scholarly journals PROTECTIVE EFFECT OF METHANOL EXTRACT OF RUSSELIA EQUISETIFORMIS AGAINST PARACETAMOL-INDUCED HEPATOTOXICITY IN WISTAR RATS

2021 ◽  
Author(s):  
Wakeel Olayemi Kamoru ◽  
Ayankunle Akeem Ademola ◽  
Oluogun Waheed Akanni ◽  
Kolawole Oyetunji Timothy ◽  
Adeyeba Oluwaseyi Adegboyega
Keyword(s):  
Protective Effect ◽  
Peer Review ◽  
Total Protein ◽  
Wistar Rats ◽  
Methanol Extract ◽  
Serum Levels ◽  
Research Centre ◽  
Dependent Manner ◽  
Anti Oxidant ◽  
Anti Inflammatory

Objectives: Liver diseases are among the health challenges facing many people and health care providers worldwide. In their search for solution to these problems, researchers are increasingly advocating the use of herbal preparations with proven efficacy in protecting against hepatic disorders. They also investigate medicinal plants with the aim of developing new drugs.  Russelia equisetiformis is a plant which contains phytoconstituents that were reported to have biological activities, such as anti-inflammatory, antidiabetic, and membrane-stabilizing properties. In this study, the effect of methanol extract of R. equisetiformis (MEREQ) on paracetamol-induced hepatotoxicity was investigated in rats. Method: Rats were pretreated orally with graded doses (100 – 400 mg/kg b.w) of MEREQ for 7 days. On the 8th day, hepatotoxicity was induced in the pretreated rats with a single intraperitoneal injection of paracetamol (2 g/kg b.w). Rats were sacrificed on the 15th day; blood samples were taken for biochemical analysis, and the liver was excised for histopathological study. Biochemical parameters analyzed are alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein, albumin, and bilirubin. Results: Administration of paracetamol in the rats resulted in significant increase (p<0.05) in the serum levels of AST, ALT, ALP, and bilirubin compared with the control. Treatment with MEREQ significantly reduced (p<0.05) the levels of these parameters in a dose-dependent manner, compared with the untreated rats. No significant changes were observed in the serum levels total protein and albumin. Histopathological examination showed that administration of paracetamol caused distortions in the architecture of the liver, but the degree of degeneration of hepatocytes was reduced in the MEREQ-treated rats. Conclusion: From the results obtained in this study, it is concluded that methanol extract of R. equisetiformis has protective effect on paracetamol-induced hepatic injury.                                 Peer Review History: Received 8 November  2020; Revised 14 Decembe; Accepted 3 January, Available online 15 January 2021 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file:                           Comments of reviewer(s):         Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 8.0/10 Reviewer(s) detail: Dr. Gehan Fawzy Abdel Raoof Kandeel, Pharmacognosy Department, National Research Centre, Dokki, 12622,  Giza, Egypt, [email protected]  Prof. Dr. Ali Gamal Ahmed Al-kaf, Sana'a university, Yemen, [email protected] Similar Articles: ANTI-INFLAMMATORY AND ANTI-OXIDANT ACTIVITIES OF METHANOL EXTRACT OF BAPHIA NITIDA ANTI-HYPERGLYCEMIC AND ANTI-OXIDANT ACTIVITIES OF METHANOL EXTRACT OF GONGRONEMA LATIFOLIUM EFFECTS OF RAW AND COOKED AQUEOUS AND METHANOL EXTRACTS OF PHASEOLUS VULGARIS (KIDNEY BEANS) ON RENAL FUNCTION IN ALBINO WISTAR RATS

BIOCHEMICAL AND HISTOPATHOLOGICAL EFFECTS OF HYDROETHANOLIC EXTRACT OF IRVINGIA WOMBOLU KERNELS ON RAT LIVER AND KIDNEY

2021 ◽  
Author(s):  
Oyetunji Timothy Kolawole ◽  
Akeem Ademola Ayankunle ◽  
Olayemi Kamoru Wakeel ◽  
Anthony Tope Olofinnade ◽  
Olatunde Samson Olaniyi ◽  
...  
Keyword(s):  
Peer Review ◽  
Total Protein ◽  
Wistar Rats ◽  
Myocardial Damage ◽  
Serum Levels ◽  
Stem Bark ◽  
The Body ◽  
African Countries ◽  
Medicinal And Aromatic Plants ◽  
North East

