Sensitization to inhalant and food allergens in Brazilian atopic children by <i>in vitro</i> total and specific IgE assay. Allergy Project - PROAL

10.2223/1184 ◽  
2004 ◽  
Vol 80 (3) ◽  
pp. 203-210 ◽  
Author(s):  
Charles K. Naspitz ◽  
Dirceu Solé ◽  
Cristina A. Jacob ◽  
Emanuel Sarinho ◽  
Francisco J. P. Soares ◽  
...  
Keyword(s):  
Specific Ige ◽  
Food Allergens

scholarly journals Frequency and Pattern of IgE-mediated Sensitization to Aero and Food Allergens in Ahvaz, Province of Khuzestan in Southwestern Iran

2018 ◽  
Author(s):  
Mohammad Shahrooei ◽  
Abbas Fayezi ◽  
Raheleh Shokouhi Shoormasti ◽  
Faramarz Zakavi ◽  
Leila Golpasand Hagh ◽  
...  
Keyword(s):  
Medical Records ◽  
Clinical Symptoms ◽  
Specific Ige ◽  
Proper Treatment ◽  
Food Allergens ◽  
Treatment Need ◽  
Ige Mediated ◽  
Southwestern Iran ◽  
Ige Determination

Efficient diagnosis of allergy and proper treatment need identification of the causative allergens eliciting clinical symptoms. The present study was performed to identify the most common aero- and food allergens and determine the pattern of sensitization among people of Ahvaz (southwestern Iran), one of the most polluted cities worldwide. Based on the physical examination and medical records, patients were referred to the Allergy laboratory for “in vitro” IgE determination. Specific and total IgE was determined by the ImmunoCAP system (Thermo Fisher-Phadia, Uppsala, Sweden). A total of 666 consecutive patients (51.1% female) were tested for 202 different allergens. The majority of requests (57%) belonged to food allergens. Sensitization to at least one allergen was found in 47.6% of patients. In a selected group of allergens for which specific IgE had been tested in at least 100 patients, the most common sensitizing aeroallergens were Russian thistle, grass pollen, and willow; while wheat, honey, and shrimp were the most frequent food allergens, respectively. Sensitization profiles based on measurement of specific IgE indicated that Russian thistle, grasses, and wheat were the most prevalent allergens in people with allergic symptoms living in Ahvaz.

scholarly journals Sensitization to inhalant and food allergens in Brazilian atopic children by in vitro total and specific IgE assay. Allergy Project - PROAL

2004 ◽  
Vol 80 (3) ◽  
pp. 203-10 ◽  
Author(s):  
Charles K. Naspitz ◽  
Dirceu Sol&eacute; ◽  
Cristina A. Jacob ◽  
Emanuel Sarinho ◽  
Francisco J. P. Soares ◽  
...  
Keyword(s):  
Specific Ige ◽  
Food Allergens

scholarly journals Oral Allergy Syndrome in a Child Provoked by Royal Jelly

2014 ◽  
Vol 2014 ◽  
pp. 1-3 ◽  
Author(s):  
Fantini Paola ◽  
Delle Donne Pantalea ◽  
Calogiuri Gianfranco ◽  
Ferrannini Antonio ◽  
Vacca Angelo ◽  
...  
Keyword(s):  
Patch Test ◽  
Royal Jelly ◽  
Specific Ige ◽  
Oral Allergy Syndrome ◽  
Food Allergens ◽  
Lipid Transfer ◽  
Prick Test ◽  
Inhalant Allergens

