scholarly journals Impact of field dodder (Cuscuta campestris Yunk.) on physiological and anatomical changes in untreated and herbicide-treated alfalfa plants

2016 ◽  
Vol 31 (3-4) ◽  
pp. 115-120 ◽  
Author(s):  
Marija Saric-Krsmanovic ◽  
Dragana Bozic ◽  
Ljiljana Radivojevic ◽  
Jelena Gajic-Umiljendic ◽  
Sava Vrbnicanin

The effects of field dodder on physiological processes and the anatomy of alfalfa plants were examined under controlled conditions. The experiment included the following variants: N - noninfested alfalfa plants (control); I - infested alfalfa plants (untreated); T - infested plants treated with imazethapyr. Imazethapyr application rate was 100 g a.i. ha-1. The following parameters were checked: physiological - pigment content (chlorophyll a, chlorophyll b, total carotenoids); anatomical - stem parameters: thickness of epidermis and cortex, and diameter of stem and central cylinder; leaf parameters: thickness of epidermis, parenchyma and spongy tissue, mesophyll and underside leaf epidermis, and diameter of bundle sheath cells in alfalfa plants. Pigment contents and anatomical parameters were measured: prior to herbicide treatment (0 assessment), then 7 (I assessment), 14 (II assessment), 21 (III assessment), 28 (IV assessment) and 35 (V assessment) days after application (DAA). Field dodder was found to affect the contents of chlorophyll a, chlorophyll a and carotenoids in untreated alfalfa plants, causing significant reductions in pigment content. Conversely, percent reduction in the treated plants decreased 22-5% for chlorophyll a, 25-1%, for chlorophyll b, and 21-11% for carotenoids, while a stimulating effect of 1-6% was observed for the contents of chlorophyll b and carotenoids 35 DAA. Plants infested (untreated) by field dodder had lower values of most anatomical parameters, compared to noninfested plants. The measured anatomical parameters of alfalfa stems and leaves had significantly higher values in noninfested plants and plants treated with imazethapyr than in untreated plants.

1973 ◽  
Vol 37 ◽  
Author(s):  
N. Lust

Pigment content of ashes grown up under different circumstances - The pigment content (chlorophyll a, chlorophyll b,  xanthophyll and carotene) has been researched with ashes grown up under  different light circumstances and varying in age and height.     The results prove that the general laws concerning the influence of light  on the pigment content, don’t always work.     The phenomen is very complex. The light quantity is very important in some  cases, but insignificant in others. It seems origin and height of plants have  a strong influence. The results prove also the influence of the environment  is much higher on small plants as on big ones.     The research indicates finally the correlation between the green pigments,  the yellow pigments, and between the green pigments on the one side and the  yellow ones on the other side.


2019 ◽  
Vol 99 (2) ◽  
pp. 199-209 ◽  
Author(s):  
Marija Saric-Krsmanovic ◽  
Dragana Bozic ◽  
Ljiljana Radivojevic ◽  
Jelena Gajic Umiljendic ◽  
Sava Vrbnicanin

The physiological and anatomical impact of field dodder (Cuscuta campestris Yunck.) on alfalfa and sugar beet was examined under controlled conditions. The following parameters were checked: physiological — content of pigments (chlorophyll a, chlorophyll b, and carotenoids) and mineral nutrients: nitrogen, phosphorus, potassium, and percent of organic and mineral nutrients; and anatomical — thickness of the epidermis and cortex, diameter of the stem and central cylinder of alfalfa plants, diameter of tracheids and phloem cells, area of xylem and phloem, and hydraulic conductance of petiole bundles in petiole vascular bundles of sugar beet plants. Leaf parameters were also measured on both host plants: thickness of upper and underside leaf epidermis, thickness of palisade, spongy and mesophyll tissue, and diameter of vascular bundle cells. Pigments content and anatomical parameters were measured 7, 14, 21, 28, 35, and 42 d after infestation (DAI), while mineral nutrient contents were determined 20 and 40 DAI. Field dodder caused a significant reduction in pigments content in infested alfalfa (15%–68%) and sugar beet plants (1%–54%). The results obtained in this study confirmed that this parasitic flowering plant has a strong effect on most anatomical parameters of the stem and leaf of alfalfa and leaf and petiole of sugar beet. Also, it was revealed that field dodder increased the contents of N, P2O5, K2O, and organic nutrients in infested alfalfa plants, while infested sugar beet plants had higher contents of N and organic nutrients compared with non-infested plants.


