scholarly journals Different Methods for Detecting Heterotic Groups in Maize

2017 ◽  
Vol 27 (1) ◽  
pp. 15-27
Author(s):  
Mohammed M. El-Rouby Mohammed M. El-Rouby
Keyword(s):  
Agronomic Traits ◽  
Inbred Lines ◽  
Issr Markers ◽  
Pedigree Data ◽  
Heterotic Groups ◽  
Agronomic Character ◽  
Inter Simple Sequence Repeats ◽  
Maize Inbreds ◽  
The Cross ◽  
Simple Sequence

Comparing between different methods for classifying 12 maize inbred lines to heterotic groups was achieved using general and specific combining ability effects; agronomic traits to the maize inbreds per se; and ISSR-markers. The results of line-tester design showed that Giza656 lines had the highest yield for the cross with Gemmiza 1021 tester (7.20 ton per hectare) at Nubaria, while Giza655 lines had the highest yield for the cross with Gemmiza 1021 tester (8.24 ton per hectare) at Gemmiza. Regarding Heterotic specific and General Combining Abilities, the inbred lines were grouped into three groups as their testers were, but groups differed from Nubaria to Gemmiza locations. The classification due to Inter Simple Sequence Repeats markers have agreement with pedigree data more than the HSGCA and agronomic characters, because the HSGCA method affected by testers, lines, environments, and the interactions between all of them, also, agronomic character method need to plant the inbreds and evaluate them at more locations and more seasons .Furthermore, all these cautions will not discard the environmental effects but they will reduce it to the minimum values. On the other hand, molecular marker will keep time and efforts needed to evaluate the inbred lines.

scholarly journals Application of Microsatellite and Inter Simple Sequence Markers for Certifying Trueness to Type of Grafted Ramets and Clustering of Persian Walnut Varieties

2021 ◽  
Author(s):  
Masoomeh Hosseini Nickravesh ◽  
Kourosh Vahdati ◽  
fatemeh amini ◽  
Reza Amiri ◽  
Keith Woeste
Keyword(s):  
Ssr Markers ◽  
Issr Markers ◽  
Principal Coordinate Analysis ◽  
Dna Fingerprint ◽  
Polymorphic Ssrs ◽  
Persian Walnut ◽  
Inter Simple Sequence Repeats ◽  
Ssr Loci ◽  
Ssrs Markers ◽  
Simple Sequence

Abstract The utility of seventeen Microsatellite (SSR) markers and fifteen inter simple sequence repeats (ISSR) markers for the identification of twenty eight ramets of 11 varieties of walnut (Juglans regia) was explored. Thirty nine individual genomes were screened using 61 and 38 scorable fragments from SSR and ISSR markers, respectively. The least polymorphic SSR locus was WGA004 (two alleles) and the most polymorphic (5 alleles) was WGA276. Polymorphism information content values ranged from 0.08 (WGA004) to 0.43 (WGA032) in SSR markers and from 0.11 (AGA (AC)7) to 0.49 (CAC(TGT)5) in ISSR markers, with an average of 0.29 and 0.19, respectively. In most cases, grafted varieties with identical names also had the same microsatellites profile. The principal coordinate analysis and clustering (UPGMA) based on the combined marker set emphasized two failures in grafting or off-types, ramets identified as Serr 4 (S4) and Vina 1 (V1). The presence of two off-type ramets in the walnut research orchard emphasizes the importance of using molecular certification for proving true-to-type of walnut orchards. Using 13 polymorphic SSRs, we tabulated a DNA fingerprint chart of 11 walnut varieties. Except for ‘Chandler’, each cultivar could be distinguished using a combination of only two SSR loci. The 13 SSRs markers evaluated in this study could be used in future to identify clones produced from the varieties.

Characterization of Italian populations ofLoliumspp. resistant and susceptible to diclofop by inter simple sequence repeat

Weed Science ◽  
2004 ◽  
Vol 52 (4) ◽  
pp. 554-563 ◽  
Author(s):  
Giovanni Dinelli ◽  
Alessandra Bonetti ◽  
Ilaria Marotti ◽  
Maurizio Minelli ◽  
Pietro Catizone
Keyword(s):  
Potential Role ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Intergeneric Hybridization ◽  
Banding Patterns ◽  
Inter Simple Sequence Repeats ◽  
Range Of Variation ◽  
Simple Sequence

Three ItalianLoliumweed populations, one susceptible and two resistant to diclofop, were characterized by the technique of inter simple sequence repeats (ISSR). The goal of this study was to taxonomically identify theseLoliumpopulations as well as to evaluate evidence for introgression of ISSR fragments fromFestucaand the potential role of this introgression in the diclofop response. ISSR analysis confirmed the genomic background of the weed populations to be consistent with that ofLolium. However, the great range of variation in ISSR banding patterns highlighted that the three ryegrass accessions are mixed populations made up of individuals resulting presumably from intrageneric and intergeneric hybridization in theLolium–Festucacomplex. TwoFestucagenus-discriminating and 20Festucaspecies-discriminating ISSR markers were screened among all the three ryegrass populations. The resistant Tuscania population carried the highest percentage ofFestucagenome (16.8%) followed by the resistant Roma (13.6%) and susceptible Vetralla (7.6%) populations. On the basis of these data some influence ofFestucagenome in diclofop resistance levels of studied ryegrass populations could be hypothesized.

scholarly journals Characterization of Aegilops kotschyi Boiss. x Triticum aestivum L. hybrid lines

2014 ◽  
Vol 66 (4) ◽  
pp. 109-120 ◽  
Author(s):  
Roman Prażak ◽  
Edyta Paczos-Grzęda
Keyword(s):  
Triticum Aestivum ◽  
Zinc Content ◽  
Triticum Aestivum L ◽  
Issr Markers ◽  
Inter Simple Sequence Repeats ◽  
Iron And Zinc ◽  
Germplasm Analysis ◽  
Hybrid Lines ◽  
Simple Sequence

A study of four F5 and one BC1F1 <em>Aegilops kotschyi </em>Boiss. x <em>Triticum aestivum </em>L. hybrid lines was conducted to determine their quantitative morphological and qualitative features as well as a molecular investigation was carried out. Observations of ten quantitative traits showed that the F5 hybrid lines exhibited intermediate values between <em>Ae. kotschyi </em>Boiss. and <em>T. aestivum </em>L., or had similar traits to one of the parents. These hybrid lines had a significantly lower number and weight of grains per main spike, main spike fertility and 1000-grain weight than <em>T. aestivum </em>L. cv. ‘Rusałka’. The BC1F1 hybrid line was characterized by wheat-like fertility and phenotype. The F5 hybrid lines were characterized by much higher variability of the analysed morphological traits than <em>T. aestivum </em>L. cv. ‘Rusałka’. Grains of the hybrid lines had higher protein and micronutrient (iron and zinc) content than wheat grains. The presence of DNA fragments specific to <em>Ae. kotschyi </em>Boiss. in the genotypes of the hybrid lines was confirmed by seven ISSR (Inter Simple Sequence Repeats) molecular markers. Two ISSR markers – ISSR23690 and ISSR33650 – were the most effective for germplasm analysis of the hybrid lines. The analysed lines can become a source material for improvement of common wheat <em>T. aestivum </em>L. in crossing programs.

Sign in / Sign up

Export Citation Format

Share Document