scholarly journals Extraction and partial purification of coagulation active components from common bean seed

2006 ◽  
pp. 37-43 ◽  
Author(s):  
Marina Sciban ◽  
Mirjana Antov ◽  
Mile Klasnja
Keyword(s):  
Common Bean ◽  
Anion Exchange ◽  
Crude Extract ◽  
Partial Purification ◽  
Turbid Water ◽  
Nacl Solution ◽  
Bean Seed ◽  
Active Components ◽  
Coagulation Activity ◽  
Nacl Concentration

An active coagulation component was extracted from common bean seed by NaCl solution and the obtained crude extract was partially purified through a sequence of steps that included precipitation of protein by ammonium sulphate, desalting by dialysis and anion exchange. A turbid water was treated by protein fractions obtained in the anion- exchange elution process by stepwise increase in NaCl concentration. The jar tests were conducted at various dosages of eluates. Different mode of relation between coagulation activity and applied coagulant dose for each protein fraction indicated the existence of different mechanisms of coagulation/flocculation, depending of characteristics of different proteins in the fractions.

scholarly journals Ultrafiltration as a simple purification method of a water extract of common bean seed as a natural coagulant

2020 ◽  
Vol 74 (3) ◽  
pp. 211-220
Author(s):  
Jelena Prodanovic ◽  
Bojana Saric ◽  
Marina Sciban ◽  
Dragana Kukic ◽  
Vesna Vasic ◽  
...  
Keyword(s):  
Organic Matter ◽  
Common Bean ◽  
Crude Extract ◽  
Water Extract ◽  
Bean Seed ◽  
Coagulation Activity ◽  
Turbidity Removal ◽  
Molecular Weights ◽  
Natural Coagulants ◽  
Model Water

Natural coagulants from a crude water extract of common bean seed showed very good efficiency of turbidity removal from water of ~89 % under optimal coagulation conditions, which were determined using response surface methodology (RSM). However, they also increased the content of organic matter in treated model water by ~66 %, which is the main drawback of usage of natural coagulants, in general. Thus, ultrafiltration was applied for processing of the crude water extract in order to separate biomolecules, which exhibit the coagulation activity. Four fractions obtained by ultrafiltration were applied in coagulation tests under the same conditions as the crude extract, and the 4th fraction (molecules with molecular weights >30 kDa) with the predominant content of proteins with molecular weights 50 - 60 kDa, achieved almost as high efficiency of turbidity removal (75 %) as the crude extract. At the same time, the content of organic matter in treated water increased just for 16 % in comparison to the blank (model water processed in the same way but without coagulant). After optimization of process parameters by RSM for usage of the 4th fraction, the coagulation activity increased further to 80 %.

scholarly journals The purification of natural coagulant extracted from common bean on IRA 958 Cl anion exchange resin

2020 ◽  
pp. 31-31
Author(s):  
Jelena Prodanovic ◽  
Marina Sciban ◽  
Dragana Kukic ◽  
Vesna Vasic ◽  
Nevena Blagojev ◽  
...  
Keyword(s):  
Common Bean ◽  
Anion Exchange ◽  
Crude Extract ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Batch Process ◽  
Organic Load ◽  
Coagulation Activity ◽  
Natural Coagulant ◽  
Treated Water

Natural coagulants are of organic nature and can increase organic load of treated water, thus they require purification in order to remove compounds that do not have coagulation activity. In this work natural coagulant was extracted from 50 g L-1 of ground common bean with 0.5 mol L-1 NaCl. Proteins from this crude extract were precipitated by adding ammonium-sulphate. After precipitation, separation and resolution of proteins, further purification was done by anion-exchange resin Amberlite IRA 958 Cl in batch process. Partially purified coagulant eluted by 2 mol L-1 NaCl solution achieved the highest coagulation activity of 53.3 % at dose of 1 mL L-1 although it contained the lowest amount of proteins, but just slightly lower coagulation activity of 49.8 % was achieved at more than 5 times lower dosage of the same fraction. Organic load in treated water when purified fraction was applied as coagulant was almost 4 times lower than in case of crude extract as coagulant.

