scholarly journals Influence of the elevated ambient temperature on immunoglobulin G and immunoglobulin G subclasses in sera of Wistar rats

2003 ◽  
Vol 60 (6) ◽  
pp. 657-661 ◽  
Author(s):  
Maja Jurhar-Pavlova ◽  
Aleksandar Petlichkovski ◽  
Dejan Trajkov ◽  
Olivija Efinska-Mladenovska ◽  
Todor Arsov ◽  
...  
Keyword(s):  
Immune System ◽  
Ambient Temperature ◽  
Immunoglobulin G ◽  
Wistar Rats ◽  
Control Group ◽  
Immunoglobulin G Subclasses ◽  
Male Wistar Rats ◽  
Regulation Mechanisms ◽  
Experimental Group ◽  
Igg1 Level

The aim of our research was to examine changes in the immune system of the rats influenced by the elevated ambient temperature. Male Wistar rats were divided, into 2 groups and housed at 20 ? 2?C (n=64, control group) and 35 ? 1?C (n=74, experimental group), during precise timing of 1, 4, 7, 14, 21, and 30 days. All the animals were given food and water ad libitum, and were lighted during 12 hours per day. We have measured IgG, IgG1, IgG2a, IgG2b and IgG2c. The obtained results showed significant elevation in the level of IgG after 4 and 7 days (+32%), IgG2a after 7th (+88%), 14th and 21nd day (+110%), IgG2b after 14 days (+60%) at 35 ? 1?C compared with the control group at 20 ? 2?C. IgG1 level was not affected and IgG2c showed significant decrease after 21st day at 35 ? 1?C. In conclusion, during the elevated ambient temperature the immune system is activated as one of the regulation mechanisms in homeostasis and survival of the population.

Effect of Strychnine Sulfate on Locomotor Activity

1965 ◽  
Vol 20 (3_suppl) ◽  
pp. 1049-1053 ◽  
Author(s):  
Robert G. Gibby ◽  
Robert G. Gibby ◽  
George B. Kish ◽  
George C. Theologus
Keyword(s):  
Locomotor Activity ◽  
Open Field ◽  
Exploratory Behavior ◽  
Normal Saline ◽  
Behavioral Change ◽  
Wistar Rats ◽  
Spontaneous Locomotor Activity ◽  
Control Group ◽  
Male Wistar Rats ◽  
Experimental Group

The effect of strychnine sulfate upon spontaneous locomotor activity in an open field was determined for 20 naive male Wistar rats. The experimental Ss were injected ip with 1.0 cc/kg of a 01% solution of strychnine sulfate in normal saline and the control Ss received a similar injection of 1.0 cc/kg of normal saline. All Ss were given 2 trials 7 days apart in the open field, with the injections preceding the second trial. The behavioral change from Trial 1 to Trial 2 for the control group was compared with that for the experimental group. Strychnine sulfate significantly reduced spontaneous locomotor activity in rats. A significant correlation was found between scores on the first and second trials of the experimental Ss. It was suggested that the concept of “orienting-exploratory behavior” could account for reduced locomotor activity.

scholarly journals Changes of the Biochemical Parameters of the Blood Serum of Wistar rats Exposed to Chronic Stres

2019 ◽  
pp. 62-67
Author(s):  
K. A. Kidun ◽  
A. N. Litvinenko ◽  
T. S. Ugolnik ◽  
N. M. Golubykh ◽  
E. K. Solodova
Keyword(s):  
Blood Serum ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Mature Male ◽  
Control Group ◽  
Physiological Processes ◽  
Male Wistar Rats ◽  
Increased Activity ◽  
Sexually Mature ◽  
Experimental Group

