Function of glutaredoxin 3 (Grx3) in oxidative stress response caused by iron homeostasis disorder inCandida albicans

2017 ◽  
Vol 12 (15) ◽  
pp. 1397-1412 ◽  
Author(s):  
Dan Zhang ◽  
Yijie Dong ◽  
Qilin Yu ◽  
Zhang Kai ◽  
Meng Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Iron Homeostasis ◽  
Oxidative Stress Response ◽  
Incandida Albicans

Proteomic analysis of the oxidative stress response inCandida albicans

PROTEOMICS ◽  
2007 ◽  
Vol 7 (5) ◽  
pp. 686-697 ◽  
Author(s):  
Harald Kusch ◽  
Susanne Engelmann ◽  
Dirk Albrecht ◽  
Joachim Morschhäuser ◽  
Michael Hecker
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Proteomic Analysis ◽  
Oxidative Stress Response ◽  
Incandida Albicans

Iron homeostasis and management of oxidative stress response in bacteria

Metallomics ◽  
2011 ◽  
Vol 3 (6) ◽  
pp. 540 ◽  
Author(s):  
Pierre Cornelis ◽  
Qing Wei ◽  
Simon C. Andrews ◽  
Tiffany Vinckx
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Iron Homeostasis ◽  
Oxidative Stress Response

scholarly journals Transcriptional Response of Leptospira interrogans to Iron Limitation and Characterization of a PerR Homolog

2010 ◽  
Vol 78 (11) ◽  
pp. 4850-4859 ◽  
Author(s):  
Miranda Lo ◽  
Gerald L. Murray ◽  
Chen Ai Khoo ◽  
David A. Haake ◽  
Richard L. Zuerner ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Iron Homeostasis ◽  
Storage Proteins ◽  
Iron Acquisition ◽  
Bacterial Species ◽  
Transcriptional Response ◽  
Oxidative Stress Response ◽  
Wild Type ◽  
In The Wild

ABSTRACT Leptospirosis is a globally significant zoonosis caused by Leptospira spp. Iron is essential for growth of most bacterial species. Since iron availability is low in the host, pathogens have evolved complex iron acquisition mechanisms to survive and establish infection. In many bacteria, expression of iron uptake and storage proteins is regulated by Fur. L. interrogans encodes four predicted Fur homologs; we have constructed a mutation in one of these, la1857. We conducted microarray analysis to identify iron-responsive genes and to study the effects of la1857 mutation on gene expression. Under iron-limiting conditions, 43 genes were upregulated and 49 genes were downregulated in the wild type. Genes encoding proteins with predicted involvement in inorganic ion transport and metabolism (including TonB-dependent proteins and outer membrane transport proteins) were overrepresented in the upregulated list, while 54% of differentially expressed genes had no known function. There were 16 upregulated genes of unknown function which are absent from the saprophyte L. biflexa and which therefore may encode virulence-associated factors. Expression of iron-responsive genes was not significantly affected by mutagenesis of la1857, indicating that LA1857 is not a global regulator of iron homeostasis. Upregulation of heme biosynthetic genes and a putative catalase in the mutant suggested that LA1857 is more similar to PerR, a regulator of the oxidative stress response. Indeed, the la1857 mutant was more resistant to peroxide stress than the wild type. Our results provide insights into the role of iron in leptospiral metabolism and regulation of the oxidative stress response, including genes likely to be important for virulence.

scholarly journals IdeR, a DtxR Family Iron Response Regulator, Controls Iron Homeostasis, Morphological Differentiation, Secondary Metabolism, and the Oxidative Stress Response inStreptomyces avermitilis

2018 ◽  
Vol 84 (22) ◽  
Author(s):  
Yaqing Cheng ◽  
Renjun Yang ◽  
Mengya Lyu ◽  
Shiwei Wang ◽  
Xingchao Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Secondary Metabolism ◽  
Iron Homeostasis ◽  
Morphological Differentiation ◽  
Oxidative Stress Response ◽  
Regulatory Role ◽  
Intracellular Iron ◽  
Content Type

