scholarly journals Antioxidant activity of white grape seed extracts on DPPH radicals

2009 ◽  
pp. 53-61 ◽  
Author(s):  
Anamarija Mandic ◽  
Sonja Djilas ◽  
Jasna Canadanovic-Brunet ◽  
Gordana Cetkovic ◽  
Jelena Vulic
Keyword(s):  
Antioxidant Activity ◽  
Grape Seed ◽  
Dpph Radical ◽  
Grape Seeds ◽  
Food Ingredient ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Red Grape ◽  
Grape Varieties ◽  
Grape Seed Extracts

Composition and antioxidant activity of grape seed extract (GSE) obtained from red grape varietes are very well documented, in contrast to the white varietes. This paper presents the results of polyphenols content of ethyl acetate extract of grape seeds, obtained from two white grape varieties, Italian Riesling and Zupljanka, and their antioxidant activity on the stable DPPH radical. The influence of the addition of GSE to raspberry juice on the DPPH radical was also examined. Content of total polyphenols in GSEs ranged between 81.6 and 82.8% (w/w), and the contetn of flavan-3-ols between 66.2 and 91.0% (w/w). HPLC results showed that the most abundant components in the extract were (+)-catechin and (-)-epicatechin for both grape varieties. All tested GSEs exhibited good antioxidant activity. IC50 values for the GSEs of Italian Riesling and Zupljanka were 0.79 and 0.95 mg sample/mg DPPH radical, respectivelly. Since the GSE of Italian Riesling possesed stronger antioxidant activity, it was used for further experiments. The IC50 value for raspberry juice was 4.18 mg raspberry juice/mg DPPH. The raspberry juice with addition of 0.60 ?g/mL of GSE showed antioxidant activity of 39.2%. The same juice with the threefold concentration of vitamin C (1.81 ?g/ml) exhibited similar antioxidant activity (33.9%). Antioxidant activity of the same amount of juice without added antioxidants was lower (15.7%). The results showed that the GSE of white varietes could be considered as a good functional food ingredient.

scholarly journals Flavanol Glycoside Content of Grape Seeds and Skins of Vitis vinifera Varieties Grown in Castilla-La Mancha, Spain

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 4001 ◽  
Author(s):  
José Pérez-Navarro ◽  
Guillaume Cazals ◽  
Christine Enjalbal ◽  
Pedro Miguel Izquierdo-Cañas ◽  
Sergio Gómez-Alonso ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
High Performance ◽  
Grape Seed ◽  
Grape Seeds ◽  
Grape Berries ◽  
La Mancha ◽  
Liquid Chromatography Tandem Mass ◽  
Grape Varieties ◽  
Grape Seed Extracts ◽  
Seed Extracts

Glycosylated flavanols (monoglycosides and diglycosides) in skin and seed extracts of Vitis vinifera grapes grown in Castilla-La Mancha (Spain) were investigated using ultra-high-performance liquid chromatography—tandem mass spectrometry (UHPLC-ESI-QQQ-MS/MS). Six grape varieties (Airén, Tempranillo, the recently identified Albillo Dorado, Montonera del Casar, Moribel, and Tinto Fragoso) were studied over two consecutive years (2016 and 2017). A total of twenty monomeric flavanol monoglycosides, four diglycosylated monomers, and three dimeric flavanol monoglycosides were detected in all grape samples. The diversity observed in the composition of glycosylated flavanol in the grape berries suggests a strong influence of variety and grape tissue (skin or seed). Monomeric flavanol glycosides were more abundant in grape seed extracts, in contrast with monoglycosylated dimeric forms. In addition, the glycosylated flavanol content was related to berry color in grape skins, with higher concentrations measured in black grape varieties.

