scholarly journals In vitro micrografting of lucumo (Pouteria lucuma), Sapotaceae

2017 ◽  
Keyword(s):  
In Vitro Micrografting

In vitro Micrografting of Mature Pistachio (Pistacia vera var. Siirt)

2004 ◽  
Vol 77 (2) ◽  
pp. 215-219 ◽  
Author(s):  
A. Onay ◽  
V. Pirinç ◽  
H. Yıldırım ◽  
D. Basaran
Keyword(s):  
Pistacia Vera ◽  
In Vitro Micrografting

Viroid Elimination by Thermotherapy, Cold Therapy, Tissue Culture, In Vitro Micrografting, or Cryotherapy

2017 ◽  
pp. 425-435 ◽  
Author(s):  
Marina Barba ◽  
Munetaka Hosakawa ◽  
Qiao-Chun Wang ◽  
Anna Taglienti ◽  
Zhibo Zhang
Keyword(s):  
Tissue Culture ◽  
Culture In Vitro ◽  
Cold Therapy ◽  
In Vitro Micrografting

Rajeunissement de clones matures d'Hevea brasiliensis (Müll. Arg.) par microgreffage in vitro

1994 ◽  
Vol 74 (3) ◽  
pp. 623-630 ◽  
Author(s):  
Y. Perrin ◽  
L. Lardet ◽  
F. Enjalric ◽  
M. P. Carron
Keyword(s):  
Key Words ◽  
Hevea Brasiliensis ◽  
Culture Medium ◽  
Shoot Elongation ◽  
Nodal Explants ◽  
Axillary Shoots ◽  
Juvenile Material ◽  
In Vitro Micrografting ◽  
Positive Effect

In vitro micrografting of apices of two mature genotypes of Hevea brasiliensis (Müll. Arg.), IRCA 18 and PB 235, on 3-wk-old seedlings grown in vitro permitted successfull in vitro micro-cutting of these two genotypes. Microcutting of micrografted material was impossible from nodal explants or shoot tips of scions developed in vitro on rootstocks. Such explants were incapable of caulogenesis activity after their isolation. This problem was resolved using mixed explants, each consisting of a part of the rootstock in contact with the culture medium, and of the clonal scion from which axillary shoots are developed regularly along the subcultures. Budding and shoot elongation abilities along the subcultures have been compared between mixed explants from micrografts, nodal explants from juvenile material and nodal explants from non-micrografted mature genotypes. The results show a very positive effect of micrografting on the in vitro caulogenesis ability in both genotypes. Moreover, shoots produced by mixed explants from micrografts exhibit the same rooting ability as shoots produced by explants collected on juvenile material. Key words: Rejuvenation, Hevea brasiliensis, in vitro micrografting, micropropagation, in vitro microcutting

IN VITRO MICROGRAFTING PROTOCOL IN LYCOPERSICON ESCULENTUM

2009 ◽  
pp. 365-370
Author(s):  
M.M. do Rêgo ◽  
E.R. do Rêgo ◽  
O. de Lima Coutinho
Keyword(s):  
Lycopersicon Esculentum ◽  
In Vitro Micrografting

scholarly journals Teknik sambung mikro in vitro kina Cinchona succirubra dengan C. ledgeriana In vitro micrografting technique of Chincona succirubra and C. ledgeriana

2016 ◽  
Vol 74 (2) ◽  
Author(s):  
Nurita TORUAN-MATHIUS ◽  
. LUKMAN ◽  
. AGUS-PURWITO
Keyword(s):  
Tissue Culture ◽  
Clonal Propagation ◽  
Ms Medium ◽  
Randomized Design ◽  
Top Soil ◽  
Completely Randomized Design ◽  
Reduced Light ◽  
In Vitro Micrografting ◽  
Cinchona Ledgeriana

