scholarly journals Influence of trout farm effluents on selected oxidative stress biomarkers in larvae of Ecdyonurus venosus (Ephemeroptera, Heptageniidae)

2019 ◽  
Vol 71 (2) ◽  
pp. 225-233
Author(s):  
Andjelina Radojevic ◽  
Dejan Mircic ◽  
Miroslav Zivic ◽  
Vesna Peric-Mataruga ◽  
Milenka Bozanic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Fish Farm ◽  
Chemical Parameters ◽  
Ammonium Ions ◽  
Total Glutathione ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Trout Farms ◽  
Physical And Chemical ◽  
Biomarkers Of Oxidative Stress

The aim of the present study was to establish the extent to which the outlet waters of trout farms affect the ecosystems of the Crnica and Skrapez rivers in Serbia. We monitored selected biomarkers of oxidative stress: superoxide dismutase (SOD), glutathione peroxidase (GPx) and total glutathione (GSH) in larvae of the species Ecdyonurus venosus, and simultaneously analyzed the changes in the physical and chemical parameters. The investigations were carried out in spring at four localities along the Skrapez and Crnica rivers: one upstream (the control localities), and three downstream from the fish farm outlets. On the Skrapez River, the fish farm was clearly visible and was markedly changed by the chemical parameters of the water, manifested as a decreased concentration of dissolved oxygen, increased concentrations of nitrates, nitrites, ammonium ions, total phosphorus and total organic carbon, and increased SOD and GPx activities and decreased GSH concentration in larvae from the first downstream locality as compared to the control locality. On the Crnica River, due to the high values of water flow (around 3 m3/s), effluents from the fish farm had no effect on the chemical parameters of the water or on the tested biomarkers.

scholarly journals Seasonal changes in oxidative stress biomarkers of the snail Viviparus acerosus from the Velika Morava River, Serbia

2012 ◽  
Vol 64 (3) ◽  
pp. 953-962 ◽  
Author(s):  
Svetlana Despotovic ◽  
Branka Perendija ◽  
Jelena Gavric ◽  
Slavica Borkovic-Mitic ◽  
M.M. Paunovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Phase Ii ◽  
Seasonal Changes ◽  
Important Variable ◽  
Whole Body ◽  
Glutathione S Transferase ◽  
Total Glutathione ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Morava River

The river snail (Viviparus acerosus) from the Velika Morava River, Serbia was chosen in our study in order to determine seasonal changes in oxidative stress biomarkers between July (summer) and September (autumn). The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and the phase II biotransformation enzyme glutathione-S-transferase (GST), as well as the concentration of total glutathione (GSH), were examined in the whole body of the river snails. The obtained results show significantly higher activities of CAT, GSH-Px, GR and biotransformation phase II enzyme GST in September compared to July, while the GSH concentration was lower. There was no general trend in the seasonal changes in the activity of SOD. The presented data show that animals in their natural environment are exposed to constant fluctuations of environmental conditions that could cause antioxidants to exhibit seasonal variations. This fact should be considered as an important variable in the interpretation of results in biomonitoring studies.

Biomarkers of oxidative stress and tissue damage released by muscle and liver after a single bout of swimming exercise

2013 ◽  
Vol 38 (5) ◽  
pp. 507-511 ◽  
Author(s):  
Dionizio Ramos ◽  
Eduarda Gabrielle Martins ◽  
Diego Viana-Gomes ◽  
Gustavo Casimiro-Lopes ◽  
Verônica P. Salerno
Keyword(s):  
Oxidative Stress ◽  
Acute Exercise ◽  
Cellular Damage ◽  
Swimming Exercise ◽  
Antioxidant Systems ◽  
Inadequate Response ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Single Bout ◽  
Biomarkers Of Oxidative Stress

