A STUDY TO ASSESS THE EFFECTIVENESS OF PLANNED TEACHING PROGRAMME REGARDING STEM CELLS AMONG ELIGIBLE COUPLES IN SELECTED AREAS OF AHMEDABAD

Introduction: Stem cells have tremendous promise to helping us to understand and treat a range of various diseases, injuries and other health related conditions. Their potential is evident in the use of cord blood stem cells to treat diseases of the blood, A cord blood stem cells therapy has saved the lives of thousands of children with leukemia and can be seen in the use of stem cells for tissue grafts to treat diseases or injury to the bone, skin and surface of the eye. Materials and Methods: This pre-experimental study was conducted on eligible couples with sample size of age between 15-45 years, willingness to participate in study in selected areas of Ahmedabad. The investigator adopted purposive technique to select sample. Data was collected using 4 demographic variables & 20 questionnaires regarding stem cells, its uses, diseases treated by stem cells & stem cell banking were included. A pre-test was conducted on 40 samples after which the planned teaching programme was implemented followed by the post test. Result: Data gathered was analyzed and interpreted using both experimental and inferential statistics. The mean and SD of pre-test was 5.725 and 1.3957, whereas the mean and SD of post- test was 16.25 and 1.1036, the calculated t value was greater than tabulated t value. Hence the null hypothesis was rejected and the research hypothesis was accepted. The result shows that when planned teaching program was given to eligible couples age group people, they achieve the best scores. Conclusion: This Study intends to assess the effectiveness on plan teaching program regarding stem cells among eligible couple in selected areas of Ahmedabad. This Study reveals that the post-test knowledge score is higher than the pre-test knowledge score regarding stem cells storage, stem cells banking among the eligible couple.

Umbilical cord blood stem cells transplantation in a patient with severe progressive supranuclear palsy: a case report

Background Progressive supranuclear palsy is a neurodegenerative condition that worsens over time. Given the lack of targeted treatments, patients with severe progressive supranuclear palsy have very low life expectancy. Case presentation We present a case of a 61-year-old Chinese man with severe progressive supranuclear palsy and treated with umbilical cord blood stem cells transplantation. After the umbilical cord blood stem cells therapy, his neurologic symptoms stopped deteriorating, his muscle rigidity was mildly improved, and he remains alive for more than 8 years. Conclusions Umbilical cord blood stem cells transplantation may be an alternative therapy for patients with severe progressive supranuclear palsy.

Clinical Practice of Umbilical Cord Blood Stem Cells in Transplantation and Regenerative Medicine - Prodigious Promise for Imminent Times

: The umbilical cord blood is usually disposed of as an unwanted material after parturition; however, today, it is viewed as a regenerative medication so as to create the organ tissues. This cord blood gathered from the umbilical cord is made up of mesenchymal stem cells, hematopoietic stem cells, and multipotent non-hematopoietic stem cells having many therapeutic effects as these stem cells are utilized to treat malignancies, hematological ailments, inborn metabolic problem, and immune deficiencies. Presently, numerous clinical applications for human umbilical cord blood inferred stem cells, as stem cell treatment initiate new research. These cells are showing such a boon to stem cell treatment; it is nevertheless characteristic that the prospect of conservation of umbilical cord blood is gaining impetus. Current research works have demonstrated that about 80 diseases, including cancer, can be treated or relieved utilizing umbilical cord blood stem cells, and every year, many transplants have been effectively done around the world. However, in terms of factors, including patient selection, cell preparation, dosing, and delivery process, the treatment procedure for therapy with minimally manipulated stem cells can be patented. It is also worth thinking about how this patent could affect cord blood banks. Meanwhile, the utilization of cord blood cells is controversial and adult-derived cells may not be as successful, so numerous clinicians have begun working with stem cells that are acquired from umbilical cord blood. This review epitomizes a change in outlook from what has been completed with umbilical cord blood cell research and cord blood banking on the grounds that cord blood cells do not require much in the method of handling for cryopreservation or for transplantation in regenerative medicine.

Effects of Umbilical Cord Blood Stem Cells on Coronary Artery Damage in Mice with Kawasaki Disease By High-Frequency Ultrasound

Objective: to use lactobacillus casein cell wall extract (LCWE) to induce Kawasaki disease (KD) mouse model, and analyse the process of coronary artery damage and effects of umbilical cord blood stem cells by high-frequency ultrasound in small animals. Methods: LCWE was prepared and 18 BALB/c pups were randomly divided into two groups: 15 in KD model group and 3 in the normal control group. KD model was induced by a single intraperitoneal injection of 0.5ml LCWE in the model group, and changes of the coronary artery were observed at 2d, 21d and 30d after the injection, respectively. From the 16th day of modeling, 300 μL PBS was injected intraperitoneally daily in the control group and model group. The hUC-MSCs 300 μL (105/mL) were intraperitoneally injected daily for 10 consecutive days in the stem cell group. The mice were sacrificed in batches on day 2, 15, 21 and 30, and the morphological changes of coronary arteries were observed by echocardiography and histopathology. Results: the change of coronary artery diameter could be accurately measured by high-frequency small animal ultrasound. At 21d, the coronary arteries of the model group were widened compared with those of the control group. At 30d, there was no significant difference between the model group and the previous model group. Histopathology showed slight swelling of the epicardium of aortic valve, mitral valve, right ventricle and atrium, scattered infiltration of a few neutrophils, dilatation of the coronary artery lumen, necrosis and disintegration of a small number of myocardial cells, and significant hyperplasia of local fibrous connective tissue accompanied by solid calcium salt deposits. After hUC-MSCs intervention treatment, B-ultrasound showed a decrease in the main coronary artery diameter, histopathology showed multiple lymphocytes, eosinophilic granulocytes, and mononuclear cell infiltration in the left atrial appendage of mice, and no obvious vascular inflammatory reaction or other obvious abnormalities were observed. Conclusion: high-frequency ultrasound can be used to clearly obtain the coronary artery image of KD mice and dynamically observe the evolution process of coronary artery diameter, which provides more diagnostic basis for the treatment of clinical KD. And hUC-MSCs intervention reduced the pathological lesion of coronary artery inflammation in mice compared with the model group.