scholarly journals Investigation of isolation conditions and ion-exchange purification of protein coagulation components from common bean seed

2007 ◽  
pp. 3-10
Author(s):  
Mirjana Antov ◽  
Marina Sciban ◽  
Slavica Adamovic ◽  
Mile Klasnja
Keyword(s):  
Ion Exchange ◽  
Common Bean ◽  
Crude Extract ◽  
Protein Concentration ◽  
Extraction Procedure ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Bean Seed ◽  
Coagulation Activity ◽  
Water As Solvent

Investigation of an extraction procedure of protein coagulants from common bean seed regarding concentration of NaCl and pH was performed. High values of protein concentration and coagulation activity in crude extract (9.19 g/l and 23.9%, respectively) were obtained when the extraction was performed using 0.5 mol/l NaCl and water as solvent, which represents an advantage for economic and environmental reasons. Crude extract of common bean seed was purified by precipitation at two different percentages of (NH4)2SO4 saturation, followed by batch ion-exchange chromatography. The highest obtained coagulation activity, 45%, was determined in fraction that was eluated at 1.75 mol/l NaCl from resin loaded with proteins precipitated upon 80-100% (NH4)2SO4 saturation. High values of coagulation activity showed by some eluates suggest their application as natural coagulant for water purification. .

scholarly journals Treatment of sugar beet thick juice spent wash by chemical and natural coagulants

2009 ◽  
pp. 177-182
Author(s):  
Marina Sciban ◽  
Mile Klasnja ◽  
Mirjana Antov
Keyword(s):  
Sugar Beet ◽  
Common Bean ◽  
Bioethanol Production ◽  
Bean Seed ◽  
Coagulation Activity ◽  
Turbidity Removal ◽  
Natural Coagulant ◽  
Aluminium Sulfate ◽  
Natural Coagulants ◽  
Thick Juice

The possibility of treatment of wastewater from bioethanol production by aluminium sulfate and natural coagulant extracted from common bean seed was studied. The highest coagulation activity at pH 6.5 is reached with analum dose of 1 g/l, but only a little lower coagulation activities were obtained by the dose of 0.05 and 0.10 g/l, which is more favorable for economic and environmental reasons. When natural coagulant from common bean was applied the highest coagulation activity, 14.3%, at pH 6.5 is reached with a dose of 0.5 ml/l. However, when common bean natural coagulant was used simultaneously with alum, the highest turbidity removal resulting in 24% coagulation activity was achieved and this was more efficient than when alum or natural coagulant were used.

scholarly journals Investigation of coagulation activity of natural coagulants from seeds of different leguminose species

2005 ◽  
pp. 81-90 ◽  
Author(s):  
Marina Sciban ◽  
Mile Klasnja ◽  
Jelena Stojimirovic
Keyword(s):  
Tap Water ◽  
Robinia Pseudoacacia ◽  
Seed Extract ◽  
Distilled Water ◽  
Ceratonia Siliqua ◽  
Turbid Water ◽  
Active Components ◽  
Coagulation Activity ◽  
Jar Test ◽  
Natural Coagulants

The ability of seeds of plants: Phaseolus vulgaris, Robinia pseudoacacia Ceratonia siliqua and Amorpha fruticosa, to act as natural coagulants was tested using synthetic turbid water. This water was prepared by adding kaolin into tap water, just before the test. Active components were extracted from ground seeds with distilled water. The coagulation ability of this extract was assessed by the use of standard jar test measurements in water with various initial turbidity. Investigation of these natural coagulants was confirmed their positive coagulation activity. Of all plants that have been examined, the seed extract from Ceratonia siliqua appeared to be one of the most effective coagulants for water treatment. A dose of 20 mg/l of this coagulant resulted in 100% coagulation activity for clarification of water with 17.5 NTU initial turbidity.

