scholarly journals Protective effect of the antioxidant enzyme on sperm from Bubalus bubalis type after cryoconservation

2011 ◽  
Vol 27 (3) ◽  
pp. 943-949 ◽  
Author(s):  
R. Stefanov ◽  
M. Sabev ◽  
M. Ivanova-Kicheva
Keyword(s):  
Superoxide Dismutase ◽  
Free Radicals ◽  
Protective Effect ◽  
Antioxidant Enzyme ◽  
Water Buffalo ◽  
Test Sample ◽  
Bubalus Bubalis ◽  
Toxic Action ◽  
Intracellular Proteins ◽  
Enzyme Superoxide Dismutase

Studies were carried out in order to determine the presence of damaged intracellular proteins in sperm from water buffalo as a result of toxic action of free radicals and their attitude towards the vitality of the gametes. Experiments were performed with thawed semen samples as the trial has been added antioxidant enzyme superoxide dismutase. It was found that added to the test sample exogenous antioxidant makes inoffensive much of the available free radicals in sperm, respectively reduces the amount of damaged intracellular proteins and helps protect the vitality of sperm.

scholarly journals The change of antioxidant enzyme(superoxide dismutase, catalase, glutathione peroxidase) in the endotoxin infused rat lung.

1970 ◽  
Vol 40 (2) ◽  
pp. 104-111
Author(s):  
Jeong Sup Song ◽  
Chi Hong Kim ◽  
Soon Seog Kwon ◽  
Young Kyoon Kim ◽  
Kwan Hyoung Kim ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Enzyme ◽  
Rat Lung ◽  
Enzyme Superoxide Dismutase

scholarly journals The Role of Antioxidant to Prevent Free Radicals in The Body

Sains Medika ◽  
2017 ◽  
Vol 8 (1) ◽  
pp. 39 ◽  
Author(s):  
Siti Thomas Zulaikhah
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Free Radicals ◽  
Negative Impact ◽  
Reduced Glutathione ◽  
The Body ◽  
Enzyme Superoxide Dismutase ◽  
Vegetables And Fruits ◽  
Β Carotene

AbstractAntioxidants are compounds that can counteract or reduce the negative impact oxidants in the body. Antioxidants work by donating an electron to compounds that are oxidant so that the oxidant compound activity can be inhibited. Antioxidants are classified into two, namely antioxidant enzymes and non-enzyme. Antioxidant enzymes as enzyme superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), a non-enzyme antioxidant found in many vegetables and fruits, which include reduced glutathione (GSH), vitamin C, E, β- carotene, flavonoids, isoflavones, flavones, antosionin, catechins, and isokatekin, and lipoic acid. Low antioxidant enzymes can be used as a marker of high levels of free radicals in the body. Following review aims to provide an overview of the role of antioxidants in preventing the formation of free radicals in the body.

scholarly journals Superoxide anions and hyperoxia inactivate endothelium-derived relaxing factor

1986 ◽  
Vol 250 (5) ◽  
pp. H822-H827 ◽  
Author(s):  
G. M. Rubanyi ◽  
P. M. Vanhoutte
Keyword(s):  
Endothelial Cells ◽  
Superoxide Dismutase ◽  
Biological Activity ◽  
Free Radicals ◽  
Protective Effect ◽  
Femoral Artery ◽  
Superoxide Anions ◽  
Relaxing Factor ◽  
Oxygen Derived Free Radicals ◽  
Endothelium Derived Relaxing Factor

