scholarly journals Molecular detection of Babesia spp. in ticks sampled from asymptomatic dogs in the area of some Belgrade municipalities

2016 ◽  
Vol 70 (5-6) ◽  
pp. 175-184
Author(s):  
Darko Davitkov ◽  
Srecko Terzic ◽  
Dajana Davitkov ◽  
Milena Radakovic ◽  
Bojan Gajic ◽  
...  
Keyword(s):  
Causative Agent ◽  
Molecular Detection ◽  
Rhipicephalus Sanguineus ◽  
Dermacentor Reticulatus ◽  
Species Determination ◽  
Pcr Rflp ◽  
Causative Agents ◽  
Long Time ◽  
Clinically Significant ◽  
Babesia Spp

Babesiosis of domestic animals is a vector transmissible and clinically significant disease, caused by protozoa of genus Babesia and Theileria. Possible causative agents for this disease in dogs in Europe are: Babesia canis, B. gibsoni, B. vogeli and B. microti-like. Diagnostics of babesiosis of dogs was for a long time based on the visual inspection of stained blood smear under a microscope, while today there have been increasingly used molecular methods of detection in precise, species diagnostics. The objective of this work was molecular detection of the cause of babesiosis of dogs in the ticks sampled from asymptomatic dogs in the region of some Belgrade municipalities, all for better understanding of epizootiological situation. From three sites in Belgrade, there were collected 49 ticks, sampled from the dogs with no symptoms. There was carried out the determination of the ticks, and after that, DNA was isolated for molecular examination. First, there was performed Polymerase Chain Reaction (PCR), for determining the species of the genus Babesia, and after that there was also carried out the determining of polymorphism in the length of restriction fragments (RFLP) for the purpose of the causative agent species determination. Out of the total number of the examined ticks, 18,34% were positive on Babesia spp. By RFLP method, in two cases (4,08%) B. Gibsoni was identified, while in 7 cas?es (14,92%) there were no restriction sites for the used enzymes, what suggests that most likely it was B. canis. The ticks positive on the cause of babesiosis were: Dermacentor reticulatus (4 cases), Rhipicephalus sanguineus (4 cases) i Ixodes ricinus (1 case). This work confirms the presence of Babesia spp. in the ticks sampled from asmptomatic dogs on the teritory of Belgrade as well as the significance of PCR-RFLP method in diagnostics and identification of the causative agent of babesiosis in dogs. For the first time in Serbia, there was determined the presence of B. gibsoni in ticks (Species Rhipicephalus sanguineus)

scholarly journals Molecular detection of Babesia spp. in ticks in northern Serbia

2012 ◽  
Vol 64 (4) ◽  
pp. 1591-1598 ◽  
Author(s):  
D. Mihaljica ◽  
Z. Radulovic ◽  
Snezana Tomanovic ◽  
Sanja Cakic ◽  
Aleksandra Penezic ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Analysis ◽  
Ixodes Ricinus ◽  
Prevalence Rate ◽  
Molecular Detection ◽  
Dermacentor Reticulatus ◽  
Dermacentor Marginatus ◽  
Haemaphysalis Concinna ◽  
Haemaphysalis Punctata ◽  
Babesia Spp

In order to evaluate the prevalence rate of Babesia spp. in ticks collected from vegetation at seven localities in northern Serbia, tick samples were subjected to molecular analysis. A total of 132 unfed adult ticks of five different species (Dermacentor marginatus, Dermacentor reticulatus, Ixodes ricinus, Haemaphysalis concinna and Haemaphysalis punctata), were examined by PCR for the presence of Babesia spp. Out of the analyzed ticks, 10.61% (14/132) were positive for babesial DNA. The presence of babesiae was found at the localities Pancevacki Rit, Titov Gaj, Makis, PKB and Kljajicevo. Prevalence in D. reticulatus ticks was 21.57% (11/51) and in H. concinna ticks, 8.57% (3/35). Sequencing and phylogenetic analysis showed a clustering of the obtained sequences with those of B. canis from the GenBank database. These results add to the knowledge of the distribution of babesial pathogens and their vectors in Serbia.

scholarly journals Challenges in Tick-Borne Pathogen Detection: The Case for Babesia spp. Identification in the Tick Vector

