scholarly journals Optimization of Catechin and Proanthocyanidin Recovery from Grape Seeds Using Microwave-Assisted Extraction

Biomolecules ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 243
Author(s):  
Jing Chen ◽  
W. P. D. Wass Thilakarathna ◽  
Tessema Astatkie ◽  
H. P. Vasantha Rupasinghe
Keyword(s):  
Total Yield ◽  
Dry Weight ◽  
Grape Seed ◽  
Microwave Assisted Extraction ◽  
Grape Seeds ◽  
Epicatechin Gallate ◽  
Food Ingredient ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Potential Applicability

Grape seed extract (GSE) is a rich source of condensed flavonoid tannins, also called proanthocyanidins (PACs). The high molecular weight of polymeric PAC limits their biological activity due to poor bioavailability. The present study was undertaken to explore the potential applicability of microwave-assisted extraction (MAE) to convert GSE-PAC into monomeric catechins. A central composite design (CCD) was used to optimize the processing conditions for the MAE. The maximum total yield of monomeric catechins (catechin, epicatechin, and epicatechin gallate) and PAC were 8.2 mg/g dry weight (DW) and 56.4 mg catechin equivalence (CE)/g DW, respectively. The optimized MAE condition was 94% ethanol, 170 °C temperature, and a duration of 55 min. Compared to the results for PACs extracted via conventional extraction (Con) (94% ethanol; shaking at 25 °C for 55 min), MAE yielded 3.9-fold more monomeric catechins and 5.5-fold more PACs. The MAE showed higher antioxidant capacity and α-glucosidase inhibitory activity than that of the conventional extract, suggesting the potential use of the MAE products of grape seeds as a functional food ingredient and nutraceutical.

scholarly journals Microwave-Assisted Extraction of Phenolic Compounds from Melastoma sanguineum Fruit: Optimization and Identification

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2498 ◽  
Author(s):  
Cai-Ning Zhao ◽  
Jiao-Jiao Zhang ◽  
Ya Li ◽  
Xiao Meng ◽  
Hua-Bin Li
Keyword(s):  
Phenolic Compounds ◽  
Irradiation Time ◽  
Soxhlet Extraction ◽  
Dry Weight ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Epicatechin Gallate ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Temperature Time

A microwave-assisted extraction (MAE) technology optimized by response surface methodology (RSM) was established to extract phenolic compounds from the fruit of Melastoma sanguineum. The effects of solvent composition, ratio of solvent to material, temperature, time and microwave power on phenol yield were evaluated in single-factor tests. The three parameters exerting main impacts on phenol yield were further optimized by RSM. Under optimal extraction conditions (31.33% ethanol, solvent/material ratio of 32.21 mL/g, 52.24 °C, 45 min and 500 W), the total phenolic content was 39.02 ± 0.73 mg gallic acid equivalent (GAE)/g dry weight (DW). This MAE method performed better in comparison with two conventional methods, those being maceration (25.79 ± 1.03 mg GAE/g DW) and Soxhlet extraction (18.40 ± 1.34 mg GAE/g DW), using lower process temperature, shorter irradiation time, and lower organic solvent consumption. In addition, five flavonoids (epicatechin gallate, epicatechin, rutin, pigallocatechin and quercetin) and two phenolic acids (protocatechuic acid and chlorogenic acid) in the extract were identified and quantified using UPLC-MS/MS.

scholarly journals Evaluation of Extracts Obtained from FruitWastes Using Different Methods

Ingeniería ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 77-92
Author(s):  
Ciliana Florez Montes ◽  
Andrés Felipe Rojas González ◽  
Sneyder Rodríguez Barona
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Total Phenolic Compounds ◽  
Grape Seeds ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Pharmaceutical Industries