Experiments were designed to determine the effects of oxygen-derived free radicals on the production and biological activity of endothelium-derived relaxing factor or factors released by acetylcholine. Rings of canine coronary arteries without endothelium (bioassay rings) were superfused with solution passing through a canine femoral artery with endothelium. Superoxide dismutase caused maximal relaxation of the bioassay ring when infused upstream, but not downstream, of the femoral artery; this effect of superoxide dismutase was inhibited by catalase. Infusion of acetylcholine relaxed the bioassay rings because it released a labile relaxing factor (or factors) from the endothelium. When infused below the femoral artery, superoxide dismutase and, to a lesser extent, catalase augmented the relaxations to acetylcholine. Superoxide dismutase, but not catalase, doubled the half-life of the endothelium-derived relaxing factor(s). This protective effect of the enzyme was augmented fivefold by lowering the oxygen content of the perfusate from 95 to 10%. These data demonstrate that: superoxide anions inactivate the relaxing factor(s) released by acetylcholine from endothelial cells and hyperoxia favors the inactivation of endothelium-derived relaxing factor(s).

scholarly journals Regulation of superoxide dismutase activity in soybean plants by inoculating seeds with rhizobia containing nanoparticles of metal carboxylates under conditions of different water supply

2021 ◽  
Vol 29 (1) ◽  
pp. 33-38
Author(s):  
V. V. Morgun ◽  
S. Y. Kots ◽  
T. P. Mamenko ◽  
L. I. Rybachenko ◽  
P. P. Pukhtaievych
Keyword(s):  
Superoxide Dismutase ◽  
Moisture Content ◽  
Antioxidant Enzyme ◽  
Culture Medium ◽  
Root Nodules ◽  
Superoxide Dismutase Activity ◽  
Enzyme Superoxide Dismutase ◽  
Soybean Plants ◽  
Tn5 Mutant ◽  
Total Moisture Content

Soybean is one of the most profitable advanced crops in agricultural production in Ukraine and the world as a whole. Therefore, studies of means of regulation and increase in the adaptive capacity of soybeans in symbiosis with nodule bacteria under the action of unfavourable environmental factors are relevant and should be aimed at the use of complex bacterial compositions involving modern nanotechnological approaches. Nanocarboxylates of ferrum, molybdenum and germanium metals were used as components of rhizobia inoculation suspension for soybean seed treatment to study the effectiveness of their complex effect on the regulation of the activity of the key antioxidant enzyme superoxide dismutase in plants under drought. Various symbiotic systems were used, which included soybean plants and inoculation suspensions based on the active, virulent Tn5-mutant Bradyrhizobium japonicum B1-20 by adding nanoparticles of ferrum, germanium and molybdenum carboxylates to the culture medium in a ratio of 1: 1000. Citric acid was the chelator. A model drought lasting 14 days was created during the period of active fixation of atmospheric molecular nitrogen by root nodules of soybeans in the budding and flowering stages, by means of controlled watering of plants to 30% of the total moisture content. In the stage of bean formation, watering of plants was resumed to the optimal level – 60% of the total moisture content. The control was soybean plants, the seeds of which were inoculated with a suspension of rhizobia without the addition of chelated metals. The following research methods were used in the work – microbiological, physiological and biochemical. According to the results, it was found that when nanoparticles of carboxylates of ferrum, molybdenum and germanium were added to the inoculation suspension of rhizobia, there was an increase in superoxide dismutase activity in root nodules and a decrease in soybean leaves under optimal water supply conditions of plants. This indicates the initial changes in the activity of the antioxidant enzyme in these symbiotic systems, induced by the influence of chelated metals in combination with the rhizobia of the active Tn5-mutant B. japonicum B1-20. Prolonged drought induced an increase in the overall level of superoxide dismutase activity in soybean nodules and leaves, compared to plants grown under optimal watering conditions. The symbiotic system formed by soybeans and B. japonicum with molybdenum carboxylate nanoparticles was the most sensitive to long-term drought exposure, compared to two other soybean-rhizobial symbioses using ferrum and germanium nanocarboxylates. This was manifested in the unstable reaction of the enzyme to the action of drought – suppression or intensification of the level of its activity in the root nodules and leaves of soybeans inoculated with rhizobia containing molybdenum carboxylate nanoparticles. In symbiotic systems with the participation of germanium and ferrum nanocarboxylates, slight changes were revealed in superoxide dismutase activity in root nodules and leaves of plants during drought and restoration of enzyme activity to the level of plants with optimal watering after water stress. It is concluded that the addition to the culture medium of rhizobia Tn5-mutant B1-20 of nanocarboxylates of germanium or ferrum is an effective means of regulating the activity of the antioxidant enzyme superoxide dismutase in soybean root nodules and leaves, which can contribute to an increase in the protective properties and adaptation of plants to the action of dehydration.