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 92
Author(s):  
Grecia Martínez-García ◽  
R. Montserrat Santamaría-Espinosa ◽  
José J. Lira-Amaya ◽  
Julio V. Figueroa
Keyword(s):  
Tick Species ◽  
Pathogen Detection ◽  
Molecular Tools ◽  
Conventional Pcr ◽  
Tissue Samples ◽  
Tick Vector ◽  
Pcr Rflp ◽  
Causative Agents ◽  
Tick Vectors ◽  
Babesia Spp

The causative agents of Babesiosis are intraerythrocytic protozoa of the genus Babesia. Babesia parasites are present around the world, affecting several mammals including humans, pets and livestock, hence its medical and veterinary relevance. Babesia spp. detection in its invertebrate host is a main point in understanding the biology of the parasite to acquire more knowledge on the host–Babesia–vector interactions, as increasing knowledge of the Babesia lifecycle and babesiosis epidemiology can help prevent babesiosis outbreaks in susceptible mammals. The aim of the present review is to highlight the newest findings in this field, based on a bibliographic compilation of research studies recently carried out for the detection of the main Babesia species found in tick vectors affecting mammalian hosts, including the different tick stages such as adult ticks, larvae, nymphs and eggs, as well as the detection method implemented: microscopic tools for parasite identification and molecular tools for parasite DNA detection by conventional PCR, nested-PCR, PCR-RFLP, PCR-RLB hybridization, real time-PCR, LAMP and RAP assays. Although molecular identification of Babesia parasites has been achieved in several tick species and tissue samples, it is still necessary to carry out transmission experiments through biological models to confirm the vectorial capacity of various tick species.

scholarly journals Incidence of Canine Tick Vectors and Molecular Detection of Haemoparasites in Vectors and Hosts

2021 ◽  
Author(s):  
A. Jena ◽  
S. Baidya ◽  
S. Pandit ◽  
R. Jas ◽  
S.C. Mandal ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Tick Infestation ◽  
Rhipicephalus Sanguineus ◽  
Hepatozoon Canis ◽  
Babesia Gibsoni ◽  
Veterinary Clinic ◽  
Primer Sets ◽  
One Year ◽  
Tick Vectors ◽  
Babesia Spp

Background: Ticks are of great importance in transmission of various canine tick borne diseases. Several characteristics of ticks make them outstanding vectors of pathogenic agents, the wide host range and slow feeding habit along with tendency to feed on several hosts during life cycle ensures ample opportunity to acquire and transmit pathogens. Methods: This study focuses on status of canine tick vectors and molecular detection of haemoparasites in these ticks and their host, in and around Kolkata. The blood and tick samples were collected from Dog Ward, Department of Teaching Veterinary and Clinical Complex; Faculty of Veterinary and Animal Sciences, WBUAFS at Belgachia; Veterinary Clinic of Kolkata Police Dog Squad at Alipore; Veterinary Clinic of Barrackpore Police dog Squad and samples from stray dogs were also collected from inside the University campus through a period of one year (August, 2016 to July, 2017). Result: The tick infestation was recorded at 41% with Rhipicephalus sanguineus, being the only tick. Nine primer sets were used for detection of Babesia spp, Ehrlichia canis, Ehrlichia chaffensis, Babesia gibsoni, Hepatozoon canis, Mycoplasma haemocanis, Anaplasma platys and Theileria annae from the respective tick samples and blood sample of hosts. Tick samples were found positive for Babesia spp, Ehrlichia spp. and H. canis where as the corresponding blood samples were found positive for Babesia spp, Ehrlichia spp., Mycoplasma spp. and H. canis. This study conclusively provides evidence of high rates of incidence of haemoparasitic infection or canine tick borne diseases infection and tick infestation, with at least four haemoparasites infecting the dog population and at least one tick species (Rhipicephalus sanguineus) infesting the dogs in and around Kolkata.

scholarly journals Molecular Detection of Zoonotic and Non-Zoonotic Pathogens from Wild Boars and Their Ticks in the Corsican Wetlands

Pathogens ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1643
Author(s):  
Baptiste Defaye ◽  
Sara Moutailler ◽  
Christian Pietri ◽  
Clemence Galon ◽  
Sébastien Grech-Angelini ◽  
...  
Keyword(s):  
Wild Boar ◽  
Animal Species ◽  
Molecular Detection ◽  
Rhipicephalus Sanguineus ◽  
Animal Population ◽  
Blood Samples ◽  
Wild Boars ◽  
On This Island ◽  
First Time ◽  
Babesia Spp

