scholarly journals Impact of Vanadium Complexes Treatment on the Oxidative Stress Factors in Wistar Rats Plasma

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
R. Francik ◽  
M. Krośniak ◽  
M. Barlik ◽  
A. Kudła ◽  
R. Gryboś ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Reduced Glutathione ◽  
The Body ◽  
Stress Factors ◽  
Control Group ◽  
Vanadium Complexes ◽  
Healthy Male ◽  
Reducing Ability ◽  
Male Wistar Rats ◽  
Antioxidant Parameters

The aim of this study was to investigate the clinical efficacy of vanadium complexes on triglycerides (TG), total cholesterol (Chol), uric acid (UA), urea (U), and antioxidant parameters: nonenzymatic (FRAP—ferric reducing ability of plasma, and reduced glutathione—GSH) and enzymatic (glutathione peroxidase—GPx, catalase—CAT, and GPx/CAT ratio) activity in the plasma of healthy male Wistar rats. Three vanadium complexes: [VO(bpy)2]SO4⋅2H2O, [VO(4,4′Me2bpy)2]SO4⋅2H2O, and Na[VO(O2)2(bpy)]⋅8H2O are administered by gavage during 5 weeks in two different diets such as control (C) and high fatty (F) diets. Changes of biochemical and antioxidants parameters are measured in plasma. All three vanadium complexes statistically decrease the body mass growth in comparison to the control and fatty diet. In plasma GSH was statistically increased in all vanadium complexes-treated rats from control and fatty group in comparison to only control group. Calculated GPX/CAT ratio was the highest in the control group in comparison to others.

Prevention and therapy of 1,2-dimethyl hydrazine induced colon carcinogenesis by Ferula assafoetida hydroalcoholic extract / 1,2-dimetilhidrazin ile indüklenmiş kalın bağırsak kanserlerinin Ferula assa-foetida ekstraktı kullanılarak önlenmesi ve tedavisi

2015 ◽  
Vol 40 (5) ◽  
Author(s):  
Fatemeh Torabi ◽  
Abolfazl Dadkhah ◽  
Faezeh Fatemi ◽  
Salome Dini ◽  
Mohsen Taghizadeh ◽  
...  
Keyword(s):  
Colon Carcinogenesis ◽  
Negative Control Group ◽  
Negative Control ◽  
Detoxification Enzymes ◽  
Control Group ◽  
Hydroalcoholic Extract ◽  
Reducing Ability ◽  
Male Wistar Rats ◽  
Antioxidant Parameters ◽  
Ferula Assafoetida

AbstractObjective: In this study, we have evaluated the chemopreventive and chemotherapeutic effects of Ferula assa-foetida hydroalcoholic extract in 1, 2-dimethyl hydrazine (DMH) induced colon carcinogenesis.Methods: Male Wistar rats were divided into 6 groups: a negative control group without DMH; control group with injected DMH (20 mg/Kg b.w) and four groups receiving DMH +F. assa- foetida extract (6.25 and 12.5 mg/Kg b.w) as chemopreventive and chemotherapeutic groups. The effects of the extracts were assessed by estimating the hepatic oxidative stress/antioxidant parameters such as malondialdehyde, glutathione and ferric reducing ability of plasma (MDA, GSH, FRAP) and the detoxification enzymes; glutathione S-transferase and Cytochrome PResults: The results showed that the F. assa-foetida extracts markedly reversed the increased levels of CYPConclusion: The achieved results suggested the beneficial effect of the extracts on DMH metabolic processes in the colon indicating its chemopreventive and chemotherapeutic effects on colon carcinogenesis induced by DMH.

scholarly journals Biochemical and Histopathological Studies of Key Tissues in Healthy Male Wistar Rats Fed on African Yam Bean Seed and Tuber Meals

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Omena Bernard Ojuederie ◽  
John Adebayo Ajiboye ◽  
Olubukola Oluranti Babalola
Keyword(s):  
Wistar Rats ◽  
Seed Meal ◽  
Control Group ◽  
Human Consumption ◽  
Healthy Male ◽  
Ether Anesthesia ◽  
African Yam Bean ◽  
Group I ◽  
Male Wistar Rats ◽  
Histopathological Studies

