scholarly journals Comprehensive phenotypic and statistical based analysis of foxtail millet (Setaria italic L.) lines for parental line selection

2017 ◽  
Vol 6 (11) ◽  
pp. 1736 ◽  
Author(s):  
Chandra Sekhar Akila ◽  
Palakurthi Ramesh ◽  
Gurulakshmi Kola ◽  
Mallikarjuna Gunti ◽  
Puli Chandra Obul Reddy ◽  
...  
Keyword(s):  
Phenotypic Variability ◽  
Foxtail Millet ◽  
Principal Component ◽  
Breeding Systems ◽  
Phenotypic Traits ◽  
Parental Line ◽  
Panicle Length ◽  
Pure Lines ◽  
Total Seed ◽  
Panicle Weight

Minor millets are the small-grained cereals that belong to family Poaceae, with local importance as a food source but are often called as underutilized plant species.   Foxtail millet is one among  the oldest cultivated small diploid, C4 Panicoid, with short life cycle, and inbreeding nature. The genetic diversity of the foxtail millet is important for breeding systems, geographical distribution studies, assist in conservation of genetic resources for high yielding varietal development in breeding programs, expansion of the genetic base and for identification of genes for various phenotypic traits. Keeping in view of this an attempt has made to develop pure lines from local farmers preserved populations and phenotypically characterize them along with four released cultivars.  A total of eleven farmers varieties and four released cultivars were evaluated for seven quantitative traits in complete random block desing at net house conditions.  The maximum coefficient of variation was observed for panicle exertion, followed by number of tillers/plant and panicle weight. High positive significant correlations was observed for Plant height, panicle length, panicle weight and total seed weight with other traits under study. Panicle exertion exhibit negative correlations with panicle length, panicle weight and no of tillers. The results of principal component analysis (PCA) explains variability of 95.43% in the fifteen genotypes for the traits under study that corresponds to first 4 eigen values with greater than 0.60.  The proportions of the total variance explained by the first 4 principal components (PCs) were 42.98%, 71.02%, 85.43% and 95.43% respectively. Wards method based genetic similarity or dissimilarity clustering based on seven morphological traits among the fifteen foxtail millet genotypes was able to separate and grouped them into 2 major clusters I and II.  The genetic and phenotypic variability present in the foxtail millet genotypes give opportunity for plant breeders for effective selection of specific donor lines for foxtail millet improvement.

scholarly journals Defining a Phenotypic Variability and Productivity in Wild Type Red Clover Germplasm

2020 ◽  
Vol 12 (9) ◽  
pp. 52
Author(s):  
Giedrius Petrauskas ◽  
Vaclovas Stukonis ◽  
Eglė Norkevičienė
Keyword(s):  
Biomass Yield ◽  
Phenotypic Variability ◽  
Red Clover ◽  
Principal Component ◽  
Ex Situ ◽  
Phenotypic Traits ◽  
Wild Type ◽  
Plant Structure ◽  
Crude Fibre ◽  
Important Trait

Abiotic and biotic factors can cause great damage to crops. So, a key approach is to investigate whether the crops’ wild relatives are more flexible to withstand abiotic and biotic stress. As well as to evaluate their phenotypic variability and productivity in response to changing climatic conditions. In this study, red clover germplasm was collected from natural red clover habitats and a field trial was arranged ex situ. Twelve phenotypic traits and their effects on final harvest were analysed in 2018–2019. Principal component analysis (PCA) demonstrated that the most important trait for biomass yield was the height of the plant during the first season of harvest (2018). Interestingly, that the most significant trait in the second year of harvest (2019) was growth habit. Meantime, two way-joining analysis was performed to extent of phenotypic variation within and among red clover accessions, based on the most important trait for biomass yield. We found three main groups based on variation in plant height: “cultivars”, “wilds” and “mediators”. This analysis leads to identify typical populations of wild type red clover, which has not been done yet. Finally, the feed value of each red clover accession was analysed. It was found that “cultivars” have a higher level of crude proteins, while “wilds” contains higher levels of crude fibre. This indicates that there is a relationship between plant structure elements and forage value which is particularly important to select a breeding material.

