Rajeunissement chez le Sequoia sempervirens: effets du microgreffage in vitro

1991 ◽  
Vol 69 (8) ◽  
pp. 1772-1779 ◽  
Author(s):  
H. Tranvan ◽  
F. Bardat ◽  
M. Jacques ◽  
Y. Arnaud
Keyword(s):  
Key Words ◽  
Sequoia Sempervirens ◽  
Physiological Characteristics ◽  
Leafy Shoots ◽  
In Vitro Micrografting

In vitro micrografting of Sequoia sempervirens was used as a rejuvenation method: apices were collected from in vitro cultured shoots issued from a 500-year-old tree; the rootstocks originated from a 1-year-old tree. Only a few grafted apices survived. Once producing leafy shoots, they exhibited some juvenile morphological and physiological characteristics. Some scions were isolated and rooted in a new medium. Although the restoration of the orthotropy is very difficult, a few of the rooted plantlets were orthotropic and this characteristic was maintained after acclimatization in greenhouses. Therefore, the in vitro micrografting of Sequoia sempervirens can be considered as a rejuvenation method that must be improved. Key words: Sequoia sempervirens, in vitro micrografting, rejuvenation, orthotropy.

Rajeunissement de clones matures d'Hevea brasiliensis (Müll. Arg.) par microgreffage in vitro

1994 ◽  
Vol 74 (3) ◽  
pp. 623-630 ◽  
Author(s):  
Y. Perrin ◽  
L. Lardet ◽  
F. Enjalric ◽  
M. P. Carron
Keyword(s):  
Key Words ◽  
Hevea Brasiliensis ◽  
Culture Medium ◽  
Shoot Elongation ◽  
Nodal Explants ◽  
Axillary Shoots ◽  
Juvenile Material ◽  
In Vitro Micrografting ◽  
Positive Effect

In vitro micrografting of apices of two mature genotypes of Hevea brasiliensis (Müll. Arg.), IRCA 18 and PB 235, on 3-wk-old seedlings grown in vitro permitted successfull in vitro micro-cutting of these two genotypes. Microcutting of micrografted material was impossible from nodal explants or shoot tips of scions developed in vitro on rootstocks. Such explants were incapable of caulogenesis activity after their isolation. This problem was resolved using mixed explants, each consisting of a part of the rootstock in contact with the culture medium, and of the clonal scion from which axillary shoots are developed regularly along the subcultures. Budding and shoot elongation abilities along the subcultures have been compared between mixed explants from micrografts, nodal explants from juvenile material and nodal explants from non-micrografted mature genotypes. The results show a very positive effect of micrografting on the in vitro caulogenesis ability in both genotypes. Moreover, shoots produced by mixed explants from micrografts exhibit the same rooting ability as shoots produced by explants collected on juvenile material. Key words: Rejuvenation, Hevea brasiliensis, in vitro micrografting, micropropagation, in vitro microcutting

scholarly journals KARAKTERISTIK FISIOLOGI ISOLAT Pleurotus spp.

2020 ◽  
Vol 15 (1) ◽  
pp. 46
Author(s):  
ACHMAD ACHMAD ◽  
ELIS NINA HERLIYANA ◽  
OSICA ASNO FERINA YURTI ◽  
ANANG PRANOTO HIDAYAT
Keyword(s):  
Key Words ◽  
Gallic Acid ◽  
Agar Medium ◽  
Physiological Characteristics ◽  
Medium Temperature ◽  
Physiological Character ◽  
Diameter Range ◽  
Temperature Levels

