scholarly journals Cytotoxicity and genotoxicity of iron oxide nanoparticles: An in vitro biosafety study

2016 ◽  
Vol 68 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Erdal Sonmez ◽  
Elanur Aydin ◽  
Hasan Turkez ◽  
Elvan Özbek ◽  
Basak Togar ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Oxidative Damage ◽  
Cell Viability ◽  
Iron Oxide Nanoparticles ◽  
Human Blood ◽  
Blood Cells ◽  
Biological Effects ◽  
Oxide Nanoparticles ◽  
Human Blood Cells ◽  
Dose Dependent

With the development of nanotechnology and the wide use of iron oxide nanoparticles, it has become necessary to assess the potential adverse biological effects of magnetite. This study investigated the cytotoxicity, genotoxicity and oxidative damage of different concentrations of magnetite (0 to 1000 mg/L) in human whole blood cultures. After supplementation of magnetite, the blood samples were incubated for 72 h. Cell viability was assessed by the 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays. The total antioxidant capacity (TAC) and total oxidant status (TOS) were determined to evaluate the dose-dependent effects of magnetite on the oxidant/antioxidant balance and to evaluate the potential oxidative injury due to increased oxidative stress. Genotoxicity was estimated by by the sister chromatid exchange (SCE), micronuclei (MN) and chromosome aberration (CA) assays and determination of 8-oxo-2-deoxyguanosine (8-OH-dG) levels. The results of MTT and LDH assays showed that the higher concentrations of magnetite (100, 150, 300, 500 and 1000 mg/L) decreased cell viability. Concentrations of magnetite higher than 10 mg/L increased TOS levels and decreased TAC levels in human blood cells. Increasing concentrations of magnetite caused significant increases in MN, SCE and CA rates and 8-OH-dG levels. The obtained results showed that magnetite exerted dose-dependent effects on oxidative damage, genotoxicity and cytotoxicity in human blood cells.

Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro

2017 ◽  
Vol 69 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Saša Lazović ◽  
Andreja Leskovac ◽  
Sandra Petrović ◽  
Lidija Senerovic ◽  
Nevena Krivokapić ◽  
...  
Keyword(s):  
Human Blood ◽  
Blood Cells ◽  
Biological Effects ◽  
Gas Plasma ◽  
Human Blood Cells ◽  
Bacterial Pigment

Investigations of Peptide-Based Biocompatible Injectable Shape-Memory Hydrogels: Differential Biological Effects on Bacterial and Human Blood Cells

2018 ◽  
Vol 10 (13) ◽  
pp. 10729-10740 ◽  
Author(s):  
Pramod K. Gavel ◽  
Dharm Dev ◽  
Hamendra S. Parmar ◽  
Sheetal Bhasin ◽  
Apurba K. Das
Keyword(s):  
Shape Memory ◽  
Human Blood ◽  
Blood Cells ◽  
Biological Effects ◽  
Human Blood Cells

Magnetic resonance imaging/fluorescence dual modality protocol using designed phosphonate ligands coupled to superparamagnetic iron oxide nanoparticles

2016 ◽  
Vol 4 (22) ◽  
pp. 3969-3981 ◽  
Author(s):  
Tina Lam ◽  
Pramod K. Avti ◽  
Philippe Pouliot ◽  
Jean-Claude Tardif ◽  
Éric Rhéaume ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Iron Oxide ◽  
Magnetic Resonance ◽  
Cell Viability ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Oxide Nanoparticles ◽  
Resonance Imaging ◽  
Phosphonate Ligands ◽  
Very High

Easily dispersed in water and fluorescent. Very high r2 and r2* relaxivities. Dye does not influence cell viability of the probe.

scholarly journals Cellular Toxicity Mechanisms and the Role of Autophagy in Pt(IV) Prodrug-Loaded Ultrasmall Iron Oxide Nanoparticles Used for Enhanced Drug Delivery

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1730
Author(s):  
L. Gutiérrez-Romero ◽  
L. Rivas-García ◽  
C. Sánchez-González ◽  
J. Llopis ◽  
E. Blanco ◽  
...  
Keyword(s):  
Cell Death ◽  
Iron Oxide ◽  
Cell Viability ◽  
Iron Oxide Nanoparticles ◽  
Nuclear Dna ◽  
Time Dependent ◽  
Ovarian Cancer Cells ◽  
Oxide Nanoparticles ◽  
Free Media ◽  
Drug Free

Ultrasmall iron oxide nanoparticles (<10 nm) were loaded with cis-diamminetetrachloroplatinum (IV), a cisplatin (II) prodrug, and used as an efficient nanodelivery system in cell models. To gain further insight into their behavior in ovarian cancer cells, the level of cellular incorporation as well as the platination of mitochondrial and nuclear DNA were measured using inductively coupled plasma mass spectrometry (ICP-MS) strategies. Quantitative Pt results revealed that after 24 h exposure to 20 µM Pt in the form of the Pt(IV)-loaded nanoparticles, approximately 10% of the incorporated Pt was associated with nuclear DNA. This concentration increased up to 60% when cells were left to stand in drug-free media for 3 h. These results indicated that the intracellular reducing conditions permitted the slow release of cisplatin (II) from the cisplatin (IV)-loaded nanoparticles. Similar results were obtained for the platination of mitochondrial DNA, which reached levels up to 17,400 ± 75 ng Pt/ mg DNA when cells were left in drug-free media for 3 h, proving that this organelle was also a target for the action of the released cisplatin (II). The time-dependent formation of Pt-DNA adducts could be correlated with the time-dependent decrease in cell viability. Such a decrease in cell viability was correlated with the induction of apoptosis as the main route of cell death. The formation of autophagosomes, although observed upon exposure in treated cells, does not seem to have played an important role as a means for cells to overcome nanoparticles’ toxicity. Thus, the designed nanosystem demonstrated high cellular penetration and the “in situ” production of the intracellularly active cisplatin (II), which is able to induce cell death, in a sustained manner.

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