scholarly journals Ultrafiltration as a simple purification method of a water extract of common bean seed as a natural coagulant

2020 ◽  
Vol 74 (3) ◽  
pp. 211-220
Author(s):  
Jelena Prodanovic ◽  
Bojana Saric ◽  
Marina Sciban ◽  
Dragana Kukic ◽  
Vesna Vasic ◽  
...  
Keyword(s):  
Organic Matter ◽  
Common Bean ◽  
Crude Extract ◽  
Water Extract ◽  
Bean Seed ◽  
Coagulation Activity ◽  
Turbidity Removal ◽  
Molecular Weights ◽  
Natural Coagulants ◽  
Model Water

Natural coagulants from a crude water extract of common bean seed showed very good efficiency of turbidity removal from water of ~89 % under optimal coagulation conditions, which were determined using response surface methodology (RSM). However, they also increased the content of organic matter in treated model water by ~66 %, which is the main drawback of usage of natural coagulants, in general. Thus, ultrafiltration was applied for processing of the crude water extract in order to separate biomolecules, which exhibit the coagulation activity. Four fractions obtained by ultrafiltration were applied in coagulation tests under the same conditions as the crude extract, and the 4th fraction (molecules with molecular weights >30 kDa) with the predominant content of proteins with molecular weights 50 - 60 kDa, achieved almost as high efficiency of turbidity removal (75 %) as the crude extract. At the same time, the content of organic matter in treated water increased just for 16 % in comparison to the blank (model water processed in the same way but without coagulant). After optimization of process parameters by RSM for usage of the 4th fraction, the coagulation activity increased further to 80 %.

scholarly journals Treatment of sugar beet thick juice spent wash by chemical and natural coagulants

2009 ◽  
pp. 177-182
Author(s):  
Marina Sciban ◽  
Mile Klasnja ◽  
Mirjana Antov
Keyword(s):  
Sugar Beet ◽  
Common Bean ◽  
Bioethanol Production ◽  
Bean Seed ◽  
Coagulation Activity ◽  
Turbidity Removal ◽  
Natural Coagulant ◽  
Aluminium Sulfate ◽  
Natural Coagulants ◽  
Thick Juice

The possibility of treatment of wastewater from bioethanol production by aluminium sulfate and natural coagulant extracted from common bean seed was studied. The highest coagulation activity at pH 6.5 is reached with analum dose of 1 g/l, but only a little lower coagulation activities were obtained by the dose of 0.05 and 0.10 g/l, which is more favorable for economic and environmental reasons. When natural coagulant from common bean was applied the highest coagulation activity, 14.3%, at pH 6.5 is reached with a dose of 0.5 ml/l. However, when common bean natural coagulant was used simultaneously with alum, the highest turbidity removal resulting in 24% coagulation activity was achieved and this was more efficient than when alum or natural coagulant were used.

scholarly journals Investigation of isolation conditions and ion-exchange purification of protein coagulation components from common bean seed

2007 ◽  
pp. 3-10
Author(s):  
Mirjana Antov ◽  
Marina Sciban ◽  
Slavica Adamovic ◽  
Mile Klasnja
Keyword(s):  
Ion Exchange ◽  
Common Bean ◽  
Crude Extract ◽  
Protein Concentration ◽  
Extraction Procedure ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Bean Seed ◽  
Coagulation Activity ◽  
Water As Solvent

Investigation of an extraction procedure of protein coagulants from common bean seed regarding concentration of NaCl and pH was performed. High values of protein concentration and coagulation activity in crude extract (9.19 g/l and 23.9%, respectively) were obtained when the extraction was performed using 0.5 mol/l NaCl and water as solvent, which represents an advantage for economic and environmental reasons. Crude extract of common bean seed was purified by precipitation at two different percentages of (NH4)2SO4 saturation, followed by batch ion-exchange chromatography. The highest obtained coagulation activity, 45%, was determined in fraction that was eluated at 1.75 mol/l NaCl from resin loaded with proteins precipitated upon 80-100% (NH4)2SO4 saturation. High values of coagulation activity showed by some eluates suggest their application as natural coagulant for water purification. .