<p>ABSTRAK</p><p>Studi in vitro tentang karakteristik fungi isolat Pleurotus spp. telahdilaksanakan di Bogor dari bulan Juli sampai Agustus 2004. Penelitianmenggunakan rancangan faktorial dalam rancangan acak lengkap danbertujuan untuk mempelajari pengaruh media, temperatur inkubasi dan pHmedia terhadap 6 isolat Pleurotus sp. Karakter lain yang juga dipelajariadalah kemampuan untuk mengoksidasi asam tanat dan asam galat dalammedia agar. Hasil penelitian menunjukkan bahwa Pleurotus isolat sp.6dan sp.8 tumbuh baik pada media MPA, isolat Pleurotus sp.1, 3 dan 4pada media MEA dan isolate sp.2 pada media PDA. Kecuali isolat sp.8,isolat lainnya tidak dapat tumbuh pada temperatur 10 dan 35 o C. Pertum-buhan isolat sp.8 terbaik dibandingkan isolat lainnya pada semuatemperatur. Diameter koloni isolat lainnya hanya mencapai 0,2 – 2,33 cm.Pertumbuhan isolat sp.8 juga terbaik pada semua pH media diikuti isolatsp.6 kemudian isolat sp.4. Semua isolat menunjukkan reaksi oksidasipositif pada agar asam tanat dan asam galat yang ditunjukkan oleh warnacoklat pada media yang melingkari koloni.</p><p>Kata kunci : Pleurotus spp, media, temperature, pH, oksidasi, asam tanat,asam galat</p><p>ABSTRACT</p><p>Physiological Characteristics of Pleurotus spp. IsolatesPhysiological characteristics of some Pleurotus sp. isolates werestudied in vitro, from July until August 2004 in Bogor. Experiments tostudy the effect of kind of media, temperature of incubation room, and pHof medium on six isolates of Pleurotus sp. were arranged in factorialrandomized complete design and replicated three times with colony in apetri dish as experimental units. Another physiological character studiedwas the ability to oxidize tannic and gallic acids in agar medium. Resultsshowed that isolates Pleurotus sp.6 and -8 grew better in MPA medium,Pleurotus sp.1, -3, and -4 in MEA, and Pleurotus sp.2 in PDA. ExceptPleurotus sp.8, other isolates could not grow in incubation roomtemperature of 10 and 35 o  C. The growth of Pleurotus sp.8 was the bestamong the isolates in all temperature levels. Other isolates grew poorly in20 and 29o C with diameter range was 0.2 – 2.33 cm. The growth ofPleurotus sp.8 was also the best in all pH medium levels, followed byPleurotus sp.6, and then Pleurotus sp.4. All isolates showed positiveoxidative reaction on tannic and gallic acid agar indicated by brown colorof the medium around the colony.</p><p>Key words: Pleurotus spp., medium, temperature, pH, oxidation, tannicacid, gallic acid</p>

scholarly journals GLUCOSE CONSUMPTION AND LACTIC ACID PRODUCTION BY ERYTHROCYTES FROM DOUBLE-MUSCLED CATTLE

1982 ◽  
Vol 62 (3) ◽  
pp. 751-757 ◽  
Author(s):  
J. A. BASARAB ◽  
R. T. BERG ◽  
J. R. THOMPSON
Keyword(s):  
Lactic Acid ◽  
Key Words ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Age Groups ◽  
Phenotypic Expression ◽  
Glucose Consumption ◽  
Molar Ratio ◽  
Breed Group

The in vitro glucose consumption and lactic acid production by erythrocytes from 20 cattle of a Beef Synthetic (SY) breed group and 25 cattle of a double-muscled (DM) breed group were determined. There were three age groups and two sexes within each breed group. Animals within the DM breed group were categorized as either phenotypically normal- to moderate-muscled (DM carriers) or extreme-muscled (extreme DM) based on the phenotypic expression of the double-muscling trait and on their breeding history. Both DM phenotypes had higher (P < 0.01) erythrocyte glucose consumption and lactic acid production than normal-muscled, noncarrier cattle of the SY breed group. Extreme DM cattle were not different (P > 0.05) in either their erythrocyte glucose consumption or lactic acid production compared with DM carriers. No difference (P > 0.05) due to breed or phenotype was observed in the molar ratio of lactic acid produced to glucose consumed by erythrocytes. These results suggest that carriers of the Double Muscled Syndrome, regardless of phenotypic expression of the double-muscling trait, have a higher rate of erythrocyte glycolysis than normal cattle. Key words: Cattle, double muscled, erythrocyte, glucose, lactic acid

Results of an experimental study of hydrogel drainage containing ranibizumab in antiglaucoma operations

2021 ◽  
pp. 63-67
Author(s):  
I.I. Khusnitdinov ◽  
Keyword(s):  
Experimental Study ◽  
Hyaluronic Acid ◽  
Key Words ◽  
Experimental Research ◽  
Experimental Studies ◽  
Glaucoma Surgery ◽  
Control Group ◽  
Morphological Studies

Purpose. Еxperimental substantiation of the effectiveness of biocompatible biodegradable hydrogels based on hyaluronic acid and chitosan succinate as a carrier of ranibizumab in antiglaucoma operations. Material and methods. Hydrogel drainage (HD) was obtained immediately before surgery. A solution of ranibizumab (0.23 ml) was mixed with a solution of hyaluronic acid dialdehyde (0.5 ml), then a solution of chitosan succinate (0.5 ml) was added. Experimental studies were performed in 12 (12 eyes) healthy rabbits. The first group consisted of 6 eyes – 0.187 ml of ranibizumab per 1 ml of gel. In the control group, HD was used intraoperatively without the addition of ranibizumab (6 eyes). Morphological studies were performed on 7th, 21st, and 42nd days. Results. In experimental studies in vitro and in vivo, it was proved that ranibizumab, administered as a part of 0.1 ml of hydrogel drainage in the antiglaucoma surgery area is released within 3 weeks and suppresses vascularization, scarring of the operating area, and preserves the intrascleral cavity. The optimal concentration of ranibizumab was selected-0.02 ml in 0.1 ml of gel. Conclusion. The safety and effectiveness of the use of hydrogel drainage with ranibizumab based on hyaluronic acid dialdehyde and chitosan succinate in anti-glaucoma operations has been proven. Key words: experimental research, hydrogel drainage, ranibizumab, glaucoma surgery.

