scholarly journals Histological evaluation of bone response to bioactive ceramics as graft material in rats

2005 ◽  
Vol 55 (5-6) ◽  
pp. 461-470 ◽  
Author(s):  
Krsljak Elena ◽  
Danilovic Vesna ◽  
Teodorovic Nevenka
Keyword(s):  
Histological Evaluation ◽  
Graft Material ◽  
Bioactive Ceramics ◽  
Bone Response

Bone Response to New Modified Titanium Surface Implants in Nonhuman Primates (Papio ursinus) and Humans: Histological Evaluation

2008 ◽  
Vol 34 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Carlo Mangano ◽  
Vittoria Perrotti ◽  
Giovanna Iezzi ◽  
Antonio Scarano ◽  
Francesco Mangano ◽  
...  
Keyword(s):  
Nonhuman Primates ◽  
Titanium Surface ◽  
Histological Evaluation ◽  
Papio Ursinus ◽  
Bone Response

scholarly journals Histological evaluation of bone response to pediatric endodontic pastes: an experimental study in guinea pig

2012 ◽  
Vol 23 (6) ◽  
pp. 635-644 ◽  
Author(s):  
Andréa Mara Lacativa ◽  
Adriano M Loyola ◽  
Cassio José Alves Sousa
Keyword(s):  
Tube Wall ◽  
General Pattern ◽  
Pediatric Dentistry ◽  
Histological Evaluation ◽  
Necrotic Tissue ◽  
Pulp Therapy ◽  
Lower Jaw ◽  
Bone Response ◽  
Implant Technique ◽  
Observation Period

This study aimed to evaluate by the intra-osseous implant technique the most commonly used materials for pulp therapy in pediatric dentistry: calcium hydroxide (CH), Guedes Pinto paste and CTZ paste, according to FDI (1980) and ANSI/ADA (1982) recommendations. Thirty guinea pigs, 10 for each material, divided into experimental periods of 4 and 12 weeks received one implant on each side of the lower jaw symphysis. The external lateral tube wall served as control for the technique. At the end of the observation periods, the animals were euthanized and specimens were prepared for routine histological examination. It was observed that CH and CTZ paste induced severe inflammation, a large amount of necrotic tissue, lymphocytes, foreign body cells and bone resorption, while Guedes Pinto Paste induced little or no inflammation in the 4-week observation period. After 12 weeks, the reactions to CH and Guedes Pinto paste were also absent/mild, presenting a general pattern of replacement by recently formed bone tissue while a moderate to severe inflammatory response was observed with CTZ paste. Guedes Pinto paste presented acceptable biocompatibility levels in both analyzed periods; CH only showed acceptable biocompatibility in the 12-week period while CTZ paste showed no biocompatibility in both periods. Among the tested materials, only Guedes Pinto paste presented an acceptable biocompatibility.

Histological evaluation of the bone response to calcium phosphate cement implanted in cortical bone

Biomaterials ◽  
2003 ◽  
Vol 24 (6) ◽  
pp. 989-1000 ◽  
Author(s):  
E.M Ooms ◽  
J.G.C Wolke ◽  
M.T van de Heuvel ◽  
B Jeschke ◽  
J.A Jansen
Keyword(s):  
Calcium Phosphate ◽  
Cortical Bone ◽  
Calcium Phosphate Cement ◽  
Histological Evaluation ◽  
Bone Response

Fabrication, characterization and application of polypropylene macroporous mesh for repairing pelvic floor defects

2016 ◽  
Vol 87 (7) ◽  
pp. 878-888
Author(s):  
Yao Lu ◽  
Sheng-nan Dong ◽  
Yan-chun Chen ◽  
Pei-hua Zhang ◽  
Xiao-wen Tong
Keyword(s):  
Pelvic Floor ◽  
Pore Size ◽  
Structural Parameters ◽  
Pelvic Floor Dysfunction ◽  
Postoperative Recovery ◽  
Tissue Growth ◽  
Histological Evaluation ◽  
Good Mechanical Property ◽  
Graft Material ◽  
Good Biocompatibility