Objective: to assess the effect of chronic nonspecific stress on changes of the biochemical parameters of the blood serum in male Wistar rats.Material and methods. The experimental study was performed on sexually mature male Wistar rats. The experimental group (n = 71) was exposed to chronic nonspecific stress according to the Ortiz method. The control group of the animals included intact animals (n = 31).Results. The study has revealed changes in the biochemical composition of the blood serum of the rats: increased activity of ALT, LDH, ALP, urea, uric acid, and cholesterol, a decreased level of triglycerides.Conclusion. These changes may indicate the alteration of the level of the intensity of the physiological processes of energy supply in the conditions of chronic stress.

scholarly journals Effect of glutamine ingestion on the progression of induced periodontitis: experimental study in rats

2018 ◽  
Vol 47 (2) ◽  
pp. 119-123
Author(s):  
Aurélio Rosa da SILVA JUNIOR ◽  
Alex SEMENOFF SEGUNDO ◽  
Tereza Aparecida Delle Vedove SEMENOFF ◽  
Natalino Francisco da SILVA ◽  
Cervantes CAPOROSSI
Keyword(s):  
Experimental Study ◽  
Periodontal Disease ◽  
Wistar Rats ◽  
Saline Solution ◽  
Glutamine Supplementation ◽  
Control Group ◽  
Defense System ◽  
Male Wistar Rats ◽  
Experimental Group ◽  
System Objective

Abstract Introduction With glutamine supplementation there is better performance in the body’s defense system. Objective The aim of this study was to analyze the effect of glutamine supplementation on ligand-induced periodontal disease in rats. Material and method 48 selected male Wistar rats were divided into 4 groups (N = 12): control group (CG) including healthy animals receiving daily saline solution via gavage; glutamine group (GG) including healthy animals receiving oral glutamine supplementation by gavage at a dose of 1.5 g / kg / day; periodontitis group (PG) including animals with induced periodontal disease on both upper sides receiving daily saline solution via gavage; experimental group (EG) including animals with induced periodontal disease on both upper sides receiving daily glutamine via gavage at a dose of 1.5 g / kg / day. On the 30th day, all animals were euthanized by excess anesthetic. Result In the various analyses, the EG (116.63 ± 22.50 mm2) compared to the CG (82.32 ± 7.48 mm2) obtained a p<0.05. The PG (143.15 ± 35.24 mm2) compared to the EG group (116.63 ± 22.50 mm2 ) presented a p<0.05. The PG (143.15 ± 35.24 mm2) compared to the CG (82.32 ± 7.48 mm2) presented a p<0.05. The GG (118.09 ± 10.4 mm2) compared to the CG (82.32 ± 7.48 mm2) presented a p>0.05. Conclusion Glutamine supplementation associated with induced periodontal disease demonstrated a smaller amount of bone loss compared to the periodontitis group.

scholarly journals Gestational exposure to the cannabinoid WIN 55,212-2 and its effect on the innate intestinal immune response

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Rosalía Hernández-Cervantes ◽  
Armando Pérez-Torres ◽  
Óscar Prospéro-García ◽  
Jorge Morales Montor
Keyword(s):  
Immune System ◽  
Wistar Rats ◽  
Trichinella Spiralis ◽  
Intestinal Parasites ◽  
Control Group ◽  
Prenatally Exposed ◽  
Mucus Production ◽  
Gestational Exposure ◽  
Male Wistar Rats ◽  
Marijuana Consumption

AbstractThe consequences of marijuana consumption during pregnancy and its effects on the function of the immune system have been little studied. Marijuana is one of the most consumed recreational drugs among pregnant women, and it is known that gestational exposure to marijuana can have serious effects on the offspring after birth. In this study, we challenged the immune system of Wistar rats by infecting them with the parasitic nematode Trichinella spiralis. A treatment group of these animals was prenatally exposed to the cannabinoid WIN 55,212-2; a control group was not exposed. At 5 days of infection, the treated animals were less effective in eliminating intestinal parasites; moreover, this effect was correlated with a deficiency in mucus production, lower recruitment of eosinophils in the duodenum, and a reduced percentage of Tγδ and NK cells. In conclusion, the gestational administration of the synthetic cannabinoid WIN 55,212-2 induces lasting changes to the function of the immune system against infection with T. spiralis in male Wistar rats, making them more susceptible to infection.