scholarly journals Genetic variability of panicle architecture in traditional finger millets from Koraput valley of Eastern Ghats for crop improvement

2021 ◽  
Author(s):  
Debabrata Panda ◽  
Amrita Tanty ◽  
Prafulla K Behera ◽  
Aloukika Panda ◽  
Kartik Lenka
Keyword(s):  
Grain Yield ◽  
Leaf Area ◽  
Finger Millet ◽  
Crop Improvement ◽  
Principal Component ◽  
Eastern Ghats ◽  
Panicle Length ◽  
Improvement Program ◽  
Panicle Number ◽  
Panicle Weight

Abstract Panicle phenotyping is most demanding targets in crop breeding programs as panicle is an important plant parts that influences the grain yield. Diversity of panicle traits were analyzed in 20 traditional finger millet genotypes and three hybrid varieties from Koraput valley of Eastern Ghats. Significant difference of panicle characteristics like panicle length, panicle number, panicle weight, flag leaf area, panicle angle and grain yield were observed in experimented finger millet genotypes. In regards to principal component analysis, the first two axis of principal component obtained 52.97% of total variation and reflected huge difference between explored genotypes. Highest positive loading was observed for leaf weight followed by panicle number, panicle weight and panicle length and leaf area and are the major determinant for phenotypic variability. All the studied traits showed maximized phenotypic coefficient of variation (PCV) over genotypic variation (GCV) and lower differences found among them. The genotypes showed maximum broad-sense of heritability value for grain yield (82.27%) and panicle weight (75.96%) compared to other traits. In addition, genetic advance as mean% (GAM) ranged from 11.01–29.26% and high GAM was recorded for panicle angle, leaf area, panicle weight, panicle number and yield. It revealed that these characters can be used as selection criteria in crop improvement program for improving grain yield. Traditional finger millet genotypes such as Bhadi, Murda, Telgu and Chilli recorded superior panicle traits and Limca and Kalia showed better grain yield at par with the hybrids, which can be utilized in future crop improvement program.

scholarly journals Evaluation of indigenous aromatic rice cultivars from sub-Himalayan Terai region of India for nutritional attributes and blast resistance

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Debayan Mondal ◽  
Prudveesh Kantamraju ◽  
Susmita Jha ◽  
Gadge Sushant Sundarrao ◽  
Arpan Bhowmik ◽  
...  
Keyword(s):  
Nutritive Value ◽  
Amylose Content ◽  
Blast Resistance ◽  
Starch Content ◽  
Low Cost ◽  
Principal Component ◽  
Blast Disease ◽  
Basmati Rice ◽  
Phenotypic Traits ◽  
Rice Cultivars

AbstractIndigenous folk rice cultivars often possess remarkable but unrevealed potential in terms of nutritional attributes and biotic stress tolerance. The unique cooking qualities and blissful aroma of many of these landraces make it an attractive low-cost alternative to high priced Basmati rice. Sub-Himalayan Terai region is bestowed with great agrobiodiversity in traditional heirloom rice cultivars. In the present study, ninety-nine folk rice cultivars from these regions were collected, purified and characterized for morphological and yield traits. Based on traditional importance and presence of aroma, thirty-five genotypes were selected and analyzed for genetic diversity using micro-satellite marker system. The genotypes were found to be genetically distinct and of high nutritive value. The resistant starch content, amylose content, glycemic index and antioxidant potential of these genotypes represented wide variability and ‘Kataribhog’, ‘Sadanunia’, ‘Chakhao’ etc. were identified as promising genotypes in terms of different nutritional attributes. These cultivars were screened further for resistance against blast disease in field trials and cultivars like ‘Sadanunia’, ‘T4M-3-5’, ‘Chakhao Sampark’ were found to be highly resistant to the blast disease whereas ‘Kalonunia’, ‘Gobindabhog’, ‘Konkanijoha’ were found to be highly susceptible. Principal Component analysis divided the genotypes in distinct groups for nutritional potential and blast tolerance. The resistant and susceptible genotypes were screened for the presence of the blast resistant pi genes and association analysis was performed with disease tolerance. Finally, a logistic model based on phenotypic traits for prediction of the blast susceptibility of the genotypes is proposed with more than 80% accuracy.