The application of mesh to reconstruct pelvic floors is considered a state-of-the-art method for the treatment of pelvic floor dysfunction. Polypropylene (PP) is the most frequently used synthetic graft material in gynecology departments due to its good mechanical property and non-degradability. Pelvic repairing meshes are required to have good biocompatibility, stability and low shrinkage with sufficient softness. Meshes should also adhere to surrounding tissues to support pelvic organs after implantation. This work designed two kinds of lightweight PP macroporous meshes with different structures. The meshes were designed based on analyzing properties of Gynemesh® PS, which is a common pelvic repairing mesh in clinical use. The requirements of mesh design were as follows: mesh weight <45 g/m2, pore size >75 µm, porosity >60%. Two kinds of meshes were fabricated according to design requirements and named the S1 mesh and S2 mesh. Several structural parameters, including thickness, weight, pore size, porosity and mechanical properties, including bursting strength, tensile strength, suture pulling off force and flexibility, were tested and analyzed. S1 mesh and S2 mesh were then implanted in the onlay position of rabbits for 90 days. The biocompatibility was evaluated through operation situation, postoperative recovery, mesh adhesion, shrinkage, histological evaluation of mesh and surrounding tissues. The results revealed that the two kinds of meshes were both beneficial to promote tissue growth; most animals recovered well. However, S1 mesh had greater shrinkage, while there existed one case of infection in the S2 mesh group because of mesh folding.

Evaluation of TCP Loaded Clinoptilolite Use as Graft Material on Rabbit Tibia

2011 ◽  
Vol 493-494 ◽  
pp. 175-180 ◽  
Author(s):  
A.N. Uyumaz ◽  
L.S. Ozyegin ◽  
N. Buyukakyuz ◽  
B. Yesilbek ◽  
F.N. Oktar
Keyword(s):  
Bone Formation ◽  
Inflammatory Cell ◽  
Cell Formation ◽  
Histological Evaluation ◽  
Control Group ◽  
Graft Material ◽  
New Bone Formation ◽  
Rabbit Tibia ◽  
Dental Applications ◽  
Ketamine Hcl

Clinoptilolite mineral which is a member of zeolite family has been recently used in medical and dental applications. Until today, it has never been used as a graft material. In the present study tricalcium phosphate (TCP) imbued 90-95% pure clinoptilolite was used as graft in rabbit tibia. General anesthesia was accomplished using intra muscular (IM) injections of Xylazin and Ketamine HCL. TCP imbued clinoptilolite was placed in the defects created in the tibias of the subjects. Control group defects were left empty. One group was sacrificed on day 28 and the other group on day 56 to evaluate osteogenesis, residual graft material, inflammation and fibrosis. Histological evaluation revealed new bone formation at 28 days to be 14/7 at control group where as its 11/7 for the experimental graft group. At 56 days the values are as 18/7 for control and 14/7 for the experimental graft groups (n = 7). Our study group is the first to perform intrabony application of clinoptilolite. No evidence of abnormal inflammatory cell formation or fibrosis was witnessed in groups. When TCP, which can be used as a standalone graft material, was mixed with zeolite it was not as effective as expected. We believe that, TCP particles are absorbed and captivated in the initial phase and due to absorption strength of zeolite cannot be released back to the environment. We assume its effect can increase with longer time periods. Bone formation without infection is observed around graft particles. Based on the present study, since clinoptilolite does not provoke an inflammatory process, its use in unison with TCP can provide a supporting structure in defects.

scholarly journals Histomorphometric Evaluation of Peri-Implant Bone Response to Intravenous Administration of Zoledronate (Zometa®) in an Osteoporotic Rat Model

Materials ◽  
2020 ◽  
Vol 13 (22) ◽  
pp. 5248
Author(s):  
Amani M. Basudan ◽  
Marwa Y. Shaheen ◽  
Abdurahman A. Niazy ◽  
Jeroen J. J. P. van den Beucken ◽  
John A. Jansen ◽  
...  
Keyword(s):  
Femoral Condyle ◽  
Titanium Implants ◽  
Histological Evaluation ◽  
Bone Area ◽  
Treatment Regimens ◽  
Implant Placement ◽  
Bone Response ◽  
Female Wistar Rats ◽  
The Right ◽  
Post Implantation