Royal jelly has been demonstrated to have several physiological activities. However, in the literature, different reactions induced by royal jelly are reported. We describe a case of seven-year-old child that was referred to our observation for two episodes of oral allergy syndrome (OAS) that appeared ten minutes after ingestion of royal jelly. Skin prick test with standard panel of inhalant and food allergens, a prick-to-prick test using the royal jelly’s extract responsible for patient’s reactions, and royal jelly patch test with extemporaneous preparation were performed. The specific IgE by ImmunoCAP System method versus Hymenoptera venom, inhalant allergens, food allergens, and lipid transfer proteins was dosed. According to the positive reactions to royal jelly both by prick-by-prick test and by a first reading patch test, royal jelly immediate hypersensitivity was diagnosed. According to the positive response for almond in bothin vivoandin vitrotests we can think of the royal jelly contamination with almond pollen as possible cause of patient’s reaction. Moreover, from the results of specific IgE titers versus Compositae pollens, we have argued the possibility that this case of royal jelly allergy could be explained also by the mechanism of cross-reaction with Compositae pollens.

scholarly journals The Importance of Diet in Predicting the Remission of Urticaria—Determination of Allergen-Specific IgE

Medicina ◽  
2021 ◽  
Vol 57 (7) ◽  
pp. 679
Author(s):  
Monica Iuliana Ungureanu ◽  
Liliana Sachelarie ◽  
Radu Ciorap ◽  
Bogdan Aurelian Stana ◽  
Irina Croitoru ◽  
...  
Keyword(s):  
Predictive Value ◽  
Allergic Diseases ◽  
Specific Ige ◽  
Egg White ◽  
Food Allergens ◽  
Pediatric Hospital ◽  
Disease Remission ◽  
Specific Serum ◽  
Different Types

Background and Objectives: Different types of food introduced gradually in the diet will expose children to different food allergens, increasing the chance of developing allergic diseases. The aim of our study was to determine if allergen-specific IgE values can influence, depending on the diet, the prediction of remission of urticaria in children. Materials and Methods: This prospective study was conducted in 132 patients diagnosed over two years with urticaria, admitted to “Sf. Maria” Clinical Pediatric Hospital Iaşi. Total IgE assay was performed by ELISA, and determination of specific serum IgE by the CLA System Quanti Scan method (Innogenetics, Heiden, Germany). Data were gathered and statistical analysis was performed using statistical software SPSS, using descriptive and inferential statistics. Results: The determination of specific IgE to food allergens was performed on a total of 132 cases. The values of specific IgE were positive for one or more food allergens in 84 patients (63.64%). The most common allergens involved were: cow’s milk in 33.3% cases, egg white in 22.6% cases, and hazelnuts in 11.9% cases. The specific IgE values for the different types of food included in our study had a predictive value for disease remission. Conclusions: The determination of specific IgE confirms the presence of a particular food allergen and may have predictive value for the future development of an allergic manifestation.

In vitro detection of specific IgE antibodies to erythromycin☆☆☆★★★

1995 ◽  
Vol 95 (3) ◽  
pp. 668-671 ◽  
Author(s):  
Cristina Pascual ◽  
Jesus F. Crespo ◽  
Joaquin Quiralte ◽  
Concepcion Lopez ◽  
Gary Wheeler ◽  
...  
Keyword(s):  
Specific Ige ◽  
Ige Antibodies ◽  
Specific Ige Antibodies

scholarly journals Recent advances in allergic rhinitis

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1333 ◽  
Author(s):  
Flavia C. L. Hoyte ◽  
Harold S. Nelson
Keyword(s):  
Allergic Rhinitis ◽  
Work Productivity ◽  
Allergen Challenge ◽  
Economic Cost ◽  
Symptomatic Treatment ◽  
The United States ◽  
Specific Ige ◽  
Subcutaneous Immunotherapy ◽  
Nasal Allergy

