Lectin-binding sites of the human pituitary adenoma cells by means of the ferritin-labeling technique

1982 ◽  
Vol 56 (1) ◽  
pp. 67-74 ◽  
Author(s):  
T. Hori ◽  
F. Nishiyama ◽  
M. Matsutani ◽  
A. Teramoto ◽  
K. Takakura ◽  
...  
1979 ◽  
Vol 19 (12) ◽  
pp. 1183-1195
Author(s):  
TOMOKATSU HORI ◽  
MASAO MATSUTANI ◽  
HIDEO TERAO ◽  
AKIRA TAMURA ◽  
KEIJI SANO ◽  
...  

1983 ◽  
Vol 62 (1-2) ◽  
pp. 59-66 ◽  
Author(s):  
T. Hori ◽  
F. Nishiyama ◽  
A. Teramoto ◽  
M. Matsutani ◽  
K. Takakura ◽  
...  

Neurosurgery ◽  
1988 ◽  
Vol 23 (1) ◽  
pp. 52-57 ◽  
Author(s):  
Tomokatsu Hori ◽  
Fumiaki Nishiyama ◽  
Yuichi Anno ◽  
Satoshi Tanaka ◽  
Takashi Watanabe ◽  
...  

ABSTRACT Lectin binding sites of adrenocorticotropic hormone (ACTH) secretory granules of human pituitary adenomas and of nonadenomatous pituitary tissue adjacent to adenomas were studied by postembedding immunocytochemical doublestaining on ultrathin sections followed by electron microscopy. The specific hormones produced by the secretory granules were identified by labeling one side of the section with anti-human pituitary hormone antibodies conjugated to gold particles. Simultaneously, the other side was labeled with horseradish peroxidase-lectin to reveal lectin binding sites. Specimens were obtained from four human ACTH-producing pituitary adenomas and from nonadenomatous pituitary tissue surrounding three other adenomas. The four ACTH-producing adenomas showed either weak or negative reactions with concanavalin A, whereas the nonadenomatous ACTH-producing pituitary cells reacted strongly with concanavalin A. Moreover, ACTH secretory granules were significantly larger in the nonadenomatous cells than in adenoma cells. Differences in biochemical structure and ultrastructure between nonadenomatous (normal) pituitary cells and adenoma cells secreting the same specific hormones were demonstrated, and the clinical implications of the results were discussed.


1989 ◽  
Vol 27 ◽  
pp. 82
Author(s):  
M. Narita ◽  
K. Yamashita ◽  
M. Yasuda

1988 ◽  
Vol 89 (2) ◽  
pp. 177-184 ◽  
Author(s):  
A. Velasco ◽  
J. Hidalgo ◽  
M. M�ller ◽  
G. Garcia-Herdugo

2013 ◽  
Vol 98 (3) ◽  
pp. 200-211 ◽  
Author(s):  
Cuong V. Duong ◽  
Kiren Yacqub-Usman ◽  
Richard D. Emes ◽  
Richard N. Clayton ◽  
William E. Farrell

1984 ◽  
Vol 80 (6) ◽  
pp. 527-533 ◽  
Author(s):  
T. -C. Wu ◽  
M. -C. Lee ◽  
Y. -J. Wan ◽  
I. Damjanov

1984 ◽  
Vol 7 (4) ◽  
pp. 307-311 ◽  
Author(s):  
Robert Oosterom ◽  
G. Blaauw ◽  
R. Singh ◽  
T. Verleun ◽  
S. W. J. Lamberts

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