scholarly journals Genetic relationship among introduced lentil germplasm using agronomic traits and ISSR markers

Genetika ◽  
2018 ◽  
Vol 50 (2) ◽  
pp. 575-590
Author(s):  
Sevda Babayeva ◽  
Zeynal Akparov ◽  
Litfer Amirov ◽  
Kamila Shikhaliyeva ◽  
Saida Hasanova ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Agronomic Traits ◽  
Issr Markers ◽  
High Yield ◽  
Grain Legume ◽  
Improvement Program ◽  
Breeding Lines ◽  
Genetic Base ◽  
Inter Simple Sequence Repeats ◽  
New Varieties

Lentil (Lens culinaris Medik.) is an annual, cool-season grain legume playing an important role in human and animal nutrition, as soil fertility maintenance. National lentil improvement program in Azerbaijan is currently focused on extending the genetic base of the lentil collection through the introduction of new breeding lines from ICARDA and involving them into breeding. The present study was aimed to evaluate the performance of lentil collection, mainly comprised of ICARDA-derived breeding lines for yield traits under Azerbaijan condition and assess genetic diversity among them using inter simple sequence repeats (ISSR) markers. Many breeding lines of ICARDA exhibited agro-morphological performance superior to those of the local improved varieties. Our studies confirmed that the genetic base of the studied lentil collection is quite above board. A total of 71 bands were generated using 7 ISSR primers in 47 lentil genotypes, of which 62 were polymorphic. Genetic diversity values varied from 0.61 (UBC 848) to 0.95 (UBC 835), with a mean of 0.81. ISSR dendrogram was able to clearly distinguish all lentil accessions. Clear tendention was observed on clustering of genotypes according to their pedigree or origin with few exeptions. The results obtained from the Principal Coordinate Analysis were consistent with the results of cluster analysis, with minor differences. Breeding lines with high agronomic performance and sufficient genetic distance from this study can be used as appropriate parents to get more heterotic recombinants. This will accelerate the creation of new varieties well adapted to eco-geographic condition of Azerbaijan with stable and high yield.

scholarly journals Distance Only Brings You Closer: Application of ISSR Markers to Analyze Molecular Relationships in Roses (Rosa spp.) - The Symbol of Love

2021 ◽  
Author(s):  
Tanay Joshi ◽  
Santosh Kumar ◽  
Lalit Arya ◽  
Amritbir Riar
Keyword(s):  
Genetic Diversity ◽  
Crop Improvement ◽  
Issr Markers ◽  
Improvement Program ◽  
Promising Tool ◽  
Inter Simple Sequence Repeats ◽  
Upgma Cluster Analysis ◽  
Molecular Relationships ◽  
High Degree ◽  
The Rose

Genetic diversity is inevitable in making any crop improvement program successful. DNA fingerprinting technology to assess the genetic relationship among the selected genotypes for identification and cataloging of different species and cultivars of roses is a promising tool for Rosa genomes. The inter-simple sequence repeats markers (ISSRs) were used to investigate the genetic diversity among twenty-one diverse Rosa genotypes belonging to two different species, Rosa hybrida and R. damascena, and three distinct groups of rose varieties, namely Hybrid Tea, Floribunda, and Damask roses. Twenty-four ISSR primers yielded a total of 280 scorable amplified fragments from 250-1800 bp in length, from which 244 were polymorphic, resulting in an average of 86.4% polymorphism. UPGMA cluster analysis based on Jaccard’s pairwise similarity coefficient values ranged from 0.264 to 0.818, clearly distinguished different species and genotypes, grouping them into three distinct clusters. The results confirmed a high degree of variation in the rose germplasm studied highlighting the potential of improvement in roses for the ornamental and perfume industry.

scholarly journals Morphological Characterization, Variability and Diversity among Vegetable Soybean (Glycine max L.) Genotypes

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 671
Author(s):  
Nagaraju Shilpashree ◽  
Sarojinikunjamma Nirmala Devi ◽  
Dalasanuru Chandregowda Manjunathagowda ◽  
Anjanappa Muddappa ◽  
Shaimaa A. M. Abdelmohsen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Improvement ◽  
Genotypic Variation ◽  
Morphological Characterization ◽  
High Yield ◽  
Vegetable Soybean ◽  
Breeding Lines ◽  
Commercial Cultivation ◽  
Soybean Genotypes ◽  
High Heritability