ABSTRACTIron, an essential element for microorganisms, functions as a vital cofactor in a wide variety of key metabolic processes. On the other hand, excess iron may have toxic effects on bacteria by catalyzing the formation of reactive oxygen species through the Fenton reaction. The prevention of iron toxicity requires the precise control of intracellular iron levels in bacteria. Mechanisms of iron homeostasis in the genusStreptomyces(the producers of various antibiotics) are poorly understood.Streptomyces avermitilisis the industrial producer of avermectins, which are potent anthelmintic agents widely used in medicine, agriculture, and animal husbandry. We investigated the regulatory role of IdeR, a DtxR family regulator, inS. avermitilis. In the presence of iron, IdeR binds to a specific palindromic consensus sequence in promoters and regulates 14 targets involved in iron metabolism (e.g., iron acquisition, iron storage, heme metabolism, and Fe-S assembly). IdeR also directly regulates 12 targets involved in other biological processes, including morphological differentiation, secondary metabolism, carbohydrate metabolism, and the tricarboxylic acid (TCA) cycle.ideRtranscription is positively regulated by the peroxide-sensing transcriptional regulator OxyR. A newly constructedideRdeletion mutant (DideR) was found to be less responsive to iron levels and more sensitive to H2O2treatment than the wild-type strain, indicating thatideRis essential for oxidative stress responses. Our findings, taken together, demonstrate that IdeR plays a pleiotropic role in the overall coordination of metabolism inStreptomycesspp. in response to iron levels.IMPORTANCEIron is essential to almost all organisms, but in the presence of oxygen, iron is both poorly available and potentially toxic.Streptomycesspecies are predominantly present in soil where the environment is complex and fluctuating. So far, the mechanism of iron homeostasis inStreptomycesspp. remains to be elucidated. Here, we characterized the regulatory role of IdeR in the avermectin-producing organismS. avermitilis. IdeR maintains intracellular iron levels by regulating genes involved in iron absorption and storage. IdeR also directly regulates morphological differentiation, secondary metabolism, and central metabolism.ideRis under the positive control of OxyR and is indispensable for an efficient response to oxidative stress. This investigation uncovered that IdeR acts as a global regulator coordinating iron homeostasis, morphological differentiation, secondary metabolism, and oxidative stress response inStreptomycesspecies. Elucidation of the pleiotropic regulation function of IdeR provides new insights into the mechanisms of howStreptomycesspp. adapt to the complex environment.

scholarly journals Energetic Consequences of Nitrite Stress in Desulfovibrio vulgaris Hildenborough, Inferred from Global Transcriptional Analysis

2006 ◽  
Vol 72 (6) ◽  
pp. 4370-4381 ◽  
Author(s):  
Qiang He ◽  
Katherine H. Huang ◽  
Zhili He ◽  
Eric J. Alm ◽  
Matthew W. Fields ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Iron Homeostasis ◽  
Oxidative Stress Response ◽  
Nitrite Reduction ◽  
Transcriptional Analysis ◽  
Cell Physiology ◽  
Desulfovibrio Vulgaris ◽  
Genes Encoding ◽  
Stressed Cells

ABSTRACT Many of the proteins that are candidates for bioenergetic pathways involved with sulfate respiration in Desulfovibrio spp. have been studied, but complete pathways and overall cell physiology remain to be resolved for many environmentally relevant conditions. In order to understand the metabolism of these microorganisms under adverse environmental conditions for improved bioremediation efforts, Desulfovibrio vulgaris Hildenborough was used as a model organism to study stress response to nitrite, an important intermediate in the nitrogen cycle. Previous physiological studies demonstrated that growth was inhibited by nitrite and that nitrite reduction was observed to be the primary mechanism of detoxification. Global transcriptional profiling with whole-genome microarrays revealed coordinated cascades of responses to nitrite in pathways of energy metabolism, nitrogen metabolism, oxidative stress response, and iron homeostasis. In agreement with previous observations, nitrite-stressed cells showed a decrease in the expression of genes encoding sulfate reduction functions in addition to respiratory oxidative phosphorylation and ATP synthase activity. Consequently, the stressed cells had decreased expression of the genes encoding ATP-dependent amino acid transporters and proteins involved in translation. Other genes up-regulated in response to nitrite include the genes in the Fur regulon, which is suggested to be involved in iron homeostasis, and genes in the Per regulon, which is predicted to be responsible for oxidative stress response.

The moonlighting protein Tsa1p is implicated in oxidative stress response and in cell wall biogenesis inCandida albicans

2005 ◽  
Vol 57 (5) ◽  
pp. 1318-1341 ◽  
Author(s):  
Constantin Urban ◽  
Xin Xiong ◽  
Kai Sohn ◽  
Klaus Schröppel ◽  
Herwig Brunner ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Wall ◽  
Stress Response ◽  
Oxidative Stress Response ◽  
Cell Wall Biogenesis ◽  
Moonlighting Protein ◽  
Incandida Albicans
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