scholarly journals Comparison of hybrid capture 2 with in situ hybridization for the detection of high-risk human papillomavirus in liquid-based cervical samples

Cancer ◽  
2003 ◽  
Vol 102 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Albertus T. Hesselink ◽  
Adriaan J. C. van den Brule ◽  
Antoinette A. T. P. Brink ◽  
Johannes Berkhof ◽  
Folkert J. van Kemenade ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
High Risk ◽  
Hybrid Capture ◽  
Hybrid Capture 2

Cell Block Preparation versus Liquid-Based Thin-Layer Cervical Cytology: A Comparative Study Evaluating Human Papillomavirus Testing by Hybrid Capture-2/Cervista, in situ Hybridization and p16 Immunohistochemistry

2016 ◽  
Vol 60 (2) ◽  
pp. 145-153 ◽  
Author(s):  
Ossama Tawfik ◽  
Marilyn Davis ◽  
Francisco J. Diaz ◽  
Fang Fan
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Test Performance ◽  
High Sensitivity ◽  
Cell Block ◽  
Hybrid Capture ◽  
P16 Immunohistochemistry ◽  
Therapeutic Decisions ◽  
Hybrid Capture 2

Objective: Cell block (CB) preparations from residual liquid-based Pap samples have been shown to be of diagnostic value. In this study we evaluated human papillomavirus (HPV) in situ hybridization (ISH) and p16 immunohistochemistry (IHC) on CB preparations and compared the results with the primary diagnosis and standard HPV tests. Design: In total, 197 HE-stained CB slides prepared from CBs from residual Pap samples (152 ASCUS, 2 ASC-H, 32 LGSIL, 4 HGSIL, 1 AGUS and 6 normal) were analyzed. Hybrid Capture-2 (HC-2)/Cervista testing and HPV ISH and p16 IHC were performed on the CB samples. The test performance characteristics were compared with HPV and p16 assay performances. Results: The cellular architecture was well maintained in CBs with excellent consistency. HPV ISH testing had an excellent concordance with the HC-2/Cervista methods (85%) with high sensitivity (82.6%; 95% CI 75.9, 89.4) and specificity (89.3%). Of all the p16 tests, 38% were positive (60 out of 159 samples). The overall concordance between p16 and HC-2/Cervista (64%), or between p16 and ISH (68%), was lower than the concordance between ISH and HC-2/Cervista (85%). Conclusions: HPV ISH and p16 IHC testing is feasible, cost effective and practical. A combination of the two tests would ultimately improve diagnostic accuracy, leading to better therapeutic decisions.

scholarly journals Low prevalence of high risk human papillomavirus in normal oral mucosa by hybrid capture 2

2008 ◽  
Vol 39 (1) ◽  
Author(s):  
Maria del Refugio González-Losa ◽  
Luis Manzano-Cabrera ◽  
Florencio Rueda-Gordillo ◽  
Sandra E. Hernández-Solís ◽  
Marylin Puerto-Solís
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Oral Mucosa ◽  
Hybrid Capture ◽  
Normal Oral Mucosa ◽  
Hybrid Capture 2 ◽  
Low Prevalence

Novaprep® Vial Test is a suitable liquid-based cytology medium for high risk human papillomavirus testing by Hybrid Capture 2

2010 ◽  
Vol 49 (4) ◽  
pp. 286-289 ◽  
Author(s):  
Jean-Luc Prétet ◽  
Chrystelle Vidal ◽  
Karine Le Bail Carval ◽  
Rajeev Ramanah ◽  
Xavier Carcopino ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Hybrid Capture ◽  
Human Papillomavirus Testing ◽  
Hybrid Capture 2 ◽  
Liquid Based Cytology

Hybrid Capture-2 is Better Than In Situ Hybridization: A Study Comparison With Cervical Biopsies

2010 ◽  
Vol 18 (2) ◽  
pp. 192-194
Author(s):  
Janice Matthews-Greer ◽  
Gloria Caldito ◽  
Guillermo A. Herrera ◽  
Elba A. Turbat-Herrera
Keyword(s):  
In Situ Hybridization ◽  
Hybrid Capture ◽  
Hybrid Capture 2 ◽  
Better Than

scholarly journals Hybrid Capture 2 is as Effective as PCR Testing for High-risk Human Papillomavirus in Head and Neck Cancers

2015 ◽  
Vol 23 (4) ◽  
pp. 266-272 ◽  
Author(s):  
Jody E. Hooper ◽  
Jessica F. Hebert ◽  
Amy Schilling ◽  
Neil D. Gross ◽  
Joshua S. Schindler ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Head And Neck ◽  
Head And Neck Cancers ◽  
Hybrid Capture ◽  
Hybrid Capture 2
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