scholarly journals Codon usage bias and environmental adaptation in microbial organisms

2021 ◽  
Author(s):  
Davide Arella ◽  
Maddalena Dilucca ◽  
Andrea Giansanti
Keyword(s):  
Codon Usage ◽  
Codon Usage Bias ◽  
Pathogenic Bacteria ◽  
Large Scale ◽  
Synonymous Codon ◽  
Principal Component ◽  
Gene Copy ◽  
Phenotypic Traits ◽  
Translational Efficiency ◽  
Microbial Organisms

AbstractIn each genome, synonymous codons are used with different frequencies; this general phenomenon is known as codon usage bias. It has been previously recognised that codon usage bias could affect the cellular fitness and might be associated with the ecology of microbial organisms. In this exploratory study, we investigated the relationship between codon usage bias, lifestyles (thermophiles vs. mesophiles; pathogenic vs. non-pathogenic; halophilic vs. non-halophilic; aerobic vs. anaerobic and facultative) and habitats (aquatic, terrestrial, host-associated, specialised, multiple) of 615 microbial organisms (544 bacteria and 71 archaea). Principal component analysis revealed that species with given phenotypic traits and living in similar environmental conditions have similar codon preferences, as represented by the relative synonymous codon usage (RSCU) index, and similar spectra of tRNA availability, as gauged by the tRNA gene copy number (tGCN). Moreover, by measuring the average tRNA adaptation index (tAI) for each genome, an index that can be associated with translational efficiency, we observed that organisms able to live in multiple habitats, including facultative organisms, mesophiles and pathogenic bacteria, are characterised by a reduced translational efficiency, consistently with their need to adapt to different environments. Our results show that synonymous codon choices might be under strong translational selection, which modulates the choice of the codons to differently match tRNA availability, depending on the organism’s lifestyle needs. To our knowledge, this is the first large-scale study that examines the role of codon bias and translational efficiency in the adaptation of microbial organisms to the environment in which they live.

scholarly journals Genetic diversity analysis of parental lines for hybrid development in rice (Oryza sativa L.)

2013 ◽  
Vol 37 (4) ◽  
pp. 617-624 ◽  
Author(s):  
MJ Hasan ◽  
Umma Kulsum ◽  
MMH Rahman ◽  
MMH Chowdhury ◽  
AZMKA Chowdhury
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa L ◽  
Principal Component ◽  
Mean Value ◽  
Cluster Distance ◽  
Parental Lines ◽  
Almost All ◽  
Yield Maximization ◽  
Hybrid Development ◽  
Panicle Weight

