scholarly journals Influence of the composition of common bean extracts on their coagulation ability

2011 ◽  
pp. 71-79 ◽  
Author(s):  
Dragana Kukic ◽  
Marina Sciban ◽  
Aleksandra Tepic ◽  
Jelena Prodanovic

Coagulation and flocculation are the most used methods for removal of turbidity of water. Recently, many studies have focused on the investigation of natural coagulants for this purpose. In view of the fact that extracts of common bean have coagulation activity, this study is concerned with the chemical composition of these extracts and their influence on the coagulation activity. Extraction was conducted with distilled water, 0.5M NaCl and 1M NaCl and total sugars content, proteins, phytic acid and total phenolics content and their coagulation activity were determined in the obtained extracts. These experiments confirmed that an extraction time of 10 minutes is sufficient for the extraction of active coagulant components from common bean seeds and that water is satisfactorily efficient and most economical solvent.

Author(s):  
Ade Chandra Iwansyah ◽  
Rizal M. Damanik ◽  
Lilik Kustiyah ◽  
Muhammad Hanafi

<p><strong>Objective: </strong>The objectives of this research were to evaluate and compare the antioxidant activity; phenolics and flavonoids contents; and chemical composition of some galactopoietic herbs used in Indonesia and to find a possible relationship between antioxidant activity; phenolics, flavonoids and chemical composition of these plants.</p><p><strong>Methods: </strong>A wide range of analytical parameters was studied, including yield; pH; water activity (a<sub>w</sub>); total color difference; phenolics and flavonoids content; moisture; ash; protein; fat; carbohydrate; fiber; calcium; zinc; magnesium; potassium; iron content; and the data were analyzed by partial least squares (PLS) regression analysis and principal component analysis (PCA) to evaluate the correlation between the parameters and classification of the plants. Folin–cioceltaeu assay, aluminum chloride assay and DPPH scavenging assay were used to evaluate of total phenolics content and free radical scavenging activity of the ethanolic extract of galactopoietic herbs.</p><p><strong>Results: </strong>All of the galactagogue herbs, <em>viz</em>. <em>Plectranthus amboinicus </em>(Lour.) (PA), <em>Sauropus androgynus</em> (SA), <em>Moringa oleifera</em> (MO), and <em>Carica papaya</em> leaves (CP) exhibited good results of TPC, TFC and antioxidant activity. MO contained the highest average of total phenolics content (53.89 mg GAE/g). SA with a ratio of total flavonoids to total phenolics (TF/TP) which is 1.59 contained the highest average of total flavonoids and antioxidant activity (IC<sub>50</sub>), 43.63 mg QE/g and 126.13 μg/ml, respectively, followed by MO&gt;CA&gt;PA. Based on PLS and PCA analysis, antioxidant activity correlated with fat, protein, phenolics content, flavonoids content and pH, but only the total flavonoids content correlated strongly with antioxidant activity.<strong></strong></p><p><strong>Conclusion: </strong>This provides useful data about the studied relationships and chemical patterns in some galactopoietic herbs used and provides a helpful tool for understanding much more about the interpretation of results from the experiments and the mechanism of biosynthesis in plants.</p>


2020 ◽  
Vol 74 (3) ◽  
pp. 211-220
Author(s):  
Jelena Prodanovic ◽  
Bojana Saric ◽  
Marina Sciban ◽  
Dragana Kukic ◽  
Vesna Vasic ◽  
...  

Natural coagulants from a crude water extract of common bean seed showed very good efficiency of turbidity removal from water of ~89 % under optimal coagulation conditions, which were determined using response surface methodology (RSM). However, they also increased the content of organic matter in treated model water by ~66 %, which is the main drawback of usage of natural coagulants, in general. Thus, ultrafiltration was applied for processing of the crude water extract in order to separate biomolecules, which exhibit the coagulation activity. Four fractions obtained by ultrafiltration were applied in coagulation tests under the same conditions as the crude extract, and the 4th fraction (molecules with molecular weights >30 kDa) with the predominant content of proteins with molecular weights 50 - 60 kDa, achieved almost as high efficiency of turbidity removal (75 %) as the crude extract. At the same time, the content of organic matter in treated water increased just for 16 % in comparison to the blank (model water processed in the same way but without coagulant). After optimization of process parameters by RSM for usage of the 4th fraction, the coagulation activity increased further to 80 %.