scholarly journals Effects of Growth Regulators on In vitro Propagation and Tuberization of Four Dioscorea Species

1970 ◽  
Vol 18 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Md. Tariqul Islam ◽  
E. R. Joachim Keller ◽  
D. Philibert Dembele
Keyword(s):  
Key Words ◽  
Growth Regulators ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
In Vitro Production ◽  
Dioscorea Species ◽  
Vitro Production ◽  
Species Being ◽  
Nodal Culture

Nodal explants of 12 accessions from four species of yam (Dioscorea spp.) were cultured for six weeks on MS to evaluate the influence of IAA, Kn, NAA and BAP on the production of leaves and microtubers. Four Dioscorea polystachya Turcz., three each of D. bulbifera L. and D. sansibarensis Pax. and two D. japonica Thunb. accessions were used. Five and 10 mg/l of Kn along with IAA and sucrose, and 0.2 and 0.5 mg/l of NAA, sucrose and with or without BAP were used in four treatments. The accessions Yam 23 and Yam 25 of D. sansibarensis failed to initiate any leaf under four treatments. The remaining accessions produced 0.11 to 1.76 leaves per explant. The medium containing IAA with higher concentration of Kn (10 mg/l) and 3% sucrose was found to be best for in vitro production of leaf (0.71/explant) and the most productive species was D. japonica (1.36), followed by D. polystachya (1.19/explant). At the same culture period, Yam 16 of D. bulbifera failed to initiate any microtuber at IAA with Kn, and NAA with or without BAP. The remaining accessions produced 0.09 to 1.15 microtubers per explant. Lower concentration of Kn (5 mg/l) with IAA and sucrose was favourable for producing microtubers (0.61/explant on an average), the best species being D. sansibarensis (1.27) followed by D. japonica (0.59/ explant). Finally, the presence of BAP adversely affected the production of microtuber among Dioscorea species. Key words: Dioscorea spp., Microtuber, Nodal culture, Propagation D.O.I. 10.3329/ptcb.v18i1.3260 Plant Tissue Cult. & Biotech. 18(1): 25-35, 2008 (June)

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

A new Botryoascus from the air of a poultry farm

1990 ◽  
Vol 68 (8) ◽  
pp. 1738-1740 ◽  
Author(s):  
Angel T. Martínez ◽  
Aldo E. González ◽  
Lourdes Abarca ◽  
Javier Cabanes
Keyword(s):  
Key Words ◽  
Physiological Characteristics ◽  
Poultry Farm

A new endomycetaceous fungus, Botryoascus cladosporoides, is described. This species differs from the previously described Botryoascus synnaedendrus in conidial structures and physiological characteristics and occurs in a very different habitat. Key words: yeasts, endomycetes, taxonomy, aeromycology.

An attempt at sperm-mediated gene transfer in mice and chickens

1991 ◽  
Vol 71 (2) ◽  
pp. 287-291 ◽  
Author(s):  
J. S. Gavora ◽  
B. Benkel ◽  
H. Sasada ◽  
W. J. Cantwell ◽  
P. Fiser ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Gene Transfer ◽  
Transgenic Mice ◽  
Key Words ◽  
Plasmid Dna ◽  
Dna Transfer ◽  
Laboratory Mice ◽  
Vitro Fertilization ◽  
Bacterial Plasmid

Experiments were carried out to transform laboratory mice and domestic chickens by use of sperm incubated with bacterial plasmid DNA. Following demonstration of "uptake" of such DNA by sperm of both species, attempts were made to replicate a previously published procedure (Lavitrano et al. 1989, Cell 57: 717–723) for producing transgenic mice through in vitro fertilization (IVF). Also, female mice and hens were inseminated (AI) with sperm which had been incubated in a DNA solution. Such incubation did not influence the fertility or hatchability of the hens' eggs. However, no transformed progeny were detected among 45 mice produced by IVF or among 69 mice and 470 chickens produced by AI. Key words: Sperm-mediated DNA transfer, mice, chickens

Follicular steroidogenesis in vitro in cyclic and PMSG/hCG-treated gilts

1991 ◽  
Vol 71 (4) ◽  
pp. 1257-1260
Author(s):  
T. Wiesak ◽  
R. T. Hardin ◽  
G. R. Foxcroft ◽  
M. G. Hunter
Keyword(s):  
Key Words ◽  
Follicular Development ◽  
Steroid Production ◽  
Functional Differences ◽  
Key Words Pig

Basal steroid production in vitro by comparable follicles from cyclic and PMSG/hCG-treated gilts was similar. Follicular responses to LH in vitro varied, however, confirming earlier evidence for functional differences in the pattern of follicular development in immature gilts in response to exogenous gonadotropins compared with naturally cyclic gilts. Key words: Pig, gonadotrophin, follicle, steroidogenesis, steroids

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