scholarly journals Molecular detection of Babesia spp. in ticks in northern Serbia

2012 ◽  
Vol 64 (4) ◽  
pp. 1591-1598 ◽  
Author(s):  
D. Mihaljica ◽  
Z. Radulovic ◽  
Snezana Tomanovic ◽  
Sanja Cakic ◽  
Aleksandra Penezic ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Analysis ◽  
Ixodes Ricinus ◽  
Prevalence Rate ◽  
Molecular Detection ◽  
Dermacentor Reticulatus ◽  
Dermacentor Marginatus ◽  
Haemaphysalis Concinna ◽  
Haemaphysalis Punctata ◽  
Babesia Spp

In order to evaluate the prevalence rate of Babesia spp. in ticks collected from vegetation at seven localities in northern Serbia, tick samples were subjected to molecular analysis. A total of 132 unfed adult ticks of five different species (Dermacentor marginatus, Dermacentor reticulatus, Ixodes ricinus, Haemaphysalis concinna and Haemaphysalis punctata), were examined by PCR for the presence of Babesia spp. Out of the analyzed ticks, 10.61% (14/132) were positive for babesial DNA. The presence of babesiae was found at the localities Pancevacki Rit, Titov Gaj, Makis, PKB and Kljajicevo. Prevalence in D. reticulatus ticks was 21.57% (11/51) and in H. concinna ticks, 8.57% (3/35). Sequencing and phylogenetic analysis showed a clustering of the obtained sequences with those of B. canis from the GenBank database. These results add to the knowledge of the distribution of babesial pathogens and their vectors in Serbia.

Effects of climate change on babesiosis vectors.

2021 ◽  
pp. 223-227
Author(s):  
Jeremy Gray
Keyword(s):  
Climate Change ◽  
Ixodes Ricinus ◽  
Climate Change Impact ◽  
Dermacentor Reticulatus ◽  
Impact Of Climate Change ◽  
Climate Change Effects ◽  
Haemaphysalis Punctata ◽  
Change Impact ◽  
Abundance And Distribution ◽  
The Impact

Abstract This chapter discusses the impact of climate change on the abundance and distribution of babesiosis vectors and, by implication, transmission of Babesia spp. It discusses evidence for climate change impact on the vectors Ixodes ricinus, Dermacentor reticulatus, Haemaphysalis punctata and Hyalomma spp. as well as the absence of evidence of the same climate change effects on the vectors Rhipicephalus spp. and I. scapularis.

scholarly journals Occurrence of juvenile Dermacentor reticulatus ticks in three regions in Poland: the final evidence of the conquest

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Dorota Dwużnik-Szarek ◽  
Ewa Julia Mierzejewska ◽  
Anna Bajer
Keyword(s):  
Ixodes Ricinus ◽  
Tick Species ◽  
Dermacentor Reticulatus ◽  
Tick Population ◽  
Western Region ◽  
Eastern Region ◽  
Haemaphysalis Concinna ◽  
Fallow Lands ◽  
The Western Region ◽  
June July

Abstract Background Two populations of Dermacentor reticulatus ticks (Western and Eastern) in Poland are among the most dynamic tick populations in Central Europe. Expansion and settlement of ticks in new localizations depend on the presence of suitable hosts, for both adult and juvenile ticks. Methods The current study was planned to complement our previous studies on questing adult ticks and was focused on a collection of juvenile D. reticulatus ticks from rodents from three regions in Poland, defined by the presence/absence of adult ticks (regions of the Western and Eastern tick population and the gap area between them) to confirm the existence of stable populations. Rodent trapping was conducted in open habitats (fallow lands, wasteland and submerged meadows) in 2016–2018 in June, July and/or August to encompass seasonal peaks of larvae and nymph activity. Results Altogether, three tick species were collected, 2866 D. reticulatus, 2141 Ixodes ricinus and 427 Haemaphysalis concinna. Dermacentor reticulatus was the most common (72.3%) and abundant (mean 17.94 ± 2.62 ticks/rodent) tick species on rodents from the Eastern region; in the Western region infestation of rodents was only 6.8%. Ixodes ricinus was found in all three regions and was the only tick species collected from rodents from the gap area. Haemaphysalis concinna was noted only in the Western region. The highest infestation of juvenile D. reticulatus was recorded on voles (Myodes and Microtus spp.), infestation of I. ricinus was the highest on Apodemus mice, and the majority of H. concinna ticks were collected from root voles Alexandromys oeconomus. Conclusions Our study confirmed a stable population of D. reticulatus in Eastern and Central Poland and a lower prevalence and mean abundance of this tick species among rodents from the Western region. A lack of juvenile D. reticulatus on rodents in Niewiadów confirmed the existence of the gap area, free of D. reticulatus ticks. Graphical abstract

Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic

2016 ◽  
Vol 115 (10) ◽  
pp. 3897-3904 ◽  
Author(s):  
Zuzana Hamšíková ◽  
Cornelia Silaghi ◽  
Ivo Rudolf ◽  
Kristýna Venclíková ◽  
Lenka Mahríková ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Czech Republic ◽  
Ixodes Ricinus ◽  
Molecular Detection ◽  
Ixodes Ricinus Ticks