2009 ◽  
pp. 177-182
Author(s):  
Marina Sciban ◽  
Mile Klasnja ◽  
Mirjana Antov

The possibility of treatment of wastewater from bioethanol production by aluminium sulfate and natural coagulant extracted from common bean seed was studied. The highest coagulation activity at pH 6.5 is reached with analum dose of 1 g/l, but only a little lower coagulation activities were obtained by the dose of 0.05 and 0.10 g/l, which is more favorable for economic and environmental reasons. When natural coagulant from common bean was applied the highest coagulation activity, 14.3%, at pH 6.5 is reached with a dose of 0.5 ml/l. However, when common bean natural coagulant was used simultaneously with alum, the highest turbidity removal resulting in 24% coagulation activity was achieved and this was more efficient than when alum or natural coagulant were used.


2005 ◽  
pp. 81-90 ◽  
Author(s):  
Marina Sciban ◽  
Mile Klasnja ◽  
Jelena Stojimirovic

The ability of seeds of plants: Phaseolus vulgaris, Robinia pseudoacacia Ceratonia siliqua and Amorpha fruticosa, to act as natural coagulants was tested using synthetic turbid water. This water was prepared by adding kaolin into tap water, just before the test. Active components were extracted from ground seeds with distilled water. The coagulation ability of this extract was assessed by the use of standard jar test measurements in water with various initial turbidity. Investigation of these natural coagulants was confirmed their positive coagulation activity. Of all plants that have been examined, the seed extract from Ceratonia siliqua appeared to be one of the most effective coagulants for water treatment. A dose of 20 mg/l of this coagulant resulted in 100% coagulation activity for clarification of water with 17.5 NTU initial turbidity.


2007 ◽  
Vol 67 (1) ◽  
pp. 149-161 ◽  
Author(s):  
Marek Gajewski ◽  
Paweł Szymczak ◽  
Krystyna Elkner ◽  
Aleksandra Dąbrowska ◽  
Anna Kret ◽  
...  

Some Aspects of Nutritive and Biological Value of Carrot Cultivars with Orange, Yellow and Purple-Coloured RootsThe aim of this work was to determine antioxidant activity and some physical and chemical parameters influencing nutritive and biological value of orange, purple and yellow carrot cultivars. The two-year experiment was carried out in Warsaw Agricultural University in 2005 and 2006. Carrot was grown in the experimental field of the University. Six carrot cultivars, differed in storage roots colour and shape, were chosen for the experiment: 'Florida' F1, 'Interceptor' F1, 'Nebula' F1, 'Purple Haze' F1, 'Yellowstone', 'Mello Yello' F1. Immediately after harvest of carrots there were determined: dry matter, total sugars content, total phenolics, total carotenoids, antioxidant activity (DPPH), fractions of dietary fibre, pectines, pH, redox potential, electrical resistance. P-value, which is a combined parameter of some physical and chemical traits, was also calculated. Correlations between some of these quality parameters were determined. Dry mater content was the highest for 'Purple Haze' in 2005, but in 2006 for 'Nebula' similar dry matter content was found. Total sugars content in carrots in 2005 was the highest in 'Purple Haze' but in 2006 'Florida' showed higher sugars content. Total phenolics content was the highest in 'Purple Haze' roots. Total carotenoids content was greatly differentiated between cultivars and varied from below 1 mg·100 g-1to above 14 mg·100 g-1. For cultivars of yellow storage roots the lowest level of carotenoids were found, and the highest for orange-coloured 'Florida', 'Interceptor' and 'Purple Haze'. In all cultivars, from fractions of total fibre, the highest amount was found in the case of cellulose and pectines. The highest antioxidant activity showed purple-coloured cultivar 'Purple Haze', and the lowest activity yellow-coloured cultivars 'Yellowstone' and 'Mello Yello'. Regression analysis showed that antioxidant activity of carrot storage roots was strictly related to carotenoids content (r=0.92) and phenolics content (r=0.87).


