scholarly journals Anti-Inflammatory Effects of a Methanol Extract from the Marine SpongeGeodia cydoniumon the Human Breast Cancer MCF-7 Cell Line

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Susan Costantini ◽  
Giovanna Romano ◽  
Fabiola Rusolo ◽  
Francesca Capone ◽  
Eliana Guerriero ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Methanol Extract ◽  
Marine Sponges ◽  
Biologically Active ◽  
Dependent Manner ◽  
Human Breast ◽  
Normal Human Breast ◽  
Anti Inflammatory ◽  
Mcf 7

Many research groups are working to find new possible anti-inflammatory molecules, and marine sponges represent a rich source of biologically active compounds with pharmacological applications. In the present study, we tested different concentrations of the methanol extract from the marine sponge,Geodia cydonium, on normal human breast epithelial cells (MCF-10A) and human breast cancer cells (MCF-7). Our results show that this extract has no cytotoxic effects on both cell lines whereas it induces a decrease in levels of VEGF and five proinflammatory cytokines (CCL2, CXCL8, CXCL10, IFN-γ, and TNF-α) only in MCF-7 cells in a dose-dependent manner, thereby indicating an anti-inflammatory effect. Moreover, interactomic analysis suggests that all six cytokines are involved in a network and are connected with some HUB nodes such as NF-kB subunits and ESR1 (estrogen receptor 1). We also report a decrease in the expression of two NFKB1 and c-Rel subunits by RT-qPCR experiments only in MCF-7 cells after extract treatment, confirming NF-kB inactivation. These data highlight the potential ofG. cydoniumfor future drug discovery against major diseases, such as breast cancer.

scholarly journals Cytotoxic activity of crude saponins from Gaultheria trichophylla against human breast cancer cells MCF-7 and MDA-MB-468

2015 ◽  
Vol 10 (2) ◽  
pp. 443
Author(s):  
Fiaz Alam ◽  
Qazi Najam us Saqib ◽  
Abdul Waheed
Keyword(s):  
Breast Cancer ◽  
Cell Viability ◽  
Crude Extract ◽  
Human Breast Cancer ◽  
Neutral Red ◽  
Breast Cancer Cell Lines ◽  
Dependent Manner ◽  
Human Breast ◽  
Uptake Assay ◽  
Mcf 7

<p>This study was conducted to evaluate <em>Gaultheria trichophylla</em> crude extract and respective saponins fraction against human breast cancer cell lines. In MTT assay, cell viability was inhibited by <em>G. trichophylla</em> crude extract (500 µg/mL) and saponins (200 µg/mL) in a dose dependent manner with maximum inhibition of (82% and 85%) and (71% and 42%) against MCF-7 and MDA MB-468, respectively. In neutral red uptake assay, the cell viability was inhibited by crude extract and saponins (100 µg/mL) in a similar manner with maximum inhibitions of (96% and 93%) and (87% and 61%) against MCF-7 and MDA MB-468, respectively, with respect to 91% and 93% inhibition by actinomycin-D (4 µM). The DAPI (4',6-diamidino-2-phenylindole) (10 µg/mL) staining of MCF-7 cells treated with crude saponins showed shrunken nuclei with apparent nuclear fragmentation indicating apoptosis and in contrast, MDA MB-468 showed necrosis mode of cell death. The study exhibited that the <em>G. trichophylla</em> provides new evidences to further explore this plant for the novel targets in anticancer drug development.</p>

scholarly journals Formulation and Development of Transferrin Targeted Solid Lipid Nanoparticles for Breast Cancer Therapy

2020 ◽  
Vol 11 ◽  
Author(s):  
Geeta S. Bhagwat ◽  
Rajani B. Athawale ◽  
Rajeev P. Gude ◽  
Shadab Md ◽  
Nabil A. Alhakamy ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Solid Lipid Nanoparticles ◽  
Human Breast Cancer ◽  
Lipid Nanoparticles ◽  
Dependent Manner ◽  
Human Breast ◽  
Solid Lipid ◽  
Receptor Modulation ◽  
Tamoxifen Citrate ◽  
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Breast cancer is conventionally treated by surgery, chemotherapy and radiation therapy followed by post operational hormonal therapy. Tamoxifen citrate is a best option to treat breast cancer because its selective estrogen receptor modulation activity. Owing to its antiestrogenic action on breast as well as uterine cells, Tamoxifen citrate shows uterine toxicity. The dose 20 mg per day of Tamoxifen citrate required to show therapeutic effect causes side effects and toxicity to vital organs such as liver, kidney and uterus. In the present study, transferrin-conjugated solid lipid nanoparticles (SLNs) were successfully prepared to enhance the active targeting of tamoxifen citrate in breast cancer. Developed formulations were evaluated for particle size, surface charge, surface morphology and in vitro dissolution studies. Developed formulations exhibited more cytotoxicity as compared to pure Tamoxifen citrate solution in time as well as concentration dependent manner on human breast cancer MCF-7 cells. Further, cell uptake and flow cytometry studies confirmed the qualitative uptake of developed D-SLN and SMD-SLN by human breast cancer MCF-7 cells. Overall, proposed study highlights that transferrin engineered nanocarriers could enhance the therapeutic response of nanomedicines for breast cancer treatment.

