scholarly journals Dichloroacetate Decreases Cell Health and Activates Oxidative Stress Defense Pathways in Rat Alveolar Type II Pneumocytes

2015 ◽  
Vol 2015 ◽  
pp. 1-6
Author(s):  
Alexis Valauri-Orton ◽  
Frizzi Bschorer ◽  
Karen K. Bernd
Keyword(s):  
Oxidative Stress ◽  
Alveolar Type ◽  
Type Ii ◽  
Total Glutathione ◽  
Oxidative Stress Biomarkers ◽  
Redox Ratio ◽  
Stress Biomarkers ◽  
Oxidative Stress Defense ◽  
Type Ii Pneumocytes ◽  
Stress Defense

Dichloroacetate (DCA) is a water purification byproduct that is known to be hepatotoxic and hepatocarcinogenic and to induce peripheral neuropathy and damage macrophages. This study characterizes the effects of the haloacetate on lung cells by exposing rat alveolar type II (L2) cells to 0–24 mM DCA for 6–24 hours. Increasing DCA concentration and the combination of increasing DCA concentration plus longer exposures decrease measures of cellular health. Length of exposure has no effect on oxidative stress biomarkers, glutathione, SOD, or CAT. Increasing DCA concentration alone does not affect total glutathione or its redox ratio but does increase activity in the SOD/CAT oxidative stress defense pathway. These data suggest that alveolar type II cells rely on SOD and CAT more than glutathione to combat DCA-induced stress.

scholarly journals Seasonal changes in oxidative stress biomarkers of the snail Viviparus acerosus from the Velika Morava River, Serbia

2012 ◽  
Vol 64 (3) ◽  
pp. 953-962 ◽  
Author(s):  
Svetlana Despotovic ◽  
Branka Perendija ◽  
Jelena Gavric ◽  
Slavica Borkovic-Mitic ◽  
M.M. Paunovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Phase Ii ◽  
Seasonal Changes ◽  
Important Variable ◽  
Whole Body ◽  
Glutathione S Transferase ◽  
Total Glutathione ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Morava River

The river snail (Viviparus acerosus) from the Velika Morava River, Serbia was chosen in our study in order to determine seasonal changes in oxidative stress biomarkers between July (summer) and September (autumn). The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and the phase II biotransformation enzyme glutathione-S-transferase (GST), as well as the concentration of total glutathione (GSH), were examined in the whole body of the river snails. The obtained results show significantly higher activities of CAT, GSH-Px, GR and biotransformation phase II enzyme GST in September compared to July, while the GSH concentration was lower. There was no general trend in the seasonal changes in the activity of SOD. The presented data show that animals in their natural environment are exposed to constant fluctuations of environmental conditions that could cause antioxidants to exhibit seasonal variations. This fact should be considered as an important variable in the interpretation of results in biomonitoring studies.

scholarly journals Influence of trout farm effluents on selected oxidative stress biomarkers in larvae of Ecdyonurus venosus (Ephemeroptera, Heptageniidae)

2019 ◽  
Vol 71 (2) ◽  
pp. 225-233
Author(s):  
Andjelina Radojevic ◽  
Dejan Mircic ◽  
Miroslav Zivic ◽  
Vesna Peric-Mataruga ◽  
Milenka Bozanic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Fish Farm ◽  
Chemical Parameters ◽  
Ammonium Ions ◽  
Total Glutathione ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Trout Farms ◽  
Physical And Chemical ◽  
Biomarkers Of Oxidative Stress

The aim of the present study was to establish the extent to which the outlet waters of trout farms affect the ecosystems of the Crnica and Skrapez rivers in Serbia. We monitored selected biomarkers of oxidative stress: superoxide dismutase (SOD), glutathione peroxidase (GPx) and total glutathione (GSH) in larvae of the species Ecdyonurus venosus, and simultaneously analyzed the changes in the physical and chemical parameters. The investigations were carried out in spring at four localities along the Skrapez and Crnica rivers: one upstream (the control localities), and three downstream from the fish farm outlets. On the Skrapez River, the fish farm was clearly visible and was markedly changed by the chemical parameters of the water, manifested as a decreased concentration of dissolved oxygen, increased concentrations of nitrates, nitrites, ammonium ions, total phosphorus and total organic carbon, and increased SOD and GPx activities and decreased GSH concentration in larvae from the first downstream locality as compared to the control locality. On the Crnica River, due to the high values of water flow (around 3 m3/s), effluents from the fish farm had no effect on the chemical parameters of the water or on the tested biomarkers.

scholarly journals Oxidative Stress Biomarkers in Urine of Metal Carpentry Workers Can Be Diagnostic for Occupational Exposure to Low Level of Welding Fumes from Associated Metals

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3167
Author(s):  
Flavia Buonaurio ◽  
Maria Luisa Astolfi ◽  
Daniela Pigini ◽  
Giovanna Tranfo ◽  
Silvia Canepari ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Occupational Exposure ◽  
Oxidation Products ◽  
Control Group ◽  
Welding Fumes ◽  
Oxidative Stress Biomarkers ◽  
Limit Values ◽  
Stress Biomarkers ◽  
Specificity And Sensitivity ◽  
The Impact