Allergic rhinitis affects 20 to 30% of adults in both the United States and Europe and perhaps a somewhat higher percentage of children. In addition to nasal and ocular symptoms directly related to the allergic process, interference of these symptoms with sleep leads to daytime sleepiness and impaired quality of life. Patients miss work because of symptoms but an even greater problem is interference with work productivity, or presenteeism, which has been reported to be the biggest contributor to the total economic cost of allergic rhinitis. There has been increasing awareness that many patients with either seasonal or perennial symptoms but negative skin and in vitro tests for allergen sensitivity have local nasal allergy, diagnosable by the presence of allergen-specific IgE in their nasal secretions or a positive nasal allergen challenge or both. The pharmaceutical management of allergic rhinitis rests on symptomatic treatment with antihistamines that perhaps are more effectively administered intranasally than orally and intranasal corticosteroids. Allergen immunotherapy is very effective, even for local allergic rhinitis, and the shortcomings of subcutaneous immunotherapy of inconvenience and safety are reduced by the introduction of sublingual immunotherapy (SLIT). Use of the latter is currently somewhat limited by the lack of appropriate dosing information for SLIT liquids and the limited number of allergens for which SLIT tablets are available.

Hymenolepis murissylvatici: humoral response in intestinal lavages of the mouse

1989 ◽  
Vol 63 (1) ◽  
pp. 25-31 ◽  
Author(s):  
E. Van Der Vorst ◽  
H. Dhont ◽  
C. Van Haeren ◽  
R. Deceunynck ◽  
P. H. De Rycke
Keyword(s):  
Tissue Damage ◽  
Humoral Response ◽  
Specific Ige ◽  
Immune Serum ◽  
Apparent Effect ◽  
Igm Antibodies ◽  
Immunological Reactions

ABSTRACTHymenolepis murissylvatici elicits a humoral response in serum and in the intestine of the mouse from which it is immunologically rejected. In serum, significant differences were recorded 3 days after reinfection, while in intestinal lavages it takes place from day 9 after reinfection. In serum the response is largely the result of IgG and IgM antibodies whereas in the intestine, IgA is the most abundant antibody. Although specific IgE could not be demonstrated in serum, it was present in intestinal lavages, although rather late (i.e. day 14 after reinfection). Treatment of young worms in vitro both with immune serum or intestinal lavages had no apparent effect on their viability. Immune serum produced a complement independent precipitation on the surface of the worms in vitro. This reaction did not affect viability or infectivity of the parasite, as demonstrated by the successful implantation of treated worms in recipient mice. The above-mentioned results, together with the knowledge that the Hymenolepis model has no tissue stages and causes no tissue damage, make it probable that further study of this model will reveal some specific intestinal immunological reactions.

scholarly journals Neo-antigens for the serological diagnosis of IgE-mediated drug allergic reactions to antibiotics cephalosporin, carbapenem and monobactam

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Edurne Peña-Mendizabal ◽  
Sergi Morais ◽  
Ángel Maquieira
Keyword(s):  
Drug Allergy ◽  
High Sensitivity ◽  
Specific Ige ◽  
In Vitro Tests ◽  
Allergic Reactions ◽  
Serum Samples ◽  
Lactam Ring ◽  
Specific Igg ◽  
Ige Mediated

Abstract New antigens deriving from -lloyl and -llanyl, major and minor determinants, respectively, were produced for β-lactam antibiotics cefuroxime, cefotaxime, ceftriaxone, meropenem and aztreonam. Twenty β-lactam antigens were produced using human serum albumin and histone H1 as carrier proteins. Antigens were tested by multiplex in vitro immunoassays and evaluated based on the detection of specific IgG and IgE in the serum samples. Both major and minor determinants were appropriate antigens for detecting specific anti-β-lactam IgG in immunised rabbit sera. In a cohort of 37 allergic patients, we observed that only the minor determinants (-llanyl antigens) were suitable for determining specific anti-β-lactam IgE antibodies with high sensitivity (< 0.01 IU/mL; 24 ng/L) and specificity (100%). These findings reveal that not only the haptenisation of β-lactam antibiotics renders improved molecular recognition events when the 4-member β-lactam ring remains unmodified, but also may contribute to develop promising minor antigens suitable for detecting specific IgE-mediated allergic reactions. This will facilitate the development of sensitive and selective multiplexed in vitro tests for drug-allergy diagnoses to antibiotics cephalosporin, carbapenem and monobactam.

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