Objective: Many plants are consumed as food by humans for growth and survival, but a large number of these plants have not been tested for toxicity potential. Repeated consumption of such plants could lead to accumulation of toxic chemicals in the body and cause health-related problems. Irvingia wombolu kernel is widely consumed by many ethnic groups in Nigeria and some other African countries. The toxicity potentials of Irvingia wombolu kernel extract (IWKE) on the kidney and liver of rats was evaluated in this study. Methods: Three groups of Wistar rats were fed orally with IWKE (50, 100, and 200 mg/kg b.w) daily for 28 days. The fourth group which is the control was treated with distilled water (10 ml/kg b.w) for the same period.  The rats were sacrificed on the 29th day, and blood samples, kidney and liver were harvested for analyses. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein, albumin, bilirubin, creatinine, and urea were determined. Kidney and liver sections were examined for histopathological changes. Data were subjected to Student’s t-test for statistical analysis. Results: Serum levels of creatinine and urea were not significantly altered in the IWKE-treated rats compared with the control. At 200 mg/kg b.w, the extract significantly increased (p<0.05) serum levels of ALT, AST, total bilirubin, total protein, and albumin compared with the control. Significant distortions were observed in the liver sections of rats treated with 200 mg/kg b.w IWKE compared with control, but the structure of the kidney section of IWKE-treated rats was not significantly different from the control. Conclusion: The results showed that repeated ingestion of Irvingia wombolu kernel at a dose of 200 mg/kg b.w for 28 days induced liver damage, but does not significantly affect renal function.                       Peer Review History: Received: 9 September 2021; Revised: 12 October; Accepted: 27 October, Available online: 15 November 2021 Academic Editor:  Dr. Ali Abdullah Al-yahawi, Al-Razi university, Department of Pharmacy, Yemen, [email protected] UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.  Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.5/10 Reviewers: Dr. Sangeetha Arullappan, Universiti Tunku Abdul Rahman, Malaysia, [email protected] Ahmad Najib, Universitas Muslim Indonesia, Makassar, Indonesia, [email protected] Taha A.I. El Bassossy, Medicinal and Aromatic Plants Department, Desert Research Center, Cairo, Egypt, [email protected] Dr. Nazim Hussain, North East Frontier Technical University, Arunachal pradesh, India, [email protected] Similar Articles: MYOCARDIAL POTENCY OF AQUEOUS EXTRACT OF HARUNGANA MADAGASCARIENSIS STEM BARK AGAINST ISOPROTERENOL-INDUCED MYOCARDIAL DAMAGE IN RATS PROTECTIVE EFFECT OF METHANOL EXTRACT OF RUSSELIA EQUISETIFORMIS AGAINST PARACETAMOL-INDUCED HEPATOTOXICITY IN WISTAR RATS

scholarly journals EFFECTS OF RAW AND COOKED AQUEOUS AND METHANOL EXTRACTS OF PHASEOLUS VULGARIS (KIDNEY BEANS) ON RENAL FUNCTION IN ALBINO WISTAR RATS

2020 ◽  
Author(s):  
Idoko A ◽  
Philip OC ◽  
Nwali ON ◽  
Ugwudike PO ◽  
Blessing NO ◽  
...  
Keyword(s):  
Renal Function ◽  
Phaseolus Vulgaris ◽  
Peer Review ◽  
Wistar Rats ◽  
Methanol Extract ◽  
Control Group ◽  
Aqueous Extracts ◽  
Methanol Extracts ◽  
Anti Oxidant ◽  
E Mail