scholarly journals ANTIOXIDANT ACTIVITIES OF HYDROLYSATES ORIGINATED FROM SOYBEAN AND SOY MILK RESIDUE

2018 ◽  
Vol 55 (5A) ◽  
pp. 134
Author(s):  
Ngo Minh Ngoc
Keyword(s):  
Antioxidant Activity ◽  
Protein Content ◽  
Manufacturing Industry ◽  
Antioxidant Activities ◽  
Proteolytic Enzymes ◽  
Radical Scavenging ◽  
Soy Milk ◽  
Dpph Radical ◽  
Ic50 Value ◽  
Ic50 Values

Soybean (Glycine max) and soy milk residue (okara) are protein-rich materials. Soybean possesses the highest protein content among different types of beans (protein content of soybean varies from 40–42 %). Soy milk residue, a by-product of the soy milk manufacturing industry, contains approximately 27 % protein (by dry weight). A number of recent studies have investigated the improvement of functional properties of protein contained in soybean and okara by fermentation or by the use of proteolytic enzymes. The aim of this study was to evaluate the antioxidant activities of soybean and okara hydrolysates obtained by the fermentation with Aspergillus oryzae or by using proteolytic enzymes (neutrase and flavourzyme). DPPH radical scavenging assay was used to determine the antioxidant activities of hydrolysates. The concentration of peptides required to scavenge DPPH radical by 50 % (IC50 value) was used to evaluate the antioxidant activity of peptides produced obtained from hydrolysates. The results showed that when fermented with A. oryzae, the okara hydrolysate had higher antioxidant activity than the soybean hydrolysate, with IC50 values of 0.447 mg/ml and 3.95 mg/ml, respectively. The hydrolyzed okara obtained from hydrolysis using Neutrase had higher antioxidant activity than the one obtained from hydrolysis using Flavourzyme, with IC50 values of0.200 mg/ml and 0.407 mg/ml, respectively. Different peptide fractions obtained from the hydrolysates using cut-off membrane (10 kDa, 3 kDa and 1 kDa) possessed different antioxidant activities. The < 1 kDa peptide fraction exhibited the highest antioxidant activity with an IC50 value of 0.158 mg/ml.

scholarly journals Characterization of the chemical and nutritive quality of coldpressed grape seed oils produced in the Republic of Serbia from different red and white grape varieties

2021 ◽  
Vol 72 (2) ◽  
pp. e411
Author(s):  
V.B. Vujasinović ◽  
M.M. Bjelica ◽  
S.C. Čorbo ◽  
S.B. Dimić ◽  
B.B. Rabrenović
Keyword(s):  
Seed Oil ◽  
Grape Seed ◽  
Total Phenolic ◽  
Seed Oils ◽  
Grape Seeds ◽  
Nutritive Quality ◽  
Grape Seed Oil ◽  
The Republic ◽  
Red Grape ◽  
Grape Varieties

Six cold-pressed oil samples obtained from the seeds of different grape varieties grown in a Fruška Gora vineyard in the Republic of Serbia were examined for chemical and nutritive quality, as well as antiradical capacity. All the tested samples were of good quality, but the results showed noticeable differences in seed oil properties for red and white grape varieties. The highest content in total tocols, 575.23 ± 4.46 mg/kg, was found in the red grape seed oil of the Merlot variety, but the vitamin E activity of white grape seed oils was significantly higher. Regarding single phenols, the most prevalent fraction in all the oil samples was ursolic acid, up to 336.3 ± 4.8 μg/g in the grape seed oil of the Hamburg variety. The highest content in the total phenolic compounds, 54.92 ± 0.93 μg GAE/g, was detected in the oil of red grape seeds. Although significant differences existed between samples, EC50 values were the lowest for the red grape seed oils, varying from 29.84 ± 1.07 to 65.34 ± 0.32 mg oil/mg DPPH, indicating that these oils had the highest antiradical capacity.

