scholarly journals Lectin Binding Sites of the Human Pituitary Adenoma Cells as Revealed by the Ferritin and Horseradish Peroxidase-Labeled Techniques

1979 ◽  
Vol 19 (12) ◽  
pp. 1183-1195
Author(s):  
TOMOKATSU HORI ◽  
MASAO MATSUTANI ◽  
HIDEO TERAO ◽  
AKIRA TAMURA ◽  
KEIJI SANO ◽  
...  
Keyword(s):  
Pituitary Adenoma ◽  
Horseradish Peroxidase ◽  
Binding Sites ◽  
Lectin Binding ◽  
Human Pituitary ◽  
Human Pituitary Adenoma ◽  
Lectin Binding Sites

Lectin-binding sites of the human pituitary adenoma cells by means of the ferritin-labeling technique

1982 ◽  
Vol 56 (1) ◽  
pp. 67-74 ◽  
Author(s):  
T. Hori ◽  
F. Nishiyama ◽  
M. Matsutani ◽  
A. Teramoto ◽  
K. Takakura ◽  
...  
Keyword(s):  
Pituitary Adenoma ◽  
Binding Sites ◽  
Lectin Binding ◽  
Human Pituitary ◽  
Human Pituitary Adenoma ◽  
Lectin Binding Sites

Localization of concanavalin A binding sites in human pituitary adenoma cells as revealed by HRP-labelling method

1983 ◽  
Vol 62 (1-2) ◽  
pp. 59-66 ◽  
Author(s):  
T. Hori ◽  
F. Nishiyama ◽  
A. Teramoto ◽  
M. Matsutani ◽  
K. Takakura ◽  
...  
Keyword(s):  
Pituitary Adenoma ◽  
Concanavalin A ◽  
Binding Sites ◽  
Human Pituitary ◽  
Human Pituitary Adenoma ◽  
Labelling Method

scholarly journals Peroxidase and gold complexes of lectins for double labeling of surface-binding sites by electron microscopy.

1977 ◽  
Vol 25 (10) ◽  
pp. 1181-1184 ◽  
Author(s):  
J Roth ◽  
M Wagner
Keyword(s):  
Electron Microscopy ◽  
Horseradish Peroxidase ◽  
Concanavalin A ◽  
Binding Sites ◽  
Lectin Binding ◽  
Helix Pomatia ◽  
Surface Binding ◽  
Double Labeling ◽  
Membrane Bound ◽  
Lectin Binding Sites

Double labeling experiments were performed for visualization of the binding sites of Concanavalin A and anti-AHel (the lectin from Helix pomatia). The anti-AHel was labeled with the colloidal gold whereas the membrane bound Concanavalin A was demonstrated by an affinity technique using horseradish peroxidase. The two markers used could be clearly distinguished electron microscopically. The specificity of the cell surface double labeling was demonstrated in the control experiments. A topological distinct localization of the both lectin-binding sites is evident.

Differences in Glycoconjugates of Adrenocorticotropic Hormone-secretory Granules between Nonadenomatous Pituitary Cells and Adenoma Cells as Detected by Double Labeling

Neurosurgery ◽  
1988 ◽  
Vol 23 (1) ◽  
pp. 52-57 ◽  
Author(s):  
Tomokatsu Hori ◽  
Fumiaki Nishiyama ◽  
Yuichi Anno ◽  
Satoshi Tanaka ◽  
Takashi Watanabe ◽  
...  
Keyword(s):  
Concanavalin A ◽  
Binding Sites ◽  
Pituitary Adenomas ◽  
Adrenocorticotropic Hormone ◽  
Secretory Granules ◽  
Lectin Binding ◽  
Pituitary Cells ◽  
Human Pituitary ◽  
Pituitary Tissue ◽  
Lectin Binding Sites

ABSTRACT Lectin binding sites of adrenocorticotropic hormone (ACTH) secretory granules of human pituitary adenomas and of nonadenomatous pituitary tissue adjacent to adenomas were studied by postembedding immunocytochemical doublestaining on ultrathin sections followed by electron microscopy. The specific hormones produced by the secretory granules were identified by labeling one side of the section with anti-human pituitary hormone antibodies conjugated to gold particles. Simultaneously, the other side was labeled with horseradish peroxidase-lectin to reveal lectin binding sites. Specimens were obtained from four human ACTH-producing pituitary adenomas and from nonadenomatous pituitary tissue surrounding three other adenomas. The four ACTH-producing adenomas showed either weak or negative reactions with concanavalin A, whereas the nonadenomatous ACTH-producing pituitary cells reacted strongly with concanavalin A. Moreover, ACTH secretory granules were significantly larger in the nonadenomatous cells than in adenoma cells. Differences in biochemical structure and ultrastructure between nonadenomatous (normal) pituitary cells and adenoma cells secreting the same specific hormones were demonstrated, and the clinical implications of the results were discussed.

Lectin-binding sites in chick limb buds

1989 ◽  
Vol 27 ◽  
pp. 82
Author(s):  
M. Narita ◽  
K. Yamashita ◽  
M. Yasuda
Keyword(s):  
Binding Sites ◽  
Lectin Binding ◽  
Limb Buds ◽  
Lectin Binding Sites

Ultrastructural demonstration of lectin binding sites in the Golgi apparatus of rat epiphyseal chondrocytes

1988 ◽  
Vol 89 (2) ◽  
pp. 177-184 ◽  
Author(s):  
A. Velasco ◽  
J. Hidalgo ◽  
M. M�ller ◽  
G. Garcia-Herdugo
Keyword(s):  
Golgi Apparatus ◽  
Binding Sites ◽  
Lectin Binding ◽  
Lectin Binding Sites

The EFEMP1 Gene: A Frequent Target for Epigenetic Silencing in Multiple Human Pituitary Adenoma Subtypes

2013 ◽  
Vol 98 (3) ◽  
pp. 200-211 ◽  
Author(s):  
Cuong V. Duong ◽  
Kiren Yacqub-Usman ◽  
Richard D. Emes ◽  
Richard N. Clayton ◽  
William E. Farrell
Keyword(s):  
Pituitary Adenoma ◽  
Epigenetic Silencing ◽  
Human Pituitary ◽  
Human Pituitary Adenoma

Lectin binding sites of the mouse ovary, intraovarian and ovulated ova

1984 ◽  
Vol 80 (6) ◽  
pp. 527-533 ◽  
Author(s):  
T. -C. Wu ◽  
M. -C. Lee ◽  
Y. -J. Wan ◽  
I. Damjanov
Keyword(s):  
Binding Sites ◽  
Lectin Binding ◽  
Mouse Ovary ◽  
Lectin Binding Sites
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