scholarly journals Effect of Stemregenin1 and SB431542 Small Molecules on Ex Vivo Expansion of Umbilical Cord Blood Hematopoietic Stem Cells on Biocompatible Polyethersulfone Nanofiber Scaffolds

2018 ◽  
Author(s):  
Sorush Niknamian
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Small Molecules ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Hematopoietic Stem ◽  
Nanofiber Scaffolds

Direct ex vivo expansion of hematopoietic stem cells from umbilical cord blood on membranes

2010 ◽  
Vol 351 (1-2) ◽  
pp. 104-111 ◽  
Author(s):  
Akon Higuchi ◽  
Siou-Ting Yang ◽  
Pei-Tsz Li ◽  
Miho Tamai ◽  
Yoh-ichi Tagawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Hematopoietic Stem

scholarly journals Effect of Stemregenin1 and SB431542 small molecules on ex vivo expansion of umbilical cord blood hematopoietic stem cells on biocompatible polyethersulfone nanofiber scaffolds

2019 ◽  
Author(s):  
Sorush Niknamian
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Small Molecules ◽  
Umbilical Cord ◽  
Colony Formation ◽  
Ex Vivo ◽  
Medical Application ◽  
Ex Vivo Expansion ◽  
Hematopoietic Stem

Cord blood Hematopoietic stem cells (HSCs) with several advantages including low chance of viral contamination and low rate of Graft versus host disease (GVHD) are appropriate candidate for vast medical applications such as transplantation. The main obstacle of cord blood HSCs is the low number cells. To improve ex-vivo expansion of umbilical cord HSCs we introduced a new culture system. Isolated HSCs were seeded in Three-dimensional(3D) on Polyethersulfone(PES) scaffolds and Two-dimensional(2D) culture conditions and treated with SB431542 and Stemregenin1(SR1) small molecules. On the fifth and tenth days the expanded cells in different groups were investigated for expression of specific markers by flow cytometry, expression of some stemness genes by qRT-PCR and colony formation by methocult medium. SR1 molecule significantly increased expansion of CD34+ cells while SB431542 induced more CD34+/38+ cells. Also SB431542 treated cells showed higher colony formation capacity. SR1 increased the expression of c-Myc, HOXB4 and SALL4 while SB431542 seemed to inhibit HOXB4 expression and increase SALL4.Together all, this study introduced a new ex vivo culture setting for further medical application of HSCs. Our data showed simultaneous use of these two small molecules can provide appropriate outcome for HSCs transplantation includes both of engraftment and repopulation.

scholarly journals Isolation and <i> Ex Vivo</i> Expansion of Human Hematopoietic Stem Cells Derived from Umbilical Cord Blood

2021 ◽  
Vol 11 (01) ◽  
pp. 1-13
Author(s):  
Mao Pang ◽  
Shiqian Han ◽  
Meng Ren ◽  
Dongwei Guan ◽  
Tianying Kuang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Ex Vivo ◽  
Hematopoietic Stem

scholarly journals Combination of SB431542, Chir9901, and Bpv as a Novel Supplement in the Culture of Umbilical Cord Blood Hematopoietic Stem Cells

2020 ◽  
Author(s):  
Elaheh Afzal ◽  
Morteza Zarrabi ◽  
Mohammad Hassan Asghari ◽  
Marzieh Ebrahimi
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Small Molecules ◽  
Small Molecule ◽  
Umbilical Cord Blood ◽  
Ex Vivo ◽  
Self Renewal ◽  
Hematopoietic Stem ◽  
Clonogenic Potential

Abstract Background: Small molecule compounds have been well recognized for their promising power in generation, expansion and maintenance of embryonic or adult stem cells. The aim of this study was to identify a novel combination of small molecules in order to optimize the ex vivo expansion of umbilical cord blood derived-CD34+ cells. Methods: Considering the most important signaling pathways involved in the self-renewal of hematopoietic stem cells, CB-CD34+ cells were expanded with cytokines in the presence of seven small molecules including SB, PD, Chir, Bpv, Pur, Pμ and NAM. Eliminativism approach was used to find the best combination of selected small molecules for effective ex vivo expansion of CD34+ cell. In each step, proliferation, self-renewal, and clonogenic potential of the expanded cells as well as expression of some hematopoietic stem cell related genes were studied. Finally, the engraftment potential of expanded cells was also examined by the mouse intra-uterine transplantation model. Results: Our data shows that simultaneous use of SB431542 (TGF-β inhibitor), Chir9901 (GSK3 inhibitor) and Bpv (PTEN inhibitor), resulted in a 50-fold increase in the number of CD34+CD38- cells. This was further reflected in approximately 3 times increase in clonogenic potential of the small molecule cocktail-expanded cells. These cells, also, showed a 1.5-fold higher engraftment potential in the peripheral blood of NMRI model of in utero transplantation. These results are in total conformity with up-regulation of HOXB4, GATA2 and CD34 marker gene as well as CXCR4 homing gene. Conclusion: Taken together, our findings introduce a novel combination of small molecules to improve the yield of existing protocols used in the expansion of hematopoietic stem cells.

scholarly journals Combination of SB431542, Chir9901, and Bpv as a novel supplement in the culture of umbilical cord blood hematopoietic stem cells

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Morteza Zarrabi ◽  
Elaheh Afzal ◽  
Mohammad Hassan Asghari ◽  
Marzieh Ebrahimi
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Small Molecules ◽  
Small Molecule ◽  
Umbilical Cord Blood ◽  
Ex Vivo ◽  
Self Renewal ◽  
Hematopoietic Stem ◽  
Clonogenic Potential

Abstract Background Small molecule compounds have been well recognized for their promising power in the generation, expansion, and maintenance of embryonic or adult stem cells. The aim of this study was to identify a novel combination of small molecules in order to optimize the ex vivo expansion of umbilical cord blood-derived CD34+ cells. Methods Considering the most important signaling pathways involved in the self-renewal of hematopoietic stem cells, CB-CD34+ cells were expanded with cytokines in the presence of seven small molecules including SB, PD, Chir, Bpv, Pur, Pμ, and NAM. The eliminativism approach was used to find the best combination of selected small molecules for effective ex vivo expansion of CD34+ cell. In each step, proliferation, self-renewal, and clonogenic potential of the expanded cells as well as expression of some hematopoietic stem cell-related genes were studied. Finally, the engraftment potential of expanded cells was also examined by the mouse intra-uterine transplantation model. Results Our data shows that the simultaneous use of SB431542 (TGF-β inhibitor), Chir9901 (GSK3 inhibitor), and Bpv (PTEN inhibitor) resulted in a 50-fold increase in the number of CD34+CD38− cells. This was further reflected in approximately 3 times the increase in the clonogenic potential of the small molecule cocktail-expanded cells. These cells, also, showed a 1.5-fold higher engraftment potential in the peripheral blood of the NMRI model of in utero transplantation. These results are in total conformity with the upregulation of HOXB4, GATA2, and CD34 marker gene as well as the CXCR4 homing gene. Conclusion Taken together, our findings introduce a novel combination of small molecules to improve the yield of existing protocols used in the expansion of hematopoietic stem cells.

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