Objective: Phaseolus vulgaris, like other beans, is endowed with rich nutritional contents. This study evaluated the effects of raw and cooked aqueous and methanol extracts of P. vulgaris on renal function in albino Wistar rats. Methods: Oral acute toxicity (LD50) study of both extracts was conducted in two phases. In the main design, a total 36 Wistar albino rats were used and divided into nine groups of four rats and oral administration lasted for 7 days. Group 1 served as control and 2 – 9 treated groups. Groups 2 and 3; 4 and 5 were administered aqueous extracts while groups 6 and 7; 8 and 9 were administered methanol extracts of 350mg/kg and 550mg/kg body weight raw and cooked P. vulgaris respectively. Results: Results of LD50 of all extracts were greater than 5000mg/kg. Results showed a significant (P<0.05) increase in concentrations of urea and chloride across test groups administered aqueous extracts, than methanol extracts; a significant (P<0.05) increase in serum creatinine in test groups administered methanol extracts; a significant (P<0.05) increase of serum total protein of test groups compared to control; no significant (P<0.05) difference in the concentration of potassium in test groups administered compared to control group. Conclusion: It may be concluded that P. vulgaris portrays potentials capable of improving renal function and its consumption may contribute to the wellness of a person due to its rich nutrients, and based on the duration of this work and standard scale of toxicity; the extracts are practically non- toxic since the LD50 was greater than 5000mg/kg.   Peer Review History: Received 7 June 2020; Revised 25 June; Accepted 3 July, Available online 15 July 2020   UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 8.0/10 Reviewer(s) detail: Name: Dr. Ali Gamal Ahmed Al-kaf Affiliation: Sana'a university, Yemen E-mail: [email protected]   Name: Ahmad Najib Affiliation: Universitas Muslim Indonesia, Makassar, Indonesia E-mail: [email protected]   Comments of reviewer(s): Similar Articles: ANTI-INFLAMMATORY AND ANTI-OXIDANT ACTIVITIES OF METHANOL EXTRACT OF BAPHIA NITIDA EVALUATION OF METHANOLIC EXTRACT OF EUPHORBIA NERIIFOLIA STEM BARK ON BLOOD SUGAR LEVELS, SERUM AND TISSUE LIPIDS IN A PRECLINICAL MODEL ANTI-HYPERGLYCEMIC AND ANTI-OXIDANT ACTIVITIES OF METHANOL EXTRACT OF GONGRONEMA LATIFOLIUM

scholarly journals Analgesic and Anti-Inflammatory Activities of the Methanol Extract fromPogostemon cablin

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Tsung-Chun Lu ◽  
Jung-Chun Liao ◽  
Tai-Hung Huang ◽  
Ying-Chih Lin ◽  
Chia-Yu Liu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Methanol Extract ◽  
Second Phase ◽  
Dependent Manner ◽  
Paw Edema ◽  
Pogostemon Cablin ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Factor Α

Pogostemon cablin(PC) is a herbal medicine traditionally applied to treat not only common cold, nausea and diarrhea but also headache and fever. The aim of this study was to investigate the analgesic and anti-inflammatory properties of standardized PC methanol extract (PCMeOH)in vivo. Investigations were performed in mice with two analgesic models. One was acetic acid-induced writhing response and the other formalin-induced paw licking. The anti-inflammatory effect was tested by λ-carrageenan (Carr)-induced mice paw edema. These analgesic experimental results indicated that PCMeOH (1.0 g/kg) decreased the acetic acid-induced writhing responses and PCMeOH (0.5 and 1.0 g/kg) decreased the licking time in the second phase of the formalin test. Moreover, Carr-induced paw edema inflammation was significantly reduced in a dose-dependent manner when PCMeOH (0.5 and 1.0 g/kg) was administered 3 and 4 h after the Carr injection. Mechanistic studies showed that PCMeOH decreased the levels of malondialdehyde in the edema paw by increasing the activities of anti-oxidant enzymes, such as superoxide dismutase, glutathione peroxidase and glutathione reductase, in the liver and decreasing the cyclooxygenase 2 and tumor necrosis factor-α activities in the edema paw. This study has demonstrated the analgesic and anti-inflammatory effects of PCMeOH, thus verifying its popular use in traditional medicine.

scholarly journals Anti-oxidant, Anti-inflammatory and Anti-cancer Effect of Methanol Extract of Pogostemon cablin