scholarly journals Extraction and partial purification of coagulation active components from common bean seed

2006 ◽  
pp. 37-43 ◽  
Author(s):  
Marina Sciban ◽  
Mirjana Antov ◽  
Mile Klasnja
Keyword(s):  
Common Bean ◽  
Anion Exchange ◽  
Crude Extract ◽  
Partial Purification ◽  
Turbid Water ◽  
Nacl Solution ◽  
Bean Seed ◽  
Active Components ◽  
Coagulation Activity ◽  
Nacl Concentration

An active coagulation component was extracted from common bean seed by NaCl solution and the obtained crude extract was partially purified through a sequence of steps that included precipitation of protein by ammonium sulphate, desalting by dialysis and anion exchange. A turbid water was treated by protein fractions obtained in the anion- exchange elution process by stepwise increase in NaCl concentration. The jar tests were conducted at various dosages of eluates. Different mode of relation between coagulation activity and applied coagulant dose for each protein fraction indicated the existence of different mechanisms of coagulation/flocculation, depending of characteristics of different proteins in the fractions.

scholarly journals The purification of natural coagulant extracted from common bean on IRA 958 Cl anion exchange resin

2020 ◽  
pp. 31-31
Author(s):  
Jelena Prodanovic ◽  
Marina Sciban ◽  
Dragana Kukic ◽  
Vesna Vasic ◽  
Nevena Blagojev ◽  
...  
Keyword(s):  
Common Bean ◽  
Anion Exchange ◽  
Crude Extract ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Batch Process ◽  
Organic Load ◽  
Coagulation Activity ◽  
Natural Coagulant ◽  
Treated Water

Natural coagulants are of organic nature and can increase organic load of treated water, thus they require purification in order to remove compounds that do not have coagulation activity. In this work natural coagulant was extracted from 50 g L-1 of ground common bean with 0.5 mol L-1 NaCl. Proteins from this crude extract were precipitated by adding ammonium-sulphate. After precipitation, separation and resolution of proteins, further purification was done by anion-exchange resin Amberlite IRA 958 Cl in batch process. Partially purified coagulant eluted by 2 mol L-1 NaCl solution achieved the highest coagulation activity of 53.3 % at dose of 1 mL L-1 although it contained the lowest amount of proteins, but just slightly lower coagulation activity of 49.8 % was achieved at more than 5 times lower dosage of the same fraction. Organic load in treated water when purified fraction was applied as coagulant was almost 4 times lower than in case of crude extract as coagulant.

scholarly journals Coagulation Activity of the Seed Extract from Zygia cauliflora (WILLD.) KILLIP applied in Water Treatment

2020 ◽  
Vol 15 (6) ◽  
pp. 1
Author(s):  
Julia Aoki Domingues ◽  
Nelson Consolin Filho ◽  
Luiz Augusto Gomes de Souza ◽  
Flávia Vieira da Silva Medeiros
Keyword(s):  
Aqueous Solution ◽  
Water Treatment ◽  
Saline Solution ◽  
Seed Extract ◽  
Coagulation Activity ◽  
Turbidity Removal ◽  
Promising Alternative ◽  
Coagulation Mechanism ◽  
Natural Coagulants ◽  
Saline Solutions