Food insecurity and malnutrition are currently major issues affecting most developing countries, especially on the African continent. To mitigate this effect, focus is being given to orphan or underutilized crops with immense potentials to boost food and nutrition security in Africa, such as the African yam bean (AYB) Sphenostylis stenocarpa. The effect of AYB seed and tuber meals on the tissues of the kidney, liver, and testis of healthy male Wistar rats were investigated in this study. Four accessions of AYB were used for this study, TSs 107, TSs 140, AYB 45, and AYB 57. Thirty rats were randomly assigned into five groups (n = 6). Group I was fed on standard pelletized rat chow (control), Group II fed on 50% seed meal, Group III fed on 100% seed meal, Group IV fed on 50% tuber meal, and Group-V fed on 100% tuber meal. At the end of the treatments, the animals were sacrificed after 72 h under light ether anesthesia, and biochemical and histopathological analyses were conducted on the tissues. Phytate concentration was higher in the seeds (TSs140 (550 mg 100g−1), AYB45 (460 mg 100g−1), and AYB57 (485 mg 100g−1)) compared to the tubers (TSs140 (14.8 mg 100g−1), AYB 45 (275 mg 100g−1), and AYB57 (240 mg 100g−1)). The consumption of 100% unprocessed AYB seeds caused liver and kidney damage in rats due to increased levels of aspartate aminotransferase (5.04 ± 1.62 U L−I), alanine aminotransferase (8.46 ± 2.43 U L−I), and lipid peroxidation (0.27 ± 0.02-unit mg−1protein). AYB tubers were innocuous to Wistar rats investigated. Good processing of AYB seeds is required for safe consumption by humans and livestock. This study has shown that tubers of AYB are safe for human consumption and should be utilized in meals as it contains fewer antinutrients and had no significant effect on the tissues examined in Wistar rats.

scholarly journals Strong Twirling-Rotating Manual Acupuncture with 4 r/s Is Superior to 2 r/s in Relieving Pain by Activating C-Fibers in Rat Models of CFA-Induced Pain

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Simin Song ◽  
Yuan Xu ◽  
Jiang Liu ◽  
Yadi Jia ◽  
Xiaowei Lin ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Control Group ◽  
Healthy Male ◽  
Intraplantar Injection ◽  
Pain Model ◽  
Analgesic Effects ◽  
C Fibers ◽  
C Fiber ◽  
Male Wistar Rats ◽  
Group 2

Background. Manual acupuncture (MA) with different stimulus frequencies may give rise to varying acupuncture effects. However, the intensity-effect relationship and the underlying mechanisms of MA remain unclear. Objective. To compare the analgesic effects of different frequencies of twirling-rotating MA on rats with complete Freund’s adjuvant- (CFA-) induced pain and explore the underlying mechanism via peripheral sensory nerves. Methods. First, 36 healthy male Wistar rats were randomly divided into 6 groups: control group, 2 r/s MA group (twirling-rotating MA with the frequency of 2 revolutions per second), 4 r/s MA group (twirling-rotating MA with the frequency of 4 revolutions per second), CFA group, CFA + 2 r/s MA group, and CFA + 4 r/s MA group. Rats in three CFA groups received an intraplantar injection of CFA to establish a pain model, while the rats in other three groups received an intraplantar injection of saline. Rats in the 2 r/s MA group and 4 r/s MA group were treated with the corresponding frequencies of twirling-rotating MA on bilateral Zusanli (ST36) and Kunlun (BL60) for 7 days. The ipsilateral nociceptive thresholds (paw withdrawal latency; PWL) were tested to evaluate the analgesic effects. Second, 9 healthy male Wistar rats were randomly divided into 3 groups: control group, 2 r/s MA group, and 4 r/s MA group. The proportion of C-fiber neurons (calcitonin gene-related peptide- (CGRP-) positive neurons) and A-fiber neurons (neurofilament 200- (NF200-) positive neurons) in the dorsal root ganglia (DRG) activated by MA were quantitatively analyzed with the morphological immunofluorescence staining method. Third, 30 healthy male Wistar rats were randomly divided into 6 groups: control group, CFA group, CFA + 2 r/s MA group, CFA + 2 r/s MA + RTX group, CFA + 4 r/s MA group, and CFA  + 4 r/s MA + RTX group. Resiniferatoxin (RTX) was injected into the acupoints before acupuncture. PWL was evaluated to investigate the analgesic effect. Results. Both types of MA treatment increased the PWL of saline-injecting rats and pain model rats. Moreover, 4 r/s MA was superior to 2 r/s MA in increasing PWL. A higher quantity of excited C-fiber neurons was observed following 4 r/s MA than 2 r/s MA, while the reverse was observed in the activation of A-fiber neurons. Following the injection of RTX to inhibit the activation of C-fibers, the analgesic effect of 4 r/s MA reduced significantly but not of 2 r/s MA. Conclusion. Strong MA (4 r/s MA) has superior analgesic effects to gentle MA (2 r/s MA) on CFA model rats, which is associated with C-fiber activation.

