scholarly journals Correlation between Ki-67 and telomerase expression with in situ hybridization for high-risk human papillomavirus

2013 ◽  
Vol 65 (1) ◽  
pp. 81-90 ◽  
Author(s):  
Vega-Peña Arianna ◽  
Illades-Aguiar Berenice ◽  
Flores-Alfaro Eugenia ◽  
López-Bayghen Esther ◽  
Reyes-Maldonado Elba ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
High Risk ◽  
Ki 67

scholarly journals Significance of p53-Binding Protein 1 Nuclear Foci in Cervical Squamous Intraepithelial Lesions: Association With High-Risk Human Papillomavirus Infection and P16INK4a Expression

2020 ◽  
Vol 27 (1) ◽  
pp. 107327481990117
Author(s):  
Sayaka Kawashita ◽  
Katsuya Matsuda ◽  
Hisayoshi Kondo ◽  
Yuriko Kitajima ◽  
Yuri Hasegawa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
High Risk ◽  
Binding Protein ◽  
Histological Grade ◽  
Neoplastic Progression ◽  
Cervical Lesions ◽  
Nuclear Foci ◽  
The Impact

As p53-binding protein 1 (53BP1) localizes to the sites of DNA double-strand breaks and rapidly forms nuclear foci (NF), and its presence may be an indicator of endogenous genomic instability (GIN). We previously showed that 53BP1 NF in cervical cells increase with neoplastic progression, indicating the significance of 53BP1 expression for the estimation of malignant potential during cervical carcinogenesis. This study aimed to further elucidate the impact of 53BP1 expression as a biomarker for cervical squamous intraepithelial lesion (SIL). A total of 81 tissue samples, including 17 of normal cervical epithelium, 22 of cervical intraepithelial neoplasia (CIN) 1, 21 of CIN2, and 21 of CIN3, from patients positive for high-risk human papillomavirus (HR-HPV) were used for double-label immunofluorescence of 53BP1 and Ki-67/p16INK4a expression and HR-HPV in situ hybridization. We analyzed associations between 53BP1 expression type with parameters such as CIN grade, HR-HPV infection status, p16INK4a expression, and CIN prognosis. Expression type of 53BP1 was significantly associated with histological grade of CIN and HR-HPV in situ hybridization signal pattern ( P < .0001). There was a significant correlation between 53BP1 and p16INK4a expression levels ( r = .73, P < .0001). However, there was no association between 53BP1 expression type and CIN prognosis. We propose that 53BP1 expression type is a valuable biomarker for SIL, which can help estimate the grade and GIN of cervical lesions reflecting replication stress caused by the integration of HR-HPV to the host genome.

scholarly journals Comparison of hybrid capture 2 with in situ hybridization for the detection of high-risk human papillomavirus in liquid-based cervical samples

Cancer ◽  
2003 ◽  
Vol 102 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Albertus T. Hesselink ◽  
Adriaan J. C. van den Brule ◽  
Antoinette A. T. P. Brink ◽  
Johannes Berkhof ◽  
Folkert J. van Kemenade ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
High Risk ◽  
Hybrid Capture ◽  
Hybrid Capture 2

P16 Expression in Extramammary Paget’s Disease of the Vulva and Scrotum Is Not Human Papillomavirus Related

2018 ◽  
Vol 26 (7) ◽  
pp. 617-620 ◽  
Author(s):  
Khaleel I. Al-Obaidy ◽  
Chia-Sui Kao ◽  
Muhammad T. Idrees
Keyword(s):  
Information System ◽  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
High Risk ◽  
Laboratory Information System ◽  
Paget Disease ◽  
Positive Reactivity ◽  
High Risk Hpv ◽  
P16 Expression

Introduction. Extramammary Paget disease (EMPD) of the vulva has been shown to express p16 by immunohistochemistry (IHC), however, p16 expression in the vulva and scrotum has not been extensively studied in relation to human papillomavirus (HPV) within EMPD of both the vulva and scrotum. Design. Twenty-two cases of EMPD (vulva, 16; scrotum, 6) were found in our laboratory information system. P16 and HPV IHC were performed. Any p16 reactivity less than 10% was considered negative. HPV in situ hybridization for both low- and high-risk HPV was also performed on all cases. Results. Of the 6 scrotal EMPD, 3 (50%) showed weak to moderate positive reactivity for p16 by IHC. Of the 16 vulvar EMPD, 13 (81%) were positive for p16, with at least moderate (2+) intensity with a mean expression of 33.3% (range = 10% to 80%) and 62% (range = 20% to 95%) in scrotal and vulvar EMPD, respectively. None of the scrotal or vulvar cases showed positive reactivity for HPV either by IHC or in situ hybridization. Conclusion. Both vulvar and scrotal EMPD can express p16 by IHC, more commonly vulvar than scrotal; however, no HPV was detected either by IHC or in situ hybridization. EMPD of vulva and scrotum does not appear to be related to HPV, and p16 expression may be regulated through a different mechanism.

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