A Fungal Cu/Zn-Containing Superoxide Dismutase Enhances the Therapeutic Efficacy of a Plant Polyphenol Extract in Experimental Influenza Virus Infection

2010 ◽  
Vol 65 (5-6) ◽  
pp. 419-428 ◽  
Author(s):  
Julia Serkedjieva ◽  
Tsvetanka Stefanova ◽  
Ekaterina Krumova
Keyword(s):  
Superoxide Dismutase ◽  
Influenza Virus ◽  
Virus Infection ◽  
Protective Effect ◽  
Influenza A ◽  
Influenza Virus Infection ◽  
Protective Role ◽  
Combined Application ◽  
Combined Use ◽  
Experimental Influenza

The combined protective effect of a polyphenol-rich extract, isolated from Geranium sanguineum L. (PC), and a novel naturally glycosylated Cu/Zn-containing superoxide dismutase, produced from the fungal strain Humicula lutea 103 (HL-SOD), in the experimental influenza A virus infection (EIVI) in mice, induced with the virus A/Aichi/2/68 (H3N2), was investigated. The combined application of HL-SOD and PC in doses, which by themselves do not defend significantly mice in EIVI, resulted in a synergistically increased protection, determined on the basis of protective indices and amelioration of lung injury. Lung weights and consolidation as well as infectious lung virus titers were all decreased significantly parallel to the reduction of the mortality rates; lung indices were raised. The excessive production of reactive oxygen species (ROS) by alveolar macrophages (aMØ) as well as the elevated levels of the lung antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), induced by EIVI, were brought to normal. For comparative reasons the combined protective effect of PC and vitamin C was investigated. The obtained results support the combined use of antioxidants for the treatment of influenza virus infection and in general indicate the beneficial protective role of combinations of viral inhibitors of natural origin with diverse modes of action.

scholarly journals Two New Compounds From the Heartwood of Dalbergia melanoxylon and Their Protective Effect on Hypoxia/Reoxygenation Injury in H9c2

2021 ◽  
Vol 16 (1) ◽  
pp. 1934578X2098777
Author(s):  
Yang Liu ◽  
Ni Zhang ◽  
Jun-wei He ◽  
Lan-ying Chen ◽  
Li Yang ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Lactate Dehydrogenase ◽  
Protective Effect ◽  
Superoxide Dismutase Activity ◽  
Spectral Evidence ◽  
Reoxygenation Injury ◽  
Dalbergia Melanoxylon ◽  
New Compounds ◽  
Hypoxia Reoxygenation

A new neoflavonoid, named as (7 R)-(-)-3′,5-dihydroxy-4′,2,4-trimethoxy-dalbergiquinol (1) and a new phenanthrenedione, named as 3′,7-dihydroxy-3,6- dimethoxy-9-phenyl-1,4-phenanthrenedione (2), together with 4 known compounds, 5- O-methyldalbergiphenol (3), 3′,7-dihydroxy-4′,3,6-trimethoxy-9-phenyl-1,4-phenanthrenedione (4), (+)-obtusafuran (5), and melanoxin (6) were isolated from the heartwood of Dalbergia melanoxylon. Their structures were elucidated on the basis of chemical and spectral evidence, as well as by comparison with literature data. Moreover, compound 1 showed a protective effect on hypoxia/reoxygenation injury in H9c2 at 10.0 μM by decreasing lactate dehydrogenase and malondialdehyde activity and enhancing superoxide dismutase activity.

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