Both acute exercise and excessive training can cause oxidative stress. The resulting increase in free radicals and the inadequate response from antioxidant systems can lead to a framework of cellular damage. An association between affected tissue and the biomarkers of oxidative stress that appear in plasma has not been clearly established. The aim of this study was to evaluate the source of oxidative stress biomarkers found in the plasma of untrained rats after a single bout of swimming exercise at 2 different intensities: low intensity (SBLIE) or high intensity (SBHIE). Immediately after the exercise, aspartate transaminase (AST), alanine transaminase (ALT), γ-glutamyltransferase (GGT), and lactate dehydrogenase (LDH) were measured in plasma to characterize cell damage. Oxidative stress was assessed using protein carbonylation (PC), total antioxidant capacity (TAC), and thiobarbituric acid reactive substances (TBARS) quantified by malondialdehyde concentration. SBHIE raised levels of plasma AST (93%) and ALT (17%), and both exercise regimens produced an increase in GGT (7%) and LDH (∼55%). Plasma levels of PC and TBARS were greater in the SBHIE group; there were no changes in TAC. SBLIE caused only a modest increase in TBARS. In muscle, there were no changes in TAC, PC, or TBARS, regardless of exercise intensity, In the liver, TAC and TBARS increased significantly in both the SBLIE and SBHIE groups. This indicates that the oxidative stress biomarkers measured in the plasma immediately after a single bout of swimming exercise were generated primarily in the liver, not in muscle.

scholarly journals Reliability and Usefulness of Different Biomarkers of Oxidative Stress in Chronic Obstructive Pulmonary Disease

2020 ◽  
Vol 2020 ◽  
pp. 1-12 ◽  
Author(s):  
Elisabetta Zinellu ◽  
Angelo Zinellu ◽  
Alessandro G. Fois ◽  
Sara S. Fois ◽  
Barbara Piras ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Chronic Obstructive Pulmonary Disease ◽  
Pulmonary Disease ◽  
Analytical Methods ◽  
Airflow Limitation ◽  
Chronic Obstructive ◽  
Oxidative Stress Biomarkers ◽  
Obstructive Pulmonary Disease ◽  
Stress Biomarkers ◽  
Biomarkers Of Oxidative Stress

Introduction. Chronic obstructive pulmonary disease (COPD) is a progressive disease characterized by airflow limitation that is not fully reversible after inhaled bronchodilator use associated with an abnormal inflammatory condition. The biggest risk factor for COPD is cigarette smoking. The exposure to noxious chemicals contained within tobacco smoke is known to cause airway epithelial injury through oxidative stress, which in turn has the ability to elicit an inflammatory response. In fact, the disruption of the delicate balance between oxidant and antioxidant defenses leads to an oxidative burden that has long been held responsible to play a pivotal role in the pathogenesis of COPD. There are currently several biomarkers of oxidative stress in COPD that have been evaluated in a variety of biological samples. The aim of this review is to identify the best studied molecules by summarizing the key literature findings, thus shedding some light on the subject. Methods. We searched for relevant case-control studies examining oxidative stress biomarkers in stable COPD, taking into account the analytical method of detection as an influence factor. Results. Many oxidative stress biomarkers have been evaluated in several biological matrices, mostly in the blood. Some of them consistently differ between the cases and controls even when allowing different analytical methods of detection. Conclusions. The present review provides an overview of the oxidative stress biomarkers that have been evaluated in patients with COPD, bringing focus on those molecules whose reliability has been confirmed by the use of different analytical methods.

scholarly journals Urinary Oxidative Stress Biomarkers in Workers of a Titanium Dioxide Based Pigment Production Plant

2020 ◽  
Vol 17 (23) ◽  
pp. 9085 ◽  
Author(s):  
Flavia Buonaurio ◽  
Maria Luisa Astolfi ◽  
Silvia Canepari ◽  
Marco Di Basilio ◽  
Rocco Gibilras ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Titanium Dioxide ◽  
Geographical Area ◽  
Urine Samples ◽  
International Agency ◽  
Average Particle ◽  
Production Plant ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Biomarkers Of Oxidative Stress

