scholarly journals Oral Allergy Syndrome in a Child Provoked by Royal Jelly

2014 ◽  
Vol 2014 ◽  
pp. 1-3 ◽  
Author(s):  
Fantini Paola ◽  
Delle Donne Pantalea ◽  
Calogiuri Gianfranco ◽  
Ferrannini Antonio ◽  
Vacca Angelo ◽  
...  
Keyword(s):  
Patch Test ◽  
Royal Jelly ◽  
Specific Ige ◽  
Oral Allergy Syndrome ◽  
Food Allergens ◽  
Lipid Transfer ◽  
Prick Test ◽  
Inhalant Allergens

Royal jelly has been demonstrated to have several physiological activities. However, in the literature, different reactions induced by royal jelly are reported. We describe a case of seven-year-old child that was referred to our observation for two episodes of oral allergy syndrome (OAS) that appeared ten minutes after ingestion of royal jelly. Skin prick test with standard panel of inhalant and food allergens, a prick-to-prick test using the royal jelly’s extract responsible for patient’s reactions, and royal jelly patch test with extemporaneous preparation were performed. The specific IgE by ImmunoCAP System method versus Hymenoptera venom, inhalant allergens, food allergens, and lipid transfer proteins was dosed. According to the positive reactions to royal jelly both by prick-by-prick test and by a first reading patch test, royal jelly immediate hypersensitivity was diagnosed. According to the positive response for almond in bothin vivoandin vitrotests we can think of the royal jelly contamination with almond pollen as possible cause of patient’s reaction. Moreover, from the results of specific IgE titers versus Compositae pollens, we have argued the possibility that this case of royal jelly allergy could be explained also by the mechanism of cross-reaction with Compositae pollens.

scholarly journals Oral allergy syndrome by fruit and vegetable PR-10 allergy: Accuracy of in vivo diagnosis

2021 ◽  
Vol 49 (2) ◽  
pp. 129-132
Author(s):  
Cristina De Rose ◽  
Maria Letitzia Patti ◽  
Alessadro Gambacorta ◽  
Federica Brancato ◽  
Stefano Miceli Sopo
Keyword(s):  
Specific Ige ◽  
Fruit And Vegetables ◽  
Diagnostic Methods ◽  
Oral Allergy Syndrome ◽  
In Vitro Tests ◽  
Prick Test ◽  
Fresh Food ◽  
In Vivo Diagnosis

Routine diagnostic methods for allergies to plant-derived foods are based on skin prick test (SPT) with commercial extracts, prick-by-prick (PbP) with fresh food, serum-specific IgE measurement, and oral food challenge.We discuss the possibility and the advantages of performing, in patients with oral allergy syn-drome (OAS) by fruit and vegetables (excluding nuts) PR-10 allergy, component-resolved diag-nosis (CRD) by SPT and PbP with raw and cooked vegetables, rather than performing a CRD with in vitro tests by drawing blood.Based on our clinical experience and the studies published in the literature, we believe that, at least for the OAS by fruit and vegetables (excluding nuts) PR-10 allergy, the search for sensitizing allergens and related cross-reactive allergens with SPT and PbP can be performed routinely in clinical practice, even at the primary-care level.

scholarly journals Sublingual Reactivity to rBET V1 and rPHL P1 in Patients with Oral Allergy Syndrome

2006 ◽  
Vol 19 (1) ◽  
pp. 205873920601900 ◽  
Author(s):  
F. Marcucci ◽  
L. Sensi ◽  
G. DI Cara ◽  
G. Gidaro ◽  
C. Incorvaia ◽  
...  
Keyword(s):  
Specific Ige ◽  
Cross Reactivity ◽  
Oral Allergy Syndrome ◽  
Control Group ◽  
Recombinant Allergens ◽  
Natural Extracts ◽  
Target Organs ◽  
High Concentrations

Oral Allergy Syndrome (OAS) in patients with pollen-induced rhinoconjunctivitis is caused by specific IgE recognizing cross-reacting epitopes of fruits and plants, which were clearly shown in vitro, but failed to be demonstrated in vivo by cross-challenges in the target organs. Considering the hypothesis of degradation of such epitopes in natural extracts, challenges with recombinant pollen allergens were done to evaluate the reactivity of the oral mucosa in OAS patients. Seventeen patients with OAS and rhinitis from birch (10) and grass pollen (7) and 10 non-atopic controls were studied by skin prick tests (SPT), allergen specific nasal challenges (ASNC) and allergen specific sublingual challenges (ASSC) with birch and timothy extracts and with rBet v1 and rPhl p1 at increasing concentrations from 1 to 1000 mcg/ml. None of the healthy subjects in the control group had any positive test for birch and timothy extracts or for recombinant allergens. In the OAS group the following results were observed: SPTs with recombinant allergens were positive in all patients, mostly at 10 mcg/ml concentration; ASNC with rBet v1 were positive in all patients, mostly at 100 mcg/ml; ASSC with natural pollen extracts were positive in only 2 of 17 patients, but in 15 of 17 with rBet v1 and rPhl p1, mostly at 500 mcg/ml and 1000 mcg/ml. ASSC with rBet v1 and rPhl p1 were positive with a mean concentration of 677 and 533 mcg/ml, respectively. The results of sublingual challenges with rBet v1 and rPhl p1 showed the in vivo cross-reactivity between pollens and foods in patients with OAS, but high concentrations of the recombinant allergens were needed to reproduce oral symptoms, thus explaining the failure of challenges performed with natural extracts, which have concentrations of major allergens lower than 50 mcg/ml. This indicates that sublingual mucosa is much less reactive to allergens than other surfaces, such as skin and nasal mucosa, probably because of its anatomic and immunologic peculiarity.