Context: Currently, the increase in agroindustrial waste generation has encouraged the search for viable use alternatives. In this paper, four methods to obtain extracts from mango, soursop, and grape peels, as well as and grape seeds, are studied. Their efficiency is analyzed through extraction yields and antioxidant capacity characterization of the extracts. Method: The extraction was performed using solvent, Soxhlet, microwave-assisted, and ultrasound assisted extraction. The characterization of the extracts was made by total phenolic compounds and flavonoids quantification, as well as antioxidant capacity determination, using the DPPH, FRAP, and ORAC tests. Results: It was found that grape seed extracts obtained by different extraction methods, highlighting those obtained by microwave assisted extraction, present a high total content phenolic compounds (>321.381,41 ± 3.476,85 μg Gallic Acid/g) and flavonoids (>103.232,01 ± 4.638,19 μg Quercetin/g), in addition to high antioxidant activity, according to the results of the DPPH (<1,06 ± 0,01), FRAP (>152.280,08 ± 5.197,53 µg TROLOX/g), and ORAC (>124.566,81 ± 581,96 μg TROLOX/g) tests. Conclusions: The results presented in this study suggest that the extracts obtained from grape seeds, especially those obtained by means of microwave-assisted extraction, have a potential use in food and pharmaceutical industries, due to their high antioxidant capacity and their total phenolic compounds and flavonoids content.

scholarly journals Total Saponins Isolated from Corni Fructus via Ultrasonic Microwave-Assisted Extraction Attenuate Diabetes in Mice

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 670
Author(s):  
Shujing An ◽  
Dou Niu ◽  
Ting Wang ◽  
Binkai Han ◽  
Changfen He ◽  
...  
Keyword(s):  
Glucose Transporter ◽  
Diabetic Patients ◽  
Microwave Assisted Extraction ◽  
Diabetic Mice ◽  
Phosphatidylinositol 3 Kinase ◽  
Food Ingredient ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Pharmaceutical Industries ◽  
Corni Fructus

Saponins have been extensively used in the food and pharmaceutical industries because of their potent bioactive and pharmacological functions including hypolipidemic, anti-inflammatory, expectorant, antiulcer and androgenic properties. A lot of saponins-containing foods are recommended as nutritional supplements for diabetic patients. As a medicine and food homologous material, Corni Fructus (CF) contains various active ingredients and has the effect of treating diabetes. However, whether and how CF saponins attenuate diabetes is still largely unknown. Here, we isolated total saponins from CF (TSCF) using ultrasonic microwave-assisted extraction combined with response surface methodology. The extract was further purified by a nonpolar copolymer styrene type macroporous resin (HPD-300), with the yield of TSCF elevated to 13.96 mg/g compared to 10.87 mg/g obtained via unassisted extraction. When used to treat high-fat diet and streptozotocin-induced diabetic mice, TSCF significantly improved the glucose and lipid metabolisms of T2DM mice. Additionally, TSCF clearly ameliorated inflammation and oxidative stress as well as pancreas and liver damages in the diabetic mice. Mechanistically, TSCF potently regulated insulin receptor (INSR)-, glucose transporter 4 (GLUT4)-, phosphatidylinositol 3-kinase (PI3K)-, and protein kinase B (PKB/AKT)-associated signaling pathways. Thus, our data collectively demonstrated that TSCF could be a promising functional food ingredient for diabetes improvement.

scholarly journals Optimization of the Microwave-Assisted Extraction of Simple Phenolic Compounds from Grape Skins and Seeds

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1527
Author(s):  
Latifa Azaroual ◽  
Ali Liazid ◽  
Fouad El Mansouri ◽  
Jamal Brigui ◽  
Ana Ruíz-Rodriguez ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Extraction Time ◽  
Microwave Assisted Extraction ◽  
Grape Seeds ◽  
Intermediate Precision ◽  
Grape Skin ◽  
Extraction Solvent ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Relative Standard