Titanium dioxide is produced or imported into the EU for over one million tons/year. The International Agency for Research on Cancer (IARC) classification is 2B, a possible inhalation carcinogen for humans. This study evaluates urinary biomarkers of oxidative stress in workers of a plant producing TiO2 pigment powder, having 0.25 µm average particle size and an ultrafine fraction, compared to unexposed subjects. Urine samples were collected from forty workers before and after the shift, from six employees of the same company and eighteen volunteers from the same geographical area. Titanium and other metals concentrations were measured by ICP-MS, while DNA, RNA, and protein oxidation products by HPLC/MS-MS. A statistically significant increase was found for the urinary concentration of Al, Cd, Cr, Cu, Fe, Mn, Pb, Ti, and Zr, and for all biomarkers of oxidative stress in post-shift workers’ urine samples. Urinary concentrations after the working shift were higher than for employees and volunteers pooled together for Cd, Mn, and Zr, and for the oxidative stress biomarkers 8-oxoGuo, 8-oxodGuo, and 3NO2Tyr. Biomonitoring studies on dose and effect biomarkers for TiO2 occupational exposure provide information useful for protecting workers’ health even in conditions that comply with health and safety standards, highlighting reversible effects of chronic exposure at very low doses.

scholarly journals Dichloroacetate Decreases Cell Health and Activates Oxidative Stress Defense Pathways in Rat Alveolar Type II Pneumocytes

2015 ◽  
Vol 2015 ◽  
pp. 1-6
Author(s):  
Alexis Valauri-Orton ◽  
Frizzi Bschorer ◽  
Karen K. Bernd
Keyword(s):  
Oxidative Stress ◽  
Alveolar Type ◽  
Type Ii ◽  
Total Glutathione ◽  
Oxidative Stress Biomarkers ◽  
Redox Ratio ◽  
Stress Biomarkers ◽  
Oxidative Stress Defense ◽  
Type Ii Pneumocytes ◽  
Stress Defense

Dichloroacetate (DCA) is a water purification byproduct that is known to be hepatotoxic and hepatocarcinogenic and to induce peripheral neuropathy and damage macrophages. This study characterizes the effects of the haloacetate on lung cells by exposing rat alveolar type II (L2) cells to 0–24 mM DCA for 6–24 hours. Increasing DCA concentration and the combination of increasing DCA concentration plus longer exposures decrease measures of cellular health. Length of exposure has no effect on oxidative stress biomarkers, glutathione, SOD, or CAT. Increasing DCA concentration alone does not affect total glutathione or its redox ratio but does increase activity in the SOD/CAT oxidative stress defense pathway. These data suggest that alveolar type II cells rely on SOD and CAT more than glutathione to combat DCA-induced stress.

Abstract P295: Oxidative Stress Biomarkers and Incidence of Post-Operative Atrial Fibrillation in the OPERA Trial

Circulation ◽  
2015 ◽  
Vol 131 (suppl_1) ◽  
Author(s):  
Jason H Wu ◽  
Roberto Marchioli ◽  
Roberto Latini ◽  
Serge Masson ◽  
Maria G Silletta ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Atrial Fibrillation ◽  
Cardiac Surgery ◽  
Animal Studies ◽  
Urine Level ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Post Operative Atrial Fibrillation ◽  
Restricted Cubic Splines ◽  
Biomarkers Of Oxidative Stress

Background: Post-operative atrial fibrillation (PoAF) commonly complicates cardiac surgery. Animal studies point to oxidative stress as a key mechanism triggering PoAF, and yet, the extent to which oxidative stress might relate to PoAF risk in humans remains speculative. Methods and Results: We assessed the association of validated oxidative stress biomarkers (F 2 -Isoprostanes, F 2 -IsoP, Isofurans, IsoF, and F 3 -Isoprostanes, F 3 -IsoP) in plasma and urine, with incident PoAF among 551 cardiac surgery patients. Biomarkers were measured using standardized methods at baseline, the end of surgery, and post-op day 2. PoAF lasting ≥ 30seconds was confirmed by rhythm strip or ECG, and centrally adjudicated. Outcomes were assessed until hospital discharge or post-operative day 10, whichever occurred first. Urine level of each oxidative stress biomarkers demonstrated a rise at the end of surgery (2-3 fold over baseline, P <0.001), which subsequently fell and were comparable to baseline by post-op day 2. In contrast, plasma levels remained relatively stable throughout. Urine F 2 -iso and IsoF at the end of surgery were 20% and 50% higher in subjects who developed PoAF ( P ≤ 0.009). Baseline biomarker levels did not associate significantly with PoAF, whereas end of surgery and post-op day 2 Isop and IsoF did associate with PoAF. For example, the end of surgery extreme quartile multivariate adjusted odds ratio (95% CI) for urine IsoF and F 3 -IsoP were 1.95 (1.05-3.62; P for trend = 0.01) and 2.10 (1.04-2.25, P for trend = 0.04), respectively. Analyses using restricted cubic splines suggested continuous (monotonic) associations of biomarkers of oxidative stress at the end of surgery and post-op day 2 with incident PoAF ( Figure ) There was little evidence that associations of IsoP and IsoF with PoAF varied by demographic, surgery type, and medication use ( P ≥ 0.29 for each). Conclusions: Elevated end of surgery and post-op day 2 oxidative stress as assessed by IsoP and IsoF relate independently to higher risk of PoAF.

