scholarly journals A mini review: RuBisCo small subunit as a strong, green tissue-specific promoter

2011 ◽  
Vol 63 (2) ◽  
pp. 299-307 ◽  
Author(s):  
Allah Bakhsh ◽  
Rao Qayyum ◽  
Zeeshan Shamim ◽  
Tayyab Husnain
Keyword(s):  
Gene Expression ◽  
Transgenic Plants ◽  
Genetic Engineering ◽  
Transgene Expression ◽  
Small Subunit ◽  
Tissue Specific ◽  
Rubisco Small Subunit ◽  
Green Tissue ◽  
Study Gene Expression ◽  
Tissue Specific Promoter

Plant genetic transformation is a powerful application used to study gene expression in plants. Transcriptomics has the potential to rapidly increase our knowledge of spatial and temporal gene expression and lead to new promoters for research and development. The availability of a broad spectrum of promoters with the ability to regulate the temporal and spatial expression patterns of transgenes can increase the successful application of transgenic technology. A variety of promoters is necessary at all levels of genetic engineering in plants, from basic research, to the development of economically viable crops and plant commodities, it can address legitimate concerns raised about the safety and containment of transgenic plants in the environment. Compared with temporal- or spatial-specific expression of a toxin, constitutive expression of foreign proteins in transgenic plants can cause adverse effects. The constitutive overexpression of transgenes that interferes with normal processes in a plant underscores the need for refinement of transgene expression. The development of tissue-specific promoters to drive transgene expression has helped fulfill that need. Therefore, in certain circumstances it is desirable to use expression-specific promoters which only express the foreign gene in specific plant tissues or organs. This review highlights the uses and benefits reaped by the use of green tissue-specific promoter for the RuBisCo small subunit in different crops and systems and thus establishing a broad range of tissue-specific promoters. Such plant promoters that are activated precisely when and where they are needed would be ideal for genetic engineering strategies.

scholarly journals Endeavours of RuBisCO small subunit promoter as a tool of green tissue specific expression

2012 ◽  
Vol 48 (No. 1) ◽  
pp. 1-9 ◽  
Author(s):  
A. Bakhsh ◽  
T. Husnain
Keyword(s):  
Transgenic Plants ◽  
Genetic Engineering ◽  
Transgene Expression ◽  
Expression Patterns ◽  
Small Subunit ◽  
Foreign Gene ◽  
Specific Expression ◽  
Tissue Specific ◽  
Rubisco Small Subunit ◽  
Green Tissue

Transcriptomics has the potential to rapidly increase our knowledge of spatial and temporal gene expression and contributes to the characterization of new promoters for research and development. The successful application of transgenic technology has been further strengthened by the availability of a broad spectrum of promoters having the ability to regulate the temporal and spatial expression patterns of the transgene. A variety of promoters is necessary at all levels of genetic engineering in plants, from basic research discoveries, to development of economically viable crops and plant commodities, to addressing legitimate concerns raised about the safety and containment of transgenic plants in the environment. Compared with the temporal- or spatial-specific expression of the toxin, constitutive expression of foreign proteins in transgenic plants may cause adverse effects. Constitutive overexpression of transgenes that interfere with normal processes in a plant underscores the need for refinement of transgene expression. The development of tissue-specific promoters to drive transgene expression has helped us to fulfil that need. Therefore, in certain circumstances, it is desirable to use expression-specific promoters which express only the foreign gene in specific plant tissues or organs. This review highlights the uses and benefits reaped by researchers by using a green tissue specific promoter, RuBisCO small subunit promoter, in different crops and systems and thus establishing a broad range of tissue specific promoters. Such plant promoters that are activated precisely when and where they are needed would be ideal for genetic engineering strategies.

