scholarly journals Survivin in relation to Bcl-2, Bax and in situ apoptotic cell death in anaplastic thyroid carcinoma

2011 ◽  
Vol 63 (4) ◽  
pp. 955-963
Author(s):  
Sonja Selemetjev ◽  
Dubravka Cvejic ◽  
Svetlana Savin ◽  
I. Paunovic ◽  
S. Tatic
Keyword(s):  
Cell Death ◽  
Thyroid Carcinoma ◽  
Apoptotic Cell ◽  
Anaplastic Thyroid Carcinoma ◽  
Apoptotic Cell Death ◽  
Staining Score ◽  
Human Malignancy ◽  
Apoptotic Pathways ◽  
Individual Scores

Anaplastic thyroid carcinoma (ATC) is a rare but highly aggressive human malignancy. It is known that disturbances in apoptotic pathways have a great impact on tumor progression and aggressiveness. In this study the apoptosisrelated molecules Bcl-2 (antiapoptotic), Bax (proapoptotic) and survivin (an inhibitor of apoptosis) were analyzed immunohistochemically in thirty archival cases of ATC. In situ apoptotic cell death was analyzed by the TUNEL method. Mean Bcl-2 staining score (calculated from individual scores from 0-3) was low compared to those for Bax and survivin (p<0.05). High expression of survivin was associated with high Bax expression, and was significantly segregated from high Bcl-2 expressing cases (p<0.05). Despite high Bax expression, apoptotic cell death was low in the investigated carcinomas. In addition, the mean apoptotic index in high survivin expressing carcinomas was significantly lower than in low survivin expressing carcinomas (p<0.05). It could be concluded that down-regulation of Bcl-2 is counterbalanced by up-regulation of survivin, which may overcome the effects of high Bax expression, and, at least partly, explain the low apoptosis rate and high biological aggressiveness of ATC.

Valproic Acid Sensitizes TRAIL-Resistant Anaplastic Thyroid Carcinoma Cells to Apoptotic Cell Death

2013 ◽  
Vol 20 (S3) ◽  
pp. 716-724 ◽  
Author(s):  
Hyun-Young Cha ◽  
Bok-Soon Lee ◽  
Sam Kang ◽  
Yoo Seob Shin ◽  
Jae Won Chang ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Cell Death ◽  
Thyroid Carcinoma ◽  
Apoptotic Cell ◽  
Anaplastic Thyroid Carcinoma ◽  
Apoptotic Cell Death ◽  
Carcinoma Cells

In situ labeling of apoptotic cell death in the cerebral cortex and thalamus of rats during development

1995 ◽  
Vol 363 (2) ◽  
pp. 281-295 ◽  
Author(s):  
Roberto Spreafico ◽  
Carolina Frassoni ◽  
Paola Arcelli ◽  
Mariateresa Selvaggio ◽  
Silvia De Biasi
Keyword(s):  
Cell Death ◽  
Cerebral Cortex ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death

Apoptotic cell death induction and angiogenesis inhibition in large established medullary thyroid carcinoma xenografts by Ret inhibitor RPI-1

2006 ◽  
Vol 72 (4) ◽  
pp. 405-414 ◽  
Author(s):  
Giovanna Petrangolini ◽  
Giuditta Cuccuru ◽  
Cinzia Lanzi ◽  
Monica Tortoreto ◽  
Sara Belluco ◽  
...  
Keyword(s):  
Cell Death ◽  
Thyroid Carcinoma ◽  
Medullary Thyroid Carcinoma ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Medullary Thyroid ◽  
Angiogenesis Inhibition

scholarly journals Apoptotic cell death in the porcine endometrium during the oestrous cycle

2001 ◽  
Vol 49 (1) ◽  
pp. 71-79 ◽  
Author(s):  
Barbara Wąsowska ◽  
Beata Ludkiewicz ◽  
Stanisława Stefańczyk-Krzymowska ◽  
W. Grzegorzewski ◽  
Janina Skipor
Keyword(s):  
Cell Death ◽  
Stromal Cells ◽  
Cell Kinetics ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Oestrous Cycle ◽  
Apoptotic Cells ◽  
Early Follicular Phase ◽  
Labelling Method