Corsica is the main French island in the Mediterranean Sea and has high levels of human and animal population movement. Among the local animal species, the wild boar is highly prevalent in the Corsican landscape and in the island’s traditions. Wild boars are the most commonly hunted animals on this island, and can be responsible for the transmission and circulation of pathogens and their vectors. In this study, wild boar samples and ticks were collected in 17 municipalities near wetlands on the Corsican coast. A total of 158 hunted wild boars were sampled (523 samples). Of these samples, 113 were ticks: 96.4% were Dermacentor marginatus, and the remainder were Hyalomma marginatum, Hyalomma scupense and Rhipicephalus sanguineus s.l. Of the wild boar samples, only three blood samples were found to be positive for Babesia spp. Of the tick samples, 90 were found to be positive for tick-borne pathogens (rickettsial species). These results confirm the importance of the wild boar as a host for ticks carrying diseases such as rickettsiosis near wetlands and recreational sites. Our findings also show that the wild boar is a potential carrier of babesiosis in Corsica, a pathogen detected for the first time in wild boars on the island.

Fungus Spaeropsis Malorum peck. One of the currant stalk drying agents

2020 ◽  
Vol 62 ◽  
pp. 172-178
Author(s):  
S. E. Golovin
Keyword(s):  
Causative Agent ◽  
High Frequency ◽  
Frequency Of Occurrence ◽  
Ribes Nigrum ◽  
Black Currant ◽  
Causative Agents ◽  
Pear Trees ◽  
Red Currant

The article highlights the results of research carried out in 2019-2020 to study the reasons for the drying out of the stems of black currant (Ribes nigrum L.), red currant (Ribes rubrum L.) and golden currant (Ribes aureum L.). As a result of research, the fungus Sphaeropsis malorum Peck. in the first in Russia it was revealed from the drying stems of three types of black currants, red currants and golden currants. This  pathogen inhabited currant stems together with fungi from the genus Cytospora spp., causative agents of stem drying. On red currants, it was established that S. malorum was the main causative agent of branch drying, as indicated by the high frequency of occurrence (75.0-83.3 %) of this fungus on the affected stems, compared with species from the genus Cytospora spp. (16.7-33.3 %) and Phomopsis ribesia (25.0 %). Due to the fact that the cultivation of currants in the immediate vicinity of apple and pear trees leads to infection of plants with the fungus S. malorum, it is necessary to avoid planting currants in the vicinity of fruit-bearing seed gardens.

Respiratory tract damage with bocavirus infection in children

2020 ◽  
Vol 19 (2) ◽  
pp. 14-18
Author(s):  
E. V. Sharipova ◽  
I. V. Babachenko ◽  
M. A. Shcherbatyh
Keyword(s):  
Respiratory Tract ◽  
Lower Respiratory Tract ◽  
Clinical Manifestations ◽  
Respiratory Viruses ◽  
Community Acquired Pneumonia ◽  
Human Bocavirus ◽  
Long Time ◽  
Clinically Significant ◽  
Tract Infections ◽  
Tract Damage

Long time the main pathogens associated with the development of community-acquired pneumonia were bacteria. However, in recent years in the Russian Federation, like all over the world, the view of the damage of lower respiratory tract changed, including a unique approach to community-acquired pneumonia as a bacterial infection, and respiratory viruses have become seen as a direct cause of lower respiratory tract damage, or as part of a viral-bacterial co-infection. These studies became possible since the widespread introduction of PCR techniques in the clinical setting, identification of respiratory viruses has increased and new microorganisms such, one as human bocavirus have been discovered. Objective: to study the features of respiratory tract damage in acute bocavirus infection in children of different ages. Materials and methods: A retrospective analysis of 97 medical hospital documentation of children with acute bocavirus infection, detected confirmed by PCR in nasopharyngeal aspirate. Results: In this work, it was shown that human bocavirus spread throughout the year with an increase in the incidence of clinically significant forms in the autumnwinter period, including during the period of an increase in the incidence of influenza. HBoV infection requiring hospitals is most significant in the first three years of life. In 74.2% of hospitalized children, bocavirus infection occurs with lower respiratory tract infections in the form of bronchitis — 77.8%, pneumonia — 28.9% and rarely bronchiolitis and is complicated by the development of respiratory failure in 28.9% of cases. Changes in the blood test are non-specific, and the level of C-reactive protein in children with various clinical manifestations of HBoV infection generally does not exceed 50 mg / l. An x-ray of the chest organs does not objectively reflect the existing volume and nature of the inflammatory process in the lungs.