Effect of heating conditions of grape seeds on the antioxidant activity of grape seed extracts

2006 ◽  
Vol 97 (3) ◽  
pp. 472-479 ◽  
Author(s):  
S KIM ◽  
S JEONG ◽  
W PARK ◽  
K NAM ◽  
D AHN ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Grape Seed ◽  
Grape Seeds ◽  
Grape Seed Extracts ◽  
Seed Extracts

scholarly journals Antioxidant, Antimicrobial and Cytotoxic Activities of Samanea saman (Jacq.) Merr.

1970 ◽  
Vol 3 (1) ◽  
pp. 11-17 ◽  
Author(s):  
Afia Ferdous ◽  
Mohammad Zafar Imam ◽  
Tajnin Ahmed
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Brine Shrimp ◽  
Dpph Radical ◽  
Cytotoxic Potential ◽  
Ic50 Value ◽  
Total Antioxidant ◽  
Samanea Saman ◽  
Soluble Fractions

In the present investigation the n-hexane, carbon tetrachloride and choloroform soluble fractions of crude methanolic extract of Samanea saman bark were tested for antioxidant, antimicrobial and cytotoxic potential. Antioxidant activity of the extracts was evaluated by DPPH radical scavenging assay and total antioxidant activity test. Antimicrobial activity was tested using disc diffusion method against thirteen bacteria and three fungi and cytotoxicity was tested by brine shrimp lethality bioassay. Chloroform and hexane soluble fraction showed IC50 value of 12μg/ml and 14μg/ml respectively in scavenging DPPH radical while the reference Butylated hydroxytoluene showed an IC50 value of 10μg/ml. The carbon tetrachloride fraction showed the highest total antioxidant capacity. The carbon tetrachloride fraction was also found to possess mild to moderate microbial growth inhibitory capacity. In the brine shrimp lethality bioassay, the n-hexane, carbon tetrachloride, chloroform soluble fractions showed LC50 value of 14.94μg/ml, 0.831μg/ml and 3.288μg/ml respectively. The results suggest good antioxidant and cytotoxic potential of chloroform and hexane soluble fractions and antimicrobial activity of carbon tetrachloride fraction of Samanea saman bark extract. Key Words: Samanea saman; Leguminoseae; Cytotoxicity; Antimicrobial; Antioxidant; Total antioxidant capacity. DOI: 10.3329/sjps.v3i1.6792S. J. Pharm. Sci. 3(1): 11-17

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals Characterization of fatty acid, antioxidant, and polyphenol content of grape seed oil from different Vitis vinifera L. varieties

OCL ◽  
2021 ◽  
Vol 28 ◽  
pp. 30
Author(s):  
Viktória Kapcsándi ◽  
Erika Hanczné Lakatos ◽  
Beatrix Sik ◽  
László Ádám Linka ◽  
Rita Székelyhidi
Keyword(s):  
Seed Oil ◽  
Grape Seed ◽  
Seed Oils ◽  
Grape Seeds ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Total Polyphenol Content ◽  
Grape Seed Oil ◽  
Total Antioxidant ◽  
Grape Varieties

In this study, we examined the yield and oil quality of eight different grape varieties. For the experiments, the grape seeds were obtained from the Benedictine Pannonhalma Archabbey in the northwestern region of Hungary. The aim of the studies was to determine the oil yield obtained by extraction and to examine the differences between the fatty acid composition, antioxidant capacity, and total polyphenol content of the oils of different grape varieties. Based on the results, the oil content of the grape seeds varied between 99.91 g/kg and 126.74 g/kg. The grape seed oils analysed contained significant amounts of stearic acid (3.42–9.93%), palmitic acid (7.81–10.66%), oleic acid (14.29–19.92%), and linoleic acid (66.85–72.47%). Besides, the grape seed oils tested contained several other fatty acids in small amounts. There were significant differences in the total antioxidant and total polyphenol content of the oils. Total polyphenol content ranged from 0.24 to 1.13 mg GAE/g, while the total antioxidant content changed between 0.12 and 0.78 μg TEAC/g. The results show that the studied varieties are suitable for the production of table grape seed oil based on their oil yield, and the oils have favourable, health-protecting properties in terms of their quality.