scholarly journals Inhibitory effect of endostatin gene therapy combined with phosphorus-32 colloid on tumour growth in Wistar rats

2016 ◽  
Vol 36 (3) ◽  
Author(s):  
Huiqi Gao ◽  
Jing Zhu ◽  
Yong Li ◽  
Peng Fu ◽  
Baozhong Shen
Keyword(s):  
Wistar Rats ◽  
Tumour Growth ◽  
Combined Therapy ◽  
Inhibitory Effect ◽  
Saline Control ◽  
Control Group ◽  
Healthy Male ◽  
Saline Control Group ◽  
Male Wistar Rats

Eighty healthy male Wistar rats, aged 5 weeks, weighing 100–120 g, were utilized for establishing tumour-bearing models by immediate Walker-256 cancerous ascites injection and randomly divided to four groups (n=20) treated with 0.2 ml solution containing saline, 32P-colloid (0.3 mCi), endostatin gene (20 μg), endostatin gene combined with colloid 32P. The effect of endostatin combined with a small dose of 32P-colloidal on tumour growth in vivo was evaluated and the potential mechanism underlying the combined therapy was explored. We found that 32P-colloid combined with endostatin exhibited higher inhibitory effect upon tumour growth compared with application of 32P-colloid or endostatin alone, although three therapies all significantly inhibited tumour growth compared with saline control group. The higher inhibitory effect of 32P-colloid combined with endostatin upon tumour growth might be attributed to a synergistic effect of inhibiting angiogenesis by endostatin and inducing apoptosis by 32P-colloid, as demonstrated by microvessel density (MVD) and apoptotic index (AI) measurement. Combined therapy of 32P-colloid and endostatin probably serves as a novel and efficacious therapy of tumour growth.

scholarly journals Utilization of Snakehead Fish Meat for Increasing Kidney Function in Rat Experiencing Physiological Stress

2018 ◽  
Vol 10 (3) ◽  
pp. 553-558
Author(s):  
Sunarno Sunarno ◽  
Hajar Sokmawati ◽  
Rully Rahadian
Keyword(s):  
Body Weight ◽  
Kidney Function ◽  
Wistar Rats ◽  
Physiological Stress ◽  
The Body ◽  
Control Group ◽  
Kidney Weight ◽  
Significance Level ◽  
Fish Meat ◽  
Male Wistar Rats

Physiological stress for a long time can lead to impaired kidney organ function. Snakehead fish meat are known to contain important nutrients such as albumin, glutamine, glycine, cysteine, and various minerals that can improve kidney function. This study was conducted to examine and analyze the effect of snakehead fish supplementation in feed towards body weight, kidney weight, diameter and number of glomerulus in Wistar rats with physiological stress. This study used 20 male Wistar rats divided into 5 groups consisted of 1 control group (P0) and 4 treatment groups (P1: 5%, P2: 10%, P3: 15%, P4: 20%). The treatments were performed with 4 times repetition. This study used Completely Randomized Design (CRD). The variables measured were body weight, kidney weight, diameter and number of renal glomerulus. The data obtained were analyzed using Analysis of Variance (ANOVA) at 5% significance level and continued with Duncan test with 5% significance level. The results of this study were: the supplementation of snakehead fish with 20% of concentration can increase the body weight; concentration 10; 15; and 20% can increase the weight of the kidney; concentrations of 5%, 10%, 15%, and 20% can increase the glomerular diameter in Wistar rats with physiological stress. The supplementation of snakehead fish with concentration of 5% to 20% did not affect the number of glomerulus of Wistar rats with physiological stress. Results of this study is useful as information for people who study the mechanism of kidney repair due to physiological stress.

Zinc and Manganese of serum were negatively, but Copper positively influenced by Iron elevation in diet of male Wistar rats

2018 ◽  
Vol 88 (1-2) ◽  
pp. 50-57
Author(s):  
Ali Zolfaghari ◽  
Hamid Reza Gheisari ◽  
Arash Omidi ◽  
Saeed Nazifi
Keyword(s):  
Serum Iron ◽  
Wistar Rats ◽  
Graphite Furnace ◽  
Serum Zinc ◽  
The Body ◽  
Control Group ◽  
Dietary Iron ◽  
Flame Atomic Absorption ◽  
Iron And Zinc ◽  
Male Wistar Rats