1995 ◽  
Vol 27 (2) ◽  
pp. 155-160
Author(s):  
M. A. Sanchez-Hoyos ◽  
E. Manrique

AbstractThalli of Ramalina capitata were sprayed with deionized water, 10 mM solutions of KNO3 or NH4Cl, or maintained without any water supply, for 10 days under controlled conditions. The lichens without a water supply showed an increase n antheraxanthin, lutein and β-carotene. The samples treated with deionized water showed a significant increase in violaxathin, antheraxanthin and β-carotene. The lack of a significant difference in zeaxanthin could indicate the absence of de-epoxidation of violaxanthin to zeaxanthin via antheraxanthin. Those samples treated with nitrate solution showed a significant increase in all pigments except for antheraxanthin. In contrast, ammonium treatment did not induce changes compared to water alone, except that antheraxanthin decreased. The lichens treated with nitrate exhibited a significant increase in both chlorophyll a and chlorophyll b, but ammonium affected only chlorophyll b.


Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2065 ◽  
Author(s):  
Woo Park ◽  
Hye-Jin Kim ◽  
Min Li ◽  
Dong Lim ◽  
Jungmin Kim ◽  
...  

Arthrospira platensis is the widely available source of spirulina that contains distinctive natural pigments, including carotenoids and C-phycocyanin (C-PC). In this study, the major carotenoid and C-PC contents were determined in seven commercially available spirulina powder products and laboratory-prepared A. platensis trichomes (AP-1) by an LC-DAD method and UV-Visible spectrometry, respectively. The correlation of these two pigment content levels with Hunter color coordinates and antioxidant activity was also evaluated. The L* value failed to show a significant correlation with pigment content, but a positive correlation was observed between a* values and the contents of total carotenoid and C-PC. As b* values decreased, the chlorophyll a and C-PC contents increased. AP-1 exhibited the highest content of total carotenoids, chlorophyll a and C-PC, and antioxidant activities among the samples. This observation could be related to degradation of these pigments during the mass production process. The carotenoid profiles suggested that the commercial spirulina powders originated from two different sources, A. platensis and A. maxima. Total carotenoid and C-PC content exhibited positive significant correlations with antioxidant activities measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays. These results provide a strong scientific foundation for the establishment of standards for the commercial distribution of quality spirulina products.


2021 ◽  
Vol 16 (1) ◽  
pp. 53-63
Author(s):  
Q Ayub ◽  
S M Khan ◽  
I Hussain ◽  
K Naveed ◽  
S Ali ◽  
...  

A pot experiment was conducted to evaluate the adverse effects of drought on different okra cultivars and to identify the most suitable okra cultivar for growing in drought conditions. Five okra cultivars namely Pusa Green, Clemson, Sabz Pari, Pusa Swani and Mehak Pari were subjected to three drought levels i.e., control (100% Field capacity),50% and 25% Field Capacity (FC). Physiological parameters like fresh and dry weight of plant and plant height were recorded along with biochemical attributes such as chlorophyll content (a, b, total) carotenoids, total protein, proline content, and Membrane stability index (MSI%). Results showed that drought significantly reduced all the studied parameters and at maximum drought (25% FC) lowest values of fresh weight (12.42g), dry weight (1.22g), plant height (7.86cm), chlorophyll a (9.02mg/g FW), chlorophyll b (18.69mg/g FW), total chlorophyll (27.71mg/g FW), carotenoids (11.80mg/g FW), total protein (2.73mg/g FW),whereas maximum Proline (21.36μg/g FW), and MSI (72%) were observed under the same drought. The results concerning responses of okra cultivars under drought conditions showed that maximum. Fresh weight (15.25g) and Dry weight (2.74g) was observed in Pusa green while maximum Plant height (13.77cm), Chlorophyll a (14.38mg/g FW), Chlorophyll b (24.41mg/g FW), Total Chlorophyll (38.80mg/g FW), Carotenoids (18.57mg/g FW), Total Protein (5.44mg/g FW), Proline (27.78μg/g FW), and MSI (56.33%) were produced by Sabz Pari. Hence it can be concluded that drought causes significant variation on physical and biochemical attributes of okra whereas Sabz Pari showed resistance towards the applied stress and produced better results.


Author(s):  
Manohar Lal Meghwal ◽  
M.L. Jyothi ◽  
P.B. Pushpalatha ◽  
Jyothi Bhaskar ◽  
V.I. Beena ◽  
...  