Summary In vitro micrografting is a technique for grafting scions to rootstocks of plantlets from tissue culture. In vitro micrografting of Cinchona plant has never been carried out. The objective of this research was to obtain the best method of in vitro micrografting, medium for micrografted plantlets, and acclimatization  for Cinchona plantlets from  micrografting. The research consisted of (i) optimization of micrografting method, (ii) optimization of medium for growing plantlets, and (iii) acclimatization of micrografted plantlet. Plantlets of four-month-old of  C. ledgeriana  QRC clone were used as  scions, while of C. succirubra as  rootstocks. Each of experiments was arranged according to Completely Randomized Design, consisted of  combination of scion and rootstock and type of micro-grafting with 10 replicates. Parameters measured were  the percentage of survived plantlet, leaf number, and callus productions on union area, and percentage of survived  plantlet. The results show that V type of micrografting was the best for Cinchona micrografting. MS medium with the addition of 3 mg/L IBA was the best medium for growing of micrografted plantlet. Husk charcoal mixed with top soil (1 : 1) was the best medium for acclimatization.  Acclimatization  consisted  of two steps: preaclimatization in a culture room with 12- hour photoperiod at temperature 25 – 27oC  for two weeks,  followed by aclimatization in a plastic house with  70% reduced light intensity for one month. Using this method, 90% of the seedlings were survived. It is concluded that in vitro micrografting can be used as a technique for clonal propagation of Cinchona sp.Ringkasan  Teknik sambung mikro (mikrografting) in vitro adalah teknik penyambungan potongan batang atas pada batang bawah dalam kultur jaringan.  Pada tanaman kina teknik sambung mikro  in vitro belum pernah dilakukan. Tujuan penelitian ini adalah  menetapkan tipe sambung mikro, medium terbaik untuk planlet hasil sambung  mikro, dan perbanyakan tanaman kina dengan sambung mikro. Pelaksanaan percobaan meliputi (i) optimasi tipe sambung, (ii) optimasi  medium, dan (iii) aklimatisasi planlet hasil sambung mikro. Bahan tanaman yang digunakan sebagai batang atas adalah planlet Cinchona ledgeriana klon QRC, sedangkan sebagai batang bawah digunakan planlet  C. succirubra, berumur empat bulan. Masing- masing percobaan disusun dengan Rancangan Acak Lengkap terdiri dari dua taraf yaitu  kombinasi batang bawah dengan batang atas bentuk sambung tipe V dan L dilakukan  dengan 10 ulangan. Peubah yang diukur meliputi persentase planlet yang bertahan hidup,  jumlah daun,  berkalus atau tidak berkalus pada daerah pertautan, dan persentase planlet yang bertahan hidup. Hasil yang diperoleh menunjukkan bahwa tipe V merupakan cara sambung  mikro  yang terbaik. Medium MS dengan penambahan 3 mg/L IBA adalah medium terbaik untuk pertumbuhan dan perakaran planlet hasil sambung mikro.  Aklimatisasi planlet dilakukan dengan medium tumbuh arang sekam : top soil (1 : 1) yang disterilkan. Tahapan aklimatisasi adalah pre-aklimatisasi dalam ruang kultur  suhu 25 -     27 oCdengan pencahayaan 12 jam per hari dan diikuti dengan aklimatisasi di rumah plastik bernaungan 70% paranet. Dengan metode aklimatisasi ini  90% dari bibit mampu bertahan hidup. Kesimpulan dari penelitian ini menunjukkan bahwa teknik sambung mikro dapat digunakan untuk perbanyakan klonal   Cinchona sp..

Rajeunissement chez le Sequoia sempervirens: effets du microgreffage in vitro

1991 ◽  
Vol 69 (8) ◽  
pp. 1772-1779 ◽  
Author(s):  
H. Tranvan ◽  
F. Bardat ◽  
M. Jacques ◽  
Y. Arnaud
Keyword(s):  
Key Words ◽  
Sequoia Sempervirens ◽  
Physiological Characteristics ◽  
Leafy Shoots ◽  
In Vitro Micrografting

In vitro micrografting of Sequoia sempervirens was used as a rejuvenation method: apices were collected from in vitro cultured shoots issued from a 500-year-old tree; the rootstocks originated from a 1-year-old tree. Only a few grafted apices survived. Once producing leafy shoots, they exhibited some juvenile morphological and physiological characteristics. Some scions were isolated and rooted in a new medium. Although the restoration of the orthotropy is very difficult, a few of the rooted plantlets were orthotropic and this characteristic was maintained after acclimatization in greenhouses. Therefore, the in vitro micrografting of Sequoia sempervirens can be considered as a rejuvenation method that must be improved. Key words: Sequoia sempervirens, in vitro micrografting, rejuvenation, orthotropy.

In vitro micrografting of apical and axillary buds of cacao

2016 ◽  
Vol 92 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Yulien Miguelez-Sierra ◽  
Annia Hernández-Rodríguez ◽  
Yanelis Acebo-Guerrero ◽  
Marie Baucher ◽  
Mondher El Jaziri
Keyword(s):  
Axillary Buds ◽  
In Vitro Micrografting
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