Furanodiene alters mitochondrial function in doxorubicin-resistant MCF-7 human breast cancer cells in an AMPK-dependent manner

2016 ◽  
Vol 12 (5) ◽  
pp. 1626-1637 ◽  
Author(s):  
Zhang-Feng Zhong ◽  
Wen Tan ◽  
William W. Qiang ◽  
Virginia L. Scofield ◽  
Ke Tian ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Chinese Medicine ◽  
Cancer Therapy ◽  
Traditional Chinese Medicine ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Dependent Manner ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7

Furanodiene is a bioactive sesquiterpene isolated from the spice-producing Curcuma wenyujin plant (Y. H. Chen and C. Ling) (C. wenyujin), which is a commonly prescribed herb used in clinical cancer therapy by modern practitioners of traditional Chinese medicine.

GC-MS Identification of Anti-inflammatory and Anticancer Metabolites in Edible Milky White Mushroom (Calocybe indica) against Human Breast Cancer (MCF-7) Cells

2021 ◽  
pp. 4300-4306
Author(s):  
Mohanasundaram S. ◽  
Rangarajan N. ◽  
Sampath V. ◽  
Porkodi K. ◽  
M.V. Dass Prakash ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Protein Denaturation ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Inhibition Assay ◽  
Human Breast ◽  
Anti Inflammatory ◽  
Calocybe Indica ◽  
Mcf 7

Milky White Mushroom is a species of edible mushroom native to India. The Purpose of this study was to determine the anti-inflammatory and anticancer compounds from Calocybe indica. The ethanolic extract from Calocybe indica were prepared. GC-MS was performed and the major bioactive compounds such as polysaccharides, amino acids, sterols, phytol, and squalene were determined. Calocybe indica was analyzed for their antioxidant activity through In vitro assays such as Free radical activity on DPPH, Hydroxyl radical scavenging assay, Nitric Oxide scavenging assay, Superoxide scavenging assay. Anti-inflammatory effect was estimated by protein denaturation inhibition assay, Proteinase inhibition assay, Cyclooxygenase inhibition assay and Lipoxygenase inhibition assay. Anticancer activity of Calocybe indica extract showed maximum inhibition of 69.11% of growth of human breast cancer cell (MCF 7) at 100μg/ml exposure for about 72 hours. At the end of this study, it was indicated that ethanolic extract of Calocybe indica can be used as an anti- inflammatory and anticancer agent.

Kru¨ ppel-like factor 5 in human breast carcinoma: a potent prognostic factor induced by androgens

2020 ◽  
Author(s):  
Lungwani Muungo
Keyword(s):  
Breast Cancer ◽  
Prognostic Factor ◽  
Breast Carcinoma ◽  
Carcinoma Cells ◽  
Biologically Active ◽  
Dependent Manner ◽  
Breast Cancer Patients ◽  
Human Breast ◽  
Cancer Specific Survival ◽  
Mcf 7

Kru¨ ppel-like factor 5 (intestinal) or Kru¨ ppel-like factor 5 (KLF5) is a zinc finger-containingtranscription factor and involved in important biological processes including cell proliferation anddifferentiation. However, clinical significance of KLF5 protein has remained largely unknown inbreast cancer. Therefore, in this study, we immunolocalized KLF5 in 113 human breast carcinomacases. KLF5 immunoreactivity was frequently detected in the nuclei of breast carcinoma cells, andmedian value of the ratio of KLF5-positive carcinoma cells was 30% and was positively associatedwith the status of androgen receptor. KLF5 immunoreactivity was also significantly associated withincreased risk of recurrence and worse clinical outcome in breast cancer patients by univariateanalyses, and subsequent multivariate analyses demonstrated that KLF5 immunoreactivity was anindependent prognostic factor for both disease-free and breast cancer-specific survival of thepatients. We then examined possible regulation of KLF5 by androgen using MCF-7 breastcarcinoma cells. KLF5 mRNA was induced by biologically active androgen 5a-dihydrotestosteronein a dose- and time-dependent manner in MCF-7 cells. In addition, results of transfectionexperiments demonstrated that proliferation activity of MCF-7 cells was significantly associatedwith the KLF5 expression level. These findings suggest that KLF5 is an androgen-responsive genein humanbreast carcinomas and play important roles in the progression of breast carcinomas. KLF5immunoreactivity is therefore considered a potent prognostic factor in human breast cancers.Endocrine-Related Cancer (2012) 19 741–750