Background: Chlorophyll is a green molecule found in plant cells that aids photosynthesis. It absorbs sunlight and converts the energy into carbohydrates using CO2 and water as a starting point. Chlorophyll ‘a’ and chlorophyll ‘b’ are the two forms of chlorophyll found in plants and each serves as a photoreceptor in photosynthesis, assuming higher electron transport, thus improving photochemical capacity. Methods: The present study was conducted at Banana Research Station, Kannara, Kerala Agricultural University, Thrissur, Kerala during 2017-2018 to elucidate the response of banana Musa (AAB) ‘Nendran’ in terms of growth, yield and quality to nutrient sources.Variation in leaf parameters, phyllochron and chlorophyll content in leaf tissue of banana raised with different nutrient sources was analysed. The amount of chlorophyll present in the leaves, is an indication of photosynthetic efficiency. Result: Highest Leaf Area Index (2.34) were recorded in T4 (integrated use of organic manures with biofertilzers practised). Highest amount of chlorophyll ‘a’ (1.20 mg), chlorophyll ‘b’ (1.62 mg), total chlorophyll (2.82 mg) and lowest phyllochron (7.33) were obtained in T8 (Fertigation with organic sources FYM @29 kg, lime @0.5 kg and wood ash @4 kg as basal; fertigation once in four days with 14 kg FYM till one month after bunch emergence+ in situ green manuring) which was followed T3 (POP recommendation of KAU with organic manures FYM @15kg + lime @0.5 kg as basal + Poultry manure @ 14 kg/plant + Wood ash @4kg/plant applied in two splits i.e. one as basal and one 3 MAP + in situ green manuring) at 150 days after planting. The results indicated that the manuring Nendran banana with organic manures had advantage over chemical fertilizers in term of chlorophyll production and increase in photosynthetic efficiency.


2020 ◽  
Vol 23 (2) ◽  
pp. 225-233
Author(s):  
Martice Desi Pesang ◽  
James Ngginak ◽  
Alfred Gasper Onisimus Kase ◽  
Coni Lisandra Balle Bisilissin

Indonesia has marine biological natural resources consisting of fish, seaweed, crabs and shrimp which are very potential. The purpose of this study was to determine the composition of pigments in Ulva sp (Chlorophyta), Padina australis (Paeophyta) and Hypnea sp (Rhodophyta) obtained from Tablolong waters. The method used in this research is descriptive qualitative and quantitative. Proof of pigment in samples using thin layer chromatography (TLC). The results of pigment analysis of Ulva sp obtained twelve color spots with identified pigment types consisting of chlorophyll a, chlorophyll b, xanthophyll, b-carotene and feofitin. The pigment content identified in Padina australis samples are chlorophyll b, feoforbid, xanthophyll, chlorophyll a, carotene and feofitin a. The types of pigments identified in Ulva sp include pigment chlorophyll a, chlorophyll b, carotene and feofitin from the total color spots formed. Based on the results of this study concluded that non-cultivated seaweed obtained from Tablolong waters contains carotenoid and chlorophyll pigments. Suggestions from this study are expected to do an analysis of pigment content analysis in the dry season time period and continued with antioxidant testing.  Indonesia memiliki Sumber Daya Alam hayati laut yang terdiri dari ikan, rumput laut, kepiting dan udang yang sangat potensial. Tujuan penelitian ini adalah untuk mengetahui komposisi pigmen pada Ulva sp (Chlorophyta), Padina australis (Paeophyta)  dan Hypnea sp (Rhodophyta)  yang diperoleh dari perairan Tablolong. Metode yang digunakan dalam penelitian ini adalah deskriptif kualitatif dan kuantitatif. Pembuktian pigmen pada sampel menggunakan Kromatografi lapis tipis (KLT). Hasil analisis pigmen dari Ulva sp diperoleh dua belas spot warna dengan jenis pigmen yang teridentifikasi terdiri dari klorofil a, klorofil b, xantofil, b-karoten dan feofitin. Kandungan pigmen yang teridentifikasi pada sampel Padina australis adalah klorofil b, feoforbid, xantofil, klorofil a, karoten dan feofitin a. Jenis pigmen yang teridentifikasi pada Ulva sp meliputi pigmen klorofil a, klorofil b, karoten dan feofitin dari total spot warna yang terbentuk. Berdasarkan hasil penelitian dapat disimpulkan bahwa rumput laut non budidaya yang diperoleh dari perairan Tablolong memiliki kandungan pigmen karotenoid dan klorofil. Saran dari penelitian ini diharapkan dilakukan peneltian analisis kandungan pigmen pada periode waktu musim kemarau serta dilanjutkan dengan uji antioksidan.