Abstract. The purpose of the study was to determine the effect of dietary iron on distribution of zinc, manganese, copper, calcium and magnesium in the body of Wistar rats. Commercial Sangak, an Iranian traditional flat bread was used in this study. It was prepared by additionof yeast and sourdough. Different doses of iron (35, 70, 140, and 210 mg/kg per diet) were added to bread vehicle for 30 days with or without baking soda (250 mg/kg per diet). The concentrations of the above elements were determined by graphite furnace and flame atomic absorption spectroscopy. Serum zinc and manganese concentrations were significantly lower (P < 0.05) in rats that received higher concentrations of iron compared to the controls. Serum iron, and copper concentrations increased significantly (P < 0.05) with an increase in dietary iron compared to the controls. Increments of dietary concentrations of iron raised the concentration of iron, zinc and manganese in the feces, and lowered the concentration of copper and calcium in the feces (P < 0.05). Diets that contained baking soda showed a statistically significant increase in phytic acid (P < 0.05). Lower serum iron was seen in rats that received baking soda with diet of the control group. Conversely, feces iron and zinc were increased in this group. It is concluded that iron absorption might interfere with zinc and manganese because of similar physicochemical properties. Knowledge about these interactions is essential when supplementation of some elements is recommended in populations with a high risk of some deficiencies such as iron and zinc.

scholarly journals The effect of Dichlorvous (Sniper) Inhalation on the Histology of the Lungs of Adult Male Wistar Rats

2021 ◽  
pp. 58-67
Author(s):  
A. E. Anyabolu ◽  
D. N. Ezejindu ◽  
B. N. Obinwa
Keyword(s):  
Wistar Rats ◽  
The Body ◽  
Control Group ◽  
Male Wistar Rats ◽  
Water Feed ◽  
Group A ◽  
Cervical Dislocation ◽  
Group B ◽  
Histological Architecture ◽  
Group D

The organophosphate, dichlorvous (Otapia-pia) formulated in varying concentrations as insecticides is utilized by several individuals in most remote places of Nigeria due to its affordable value and accessibility. However, this present study is conducted to investigate the adverse effect of the exposure of this substance on the respiratory system (lungs) of male albino wistar rats. Twenty (20) albino wistar rats comprising of all males weighing between 150-230g were divided into four groups (A-D) of five animals each. Group A received only water, feed, and served as the control. Group B was exposed to dichlorvous inhalation 3hrs daily for a period of two weeks; Group C was exposed to dichlorvous inhalation 6hrs daily for a period of two weeks; while Group D was exposed to dichlorvous inhalation 10hrs daily for a period of two weeks. Twenty four hours after the last exposure, the animals were sacrificed by cervical dislocation and dissected. The lungs were weighed and fixed in 10% formal saline for histological studies. The body weight of the experimental groups decreased insignificantly when compared with the control group. The lungs weight increased significantly when compared to the control groups. Histological observation revealed a moderate to severe effects on the lungs with severe consolidated inflammatory exudates (discharge of fluids from pores), a moderate intra-alveoli hemorrhage, and a severe dilation of alveolar septa which is evident as emphysematous changes. This result revealed that the exposure to dichlorvous causes distortion of the normal histological architecture of the lungs. Thus, showing that dichlorvous inhalation influences the normal physiologic mechanisms of the lungs.

scholarly journals THE EFFECT OF RAMANIA LEAF (Bouea macrophylla Griff) EXTRACT GEL ON COLLAGEN FIBERS DENSITY IN INCISIONAL WOUND OF MALE WISTAR RATS

2021 ◽  
Vol 6 (1) ◽  
pp. 96
Author(s):  
Dinda Rizkia ◽  
Irham Taufiqurrahman ◽  
Renie Kumala Dewi
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Collagen Fibers ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Post Hoc

ABSTRACTBackground: Ramania leaf (Bouea macrophylla Griff) extract gel has secondary metabolites in the form of flavonoids, steroids, phenols and terpenoids which have a role as antioxidant. They will protect the body from excessive production of reactive oxygen species (ROS) by increasing endogenous antioxidants SOD, CAT and GPX, so that wound healing will not be inhibited and the process of collagen synthesis can run smoothly. Objective: To analyze the effect of ramania leaf extract gel that was applied topically with 5%, 10% and 15% concentration on collagen fibers density in incisional wound of male Wistar rats (Rattus norvegicus) on the 7th and the 14th day. Method: This research is a pure experimental study with a posttest-only control group design, using 24 rats which were divided into 4 groups: the treatment groups given ramania leaf extract gel of 5%, 10%, 15%, and the control group given placebo gel. The application of the extract gel was done once within 24 hours. The collagen level was measured with a spectrophotometer on the 7th and the 14th day. Results: Two-Way ANOVA test results on the 7th and the 14th day of each group showed a significant difference with p=0.000 (p<0.05). The Bonferroni Post-hoc Test showed a significant difference with p<0.05 between the placebo gel group and the groups of ramania leaf extract gel of 5%, 10%, 15% on the 7th and the 14th day. Conclusion: There is an effect of ramania leaf extract gel on collagen fibers density with the most effective concentration of 15%.Keywords: Antioxidant, , Collagen, Ramania Leaf Extract Gel

scholarly journals Pengaruh Pemberian Calcitriol terhadap Kadar Limfosit pada Tikus Putih (Rattus norvegicus) Galur Wistar Jantan yang Dipapar Asap Rokok