The use of natural coagulants is a promising alternative to replace or assist chemical coagulants due to its numerous advantages. This paper evaluates the effectiveness of a natural coagulant in water treatment. The coagulant was extracted from the Zygia cauliflora (Willd.) Killip seed in saline solutions and defatted using 95% ethanol. The tests were conducted using different concentrations of the coagulant (0.1, 0.5, 1.0, 2.0, 3.0, and 4.0 g L-1) and of NaCl (0.0 M, 1.0 M, and 5.0 M). The results showed that the use of 2 g L-1 of coagulant and 1.0 M of the salt achieved an effectiveness of 20% and 70%, respectively, of color and turbidity removal. The analysis showed an effectiveness of 70% and 30%, respectively, when removing the UV254 compound and DOM (dissolved organic matter). The mechanism for turbidity removal by the coagulants prepared with aqueous solution and with saline solution at 1.0 M of NaCl is supposed to be adsorption and charge neutralization, respectively, following the Freundlich and Langmuir models. However, the coagulant prepared with saline solution at 5.0 M of NaCl tends to form a netlike structure followed by turbidity removal through a sweep coagulation mechanism. Future papers should therefore focus on the use of Zygia cauliflora (Willd.) Killip as an alternative for replacement or use as an auxiliary chemical coagulant.

scholarly journals Influence of the composition of common bean extracts on their coagulation ability

2011 ◽  
pp. 71-79 ◽  
Author(s):  
Dragana Kukic ◽  
Marina Sciban ◽  
Aleksandra Tepic ◽  
Jelena Prodanovic
Keyword(s):  
Chemical Composition ◽  
Common Bean ◽  
Phytic Acid ◽  
Total Phenolics ◽  
Distilled Water ◽  
Coagulation Activity ◽  
Total Sugars ◽  
Coagulation And Flocculation ◽  
Natural Coagulants ◽  
Phenolics Content

Coagulation and flocculation are the most used methods for removal of turbidity of water. Recently, many studies have focused on the investigation of natural coagulants for this purpose. In view of the fact that extracts of common bean have coagulation activity, this study is concerned with the chemical composition of these extracts and their influence on the coagulation activity. Extraction was conducted with distilled water, 0.5M NaCl and 1M NaCl and total sugars content, proteins, phytic acid and total phenolics content and their coagulation activity were determined in the obtained extracts. These experiments confirmed that an extraction time of 10 minutes is sufficient for the extraction of active coagulant components from common bean seeds and that water is satisfactorily efficient and most economical solvent.

THE EXTRACTION OF ORGANIC MATTER FROM SELECTED SOILS AND PARTICLE SIZE FRACTIONS WITH 0.5 M NaOH AND 0.1 M Na4P2O7 SOLUTIONS

1989 ◽  
Vol 69 (2) ◽  
pp. 253-262 ◽  
Author(s):  
M. SCHNITZER ◽  
P. SCHUPPLI
Keyword(s):  
Molecular Weight ◽  
Organic Matter ◽  
Humic Acids ◽  
Nmr Spectra ◽  
Attractive Alternative ◽  
Particle Size Fractions ◽  
Molecular Weights ◽  
Naoh Solution ◽  
C Nmr ◽  
Medium Silt

Organic matter (OM) in the Bainsville and Melfort soils, and in coarse clay and medium silt fractions separated from these soils, was extracted under N2 with 0.5 M NaOH and unadjusted 0.1 M Na4P2O7 solutions. pH ranges of the soils and fractions in contact for 24 h with 0.5 M NaOH and 0.1 M Na4P2O7 solutions extended from 12.2 to 12.6 and 9.0 to 9.4, respectively. Slightly greater proportions of the soil-carbon were extracted by 0.5 M NaOH than by 0.1 M Na4P2O7 solution. The differences, however, did not appear to be significant and may vary from soil to soil. The efficiency of extraction and the characteristics of the extracted materials were assessed on humic acids (HAs), which were isolated from the extracts. From the Bainsville soil and fractions, 0.1 M Na4P2O7 solution extracted more high-molecular weight and more deeply colored HAs than did 0.5 M NaOH solution. But HAs extracted from the Melfort soil and fractions had similar molecular weights and colours. 13C NMR spectra showed that HAs extracted by 0.1 M Na4P2O7 solution tended to be more aromatic than HAs extracted by 0.5 M NaOH solution. Well defined solid-state 13C NMR spectra of HAs, containing up to 69.0% ash, could be recorded. Unadjusted 0.1 M Na4P2O7 solution under N2 was found to be an attractive alternative to 0.5 M NaOH solution as an extractant for soil OM. Key words: Humic acids, E4:E6 ratios, IR spectra, 13C NMR spectra, aromaticity