Forests ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1197
Author(s):  
Waldemar Zielewicz ◽  
Barbara Wróbel ◽  
Gniewko Niedbała

The aim of the study was to identify and compare the content of chlorophyll and carotene pigments in mountain melick plants (Melica nutans L.) growing in two forest habitats in the Zielonka Forest Landscape Park in the Greater Poland region, which differed in soil properties and moisture. Leaf blades from the middle level of Melica nutans generative shoots were used as analytical material to measure the content of chlorophyll a, chlorophyll b, β-carotene and total carotenoids. The average (±SD) content of chlorophyll a in the Melica nutans plants growing in the less fertile site (Location I) amounted to 6.67 ± 0.99 mg·g−1 DM. The average content of chlorophyll b in the same plants was 2.44 ± 0.39 mg·g−1 DM. The Melica nutans plants growing in the more fertile site (Location II) had a higher content of chlorophyll a, i.e., 7.76 ± 0.96 mg·g−1 DM, and lower content of chlorophyll b, i.e., 2.36 ± 0.26 mg·g−1 DM. The plants collected from both sites had similar content of β-carotene, i.e., 0.61 ± 0.11 mg·g−1 DM Location I and 0.62 ± 0.07 mg·g−1 DM Location II. The plants growing in the less fertile site (Location I) had significantly higher total carotene content than the plants in the more fertile site (Location II). The content of pigments in the Melica nutans plants was significantly differentiated by the meteorological conditions in the subsequent years of the research. In the first year of the research, the average content of all chlorophyll pigments in the plants was significantly higher than in the second drier year, regardless of the site.


2019 ◽  
Vol 6 (01) ◽  
pp. 30-40
Author(s):  
Okky Tiara Sari Dewi ◽  
Muhamad Syukur

The consumption of yard long bean in Indonesia is high and it has been increasing continuously, but the production and harvest area has been decreasing. New, superior long bean varieties with higher productivity are required to meet the increasing demands. The aim of this study was to evaluate the morphological and yield characters of selected purple and green long bean genotypes. The research was conducted from February to May 2018 in the Madiun district, East Java, Indonesia. The experiment was laid out in a randomized complete block design with genotypes as the single factor treatment, replicated three times. Eleven yard long bean genotypes were tested, consisting of four new genotypes, F7-013014- 4U-16-1-1, “F7-013014-4U-16-1-2”, “F7-013014- 4U-16-1-3”, and “F7-013014-7P-4-1-1”, and seven control genotypes, “KP13”, “KP14”, “KP Putih China”, “KP Putih China”, “Borneo”, “Sabrina” and “Parade”. The new genotypes, “F7-013014-4U-16-1-1, “F7- 013014-4U-16-1-2”, “F7-013014-4U-16-1-3”, and “F7-013014-7P-4-1-1” had at least one superior characters compared to the control genotypes, i.e. earlier fl owering and time to harvest, longer leaves, greater pod weight, longer pods, larger pod diameter, and higher contents of anthocyanin, carotene, chlorophyll a, chlorophyll b, and total chlorophyll. Keywords: anthocyanin, genotype, pigment, carotene, chlorophyll


2020 ◽  
Vol 2 (1) ◽  
pp. 14-20
Author(s):  
Musa Tanko Muhammad ◽  
Jabo Abdullahi Dalhatu ◽  
Adamu Abdullahi ◽  
Murtala Sani Yakubu ◽  
Ibrahim Ibafidon Madinat

A survey was conducted to assess the flora affected by mistletoe species and the identification of chlorophyll pigments in Sokoto State University. The study was aimed at surveying the flora affected by mistletoe species and identifies their chlorophyll pigments within the Sokoto State University campus. The study site was divided into three sampling areas, based on the studied species and infestation of trees by mistletoes. The species of mistletoe, Tapinanthus globiferus, was common to all the three types of trees (Azadirachta indica, Psidium guajava, and Acacia nilotica). A. nilotica had the highest rate of infestation by mistletoe (T. globiferus) with the percentage of 85.7% in area A, 91.7% in area B, and 82.3% in area C. The estimation of pigment content of chlorophyll in mistletoe leaves showed that all the mistletoe found on the three different types of trees had the same pigment content of chlorophyll-a, chlorophyll-b, and carotene.


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