Inhibitory Effect of Hydroxysafflor Yellow B on the Proliferation of Human Breast Cancer MCF-7 Cells

2019 ◽  
Vol 14 (2) ◽  
pp. 187-197 ◽  
Author(s):  
Chuanjun Qu ◽  
Weiwei Zhu ◽  
Kaijie Dong ◽  
Zhaohai Pan ◽  
Ying Chen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Flow Cytometry ◽  
Cell Apoptosis ◽  
Human Breast Cancer ◽  
S Phase ◽  
Dependent Manner ◽  
Human Breast ◽  
Protein Levels ◽  
Mcf 7

Background:A recent patent has been issued for hydroxysafflor yellow A (HSYA) as a drug to prevent blood circulation disorders. Hydroxysafflor yellow B (HSYB), an isomer of HSYA with antioxidative effects, has been isolated from the florets of Carthamus tinctorius. The effects of HSYB on the proliferation of cancer cells and its mechanism of action have not been investigated.Objective:The aims of this study were to investigate the anti-cancer effects and the molecular mechanism of HSYB for breast cancer MCF-7 cells.Methods:MTT assays and colony formation assays were used to assess the survival and proliferation of MCF-7 cells, respectively. Hoechst 33258 and flow cytometry were used to measure cell apoptosis and flow cytometry to determine effects on the cell cycle. Western blots were used to measure protein levels.Results:Treatment with HSYB reduced survival and proliferation of human breast cancer MCF-7 cells in a dose-dependent manner. Furthermore, HSYB arrested the MCF-7 cell cycle at the S phase and downregulated cyclin D1, cyclin E, and CDK2. Compared with a control group, HSYB suppressed the protein levels of p-PI3K, PI3K, AKT, and p-AKT in MCF-7 cells. In addition, HSYB decreased the levels of Bcl- 2, increased the levels of Bax, cleaved caspase-3 and caspase-9, and subsequently induced MCF-7 cell apoptosis.Conclusion:These data demonstrate that HSYB arrests the MCF-7 cell cycle at the S phase and induces cell apoptosis. Patent US20170246228 indicates that HSYB can be potentially used for the prevention and treatment of human breast cancer.

scholarly journals Emodin Inhibits the Proliferation of MCF-7 Human Breast Cancer Cells Through Activation of Aryl Hydrocarbon Receptor (AhR)

2021 ◽  
Vol 11 ◽  
Author(s):  
Ning Zhang ◽  
Jiawen Wang ◽  
Aimin Sheng ◽  
Shuo Huang ◽  
Yanyan Tang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Aryl Hydrocarbon Receptor ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Dependent Manner ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Aryl Hydrocarbon ◽  
Mcf 7

Natural products have proved to be a promising source for the development of potential anticancer drugs. Emodin, a natural compound from Rheum palmatum, is used to treat several types of cancers, including lung, liver, and pancreatic. However, there are few reports regarding its use in the treatment of breast cancer. Thus, the therapeutic effect and mechanism of emodin on MCF-7 human breast cancer cells were investigated in this study. Morphological observations and cell viability were evaluated to determine the anti-proliferation activity of emodin. Network pharmacology and molecular docking were performed to screen the potential targets. Western blot analysis was used to explore a potential antitumor mechanism. The results showed that emodin (50–100 μmol/L) could significantly inhibit the proliferation of MCF-7 cells in a time and dose-dependent manner. Furthermore, virtual screening studies indicated that emodin was a potent aryl hydrocarbon receptor (AhR) agonist in chemotherapy for breast cancer. Finally, when MCF-7 cells were treated with emodin (100 μmol/L) for 24 h, the AhR and cytochrome P450 1A1 (CYP1A1) protein expression levels were significantly upregulated compared with the control group. Our study indicated that emodin exhibited promising antitumor activity in MCF-7 cells, likely through activation of the AhR-CYP1A1 signaling pathway. These findings lay a foundation for the application of emodin in breast cancer treatment.

scholarly journals Roles of JNK/Nrf2 Pathway on Hemin-Induced Heme Oxygenase-1 Activation in MCF-7 Human Breast Cancer Cells

Medicina ◽  
2020 ◽  
Vol 56 (6) ◽  
pp. 268
Author(s):  
Hye-Yeon Jang ◽  
On-Yu Hong ◽  
Eun-Yong Chung ◽  
Kwang-Hyun Park ◽  
Jong-Suk Kim
Keyword(s):  
Breast Cancer ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Heme Oxygenase ◽  
Human Breast Cancer ◽  
Human Breast Cancer Cell ◽  
Heme Oxygenase 1 ◽  
Dependent Manner ◽  
Human Breast ◽  
Mcf 7