2021 ◽  
Vol 5 (1) ◽  
pp. 29-40
Author(s):  
Jordy Oktobiannobel ◽  
Hetti Rusmini ◽  
Retno Ariza Soeprihatini Soemarwoto ◽  
Syafa’atun Malihah
Keyword(s):  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
White Blood Cells ◽  
The Body ◽  
Control Group ◽  
Placebo Control ◽  
Not Given ◽  
Control Group Design ◽  
Male Wistar Rats

The health impact for active and passive smokers is damage to the organs and respiratory tract. The body organs initially experience inflammation which increases the number of white blood cells, one of which is an increase in lymphocytes. One of the efforts to reduce the damage caused free radical by cigarette smoke is by consuming Calcitriol. Objective: To determine the effect of presenting Calcitriol on lymphocyte levels in male Wistar rats (Rattus norvegicus) exposed to cigarette smoke. Methods: This type of research is a pure experimental (true-experiment) using a pre and post-test research design with a control group design to check lymphocyte levels in male Wistar rats (Rattus norvegicus). 24 rats were divided into 6 groups simultaneously. Random, namely the control group 1 (exposed to cigarette smoke and not given Calcitriol), group 2 (exposed to cigarette smoke and not given Calcitriol), placebo control group (exposed to cigarette smoke and given olive oil), treatment 1 (exposed to cigarette smoke and given Calcitriol with a dose of 0.25 µgr / head), treatment 2 (exposed to cigarette smoke and given Calcitriol at a dose of 0.5 µgr / head) and treatment 3 (exposed to cigarette smoke and given Calcitriol at a dose of 1 µgr / head). Results: Paired t-test lymphocyte levels showed evidence that stated (p <0.05) in the K2 group (p = 0.014), the K3 group (p = 0.049), the P1 group (p = 0.012) and the P3 group (0.001) . Meanwhile, group K1 (p = 0.115) and group P2 (p = 0.066) had no statistical difference. Conclusion: Calcitriol administration can reduce lymphocyte levels in male Wistar rats exposed to cigarette smoke.

scholarly journals Melatonin menurunkan berat badan tetapi tidak menurunkan kadar TNF-α pada tikus wistar jantan yang diberi minyak jelantah selama 28 hari

2017 ◽  
Vol 5 (2) ◽  
pp. 127-132
Author(s):  
Anugrah Novianti ◽  
Edi Dharmana ◽  
Nyoman Suci Widyastiti
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Treated Group ◽  
Waste Cooking Oil ◽  
The Body ◽  
Control Group ◽  
Cooking Oil ◽  
Tnf Α ◽  
Significant Difference ◽  
Male Wistar Rats

Backgound: Non Alcoholic Fatty Liver Disease (NAFLD) occurs when the intake and free fatty acid synthesis occurs more frequently than its oxidation and resecretion in the blood. Melatonin is a powerful antioxidant that can boost the synthesis of endogenous antioxidants in the body, suppress the inflammatory response and inhibit the formation of steatosis.Objective: To analyze the effect of melatonin supplementation in reducing body weight andTNF-α levels in male Wistar rats were fed by waste cooking oil.Methods: True experimental studyusing post-test only control group design. This study was done on 18 male wistar rats were divided into 3 groups : the positive control group (P0) was administrated waste cooking oil, the treated group 1 (P1) was administrated waste cooking oil and 5mg/kgBW melatonin, and the treated group 2 (P2) was administrated waste cooking oil and 10mg/kgBW melatonin for 28 days.Data analysis using One Way ANOVA test and followed by Tukey test to determine the most effective dose of melatonin.Results: There was significant difference in body weightbetween P2group and K0 group (p=0,019) with the mean body weight difference was 19,167g lower than K0 group. There was no difference in TNF-α levels between the three groups (p=0,155). Conclusion: Melatonin dose of 10mg/kgBWloses body weight male Wistar rats have given by waste cooking oil for 28 days, but does not reduce TNF-α levels.

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