scholarly journals ANTIOXIDANT ACTIVITY OF METHYL GALLATE ISOLATED FROM THE LEAVES OF Toona sureni

2010 ◽  
Vol 9 (3) ◽  
pp. 457-460 ◽  
Author(s):  
M. Taufik Ekaprasada ◽  
Hazli Nurdin ◽  
Sanusi Ibrahim ◽  
Dachriyanus Dachriyanus
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Methyl Gallate ◽  
Antioxidant Compound ◽  
Ic50 Value ◽  
Isolated Compound ◽  
Dpph Radical Scavenging

An antioxidant compound has been isolated from the leaves of Toona sureni (Blume) Merr. The structure was determined to be methyl 3,4,5-trihydroxybenzoate (methyl gallate), based on UV-vis, FTIR, NMR and MS spectra. The isolated compound exhibited potent antioxidant activity in the α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging test, with IC50 value 1.02 μg/mL.   Keywords: antioxidant activity, methyl gallate, Toona sureni

scholarly journals Optimization of Catechin and Proanthocyanidin Recovery from Grape Seeds Using Microwave-Assisted Extraction

Biomolecules ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 243
Author(s):  
Jing Chen ◽  
W. P. D. Wass Thilakarathna ◽  
Tessema Astatkie ◽  
H. P. Vasantha Rupasinghe
Keyword(s):  
Total Yield ◽  
Dry Weight ◽  
Grape Seed ◽  
Microwave Assisted Extraction ◽  
Grape Seeds ◽  
Epicatechin Gallate ◽  
Food Ingredient ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Potential Applicability

Grape seed extract (GSE) is a rich source of condensed flavonoid tannins, also called proanthocyanidins (PACs). The high molecular weight of polymeric PAC limits their biological activity due to poor bioavailability. The present study was undertaken to explore the potential applicability of microwave-assisted extraction (MAE) to convert GSE-PAC into monomeric catechins. A central composite design (CCD) was used to optimize the processing conditions for the MAE. The maximum total yield of monomeric catechins (catechin, epicatechin, and epicatechin gallate) and PAC were 8.2 mg/g dry weight (DW) and 56.4 mg catechin equivalence (CE)/g DW, respectively. The optimized MAE condition was 94% ethanol, 170 °C temperature, and a duration of 55 min. Compared to the results for PACs extracted via conventional extraction (Con) (94% ethanol; shaking at 25 °C for 55 min), MAE yielded 3.9-fold more monomeric catechins and 5.5-fold more PACs. The MAE showed higher antioxidant capacity and α-glucosidase inhibitory activity than that of the conventional extract, suggesting the potential use of the MAE products of grape seeds as a functional food ingredient and nutraceutical.

Anthocyanins profile, total phenolics and antioxidant activity of two Romanian red grape varieties: Feteascǎ neagrǎ and Bǎbeascǎ neagrǎ (Vitis vinifera)

2015 ◽  
Vol 69 (12) ◽  
Author(s):  
Oana Emilia Constantin ◽  
Mihaela Skrt ◽  
Natasa Poklar Ulrih ◽  
Gabriela Râpeanu
Keyword(s):  
Antioxidant Activity ◽  
Vitis Vinifera ◽  
High Performance ◽  
Total Phenolics ◽  
High Performance Liquid Chromatographic ◽  
Phenolic Composition ◽  
Total Anthocyanins ◽  
Red Grape ◽  
Grape Varieties ◽  
Liquid Chromatographic

AbstractThe phenolic composition of Fetească neagră and Băbească neagră grapes from Dealul Bujorului vineyard (south-east Romania) was studied using the spectrophotometric and high-performance liquid chromatographic (HPLC) methods. The results revealed significant differences between these cultivars. Total anthocyanins ranged from 0.22-5.98 mg g

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