It has been reported that apoptosis plays an essential role in controlling the physiological cell kinetics in the human and rodent endometrium but this type of death has never been studied in the porcine endometrium. The aim of this study was to investigate the apoptotic cell death in the porcine endometrium during the middle (Days 9–11) and late (Day 13) luteal phase, during the luteolysis (Day 15) and early follicular phase (Days 17–19) of the oestrous cycle. Apoptotic cells were identified by in situ DNA 3′-end labelling method. it was revealed that the greatest number of apoptotic cells in the luminal and glandular epithelium was found on Days 17–19 and on Day 15 of the oestrous cycle, respectively. in the stroma, the greatest number of these cells was found on Days 9–11. Our data have shown that in the porcine endometrium, both epithelial and stromal cells undergo apoptosis and that the number of apoptotic cells varies depending on the phase of the oestrous cycle.

Detection of apoptotic cell death in the thymus of dexamethasone treated rats using [123I]Annexin V and in situ oligonucleotide ligation

2007 ◽  
Vol 38 (4) ◽  
pp. 313-319 ◽  
Author(s):  
Katerina Zavitsanou ◽  
Vu Nguyen ◽  
Ivan Greguric ◽  
Janette Chapman ◽  
Patrice Ballantyne ◽  
...  
Keyword(s):  
Cell Death ◽  
Apoptotic Cell ◽  
Annexin V ◽  
Apoptotic Cell Death

scholarly journals Apoptotic cell death at tooth eruption in rat molar observed by in situ labeling of nuclear DNA fragmentation.

1995 ◽  
Vol 37 (6) ◽  
pp. 488-492 ◽  
Author(s):  
Yoshihiro Abiko ◽  
Hidetoshi Kanno ◽  
Jiro Arai ◽  
Michiko Nishimura ◽  
Masato Saitoh ◽  
...  
Keyword(s):  
Cell Death ◽  
Dna Fragmentation ◽  
Nuclear Dna ◽  
Apoptotic Cell ◽  
Tooth Eruption ◽  
Apoptotic Cell Death ◽  
Nuclear Dna Fragmentation ◽  
Rat Molar

PERP, a p53 proapoptotic target, mediates apoptotic cell death in renal ischemia

2009 ◽  
Vol 296 (4) ◽  
pp. F847-F858 ◽  
Author(s):  
Kurinji Singaravelu ◽  
Kishor Devalaraja-Narashimha ◽  
Brynn Lastovica ◽  
Babu J. Padanilam
Keyword(s):  
Cell Death ◽  
Cytochrome C ◽  
Ischemia Reperfusion Injury ◽  
Apoptotic Cell ◽  
Ischemia Reperfusion ◽  
Suppressor Gene ◽  
Apoptotic Cell Death ◽  
Renal Ischemia ◽  
Dependent Manner ◽  
Apoptotic Pathways

The p53 tumor suppressor gene plays a crucial role in mediating apoptotic cell death in renal ischemia-reperfusion injury (IRI). To further elucidate the p53-dependent pathway, we investigated the role of the p53 apoptosis effector related to PMP-22 (PERP), an apoptosis-associated p53 transcriptional target. PERP mRNA and protein are highly induced in the outer medullary proximal tubular cells (PTC) of ischemic kidneys postreperfusion at 3, 12, and 24 h in a p53-dependent manner. In PTC, overexpression of PERP augmented the rate of apoptosis following hypoxia by inducing mitochondrial permeability and subsequent release of cytochrome c, apoptosis-inducing factor (AIF), and caspase 9 activation. In addition, silencing of the PERP gene with short hairpin RNA prevented apoptosis in hypoxia-mediated injury by precluding mitochondrial dysfunction and consequent cytochrome c and AIF translocation. These data suggest that PERP is a key effector of p53-mediated apoptotic pathways and is a potential therapeutic target for renal IRI.

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