scholarly journals PENGARUH LYSERGIC ACID DIETHYLAMIDE YANG TERDAPAT PADA LEM EHA-BOND TERHADAP KUALITAS SPERMATOZOA WISTAR JANTAN (Rattus norvegicus)

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Alfred Budiono ◽  
Benny Wantouw ◽  
Lusiana Satiawati
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Lysergic Acid ◽  
Raw Material ◽  
Lysergic Acid Diethylamide ◽  
Control Group ◽  
Spermatozoa Motility ◽  
Randomized Design ◽  
Male Wistar Rats ◽  
Experimental Group

Abstract: Lysergic Acid Diethylamide (LSD) is a psychotropic drug that can be found in glue. This substance acts as serotonine and dopamine agonists and results in hallucinogenic effect. LSD is a main raw material in glue and commonly available in an affordable price. Moreover, LSD also has a direct impact on spermatozoa motility and morphology. This study aimed to obtain the change in the spermatozoa quality of wistar rats exposed to Eha-Bond glue. This was an experimental study with a completely randomized design. Saples were 10 male wistar rats (Rattus norvegicus) divided into 2 groups: control group given just food and drink; and the experimental group exposed to Eha-Bond glue 1 hour per day for 52 days. The experimental group showed a decrease in motility by 61% of B category whilst no A category found compared to the control group that had both. Morphologically, the researcher discover 68% of the abnormal morphology and 32% of the normal morphology in the experimental group. Conclusion: LSD in Eha-Bond glue influenced the decrease in spermatozoa quality -the motility and morphology- but not the concentration of spermatozoa.Keywords: LSD, psychotropic, spermatozoa qualityAbstrak: Lysergic Acid Diethylamide (LSD) merupakan salah satu narkoba jenis psikotropika yang bekerja sebagai agonis serotonin dan dopamin serta menimbulkan efek halusinasi. LSD mudah didapat karena merupakan bahan baku pembuatan lem kayu, dijual bebas dalam bentuk lem, dan memiliki harga yang relatif terjangkau. LSD juga berdampak langsung terhadap motilitas dan morfologi spermatozoa. Penelitian ini bertujuan untuk mendapatkan perubahan kualitas spermatozoa setelah diberi paparan lem Eha-Bond. Penelitian yang menggunakan metode eksperimental dengan rancangan acak lengkap. Sepuluh ekor wistar jantan (Rattus norvegicus) dibagi dalam 2 kelompok yaitu kelompok kontrol yang hanya diberi makan dan minum dan kelompok perlakuan yang diberi paparan lem Eha-Bond 1jam per hari selama 52 hari. Kelompok perlakuan menunjukkan adanya penurunan motilitas ditandai dengan tidak ditemukannya motilitas kategori A sedangkan motilitas kategori B mencapai 61% dibandingkan dengan kelompok kontrol yang memiliki keduanya. Secara morfologi, hanya ditemukan sedikit morfologi spermatozoa normal pada kelompok perlakuan yaitu 32% sedangkan morfologi abnormal mencapai 68%. Simpulan: LSD dalam lem Eha-Bond berpengaruh menurunkan motilitas dan morfologi tetapi tidak berpengaruh pada konsentrasi spermatozoa.Kata kunci: LSD, psikotropika, kualitas spermatozoa

Pengaruh Profilaksis Ekstrak Ubi Jalar Ungu (Ipomoea batatas L.) terhadap Kadar Malondialdehida Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Karagenan

2018 ◽  
Vol 15 (2) ◽  
pp. 146
Author(s):  
BRILIAN DINANTI ◽  
FITRI HANDAJANI
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Wistar Rats ◽  
Group Method ◽  
Control Group ◽  
Intraplantar Injection ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Potato Extract ◽  
Purple Sweet Potato

