilical cord blood on membranes

2010 ◽  
Vol 351 (1-2) ◽  
pp. 104-111 ◽  
Author(s):  
Akon Higuchi ◽  
Siou-Ting Yang ◽  
Pei-Tsz Li ◽  
Miho Tamai ◽  
Yoh-ichi Tagawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Hematopoietic Stem
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