Birch pollen associated soy allergy – possibilities in diagnostic and clinical relevance1)

2012 ◽  
Vol 36 (3) ◽  
Author(s):  
Susanne Beyer ◽  
Ulrich Sack ◽  
Regina Treudler
Keyword(s):  
Birch Pollen ◽  
Basophil Activation Test ◽  
Specific Ige ◽  
Oral Allergy Syndrome ◽  
Food Allergies ◽  
Food Allergens ◽  
Prick Test ◽  
The Past ◽  
Immunological Cross Reaction

AbstractBirch pollen allergic individuals frequently suffer from food allergies in the form of an oral allergy syndrome after eating pome and stone fruits. These complaints are based on an immunological cross-reaction between pollen and food allergens. In the past, it has been shown that many birch pollen allergic patients are additionally not able to tolerate high protein soy products. Some severe immediate type reactions to soy have been observed. The cause for these immediate type reactions to soy is a Bet v 1 cross-reactive soy allergen called Gly m 4.Using a collective of 73 birch pollen allergic patients with associated food allergy in Leipzig as an example, the results of a standardized questioning, prick-to-prick test with a soy drink, determination of specific IgE against rGly m 4, and basophil activation test with Gly m 4 are presented.We showed that commercially available prick test extracts and determination of specific IgE against soy bean mix/f14 are not appropriate to diagnose birch pollen associated soy allergy. Generally, soy sensitization could be proven when a prick-to-prick-test with a soy drink and determination of specific IgE against rGly m 4 were done. A positive prick-to-prick test with a soy drink was found in 79% (55/70) of the birch pollen allergic patients with 89% (65/73) showing specific IgE for rGly m 4 (CAP>1). Although not every sensitization was clinically relevant, every third patient with a proven soy sensitization was diagnosed with a clinically relevant allergy to soy.

Alatop: A New In Vitro Screening Test for Atopy

1994 ◽  
Vol 22 (6) ◽  
pp. 313-322
Author(s):  
M Yazicioǧlu ◽  
P Sayinbaş ◽  
Ü Öneş ◽  
A Saltik ◽  
M Tug˜rul
Keyword(s):  
Antibody Test ◽  
Atopic Disease ◽  
Specific Ige ◽  
Total Serum ◽  
Healthy Children ◽  
Serum Ige ◽  
Prick Test ◽  
Skin Prick Tests ◽  
Inhalant Allergens

The value of a new in vitro multiallergen IgE antibody test in the diagnosis of atopy in children was compared with that of skin-prick tests and total serum IgE level determination. Twenty children with clinical histories and symptoms of asthma, median age 5 years, and 20 healthy children, median age 5.5 years, were enrolled. Total serum IgE levels were evaluated as either normal or high by referring to kit values. Specific IgE antibodies to 12 different inhalant allergens were screened by the Alatop test. The accuracy figures for the tests compared with clinical diagnosis were 65.0%, 72.5% and 80.0%, for the total serum IgE determination, the Alatop test and the skin-prick test, respectively, and the other measures of clinical reliability showed a similar pattern. Although the skin-prick tests were the most sensitive and specific of the three, for screening atopic disease in children, the Alatop test provides a valuable alternative, and the combined use of skin-prick tests with the Alatop test will provide a more reliable screen than using any single test.

scholarly journals Unraveling Kiwifruit Allergy Diagnosis: Usefulness of the Current Diagnostic Tests

2021 ◽  
Vol 32 (2) ◽  
Author(s):  
CM D’Amelio ◽  
A Bernad ◽  
BE García-Figueroa ◽  
S Garrido-Fernández ◽  
J Azofra ◽  
...  
Keyword(s):  
Seed Proteins ◽  
Food Challenge ◽  
Major Allergen ◽  
Specific Ige ◽  
Diagnostic Techniques ◽  
Prick Test ◽  
Skin Prick Tests ◽  
The Impact