scholarly journals Karakterisasi Ekstrak Tapioka dan Tapioka Ionik sebagai Biokoagulan dalam Proses Pengolahan Air

2018 ◽  
Vol 19 (2) ◽  
pp. 165
Author(s):  
Eka Prihatinningtyas ◽  
Agus Jatnika Effendi
Keyword(s):  
Ion Exchange ◽  
Isoelectric Point ◽  
Ftir Analysis ◽  
Active Components ◽  
Turbidity Removal ◽  
Natural Coagulant ◽  
Coagulation Process ◽  
Or Groups ◽  
Natural Coagulants ◽  
Artificial Water

ABSTRACTThe ability of tapioca to act as natural coagulants (biocoagulants) was tested using artificial water. As turbidity was added as kaolin. This research aimed to determine the compounds and or groups that act as natural coagulant and to describe the mechanism of flocculation: extraction which yields tapioca extract and ion exchange which yields ionic tapioca. Coagulation process was performed at three different initial turbidities, i.e. 50 NTU (low turbidities), 150 NTU (middle turbidities) and 300 NTU (high initial turbidites). At the same condition (coagulant dose 20 ppmv, pH 5), ionic tapioca yield better turbidity removal compared tapioca extract i.e 11.2% at low initial turbidites; 2.4% at middle initial turbidities and 12.8% at high initial turbidities. FTIR analysis  showed that tapioca extract and ionic tapioca contained of carboxyl, hydroxyl and amides groups which  can act as active components on coagulation process. The presence of those groups caused positive and negative charges (amphoter). Coagulation process ran efficiently at pH 5 because the isoelectric point is obtained at that condition.Keyword : bio coagulants, coagulation,  coagulant agents, ionic tapioca, tapioca extract,ABSTRAK Kemampuan tepung tapioka sebagai koagulan alami (biokoagulan) telah diuji dengan menggunakan limbah artifisial dari kaolin. Penelitian ini bertujuan untuk menentukan senyawa atau gugus yang berperan sebagai biokoagulan dan menjelaskan mekanisme flokulasi yang terjadi. Perlakuan awal tapioca sebelum digunakan sebagai koagulan adalah ekstraksi yang menghasilkan ekstrak tapioka  dan pertukaran ion  yang menghasilkan tapioka ionik. Proses koagulasi dilakukan pada 3 macam kekeruhan awal yaitu 50 NTU (kekeruhan rendah), 150 NTU (kekeruhan sedang) dan 300 NTU (kekeruhan tinggi). Pada kondisi operasi yang sama (dosis 20 ppmv dan pH 5), tapioka ionik memberikan efisiensi penurunan kekeruhan yang lebih tinggi, yaitu sebesar 11,0% pada kekeruhan awal 50 NTU; 2,4% pada kekeruhan awal 150 NTU dan 12,8% pada kekeruhan awal 300 NTU. Hasil analisa FTIR menunjukkan bahwa ekstrak tapioka dan tapioka ionik mempunyai gugus karboksil (-OH), gugus karboksil (-COOH) dan gugus amida (-CONH2). Keberadaan ketiga gugus tersebut menyebabkan biokoagulan ini memiliki muatan positif dan negatif sekaligus (amfoter). Proses koagulasi berjalan dengan efisien pada pH 5 karena titik isoelektrik diperoleh pada pH tersebut. Kata kunci : biokoagulan, koagulasi, agen koagulan, ekstrak tapioka, tapioka ionik

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