RuBisCo small subunit as strong green tissue specific promoter

2011 ◽  
Vol 22 ◽  
pp. S139
Author(s):  
Faheem Shahzad Baloch ◽  
Allah Bakhsh ◽  
Muhammad Asim
Keyword(s):  
Small Subunit ◽  
Tissue Specific ◽  
Rubisco Small Subunit ◽  
Green Tissue ◽  
Tissue Specific Promoter

scholarly journals Expression of erythropoietin in Indian tetraploid potato variety

F1000Research ◽  
2012 ◽  
Vol 1 ◽  
pp. 26
Author(s):  
Priti N Desai ◽  
Harish Padh
Keyword(s):  
Transgenic Plants ◽  
Mammalian Cells ◽  
Capital Investment ◽  
Transgene Expression ◽  
Present Report ◽  
Expression System ◽  
Expression Vectors ◽  
Pcr Analysis ◽  
Tissue Specific ◽  
Tissue Specific Promoter

With the advent of protein-based biotech drugs in the market, the quest for the “perfect” protein expression system, which is both economical and effective, has come into focus. Currently bacteria, yeast, insect cells, mammalian cells, transgenic animal and transgenic plants are widely used for the expression of therapeutic proteins. Among these, transgenic plants provide advantages in terms of low production cost, lower capital investment in infrastructure, and suitable post-translational modifications. The major limitation of plants as an expression host is the low level of transgene expression. To increase the expression of heterologous proteins in plants, a number of approaches have been used. One of the approaches is to increase the transgene expression by using tissue-specific promoter(s) which can concentrate the protein of interest in targeted tissues and, thus, prove advantageous in downstream purification. In the present report, a protocol for expression of heterologous protein erythropoietin in potato tuber using patatin, the tuber-tissue-specific promoter, was standardized. Expression vectors for production of the erythropoietin gene under tissue-specific promoter were successfully constructed. For production of a transgenic plant, tissue culture techniques for regeneration of the whole plant from single explants were standardized. Polymerase chain reaction (PCR) analysis was performed to confirm the stable integration of the erythropoietin gene in the potato plant by using sequence-specific primers.

Approaches to improve heterogeneous gene expression in transgenic plants

2006 ◽  
Vol 3 (2) ◽  
pp. 75-81 ◽  
Author(s):  
Yin Tao ◽  
Zhang Shang-long ◽  
Liu Jing-mei ◽  
Chen De-ming
Keyword(s):  
Gene Expression ◽  
Transgenic Plants ◽  
Genetic Engineering ◽  
Transgenic Plant ◽  
Transgene Expression ◽  
Dna Modification ◽  
Plant Genetic Engineering ◽  
Engineering Research ◽  
Low Efficiency ◽  
Transformation Methods

AbstractWith the development of plant genetic engineering, many transformation methods can be used to transfer heterogeneous genes into plants to develop genetic crops. However, a lot of research results have shown that transgene expression remains largely unpredictable and there is great variation of expression level in different transgenic plant lines. Plant genetic engineering research is reviewed in the present paper. We analysed the reasons why low efficiency of heterogeneous gene expression has happened in transgenic plants in terms of DNA modification, localization of proteins and methods of transformation used. Some strategies for improving heterogeneous gene expression in transgenic plants are also discussed.

A system for tissue-specific copper-controllable gene expression in transgenic plants: Nodule-specific antisense of asparate aminotransferase-P2

1996 ◽  
Vol 5 (2) ◽  
pp. 105-113 ◽  
Author(s):  
Vadim L. Mett ◽  
Ellen Podivinsky ◽  
Andrew M. Tennant ◽  
Leesa P. Lochhead ◽  
William T. Jones ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Plants ◽  
Tissue Specific

scholarly journals Combinatorial Control of Suicide Gene Expression by Tissue-specific Promoter and microRNA Regulation for Cancer Therapy

2009 ◽  
Vol 17 (12) ◽  
pp. 2058-2066 ◽  
Author(s):  
Chunxiao Wu ◽  
Jiakai Lin ◽  
Michelle Hong ◽  
Yukti Choudhury ◽  
Poonam Balani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cancer Therapy ◽  
Suicide Gene ◽  
Tissue Specific ◽  
Microrna Regulation ◽  
Tissue Specific Promoter ◽  
Combinatorial Control

Creation of Bt Rice Expressing a Fusion Protein of Cry1Ac and Cry1I-Like Using a Green Tissue-Specific Promoter

2014 ◽  
Vol 107 (4) ◽  
pp. 1674-1679 ◽  
Author(s):  
Yong-Yi Yang ◽  
Feng Mei ◽  
Wei Zhang ◽  
Zhicheng Shen ◽  
Jun Fang
Keyword(s):  
Fusion Protein ◽  
Tissue Specific ◽  
Bt Rice ◽  
Green Tissue ◽  
Tissue Specific Promoter
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