scholarly journals Biomarkers of Oxidative Stress in Normal and Pre-eclamptic Women

KYAMC Journal ◽  
2017 ◽  
Vol 4 (2) ◽  
pp. 406-408
Author(s):  
Shahin Ara ◽  
Taslima Nigar ◽  
Md Anwar Habib
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Pregnant Women ◽  
Control Group ◽  
Third Trimester ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Observational Group ◽  
High Uric Acid ◽  
Biomarkers Of Oxidative Stress

Preeclampsia is a serious pregnancy complication. Little is known about which clinical risk factors are associated with the progression from normal to preeclampsia. Recent evidence suggests that the oxidative stress is one of the important factor associated with preeclampsia. High uric acid and MDA levels are indicative of oxidative stress. The purpose of this study was to evaluate the possible relationship between the biochemical markers (Uric acid & MDA) of oxidative stress in preeclamptic & normal pregnant women. In our study we investigated total number of 40 healthy pregnant and clinically diagnosed preeclamtic women. Among them 20 healthy (third trimester) normal pregnant women were taken as control group and another 20 clinically diagnosed PET women (at pre labour state) were taken as observational group. MDA and uric acid level were within normal range (2.63 ± 0.66 & 337.88 ± 16.52 ?mol/l ) respectively in normal pregnant women but significantly higher (3.74 ± 1.45 & 428.50 ± 23.65 ?mol/l ) in the group of preeclamptic women. This study review our current understanding of oxidative stress biomarkers ( Uric acid & MDA ) in preeclampsia and highlights that increased MDA and Uric acid levels are associated with preeclampsia.KYAMC Journal Vol. 4, No.-2, Jan 2014, Page 406-408

scholarly journals Oxidative Stress Biomarkers in Urine of Metal Carpentry Workers Can Be Diagnostic for Occupational Exposure to Low Level of Welding Fumes from Associated Metals

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3167
Author(s):  
Flavia Buonaurio ◽  
Maria Luisa Astolfi ◽  
Daniela Pigini ◽  
Giovanna Tranfo ◽  
Silvia Canepari ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Occupational Exposure ◽  
Oxidation Products ◽  
Control Group ◽  
Welding Fumes ◽  
Oxidative Stress Biomarkers ◽  
Limit Values ◽  
Stress Biomarkers ◽  
Specificity And Sensitivity ◽  
The Impact

Urinary concentrations of 16 different exposure biomarkers to metals were determined at the beginning and at the end of a working shift on a group of workers in the metal carpentry industry. Five different oxidative stress biomarkers were also measured, such as the oxidation products of RNA and DNA metabolized and excreted in the urine. The results of workers exposed to metals were compared to those of a control group. The metal concentrations found in these workers were well below the occupational exposure limit values and exceeded the mean concentrations of the same metals in the urine of the control group by a factor of four at maximum. Barium (Ba), mercury (Hg), lead (Pb) and strontium (Sr) were correlated with the RNA oxidative stress biomarker, 8-oxo-7, 8-dihydroguanosine (8-oxoGuo), which was found able to discriminate exposed workers from controls with a high level of specificity and sensitivity. The power of this early diagnostic technique was assessed by means of the ROC curve. Ba, rubidium (Rb), Sr, tellurium (Te), and vanadium (V) were correlated with the level of the protein oxidation biomarker 3-Nitrotyrosine (3-NO2Tyr), and Ba, beryllium (Be), copper (Cu), and Rb with 5-methylcytidine (5-MeCyt), an epigenetic marker of RNA damage. These effect biomarkers can help in identifying those workers that can be defined as “occupationally exposed” even at low exposure levels, and they can provide information about the impact that such doses have on their health.

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