Valorization of the Pharmacological Potential of Phytochemical Compounds Contained in the Crude Extract of the Root of a Plant of Withania frutescens L

2019 ◽  
Author(s):  
A. EL Moussaoui ◽  
F. Jawhari ◽  
K. EL Ouahdani ◽  
I. Es-Safi ◽  
D. Bousta ◽  
...  
Keyword(s):  
Crude Extract ◽  
Control Group ◽  
Root Extract ◽  
Nacl Solution ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Pharmacological Potential

Our present study focuses on the evaluation of the analgesic, anti-inflammatory and healing activity of Withania frutescens L. The anti-inflammatory result has an inhibition percentage of 78.87% ± 7.08 at 450 mg/kg and 75.14% ± 6.39 at 400 mg/kg and 89.75% ± 3.44 for diclofenac (1%). When applied locally, the 10% cream has an inflammation inhibition of 96.87% ± 5.85 and 76.14% ± 7.88 for the 5% cream with 89.87 ± 6.20 of reference (Indomethacin). The abdominal contractions of rats treated with the root extract are significantly lower than those of the control group that received only physiological NaCl solution, with 41.20 ± 2.30 for the extract and 82.20 ± 5.04 for NaCl and 53.40 ± 4.94 for the reference. The healing activity of the studied extract records a percentage of contraction of about 93.20% ± 3.36 (Extract 10%), 84.50% ± 3.84 (Extract 5%), 48.47% ± 2.15 (control) and 81.88 ± 2.24 for the reference.

scholarly journals Active Components from Cassia abbreviata Prevent HIV-1 Entry by Distinct Mechanisms of Action

2021 ◽  
Vol 22 (9) ◽  
pp. 5052
Author(s):  
Yue Zheng ◽  
Xian-Wen Yang ◽  
Dominique Schols ◽  
Mattia Mori ◽  
Bruno Botta ◽  
...  
Keyword(s):  
Crude Extract ◽  
Female Genital Tract ◽  
Binding Activity ◽  
Sub Saharan Africa ◽  
Active Components ◽  
Chemical Structures ◽  
3D Space ◽  
In Silico Study ◽  
Sub Saharan ◽  
Hiv 1

Cassia abbreviata is widely used in Sub-Saharan Africa for treating many diseases, including HIV-1 infection. We have recently described the chemical structures of 28 compounds isolated from an alcoholic crude extract of barks and roots ofC. abbreviata, and showed that six bioactive compounds inhibit HIV-1 infection. In the present study, we demonstrate that the six compounds block HIV-1 entry into cells: oleanolic acid, palmitic acid, taxifolin, piceatannol, guibourtinidol-(4α®8)-epiafzelechin, and a novel compound named as cassiabrevone. We report, for the first time, that guibourtinidol-(4α®8)-epiafzelechin and cassiabrevone inhibit HIV-1 entry (IC50 of 42.47 µM and 30.96 µM, respectively), as well as that piceatannol interacts with cellular membranes. Piceatannol inhibits HIV-1 infection in a dual-chamber assay mimicking the female genital tract, as well as HSV infection, emphasizing its potential as a microbicide. Structure-activity relationships (SAR) showed that pharmacophoric groups of piceatannol are strictly required to inhibit HIV-1 entry. By a ligand-based in silico study, we speculated that piceatannol and norartocarpetin may have a very similar mechanism of action and efficacy because of the highly comparable pharmacophoric and 3D space, while guibourtinidol-(4α®8)-epiafzelechin and cassiabrevone may display a different mechanism. We finally show that cassiabrevone plays a major role of the crude extract of CA by blocking the binding activity of HIV-1 gp120 and CD4.

scholarly journals Efficiency of crude corn extract clean-up on different columns in fumonisins determination

2005 ◽  
pp. 95-102 ◽  
Author(s):  
Biljana Abramovic ◽  
Sandra Jaksic ◽  
Zoran Masic
Keyword(s):  
Liquid Chromatography ◽  
Anion Exchange ◽  
Fluorescence Detection ◽  
Crude Extract ◽  
Reversed Phase ◽  
Lower Price ◽  
Strong Anion Exchange ◽  
Chromatographic Resolution ◽  
Immunoaffinity Columns ◽  
Fumonisins B1 And B2