Urinary concentrations of 16 different exposure biomarkers to metals were determined at the beginning and at the end of a working shift on a group of workers in the metal carpentry industry. Five different oxidative stress biomarkers were also measured, such as the oxidation products of RNA and DNA metabolized and excreted in the urine. The results of workers exposed to metals were compared to those of a control group. The metal concentrations found in these workers were well below the occupational exposure limit values and exceeded the mean concentrations of the same metals in the urine of the control group by a factor of four at maximum. Barium (Ba), mercury (Hg), lead (Pb) and strontium (Sr) were correlated with the RNA oxidative stress biomarker, 8-oxo-7, 8-dihydroguanosine (8-oxoGuo), which was found able to discriminate exposed workers from controls with a high level of specificity and sensitivity. The power of this early diagnostic technique was assessed by means of the ROC curve. Ba, rubidium (Rb), Sr, tellurium (Te), and vanadium (V) were correlated with the level of the protein oxidation biomarker 3-Nitrotyrosine (3-NO2Tyr), and Ba, beryllium (Be), copper (Cu), and Rb with 5-methylcytidine (5-MeCyt), an epigenetic marker of RNA damage. These effect biomarkers can help in identifying those workers that can be defined as “occupationally exposed” even at low exposure levels, and they can provide information about the impact that such doses have on their health.

scholarly journals Antioxidants, lysosomes and elements status during the life cycle of sea trout Salmo trutta m. trutta L.

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Natalia Kurhaluk ◽  
Halyna Tkachenko
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Muscle Tissue ◽  
Salmo Trutta ◽  
Protein Carbonyl ◽  
Oxidative Modification ◽  
Oxidative Stress Biomarkers ◽  
Development Stages ◽  
Stress Biomarkers ◽  
Element Contents

AbstractThe aim of our study was to elucidate the effects of both development stages (parr, smolt, adult, spawner), and kelt as a survival form and sex (male, female) on the functional stability of the lysosomal complex, biomarkers of oxidative stress, and element contents in the muscle tissue of the sea trout (Salmo trutta m. trutta L.) sampled in the Pomerania region (northern Poland). We have evaluated the maximal activities of lysosomal enzymes (alanyl aminopeptidase, leucyl aminopeptidase, β-N-acetylglucosaminidase, and acid phosphatase), lipid peroxidation level, and protein carbonyl derivatives as indices of muscle tissue degradation. The relationship between lysosomal activity and oxidative stress biomarkers estimated by the lipid peroxidation level and protein carbonyl derivatives was also assessed, as well as the relationships between element levels and oxidative stress biomarkers. Trends of the main effects (i.e., the development stages and sex alone, the interaction of the sex and development stage simultaneously) on oxidative stress biomarkers, lysosomal functioning, and element contents in the muscle tissue were evaluated. The study has shown sex-related relationships between the pro- and antioxidant balance and the tissue type in the adult stage as well as modifications in the lysosomal functioning induced by long-term environmental stress associated with changing the habitats from freshwater to seawater and intense migrations. The highest level of toxic products generated in oxidative reactions and oxidative modification of proteins was noted in both the spawner stage and the kelt form. The holistic model of analysis of all parameters of antioxidant defense in all development stages and sex demonstrated the following dependencies for the level of lipid peroxidation, oxidative modification of proteins, lysosomal activities, and element contents: TBARS > OMP KD > OMP AD > TAC, AcP > NAG > LAP > AAP and Cu > Fe > Ca > Mn > Zn > Mg, respectively.

Could resistant starch supplementation improve inflammatory and oxidative stress biomarkers and uremic toxins levels in hemodialysis patients? A pilot randomized controlled trial

2018 ◽  
Vol 9 (12) ◽  
pp. 6508-6516 ◽  
Author(s):  
Marta Esgalhado ◽  
Julie A. Kemp ◽  
Renata Azevedo ◽  
Bruna R. Paiva ◽  
Milena B. Stockler-Pinto ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Resistant Starch ◽  
Controlled Trial ◽  
Hemodialysis Patients ◽  
Uremic Toxins ◽  
Oxidative Stress Biomarkers ◽  
Randomized Controlled ◽  
Stress Biomarkers ◽  
And Oxidative Stress ◽  
Pilot Randomized Controlled Trial

Prebiotic-resistant starch supplementation may be a good strategy to reduce inflammation, oxidative stress and uremic toxins in CKD patients.

Ameliorative Effects of Resveratrol on Oxidative Stress Biomarkers in Horses

2015 ◽  
Vol 35 (6) ◽  
pp. 518-523 ◽  
Author(s):  
Mary Ucheagha Ememe ◽  
Wayuta Philip Mshelia ◽  
Joseph Olusegun Ayo
Keyword(s):  
Oxidative Stress ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers
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