<p>Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased  liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.</p><p>This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.</p><p>The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (<em>x̅</em>= 207,50) and group of rats induced by carrageenan (<em>x̅</em>=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (<em>x̅</em>= 232,50).</p><p>The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants.</p><p> </p><strong>Keywords: </strong>Liver, <em>Ipomoea batatas</em> L., Malondialdehyde, Anthocyanins

scholarly journals Cannabis Extract Has a Positive–Immunostimulating Effect through Proteolytic System and Metabolic Compounds of Honey Bee (Apis Mellifera) Workers

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2190
Author(s):  
Patrycja Skowronek ◽  
Łukasz Wójcik ◽  
Aneta Strachecka
Keyword(s):  
Immune System ◽  
Apis Mellifera ◽  
Protease Inhibitor ◽  
Control Group ◽  
Proteolytic System ◽  
Sugar Syrup ◽  
Administration Method ◽  
Cannabis Extract ◽  
Compound Concentration ◽  
Experimental Group

In the study, we assessed the effect of hemp extract on activities of resistance parameters and the metabolic compound concentration in adult workers’ hemolymph. Bees were divided into the following groups: (1) control group fed with mixture of sugar and water-glycerine solution, (2) experimental group with pure sugar syrup and inside with cotton strips soaked with hemp extract, (3) experimental group with a mixture of sugar syrup with hemp extract. Hemp extracts caused an increase in the protein concentrations and reduced the protease activities regardless of the administration method. The protease inhibitor activities were decreased only in the group that received hemp extract on the strips. The biomarker activities (ALP, ALT, AST) increased from the control group and workers feeding extract in syrup and decreased in workers supplemented with the extract on strips. In young, 2-day-old workers, the glucose concentration was higher in the groups feeding with the extract than in the control. Hemp extract influenced an increase in urea concentrations in workers’ hemolymph in comparison with the control. The hemp supplementation positively influences the immune system of workers, and the appropriate method of administration may be adapted to the health problems of bees.

scholarly journals Effects of Long-Term Supplementation of Bovine Colostrum on the Immune System in Young Female Basketball Players. Randomized Trial

Nutrients ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 118
Author(s):  
Anna Skarpańska-Stejnborn ◽  
Mirosława Cieślicka ◽  
Hanna Dziewiecka ◽  
Sławomir Kujawski ◽  
Anita Marcinkiewicz ◽  
...  
Keyword(s):  
Immune System ◽  
Physical Exercise ◽  
Exercise Program ◽  
Bovine Colostrum ◽  
Control Group ◽  
System Function ◽  
Basketball Players ◽  
Immune System Function ◽  
Experimental Group

An intensive physical exercise program could lead to a decrease in immune system function. Effects of long-term supplementation of bovine colostrum on the response of immune function on physical exercise test in athletes were examined. Twenty-seven elite female basketball players (age 16–19) were randomly assigned to either an experimental group or a control group. Eventually, n = 11 athletes completed intervention in the experimental group (3.2 g bovine colostrum orally twice a day for 24 weeks), while n = 9 athletes in the control group were given a placebo. Before the supplementation, after 3 and 6 months, subjects performed the physical exercise stress test. Before, just after, and 3 h after physical exercise testing, blood was drawn and immune system indicators were examined. Plasma interleukin (IL)-1alpha, IL-2, IL-10, IL-13, tumor necrosis factor (TNF) alpha, creatine kinase (CK MM), immunoglobulin G (IgG), insulin-like growth factor 1 (IGF1), and WBC, lymphocyte (LYM), monocyte (MON), and granulocyte (GRA) were measured. A statistically significant change in IL-10 in response to the exercise program during the supplementation period in both groups was observed (p = 0.01). However, the results of the rest of the comparisons were statistically insignificant (p > 0.05). Contrary to our initial hypothesis, there were no significant effects of bovine supplementation on the dynamics of immune system function indicators.