Vegetable soybean production is dependent on the development of vegetable type varieties that would be achieved by the use of germplasm to evolve new agronomically superior yielding vegetable type with beneficial biochemical traits. This can be accomplished by a better understanding of genetics, which is why the research was conducted to reveal the quantitative genetics of vegetable soybean genotypes. Genetic variability of main morphological traits in vegetable soybean genotypes and their divergence was estimated, as a result of the magnitude of genotypic variation (GV), and phenotypic variation (PV) of traits varied among the genotypes. All traits showed high heritability (h2) associated with high genetic advance percentage mean (GAM). Therefore, these variable traits are potential for genetic improvement of vegetable type soybean. Genetic diversity is the prime need for breeding, and the magnitude of genetic diversity values were maximized among specific genotypes. Eight clusters were found for all genotypes; cluster VIII and cluster I were considered to have the most diversity. Cluster VIII consisted of two genotypes (GM-6 and GM-27), based on the mean outcomes of the high yield attributing traits. Hence, these two (GM-6, GM-27) genotypes can be advanced for commercial cultivation; furthermore, other genotypes can be used as source of breeding lines for genetic improvement of vegetable soybean.

scholarly journals Molecular analysis of genetic diversity in population of Silybum marianum (L.) Gaertn in Egypt

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Marwa Hamouda
Keyword(s):  
Genetic Diversity ◽  
Electrophoretic Pattern ◽  
Issr Markers ◽  
Silybum Marianum ◽  
Inter Simple Sequence Repeats ◽  
Sds Page ◽  
Dna Pattern ◽  
Pharmaceutical Properties ◽  
High Level ◽  
Rapd And Issr

Abstract Background Silybum marianum L. Gaertn is a medicinal plant of unique pharmaceutical properties in the treatment of liver disorders and diabetic nephropathy. Biochemical (SDS-PAGE) and molecular markers such as randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) technologies were used in this work to detect genetic diversity of 14 collections of Silybum marianum population in Egypt. Results The electrophoretic pattern of seed protein gave different molecular weight bands, ranging from 24 to 111 KDa with the presence of unique bands. RAPD results revealed a high level of polymorphism (73.2%) using 12 RAPD primers, but only eight of them gave reproducible polymorphic DNA pattern. Sixteen primers were used in the ISSR method; only ten of them yielded clearly identifiable bands. The percentage of polymorphism is about 80% of the studied samples. Conclusion The obtained data confirmed that SDS-protein, RAPD, and ISSR markers are important tools for genetic analysis for Silybum marianum and recommended to give accurate results.

Phenotypic plasticity of yield and related traits in rainfed durum wheat

2013 ◽  
Vol 152 (6) ◽  
pp. 873-884 ◽  
Author(s):  
R. MOHAMMADI
Keyword(s):  
Phenotypic Plasticity ◽  
Durum Wheat ◽  
Environmental Conditions ◽  
Linear Models ◽  
Agronomic Traits ◽  
Grain Weight ◽  
High Yield ◽  
Yield Improvement ◽  
Modern Cultivar ◽  
Breeding Lines

SUMMARYRainfall and temperature are unpredictable in Mediterranean environments, which results in inconsistent environmental conditions for crop growth and a critical source of uncertainty for farmers and growers. The objectives of the present study were to: (i) quantify and compare the plasticity of durum breeding lines, a modern cultivar and landraces on the basis of yield and agronomic traits and (ii) study associations between plasticity of yield and plasticity of agronomic and phenological traits. Plasticity was quantified using linear models for 11 durum breeding lines, one modern cultivar and two landraces grown in 21 diversified environments. The results showed that the effects due to environment, genotype and genotype×environment (G×E) interaction were significant, which indicates the existence of differences among genotypes for plasticity. Yield ranged from 1939 to 2419 kg/ha across environments and the range of plasticity was 0·66–1·13. The breeding lines and the modern cultivar had higher grain yields compared with the landraces at the same level of plasticity. The landraces with below-average plasticity in yield were characterized as tall in stature and late in heading and maturity, whereas the breeding lines and modern cultivar with above-average plasticity in yield were early in heading and maturity, semi-dwarf and high-yielding, which indicates the success in breeding the materials for unpredictable environmental conditions. In conclusion, yield plasticity was associated with yield improvement and high yield plasticity tends to associate with earliness, shorter plants and low grain weight.