2015 ◽  
Vol 25 (1) ◽  
pp. 44-52 ◽  
Author(s):  
Seung Geun Yun ◽  
Soojung Jin ◽  
Hyun Young Jeong ◽  
Hee Jung Yun ◽  
Mi young Do ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Pogostemon Cablin ◽  
Anti Cancer ◽  
Anti Oxidant ◽  
Anti Inflammatory

scholarly journals EFFECT OF SUB-CHRONIC ORAL ADMINISTRATION OF HYDROMETHANOLIC STEM EXTRACT OF COSTUS AFER KER GAWL. (COSTACEAE) ON LIVER AND KIDNEY FUNCTIONS IN WISTAR RATS

2020 ◽  
Vol 4 (2) ◽  
pp. 317-324
Author(s):  
A. A. Jimoh ◽  
B. B. Maiha ◽  
B. A. Chindo ◽  
J. I. Ejiofor
Keyword(s):  
Total Protein ◽  
Wistar Rats ◽  
Serum Levels ◽  
Hepatic Necrosis ◽  
Chloride Ions ◽  
The Body ◽  
Albumin Concentration ◽  
Liver And Kidney ◽  
Stem Extract ◽  
Costus Afer

The liver and the kidneys are two very important organs in the body and they are responsible for the metabolism and excretion of drugs respectively amongst several other functions. Severe adverse effects on these organs can lead to organ dysfunction or failure and a consequential effect on wellbeing and can even be life-threatening. This study investigated the effects of hydromethanolic stem extract of Costus afer Ker Gawl. (Costaceae) on liver and kidney function indices and the histopathology of the organs in Wistar rats. Serum liver enzymes which include: alanine amino transferase (ALT), aspartate amino transferase (AST) and alkaline phosphatase (ALP), total protein and albumin as well as serum urea, creatinine, sodium ions, potassium ions, chloride ions, bicarbonate ions were evaluated in biochemical studies. Sections of the liver and kidneys appropriately treated were examined microscopically for pathological lesions.There were decreased serum levels of ALT and ALP, but serum levels of AST increased at 500 and 1000 mg/kg doses. Serum levels of total protein (TP) and albumin concentration as well as urea and creatinine serum levels were not significantly (p>0.05) affected. However, histological examination of the liver and kidneys revealed slight to moderate hepatic necrosis and slight tubular necrosis respectively especially at 500 and 100 mg/kg doses of the extract. The results showed that the extract may be harmful to the liver and to a lesser extent the kidneys on prolonged administration and therefore it should be used with caution in such instances.

Abstract 16995: Anti-Inflammatory and Immuno-Modulatory Properties of Neonatal MSC

Circulation ◽  
2020 ◽  
Vol 142 (Suppl_3) ◽  
Author(s):  
Sudhish Sharma ◽  
Rachana Mishra ◽  
Progyaparamita Saha ◽  
Muthukumar Gunasekaran ◽  
Aakash Shah ◽  
...  
Keyword(s):  
Cell Therapy ◽  
Serum Levels ◽  
Cell Types ◽  
Treg Cells ◽  
Dependent Manner ◽  
Iri Model ◽  
Ifn Gamma ◽  
T Lymphocyte Proliferation ◽  
Adult Mesenchymal Stem Cells ◽  
Anti Inflammatory

Background: Anti-inflammatory and immunomodulatory properties are prerequisites for the success of cell therapy as shown by adult Mesenchymal Stem cells (MSC) by reducing both B- and T-lymphocyte proliferation, in a paracrine dependent manner. Proteomics of neonatal MSCs (nMSCs) has identified the superior quality of secretome containing a significantly higher number of anti-inflammatory (68 vs 21 in aMSCs) molecules. Hypothesis: nMSCs have potent anti-inflammatory and immunomodulatory characteristics as compared to other cell types currently being used as cell therapy. Methods: nMSC were tested and compared to other cell types for: a) Hypo-immunogenicity (expression of MHCII in the presence of IFN-gamma), b) immunogenicity (reduce proliferation of human CD4+ T-cells in a co-culture mixed lymphocyte reaction (MLR), c) anti-inflammatory properties (reduce IL-8 secretion of macrophages in the presence of Nigericin, d) systematic immune response (using rat myocardial infarction (MI) model (IL10, TNF-a and IL12)) and e) Immunomodulation (CD4+ cells to T reg CD4 + /CD25 + /FoxP3 + ). Results: Expression of MHCII on nMSCs, after 72 hours of exposure to IFN-gamma and proliferation of CD4 + cells in MLR was significantly (40±3.8% and 55±4.3%, n=5) reduced as compared to aMSC or CDC. IL8 was measured in the supernatants of nMSC derived secretome reduced the secretion of IL8 from activated macrophages in a dose dependent manner (150 ug protein/ml). Intravenous injection of nMSCs in a rat MI model significantly reduced serum levels of of TNF-α and IL-12 while upregulated IL-10 and improving myocardial function. While BM-MSCs and aMSCs failed to significantly modulate immune system, nMSC significantly increased the number of Treg cells as compared to CDC. Similar results were observed with Pig IRI model. Conclusions: Together, in a head-to-head comparison, nMSCs outperformed all other competitive cell types currently under investigation for their tissue repairing role, including CDCs and BM-MSCs to achieve the highest clinical efficacy.