2010 ◽  
pp. 141-147 ◽  
Author(s):  
Marina Sciban ◽  
Mirjana Vasic ◽  
Jelena Prodanovic ◽  
Mirjana Antov ◽  
Mile Klasnja

Coagulation and flocculation by adding chemicals are the methods that are usually used for removal of water turbidity. This study is concerned with the coagulation activity of extracts of various strains of bean. The aim was to ascertain if bean varieties influence coagulation activity. Active components were extracted from 1 g of ground sample with 100 ml distilled water. Contents of dry matter and nitrogen were specified in the solid samples, and the content of soluble nitrogen was determined in the extracts. These data were used to calculate the efficiency of extraction of nitrogen-containing compounds. The coagulation activity was assessed by jar test using synthetic turbid water, of the initial pH 9 and turbidity 35 NTU. The jar test was carried out by adding different amounts of extracts to model water, and stirring the content. After sedimentation for 1 h, residual turbidity was determined by turbidimeter and coagulation activity was calculated. The increment of organic matter concentration after the coagulation was also determined. These experiments confirmed that extracts of all investigated strains of bean could be used successfully as natural coagulants.


2021 ◽  
Vol 11 (9) ◽  
pp. 4003
Author(s):  
Sílvia Petronilho ◽  
Alisa Rudnitskaya ◽  
Manuel A. Coimbra ◽  
Sílvia M. Rocha

The evaluation of the variety suitability regarding each appellation’s specificities should be a strategy for maximizing the varieties’ oenological potential while contributing to the sustainable production of quality wines, keeping their typicity and rationalizing winemaking costs. Thus, the combination of several grape physicochemical attributes, modulated by climate and vineyard characteristics, providing knowledge for each grape variety’s oenological potential, is a relevant and reliable support for winemakers’ decisions. To prove this hypothesis, six mature grape varieties from three harvests, each one from three vineyard parcels with different topographical conditions from Bairrada Appellation (Portugal), were studied using analysis of variance–simultaneous components analysis (ASCA). The effects of harvest year and parcel on grape berry weight, pH, titratable acidity, total sugars, total phenolics, antiradical activity, and volatile composition in free and glycosidically-linked forms were analyzed. The compositional plasticity of autochthonous varieties (white Arinto and Bical and red Baga, Castelão, and Touriga Nacional) was observed. Sauvignon Blanc grape composition was significantly modulated by harvest. This study represents an important contribution for the maintenance of varieties’ biodiversity while contributing to establishing their peculiarities. Autochthonous varieties, if accurately exploited, can provide higher characteristic diversity than worldwide used varieties, an aspect to be more objectively taken into consideration by winemakers.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Song Yu ◽  
Xuetian Zhu ◽  
Helin Yang ◽  
Lihe Yu ◽  
Yifei Zhang

AbstractSeed deterioration, coupled with a decrease in nutrients, is unavoidable following long-term storage, and these seeds are therefore used as livestock fodder. Here, we developed a simple, rapid and efficient method of producing high amounts of antioxidants from deteriorated seeds via melatonin-induced germination. Legume seeds were subjected to high humidity at 55 °C for 12–36 h to obtain aged seeds with a 40% germination rate and severely reduced antioxidant nutrition (total phenolics content, ferric reducing power and 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging capacity). Aged seeds were then treated with 0.1 mM melatonin, resulting in the production of sprouts with a higher total phenolics content (fivefold), greater ferric reducing power (sevenfold) and greater DPPH radical scavenging capacity (twofold) compared to the aged seeds. These findings suggest that melatonin treatment efficiently converted aged seed reserve residues into antioxidant nutrients, providing an alternative use for deteriorated seeds in food production.


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