Prevalence and phylogenetic analysis of Babesia spp. in Ixodes ricinus and Ixodes persulcatus ticks in Latvia

2015 ◽  
Vol 68 (3) ◽  
pp. 325-336 ◽  
Author(s):  
Valentina Capligina ◽  
Inese Berzina ◽  
Antra Bormane ◽  
Ineta Salmane ◽  
Karlis Vilks ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Ixodes Ricinus ◽  
Ixodes Persulcatus ◽  
Babesia Spp

Investigation of Babesia spp. in sympatric populations of Dermacentor reticulatus and Ixodes ricinus ticks in Lithuania and Latvia

2018 ◽  
Vol 9 (2) ◽  
pp. 270-274 ◽  
Author(s):  
Jana Radzijevskaja ◽  
Dalytė Mardosaitė-Busaitienė ◽  
Asta Aleksandravičienė ◽  
Algimantas Paulauskas
Keyword(s):  
Ixodes Ricinus ◽  
Dermacentor Reticulatus ◽  
Sympatric Populations ◽  
Ixodes Ricinus Ticks ◽  
Babesia Spp

scholarly journals Tick Fauna of Small Ruminants in South Part of Serbia, with Emphasis to North Kosovo

2020 ◽  
Vol 77 (1) ◽  
pp. 38
Author(s):  
Ivan PAVLOVIĆ ◽  
Valentina MILANOVIĆ ◽  
Bisa RADOVIĆ ◽  
Snežana IVANOVIĆ ◽  
Milan P. PETROVIĆ ◽  
...  
Keyword(s):  
Ixodes Ricinus ◽  
Tick Species ◽  
Small Ruminant ◽  
Small Ruminants ◽  
Rhipicephalus Sanguineus ◽  
The South ◽  
Dermacentor Marginatus ◽  
Haemaphysalis Punctata

The study regarding tick fauna and season distribution of ticks of small ruminant in the south part of Serbia, with emphasis on north Kosovo was performed during 2017. During the study we examined a total of 114 flocks of goats and sheep from Zvečan and Leposavić districts (villages Ceranja, Majdevo, Zemanica, Mure, Rudine, Žitkovac, Oraovica, Mošnica, Donji Krnjin, Belo brdo, Mioliće, Drenova and Beliće). Infections occurred at and on 56.14% of examined sheep and 31.42% of examined goats. The most abudant tick species were Ixodes ricinus, followed by Dermacentor marginatus, Rhipicephalus sanguineus, R.bursa, Haemaphysalis punctata and D.recticulatus.

scholarly journals Molecular detection of Babesia spp. in ticks sampled from asymptomatic dogs in the area of some Belgrade municipalities

2016 ◽  
Vol 70 (5-6) ◽  
pp. 175-184
Author(s):  
Darko Davitkov ◽  
Srecko Terzic ◽  
Dajana Davitkov ◽  
Milena Radakovic ◽  
Bojan Gajic ◽  
...  
Keyword(s):  
Causative Agent ◽  
Molecular Detection ◽  
Rhipicephalus Sanguineus ◽  
Dermacentor Reticulatus ◽  
Species Determination ◽  
Pcr Rflp ◽  
Causative Agents ◽  
Long Time ◽  
Clinically Significant ◽  
Babesia Spp

Babesiosis of domestic animals is a vector transmissible and clinically significant disease, caused by protozoa of genus Babesia and Theileria. Possible causative agents for this disease in dogs in Europe are: Babesia canis, B. gibsoni, B. vogeli and B. microti-like. Diagnostics of babesiosis of dogs was for a long time based on the visual inspection of stained blood smear under a microscope, while today there have been increasingly used molecular methods of detection in precise, species diagnostics. The objective of this work was molecular detection of the cause of babesiosis of dogs in the ticks sampled from asymptomatic dogs in the region of some Belgrade municipalities, all for better understanding of epizootiological situation. From three sites in Belgrade, there were collected 49 ticks, sampled from the dogs with no symptoms. There was carried out the determination of the ticks, and after that, DNA was isolated for molecular examination. First, there was performed Polymerase Chain Reaction (PCR), for determining the species of the genus Babesia, and after that there was also carried out the determining of polymorphism in the length of restriction fragments (RFLP) for the purpose of the causative agent species determination. Out of the total number of the examined ticks, 18,34% were positive on Babesia spp. By RFLP method, in two cases (4,08%) B. Gibsoni was identified, while in 7 cas?es (14,92%) there were no restriction sites for the used enzymes, what suggests that most likely it was B. canis. The ticks positive on the cause of babesiosis were: Dermacentor reticulatus (4 cases), Rhipicephalus sanguineus (4 cases) i Ixodes ricinus (1 case). This work confirms the presence of Babesia spp. in the ticks sampled from asmptomatic dogs on the teritory of Belgrade as well as the significance of PCR-RFLP method in diagnostics and identification of the causative agent of babesiosis in dogs. For the first time in Serbia, there was determined the presence of B. gibsoni in ticks (Species Rhipicephalus sanguineus)

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