Evaluation of Anti-oxidant and Anti-pyretic activity of fruits of Garcinia pedunculata

2021 ◽  
pp. 2635-2638
Author(s):  
Mrinmoy Basak ◽  
Moksood Ahmed Laskar ◽  
Priyanka Goswami
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Traditional Use ◽  
Synthetic Drugs ◽  
Long Time ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Most of the synthetic drugs used at present as analgesic and anti-inflammatory agents cause many side effects and toxic effects. Many medicines of plant origin with analgesic and anti-inflammatory activity have been used since long time without adverse effects. The purpose of the study was to explore the anti-pyretic and anti-oxidant activity of fruits of Garcinia pedunculata. The fruit of G. pedunculata was collected from Shillong, Meghalaya and extraction is done by using methanol as a solvent. The methanol extract shows the presence of carbohydrate, glycoside, alkaloids, phenol and flavonoids. Acute anti-pyretic activity for the extract was investigated in pyrexia rat. Temperature level was determined after 1hours, 4 hours, 8 hours and 12 hours after giving the extract dose of 100mg/kg and 200mg/kg body weight, and it was found to produce a significant reduce in fever. DPPH radical scavenging activity (In-vitro anti-oxidant activity of G. pedunculata) of methanolic extract shows 38.31 as IC50 (µg/ml) whereas Ascorbic acid as standard in the same concentration shows 34.81 IC50 (µg/ml). The obtained result justified the traditional use of G. pedunculata as anti-pyretic and antioxidant purpose.

scholarly journals Anti-Inflammatory Effects of a Methanol Extract from the Marine SpongeGeodia cydoniumon the Human Breast Cancer MCF-7 Cell Line

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Susan Costantini ◽  
Giovanna Romano ◽  
Fabiola Rusolo ◽  
Francesca Capone ◽  
Eliana Guerriero ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Methanol Extract ◽  
Marine Sponges ◽  
Biologically Active ◽  
Dependent Manner ◽  
Human Breast ◽  
Normal Human Breast ◽  
Anti Inflammatory ◽  
Mcf 7

Many research groups are working to find new possible anti-inflammatory molecules, and marine sponges represent a rich source of biologically active compounds with pharmacological applications. In the present study, we tested different concentrations of the methanol extract from the marine sponge,Geodia cydonium, on normal human breast epithelial cells (MCF-10A) and human breast cancer cells (MCF-7). Our results show that this extract has no cytotoxic effects on both cell lines whereas it induces a decrease in levels of VEGF and five proinflammatory cytokines (CCL2, CXCL8, CXCL10, IFN-γ, and TNF-α) only in MCF-7 cells in a dose-dependent manner, thereby indicating an anti-inflammatory effect. Moreover, interactomic analysis suggests that all six cytokines are involved in a network and are connected with some HUB nodes such as NF-kB subunits and ESR1 (estrogen receptor 1). We also report a decrease in the expression of two NFKB1 and c-Rel subunits by RT-qPCR experiments only in MCF-7 cells after extract treatment, confirming NF-kB inactivation. These data highlight the potential ofG. cydoniumfor future drug discovery against major diseases, such as breast cancer.

Sign in / Sign up

Export Citation Format

Share Document