Bulinus species on Madagascar: molecular evolution, genetic markers and compatibility with Schistosoma haematobium

Parasitology ◽  
2001 ◽  
Vol 123 (7) ◽  
pp. 261-275 ◽  
Author(s):  
J. R. STOTHARD ◽  
P. BRÉMOND ◽  
L. ANDRIAMARO ◽  
B. SELLIN ◽  
E. SELLIN ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Ribosomal Subunit ◽  
Population Genetic Analysis ◽  
Basal Node ◽  
Pcr Rflp ◽  
Dna Sequence Variation ◽  
Long Time ◽  
Laboratory Infection ◽  
Species Groups ◽  
Nucleotide Divergence

Of the four species of Bulinus found on Madagascar, three species: B. obtusispira, B. liratus and B. bavayi are endemic while the fourth, B. forskalii, is probably a recent introduction from the African mainland. The evolutionary relationships of these species with Bulinus species from Africa were studied by phylogenetic analysis of DNA sequence variation at two mitochondrial loci: cytochrome oxidase subunit I (COI) and large ribosomal subunit (LSU) or 16S. The observed levels of nucleotide divergence within Bulinus were substantial but may underestimate the true levels as there was evidence of ‘saturation' of transitional substitutions at both loci. A putative secondary structure model for the sequenced segment of the 16S was developed. Subsequent phylogenetic analysis using transversional changes only for both loci, showed that there were contrasting levels of divergence within the four species groups. B. obtusispira was consistently placed within the B. africanus group, appearing ancestral to this group and was closest to the basal node within Bulinus. Together with B. bavayi, the two species appear to have been isolated on Madagascar for a long time, contrasting with both B. liratus and B. forskalii that appear more recent colonisers; however, estimate of exact times of divergence is problematic. A PCR-RFLP assay was developed to enable identification and discrimination of B. obtusispira and B. liratus using discriminatory variation within the COI. To enable population genetic analysis within B. obtusispira, microsatellite markers were developed using an enrichment method and 8 primer pairs are reported. Laboratory infection experiments using Madasgacan S. haematobium from the Mahabo area showed that certain populations of B. obtusispira, B. liratus and B. bavayi were compatible.

scholarly journals Molecular detection of tick-borne pathogens in canine population and Rhipicephalus sanguineus (sensu lato) ticks from southern Metro Manila and Laguna, Philippines

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Remil L. Galay ◽  
Anna Angelica L. Manalo ◽  
Sidney Lyndon D. Dolores ◽  
Irene Pearl M. Aguilar ◽  
Kristina Andrea C. Sandalo ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Rhipicephalus Sanguineus ◽  
Canine Population ◽  
Rhipicephalus Sanguineus Sensu Lato ◽  
Metro Manila

scholarly journals Composition of tick species (Acari: Ixodida) on dogs in the urban agglomeration – a multi-year study

2017 ◽  
Vol 73 (11) ◽  
pp. 698-701 ◽  
Author(s):  
Mirosław M. Michalski
Keyword(s):  
Ixodes Ricinus ◽  
Tick Species ◽  
Dominant Species ◽  
Rhipicephalus Sanguineus ◽  
Dermacentor Reticulatus ◽  
Urban Agglomeration ◽  
The Subject ◽  
The City

Ticks parasitizing on dogs belong to mites of the order Ixodida, class Arachnida. In Poland the occurrence of four species has been recorded most frequently: Ixodes ricinus, I. hexagonus, I. crenulatus and Dermacentor reticulatus. Their importance is due to the fact that they are vectors of many dangerous diseases of humans and animals. The subject of the present study were ticks collected from dogs, patients of several veterinary clinics from the urban agglomeration of the city of Olsztyn. Ticks were collected from May to June in eight consecutive years: 2009–2016. The dominant species was I. ricinus (60.14%) in relation to D. reticulatus (39.71%). In two consecutive years, 2011–2012, single females of Rhipicephalus sanguineus were also identified, an exceptional finding of this species in our country. In D. reticulatus, nymphs predominated over females (23.74% vs 16.0%) while in I. ricinus the respective percentage was 29.6% and 30.55%. However, in subsequent years of the study the proportions between nymphs and females varied greatly in both species.

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