A method for the extraction of phenolic compounds from grape seeds and skins using microwave-assisted extraction (MAE) was developed. Optimization of the effects of the extraction parameters in terms of the results of extraction was obtained using the response surface methodology. The parameters studied were extraction solvent (methanol, ethanol, acetone, and water), percentage of methanol in water, quantity of sample in relation to volume of extraction solvent (solid:liquid, 10–50 mg mL−1), power (100–500 W), magnetic stirring speed (0–100%), and extraction time (5–20 min). Finally, the repeatability and the intermediate precision of the method were determined. The best conditions proved to be: 65% methanol in water as an optimum extraction solvent; 0.5 g of grape skin or seed in a volume of 25 mL; a power of 500 W with the maximum stirring speed (100%); and an extraction time of 5 min. The phenolic compounds proved to be stable in the optimized extraction conditions. The resulting repeatability and the intermediate precision of the optimized method showed a relative standard deviation below 7%. The new method applied on Napoleon grape allowed for the determination of catechin (453.2 (mg kg−1)), epicatechin (306.3 mg kg−1), caftaric acid (22.37 mg caffeic acid equivalents kg−1), dihydrokaempferol-glycoside (11.13 mg kaempferol equivalents kg−1), quercetin (18.28 mg kg−1), quercetin-3-glucoside (20.09 mg quercetin equivalents kg−1), and kaempferol-3-glucoside (11.10 mg kaempferol equivalents kg−1).

Microwave-assisted Extraction of Phenolic Compounds from Grape Seed

2001 ◽  
Vol 15 (3) ◽  
pp. 197-204 ◽  
Author(s):  
Ni Hong ◽  
Varoujan A. Yaylayan ◽  
G. S. Vijaya Raghavan ◽  
J. R. Jocelyn Paré ◽  
Jacqueline M. R. Bélanger
Keyword(s):  
Phenolic Compounds ◽  
Grape Seed ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction

scholarly journals Identification and Quantification of β-Sitosterol β-d-Glucoside of an Ethanolic Extract Obtained by Microwave-Assisted Extraction from Agave angustifolia Haw

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3926
Author(s):  
Herminia López-Salazar ◽  
Brenda Hildeliza Camacho-Díaz ◽  
Sandra Victoria Ávila-Reyes ◽  
Ma Dolores Pérez-García ◽  
Manases González- Cortazar ◽  
...  
Keyword(s):  
High Performance ◽  
Ethanolic Extract ◽  
Dry Weight ◽  
Extraction Time ◽  
Ionization Mass ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Agave Angustifolia ◽  
Assisted Extraction ◽  
Ft Ir

β-sitosterol β-d-glucoside (BSSG) was extracted from “piña” of the Agave angustifolia Haw plant by microwave-assisted extraction (MAE) with a KOH solution such as a catalyst and a conventional maceration method to determine the best technique in terms of yield, extraction time, and recovery. The quantification and characterization of BSSG were done by high-performance thin layer chromatography (HPTLC), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography−electrospray ionization−mass spectrometry (HPLC-ESI-MS). With an extraction time of 5 s by MAE, a higher amount of BSSG (124.76 mg of β-sitosterol β-d-glucoside/g dry weight of the extract) than those for MAE extraction times of 10 and 15 s (106.19 and 103.97 mg/g dry weight respectively) was shown. The quantification of BSSG in the extract obtained by 48 h of conventional maceration was about 4–5 times less (26.67 mg/g dry weight of the extract) than the yields reached by the MAE treatments. MAE achieved the highest amount of BSSG, in the shortest extraction time while preserving the integrity of the compound’s structure.

Orthogonal test in optimizing microwave-assisted extraction process of magnolin from Magnoliae Flos

2010 ◽  
Vol 30 (5) ◽  
pp. 567-568
Author(s):  
Xiao-li LI ◽  
Ming-yuan ZHANG ◽  
Wei-quan ZHAO ◽  
Man Li ◽  
Hai-ying TENG ◽  
...  
Keyword(s):  
Extraction Process ◽  
Orthogonal Test ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction
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