Objectives: To determine the usefulness of the in vitro and in vivo methods used in the diagnosis of kiwifruit allergy, focusing on the impact of the seed proteins on their sensitivity. Methods: Skin prick tests (SPTs) using different commercial extracts, homemade pulp and seed extracts, and prick-prick test with kiwifruit were performed on 36 allergic patients. The presence of specific IgE (sIgE) was assessed using ImmunoCAP (kiwifruit extract), ELISA (Act d 1, Act d 2), ISAC and FABER assays. Immunoblotting of seed extract was carried out, and a single blinded oral food challenge with whole seeds was performed in seed-sensitized subjects. Results: The prick prick test with kiwifruit demonstrated the highest diagnostic capacity (81.8% sensitivity and 94.1% specificity) among the in vivo tests. The sIgE levels measured by ImmunoCAP-kiwifruit extract showed a similar sensitivity to that of global ISAC and FABER (63.9%, 59.5% and 58.3%, respectively). Act d 1 was the major allergen, and sensitization to it was associated with positive sIgE to whole kiwifruit extract detected by ImmunoCAP (p <0.000). A positive SPT with kiwifruit seeds was associated with severe symptoms with kiwifruit (p = 0.019) as a marker of an advanced disease, but not with clinically relevant sensitization. The challenge to kiwifruit seeds performed on eight seed-sensitized patients resulted negative. Conclusions: Sensitization to Act d 1 is related to a positive result in conventional diagnostic techniques, whereas kiwifruit seed sensitization.

Sensitization to inhalant and food allergens in Brazilian atopic children by <i>in vitro</i> total and specific IgE assay. Allergy Project - PROAL

10.2223/1184 ◽  
2004 ◽  
Vol 80 (3) ◽  
pp. 203-210 ◽  
Author(s):  
Charles K. Naspitz ◽  
Dirceu Solé ◽  
Cristina A. Jacob ◽  
Emanuel Sarinho ◽  
Francisco J. P. Soares ◽  
...  
Keyword(s):  
Specific Ige ◽  
Food Allergens

Abstract 319: Dalcetrapib-Mediated Modulation of Cholesteryl Ester Transfer Protein Activity Increases HDL-Cholesterol, Apolipoprotein A-I Levels and Cholesterol Efflux Capacity in Chow-Fed Rabbits

2012 ◽  
Vol 32 (suppl_1) ◽  
Author(s):  
Mathieu R Brodeur ◽  
David Rhainds ◽  
Daniel Charpentier ◽  
Téodora Mihalache-Avram ◽  
Cyrille Maugeais ◽  
...  
Keyword(s):  
Cholesteryl Ester ◽  
Cholesteryl Ester Transfer Protein ◽  
Cholesterol Efflux ◽  
Hdl Cholesterol ◽  
Lipid Transfer ◽  
Protein Activity ◽  
Cholesterol Efflux Capacity ◽  
Transfer Protein

Introduction: A potential approach to reduce CV risk is to increase HDL-C levels. This could be achieved by reducing cholesteryl ester transfer protein (CETP) activity. Dalcetrapib, which modulates CETP activity by changing its conformation and raises HDL-C without inhibiting CETP-induced pre-β-HDL formation in humans, was shown to decrease progression of atherosclerosis in rabbits. Hypothesis: Investigate the modifications of HDL particle size distribution and cholesterol efflux capacity of serum produced by dalcetrapib in normocholesterolemic rabbits. Methods: New Zealand white rabbits were treated with dalcetrapib (300 mg/kg as food admix) or placebo for 14 days. We evaluated CETP conformation and mass by ELISAs (including antibodies sensitive to conformational change), CETP activity by fluorescent lipid transfer, lipid profile and apoA-I distribution in HDL subclasses by 2D-non denaturing gradient gels (2D-NDGGE). Cholesterol efflux capacity of rabbit sera was determined after loading cells with 3 H-free cholesterol, using HepG2 hepatocytes to measure SR-BI-dependent efflux and by inducing ABCA1 or ABCG1 expression in BHK cells. Results: Dalcetrapib modified the conformation of rabbit CETP in vitro and in vivo and, after 14 days, this was associated with increased CETP mass (+50%, p<0.001) and reduced CETP activity (-86%, p<0.001). Total cholesterol was increased with dalcetrapib (+178%, p<0.001), due to a higher HDL-C level. In contrast, dalcetrapib reduced LDL-C and triglycerides by 41% (p<0.01) and 48% (p<0.001). Serum analysis by 2D-NDGGE showed that total rabbit apoA-I was increased 1.7- fold in animals treated with dalcetrapib. This was associated with an increase in large HDL but also in small α-migrating HDL with pre-β-HDL size. Cholesterol efflux assays showed that ABCA1-, ABCG1- and SR-BI-dependent efflux were all increased in dalcetrapib-treated rabbits (+24%, p=0.038; +21%, p=0.021; +44%, p<0.001). Conclusion: Modulation of CETP activity and conformation by dalcetrapib increases HDL-C and apoA-I levels and affects apoA-I distribution in HDL subclasses. These changes are associated with increased cholesterol efflux capacity, suggesting that HDL functionality is preserved in dalcetrapib-treated chow-fed rabbits.

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