Diagnostic performance of various in vitro assays for specific IgE in adults allergic to natural rubber latex*1

2004 ◽  
Vol 113 (2) ◽  
pp. S136
Author(s):  
M OLLERT
Keyword(s):  
Natural Rubber ◽  
Diagnostic Performance ◽  
Natural Rubber Latex ◽  
Specific Ige ◽  
In Vitro Assays ◽  
Rubber Latex

Measurement of natural rubber latex allergen levels in medical gloves by allergen-specific IgE-ELISA inhibition, RAST inhibition, and skin prick test

Allergy ◽  
1998 ◽  
Vol 53 (1) ◽  
pp. 59-67 ◽  
Author(s):  
T. Palosuo ◽  
S. Mäkinen-Kiljunen ◽  
H. Alenius ◽  
T. Reunala ◽  
E. Yip ◽  
...  
Keyword(s):  
Natural Rubber ◽  
Skin Prick Test ◽  
Natural Rubber Latex ◽  
Specific Ige ◽  
Rubber Latex ◽  
Prick Test ◽  
Elisa Inhibition

Characterization of Lidocaine Transdermal Patches from Natural Rubber Latex

2013 ◽  
Vol 747 ◽  
pp. 103-106 ◽  
Author(s):  
Prapaporn Boonme ◽  
Hasleena Boontawee ◽  
Wirach Taweepreda ◽  
Wiwat Pichayakorn
Keyword(s):  
Drug Release ◽  
Natural Rubber ◽  
Physicochemical Properties ◽  
Rubber Tree ◽  
Natural Rubber Latex ◽  
Rubber Latex ◽  
Transdermal Patches ◽  
The Stability ◽  
Main Component

The mucous liquid of Hevea brasiliensis or Para rubber tree, called natural rubber latex (NRL), composes of cis-1,4-polyisoprene which can form a patch under suitable formulation. In this study, blank and 5% lidocaine-loaded NRL patches were formulated and then characterized for physicochemical properties as well as evaluated in vitro drug release and stability. The patches were observed for their appearances. Surface morphology of the patches was investigated using a SEM. XRD was employed to study the crystallinity of the drug, the patch, and the drug-loaded patch. The extractions of lidocaine-loaded patches were analyzed for drug contents by HPLC. In vitro drug release study was performed using modified Franz diffusion cells. The patches at initial preparation and after kept at 4, 25, and 45 °C for 3 months were investigated for the stability determination. The results suggested that NRL could be used as a main component in pharmaceutical transdermal patches with acceptable physicochemical properties. Lidocaine-loaded NRL patches provided desirable drug release but high storage temperatures could age the patches resulting in darken color and lower release amount.

scholarly journals Novel Type of Heme-Dependent Oxygenase Catalyzes Oxidative Cleavage of Rubber (Poly-cis-1,4-Isoprene)

2004 ◽  
Vol 70 (12) ◽  
pp. 7388-7395 ◽  
Author(s):  
Reinhard Braaz ◽  
Peter Fischer ◽  
Dieter Jendrossek
Keyword(s):  
Natural Rubber ◽  
High Performance ◽  
Natural Rubber Latex ◽  
Culture Fluid ◽  
Oxidative Cleavage ◽  
Covalent Attachment ◽  
Rubber Latex ◽  
Chromatography Analysis ◽  
Cloned Gene

ABSTRACT An extracellular protein with strong absorption at 406 nm was purified from cell-free culture fluid of latex-grown Xanthomonas sp. strain 35Y. This protein was identical to the gene product of a recently characterized gene cloned from Xanthomonas sp., as revealed by determination of m/z values and sequencing of selected isolated peptides obtained after trypsin fingerprint analysis. The purified protein degraded both natural rubber latex and chemosynthetic poly(cis-1,4-isoprene) in vitro by oxidative cleavage of the double bonds of poly(cis-1,4-isoprene). 12-Oxo-4,8-dimethyltrideca-4,8-diene-1-al (m/z 236) was identified and unequivocally characterized as the major cleavage product, and there was a homologous series of minor metabolites that differed from the major degradation product only in the number of repetitive isoprene units between terminal functions, CHO-CH2— and —CH2-COCH3. An in vitro enzyme assay for oxidative rubber degradation was developed based on high-performance liquid chromatography analysis and spectroscopic detection of product carbonyl functions after derivatization with dinitrophenylhydrazone. Enzymatic cleavage of rubber by the purified protein was strictly dependent on the presence of oxygen; it did not require addition of any soluble cofactors or metal ions and was optimal around pH 7.0 at 40°C. Carbon monoxide and cyanide inhibited the reaction; addition of catalase had no effect, and peroxidase activity could not be detected. The purified protein was specific for natural rubber latex and chemosynthetic poly(cis-1,4-isoprene). Analysis of the amino acid sequence deduced from the cloned gene (roxA [rubber oxygenase]) revealed the presence of two heme-binding motifs (CXXCH) for covalent attachment of heme to the protein. Spectroscopic analysis confirmed the presence of heme, and approximately 2 mol of heme per mol of RoxA was found.

Natural rubber latex-specific IgE antibodies in non-healthcare workers: comparison of two FDA-clearedin vitro kits

1999 ◽  
Vol 1 (3) ◽  
pp. 147-151 ◽  
Author(s):  
Raymond E. Biagini ◽  
Barbara A. Mackenzie ◽  
Toni A. Bledsoe ◽  
Daniel M. Lewis ◽  
Lynne M. Pinkerton
Keyword(s):  
Natural Rubber ◽  
Healthcare Workers ◽  
Natural Rubber Latex ◽  
Specific Ige ◽  
Rubber Latex ◽  
Ige Antibodies ◽  
Specific Ige Antibodies
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