scholarly journals Impact of Vanadium Complexes Treatment on the Oxidative Stress Factors in Wistar Rats Plasma

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
R. Francik ◽  
M. Krośniak ◽  
M. Barlik ◽  
A. Kudła ◽  
R. Gryboś ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Reduced Glutathione ◽  
The Body ◽  
Stress Factors ◽  
Control Group ◽  
Vanadium Complexes ◽  
Healthy Male ◽  
Reducing Ability ◽  
Male Wistar Rats ◽  
Antioxidant Parameters

The aim of this study was to investigate the clinical efficacy of vanadium complexes on triglycerides (TG), total cholesterol (Chol), uric acid (UA), urea (U), and antioxidant parameters: nonenzymatic (FRAP—ferric reducing ability of plasma, and reduced glutathione—GSH) and enzymatic (glutathione peroxidase—GPx, catalase—CAT, and GPx/CAT ratio) activity in the plasma of healthy male Wistar rats. Three vanadium complexes: [VO(bpy)2]SO4⋅2H2O, [VO(4,4′Me2bpy)2]SO4⋅2H2O, and Na[VO(O2)2(bpy)]⋅8H2O are administered by gavage during 5 weeks in two different diets such as control (C) and high fatty (F) diets. Changes of biochemical and antioxidants parameters are measured in plasma. All three vanadium complexes statistically decrease the body mass growth in comparison to the control and fatty diet. In plasma GSH was statistically increased in all vanadium complexes-treated rats from control and fatty group in comparison to only control group. Calculated GPX/CAT ratio was the highest in the control group in comparison to others.

scholarly journals Anticarcinogenic effect of probiotic fermented milk and chlorophyllin on aflatoxin-B1-induced liver carcinogenesis in rats

2011 ◽  
Vol 107 (7) ◽  
pp. 1006-1016 ◽  
Author(s):  
M. Kumar ◽  
V. Verma ◽  
R. Nagpal ◽  
A. Kumar ◽  
P. V. Behare ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Lactobacillus Rhamnosus ◽  
Thiobarbituric Acid ◽  
Fermented Milk ◽  
Hepatoprotective Effect ◽  
Control Group ◽  
Time Interval ◽  
Group I ◽  
Male Wistar Rats ◽  
Aflatoxin B

The present investigation was carried out to evaluate the hepatoprotective effect of probiotic fermented milk (FM) containing Lactobacillus rhamnosus GG and Lactobacillus casei strain Shirota, alone as well as in combination with chlorophyllin (CHL) as an antioxidant agent in male Wistar rats administered aflatoxin-B1 (AFB1). AFB1 was injected intraperitoneally at the rate of 450 μg/kg body weight per animal twice a week for 6 weeks, maintaining an equal time interval between the two consecutive AFB1 administrations. A total of 125 male Wistar rats were randomly allocated to five groups, each group having twenty-five animals. Group I was offered FM containing L. rhamnosus GG and L. casei strain Shirota. Group II was administered AFB1 and served as the control group; group III was administered FM-AFB1, in which besides administering AFB1, FM was also offered. Group IV was offered CHL and AFB1, and group V was offered both FM and CHL along with AFB1. The rats were euthanised at the 15th and 25th week of the experiment and examined for the biochemical and hepatopathological profile. A significant reduction in thiobarbituric acid-reactive substances (TBARS) was observed in the FM–CHL–AFB1 group compared with the AFB1 control group. FM alone or in combination with CHL was found to show a significant (P < 0·05) hepatoprotective effect by lowering the levels of TBARS and by enhancing the activities of antioxidant enzymes such as glutathione peroxidase, superoxide dismutase, catalase and glutathione-S-transferase, indicating that probiotic FM alone or in combination with CHL possesses a potent protective effect against AFB1-induced hepatic damage.

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