We evaluated the response to peri-implant bone placed in the femoral condyle of osteoporotic rats, following intravenous zoledronate (ZOL) treatment in three settings: pre-implantation (ZOL-Pre), post-implantation (ZOL-Post), and pre- + post-implantation (ZOL-Pre+Post). Twenty-four female Wistar rats were ovariectomized (OVX). After 12 weeks, the rats received titanium implants in the right femoral condyle. ZOL (0.04 mg/kg, weekly) was administered to six rats 4 weeks pre-implantation and was stopped at implant placement. To another six rats, ZOL was given post-implantation and continued for 6 weeks. Additional six rats received ZOL treatment pre- and post-implantation. Control animals received weekly saline intravenous injections. At 6 weeks post-implantation, samples were retrieved for histological evaluation of the percentage of bone area (%BA) and of the percentage of bone-to-implant contact (%BIC). BA% for ZOL-Pre (29.6% ± 9.0%) and ZOL-Post (27.9% ± 5.6%) rats were significantly increased compared to that of the controls (17.3% ± 3.9%, p < 0.05). In contrast, ZOL-Pre+Post rats (20.4% ± 5.0%) showed similar BA% compared to Saline controls (p = 0.731). BIC% revealed a significant increase for ZOL-Post (65.8% ± 16.9%) and ZOL-Pre+Post (68.3% ± 10.0%) rats compared with that of Saline controls (43.3% ± 9.6%, p < 0.05), while ZOL-Pre rats (55.6% ± 19%) showed a BIC% comparable to that of Saline controls (p = 0.408). Our results suggest that receiving intravenous ZOL treatment before or after implant placement enhances peri-implant bone responses in terms of bone area. However, the effect of different ZOL treatment regimens on BIC% was found to be inconclusive.

scholarly journals Fusion capacity after the implantation of interspinous fusion device - a biomechanical analysis and preliminary histological evaluation in pigs

2021 ◽  
Author(s):  
Li Bao ◽  
Mengmeng Chen ◽  
Xiang Ji ◽  
Qinchao Sun ◽  
Hai Tang
Keyword(s):  
Bone Tissue ◽  
Toluidine Blue ◽  
Lumbar Vertebrae ◽  
Test System ◽  
Biomechanical Analysis ◽  
Histological Evaluation ◽  
Blue Staining ◽  
Graft Material ◽  
Fusion Device ◽  
Flexion Extension

Abstract BackgroudThe interspinous fusion device (ISFD) was widely used in the degenerative lumbar diseases. Previous clinical studies have demonstrated favorable efficacy. To further explore interspinous fusion capacity after ISFD implantation, we performed biomechanical analysis and preliminary histological evaluation.MethodsTwelves experimental pigs were selected and accepted a simulated operation of ISDF implantation. The animals were grouped six groups based on different procedures: sham surgery; unilateral laminotomy (UL); bilateral laminotomy (BL); ISFD + intact; ISFD + UL; ISFD + BL. The graft-bed site was filled with purified bone graft material without any autograft bone. After six months feeding, all experimental animals were sacrificed and the corresponding lumbar vertebrae was obtained. The samples were fixed on the spinal test system and the motion range of flexion-extension, lateral bending and rotation were tested through a multiaxial robotic system. The samples were prepared to cut using a hard tissue-slicing machine. Then, tissue sections stained with hematoxylin-eosin (HE) and toluidine blue were observed to evaluate the new-bone formation.ResultsLaminotomy increased lumbar mobility in comparison with intact model, especially for BL. The implantation of ISDF can reduce the range of motion in all motion directions. Well-defined bone tissue can be observed in the histological images after 6 months. In the histological part of toluidine blue staining, the area of newly formed bone accounted for 8.721%. The hyperchromatic osteoblasts cells and density bone tissue can be observed in HE staining slides.ConclusionsThe interspinous osteogenesis in histology confirmed the possibility of interspinous fusion. Moreover, the implantation of ISDF provide the necessary stabilization for promoting fusion.

Histological evaluation of cochlear hair cell damage from noise-induced hearing loss in chinchillas

1990 ◽  
Vol 48 (3) ◽  
pp. 332-333
Author(s):  
R.V. Harrison ◽  
R.J. Mount ◽  
P. White ◽  
N. Fukushima
Keyword(s):  
Hair Cell ◽  
Evoked Potential ◽  
Cell Damage ◽  
Acoustic Trauma ◽  
Histological Evaluation ◽  
Cell Integrity ◽  
Functional Changes ◽  
Cochlear Hair Cell ◽  
Left And Right ◽  
Contralateral Ear

In studies which attempt to define the influence of various factors on recovery of hair cell integrity after acoustic trauma, an experimental and a control ear which initially have equal degrees of damage are required. With in a group of animals receiving an identical level of acoustic trauma there is more symmetry between the ears of each individual, in respect to function, than between animals. Figure 1 illustrates this, left and right cochlear evoked potential (CAP) audiograms are shown for two chinchillas receiving identical trauma. For this reason the contralateral ear is used as control.To compliment such functional evaluations we have devised a scoring system, based on the condition of hair cell stereocilia as revealed by scanning electron microscopy, which permits total stereociliar damage to be expressed numerically. This quantification permits correlation of the degree of structural pathology with functional changes. In this paper wereport experiments to verify the symmetry of stereociliar integrity between two ears, both for normal (non-exposed) animals and chinchillas in which each ear has received identical noise trauma.

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