The efficiencies of different clean-up procedures for crude corn extract from corn samples naturally contaminated by fumonisins B1 and B2 were compared. These procedures precede liquid chromatography determination with fluorescence detection. The efficiencies of immunoaffinity columns (IMA) strong anion exchange columns (SAX), as well as columns with reversed-phase C18 (RP C18) were investigated. No significant differences in the obtained results were found, regardless of the crude extract clea-nup procedure. However, the use of IMA columns for clean-up provided better chromatographic resolution, with the clean-up procedure being the simplest and the fastest. Also, because of the possibility of IMA column regeneration, it is possible to prepare ten samples on one column, so all in all, the lower price of SAX and RP C18 columns is of no great significance.

Effect of Coscinium Fenestratum stem extracts on hypertension in animal model

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Zhe Li ◽  
Hang Meng ◽  
Wei Zhang ◽  
Sophia Ogechi Ekeuku ◽  
Patrick Nwabueze Okechukwu
Keyword(s):  
Crude Extract ◽  
Antioxidant Property ◽  
Frog Heart ◽  
Partial Purification ◽  
Force Of Contraction ◽  
Biochemical Method ◽  
Arterial Blood ◽  
Stem Extract ◽  
Coscinium Fenestratum ◽  
Anaesthetized Rat

This study was done to evaluate the anti-hypertensive effects of stem extract of Coscinium fenestratum (CF). The stems were air dried for 2 days, grounded and then soaked in organic solvent for 2 days. Thereafter the filtered extract was concentrated using rotary evaporator at 450C  to obtain the crude extract. Partial purification was done using silica gel column chromatography to separate the crude extract into five fractions A-E. The effect on hypertension was tested by intravenous injection of each extractinto a normotensive anaesthetized rat andperfused frog heart. The effect Mean Arterial Blood Pressure (MABP), Heart rate (beats per minute) (HR) and force of contraction (FOC) was recorded via the BIOPAC LAB PRO SOFTWARE.  The phytochemical compound present in the extract was identified using a simple colorimetric biochemical method. The extract showed a reduction of theMABP, HR and FOC in the presence of norepinephrine and without norepinephrine. The effect of the extract was more than that of commercial drug atenolol. Colorimetric phytochemistry testing revealed the presence of flavonoids, terpenoids, saponins, tanins, cardiac glycoside and alkaloids. The effect maybe because of the type of bioactive compounds present in the extract which has previously shown antioxidant property.

scholarly journals Common bean seed vigor affecting crop grain yield

2016 ◽  
Vol 38 (4) ◽  
pp. 365-370 ◽  
Author(s):  
Vitor Henrique Vaz Mondo ◽  
Adriano Stephan Nascente ◽  
Manoel Oliveira Cardoso Neto
Keyword(s):  
Grain Yield ◽  
Common Bean ◽  
Block Design ◽  
Seedling Emergence ◽  
Research Work ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Bean Seed ◽  
Crop Establishment ◽  
Randomized Block Design

Abstract: Among the factors that affect seedling emergence and crop establishment are seed germination and vigor, which has an important influence on the establishment of the initial plant population and development, and may affect crop yield. Based on this background the objective of this research work was to evaluate the effects of seed vigor of common bean seed lots and its effects on crop grain yield. The treatments consisted of six seed lots of common bean, which were composed by different combinations of high and low vigor seeds. Seed lots were assessed by germination and vigor tests (first count of germination, electrical conductivity and accelerated aging). A field experiment was conducted in a randomized block design, with plots of six lines of five meters and were evaluated for grain yield at harvest. It was concluded that seed vigor affects common bean grain yield; the use of low vigor seeds results in grain yield reductions of up to 20%.

Sign in / Sign up

Export Citation Format

Share Document