Heme oxygenase-1 (HO-1) is highly induced in various human disease states, including cancer, indicating that HO-1 is an emerging target of cancer therapy. In this study, we investigated that the mechanisms of hemin-induced HO-1 expression and its signaling pathways in human breast cancer cell. We used MCF-7 cells, a human breast cancer cell line. Hemin increased HO-1 expression in MCF-7 cells in a dose- and time-dependent manner. Hemin enhanced HO-1 expression through the activation of c-Jun N-terminal kinases (JNK) signaling pathway. Hemin also induced activation of Nrf2, a major transcription factor of HO-1 expression. These responses in MCF-7 cells were completely blocked by pretreatment with brazilin, a HO-1 regulator. These results indicated that brazilin inhibits hemin-induced HO-1 expressions through inactivation of JNK/Nrf2 in MCF-7 cells. Thus, our findings suggest that HO-1 is an important anticancer-target of brazilin in human breast cancer.

In vitro anti-inflammatory and anti-proliferative potential of roots extract of Euphorbia hirta Linn

2021 ◽  
Vol 10 (1) ◽  
pp. 7-9
Author(s):  
Rashmi ◽  
◽  
Pankaj Gupta ◽  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Human Breast Cancer ◽  
Protein Denaturation ◽  
Alcoholic Extract ◽  
Human Breast ◽  
Euphorbia Hirta ◽  
Proliferative Effect ◽  
Anti Inflammatory ◽  
Mcf 7

Aim: The main aim of this current study was to characterize the anti-inflammatory and anti-proliferative activities of alcoholic extract of the roots of euphorbia hirta. Genus Euphorbia contains many therapeutic plants which are widely studied. This study is designed to explore the effects of alcoholic roots extract of euphorbia hirta, on selected cell lines of Human Breast cancer (MCF - 7). Methods: The Human Breast Cancer cell lines i.e. MCF – 7 cell lines were treated with alcoholic roots extract of euphorbia hirta at different concentrations. Anti-proliferative effect of the alcoholic extract was evaluated by assessing the cell viability. MTT assay was used to determine the cell viability at the wavelength of 570 nm. Antiinflammatory potential of extract was evaluated by calculating the percentage inhibition of protein denaturation. Results: The results showed the significant anti-inflammatory potential as it effectively inhibits the protein denaturation. The alcoholic roots extract of euphorbia hirta also showed moderate cytotoxic effect against MCF - 7 cell-lines (P ≤0.05). Conclusions: The results showed that this extract had significant anti-inflammatory potential and moderate anti-proliferative effect, as extract was able to inhibit the proliferation of tumor cells. Hence, it seems to be a virtuous contender to protect against growth of tumor cells.

Oridonin Induces Apoptosis, Inhibits Migration and Invasion on Highly-Metastatic Human Breast Cancer Cells

2013 ◽  
Vol 41 (01) ◽  
pp. 177-196 ◽  
Author(s):  
Shengpeng Wang ◽  
Zhangfeng Zhong ◽  
Jianbo Wan ◽  
Wen Tan ◽  
Guosheng Wu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Migration And Invasion ◽  
Dependent Manner ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Integrin Β1 ◽  
Mcf 7

Oridonin, a natural tetracycline diterpenoid isolated from Chinese herb Rabdosia rubescens, has been reported to be a potent cytotoxic agent against a wide variety of tumors. However, its effect on highly metastatic breast cancer cells has not been addressed. In this study, we investigated the effects of oridonin on growth, migration and invasion of highly-metastatic human breast cancer cells. Our results showed that oridonin induced potent growth inhibition on human breast cancer cells MCF-7 and MDA-MB-231 in a time- and dose-dependent manner. According to the flow cytometric analysis, oridonin suppressed MCF-7 cell growth by cell cycle arrest at the G2/M phase and caused accumulation of MDA-MB-231 cells in the Sub-G1 phase. The induced apoptotic effect of oridonin was further confirmed by a morphologic characteristics assay and TUNEL assay. Oridonin triggered the reduction of Bcl-2/Bax ratio, caspase-8, NF-κB (p65), IKKα, IKKβ, phospho-mTOR, and increased expression level of cleaved PARP, Fas and PPARγ in a time-dependent manner. Immunofluorescent analysis showed that γH2AX-containing nuclear foci were significant in oridonin-treated MDA-MB-231 cells. Meanwhile, oridonin significantly suppressed MDA-MB-231 cell migration and invasion, decreased MMP-2/MMP-9 activation and inhibited the expression of Integrin β1 and FAK. In conclusion, oridonin inhibited the growth and induced apoptosis in breast cancer cells, which might be related to DNA damage and activation of intrinsic or extrinsic apoptotic pathways. Moreover, oridonin also inhibited tumor invasion and metastasis in vitro possibly via decreasing the expression of MMPs and regulating the Integrin β1/FAK pathway in MDA-MB-231 cells.

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