Inter Simple Sequence Repeat (ISSR) markers to assess genetic diversity and relatedness within strawberry genotypes

2008 ◽  
Vol 88 (2) ◽  
pp. 313-322 ◽  
Author(s):  
S. C. Debnath ◽  
S. Khanizadeh ◽  
A. R. Jamieson ◽  
C. Kempler
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Genetic Similarity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Group Method ◽  
Sequence Repeat ◽  
Breeding Lines ◽  
Pair Group ◽  
Simple Sequence

The goal of this study was to determine the level of genetic diversity and relatedness among 16 strawberry (Fragaria H ananassa Duch.) cultivars and 11 breeding lines developed in Canada, using Inter Simple Sequence Repeat (ISSR) markers. Seventeen primers generated 225 polymorphic ISSR-PCR bands. Cluster analysis by the unweighted pair-group method with arithmetic averages (UPGMA) revealed a substantial degree of genetic similarity among the genotypes ranging from 63 to 77% that were in agreement with the principal coordinate (PCO) analysis. Geographical distribution for the place of breeding program explained only 1.4% of total variation as revealed by analysis of molecular variance (AMOVA). The ISSR markers detected a sufficient degree of polymorphism to differentiate among strawberry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in current strawberry breeding programs. Key words: Fragaria × ananassa, DNA fingerprinting, multivariate analysis, breeding, genetic similarity

scholarly journals Different Methods for Detecting Heterotic Groups in Maize

2017 ◽  
Vol 27 (1) ◽  
pp. 15-27
Author(s):  
Mohammed M. El-Rouby Mohammed M. El-Rouby
Keyword(s):  
Agronomic Traits ◽  
Inbred Lines ◽  
Issr Markers ◽  
Pedigree Data ◽  
Heterotic Groups ◽  
Agronomic Character ◽  
Inter Simple Sequence Repeats ◽  
Maize Inbreds ◽  
The Cross ◽  
Simple Sequence

Comparing between different methods for classifying 12 maize inbred lines to heterotic groups was achieved using general and specific combining ability effects; agronomic traits to the maize inbreds per se; and ISSR-markers. The results of line-tester design showed that Giza656 lines had the highest yield for the cross with Gemmiza 1021 tester (7.20 ton per hectare) at Nubaria, while Giza655 lines had the highest yield for the cross with Gemmiza 1021 tester (8.24 ton per hectare) at Gemmiza. Regarding Heterotic specific and General Combining Abilities, the inbred lines were grouped into three groups as their testers were, but groups differed from Nubaria to Gemmiza locations. The classification due to Inter Simple Sequence Repeats markers have agreement with pedigree data more than the HSGCA and agronomic characters, because the HSGCA method affected by testers, lines, environments, and the interactions between all of them, also, agronomic character method need to plant the inbreds and evaluate them at more locations and more seasons .Furthermore, all these cautions will not discard the environmental effects but they will reduce it to the minimum values. On the other hand, molecular marker will keep time and efforts needed to evaluate the inbred lines.

scholarly journals Genetic Diversity of Agronomic Traits in Advanced Breeding Lines of Rice (Oryza Sativa)

2019 ◽  
Vol 11 (30) ◽  
pp. 178-187
Author(s):  
Alireza Nabipour ◽  
Mohammad Norouzi ◽  
◽  
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
Agronomic Traits ◽  
Breeding Lines

scholarly journals Evaluation of genetic diversity of cultivated tea clones (Camellia sinensis (L.) Kuntze) in the eastern black sea coast by inter-simple sequence repeats (ISSRS)

Genetika ◽  
2016 ◽  
Vol 48 (1) ◽  
pp. 87-96 ◽  
Author(s):  
Fatih Beris ◽  
Necla Pehlivan ◽  
Melike Kac ◽  
Ayhan Haznedar ◽  
Fatih Coşkun ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Black Sea ◽  
Issr Markers ◽  
Base Pairs ◽  
Inter Simple Sequence Repeats ◽  
Black Sea Coast ◽  
Geographical Position ◽  
Tea Plants ◽  
Relationship Of ◽  
Sea Coast

Tea is the most globally consumed drink after spring water and an important breeding plant with high economical value in Turkey. In half a century, various kinds of tea cultivars have been bred in Turkey to improve the quality and yield of tea plants. Since tea reproduces sexually, tea fields vary in quality. Thus, determining the genetic diversity and relationship of the plants to support breeding and cultivation is important. In this study we aimed to determine the genetic diversity of tea cultivars breeding in the Eastern Black Sea coast of Turkey and the genetic relationship between them, to verify whether the qualitative morphological designations of the clones are genetically true by the ISSR markers. Herein, the genetic diversity and relationships of 18 Turkish tea cultivars were determined using 15 ISSR markers with sizes ranging from 250 to 3000 base pairs. The similarity indices among these cultivars were between 0.456 and 0.743. Based on cluster analysis using UPGMA, some of tea cultivars originating from the same geographical position were found to be clustered closely. Our data provide valuable information and a useful basis to assist selection and cloning experiments of tea cultivars and also help farmers to find elite parental clones for tea breeding in the Eastern Black Sea coast of Turkey.

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