Genetic divergence of 40 parental lines comprising 30 restorer and 10 maintainer lines were studied through Mohalanobis's D2 and principal component analysis for eleven characters. Genotypes were grouped into five different clusters. Cluster V comprised maximum number of genotypes (thirteen) followed by cluster I and II. The inter-cluster distance was maximum between clusters I and V (13.495) indicating wide genetic diversity between these two clusters followed by the distance between cluster I and 11 (9.489), cluster IV, and cluster V (8.969) and cluster I and cluster III (8.039). The minimum inter-cluster distance was observed between cluster II and cluster III (3.034) followed by cluster 111 and cluster IV (3.834) and cluster II and cluster V (4.945) indicating that the genotypes of these clusters were genetically close. The intra cluster distance in the entire five clusters was more or less low which indicated that the genotypes within the same cluster were closely related. Among the characters panicle weight contributed most for divergence in the studied parental lines. Difference in cluster means existed for almost all the characters studied. Highest mean value for number of effective tillers (7.8), days to 50% flowering (95.5), panicles/m2 (192.6), panicle weight (2.9), spikelet fertility (84.8), number of grains/panicle (177.8), days to maturity (123.6), and grain yield/plot (1065.5) were observed in cluster I indicated the parental lines fallen in this cluster having the genetic potentiality to contribute better for yield maximization of hybrid rice. DOI: http://dx.doi.org/10.3329/bjar.v37i4.14386 Bangladesh J. Agril. Res. 37(4): 617-624, December 2012

scholarly journals Rhamnella intermedia (Rhamnaceae), a new evergreen species from southwest Guangxi

PhytoKeys ◽  
2020 ◽  
Vol 159 ◽  
pp. 115-126
Author(s):  
Zhiqiang Lu ◽  
Yongshuai Sun
Keyword(s):  
New Species ◽  
Related Species ◽  
Principal Component ◽  
Phenotypic Traits ◽  
Evergreen Species ◽  
Closely Related Species ◽  
Field Investigations ◽  
Sequence Variations ◽  
Dried Fruit ◽  
Nuclear Its

Rhamnella intermedia, a new evergreen species from southwest Guangxi, is described and illustrated in this study. This species is similar to R. brachycarpa by the size and ratio of length to width of dried fruit and seeds, by which it differs from R. rubrinervis and R. tonkinensis. However, it differs from R. brachycarpa by rarely mucronate seed apices, larger ratio of length to width of leaves, leaf apices acuminate to long acuminate, shorter leaf petioles, and longer fruiting pedicels. Principal component analysis based on phenotypic traits further recognised three separated groups. Rhamnella rubrinervis and R. tonkinensis were clustered into one group; the other two groups represented R. brachycarpa and two Guangxi populations, respectively. Furthermore, phylogenetic analysis of nuclear ITS sequence variations highly supported that the two Guangxi populations represented an independent evolutionary lineage and were closest to R. rubrinervis. Four fixed nucleotide sites were found and were different from R. rubrinervis. However, besides the differentiated traits in seeds and fruit, densely pilose young branches also separated them from R. rubrinervis. In addition, during our field investigations, none of the three closely related species were found at locations where this new species was distributed. Therefore, this new species, based on the two Guangxi populations, is named R. intermedia. The key to four closely related species is also presented.

scholarly journals Genetic diversity of common bean (Phaseolus vulgaris L.) breeding collection in Serbia

Genetika ◽  
2019 ◽  
Vol 51 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Aleksandra Savic ◽  
Milka Brdar-Jokanovic ◽  
Miodrag Dimitrijevic ◽  
Sofija Petrovic ◽  
Milan Zdravkovic ◽  
...  
Keyword(s):  
Common Bean ◽  
Gene Pool ◽  
Gene Diversity ◽  
Principal Component ◽  
Molecular Data ◽  
Phenotypic Traits ◽  
Gene Pools ◽  
Vegetable Crops ◽  
Novi Sad

The characterization of 41 common bean cultivars and landraces from breeding collection of Institute of Field and Vegetable Crops, Novi Sad, Serbia, was done based on phenotypic traits and microsatellite markers. Phenotypic traits were chosen from Bioversity International descriptor list. In addition, main yield components were investigated. Analysis of phaseolin type revealed affiliation of cultivars and landraces to Mesoamerican or Andean gene pool. Cultivars and landraces demonstrated significant diversity level with regard to studied phenotypic traits. Identified variation showed high potential for developing new cultivars with desirable combination of traits. Principal component analysis based on phenotypic traits separated bean cultivars and landraces in two groups, which corresponded to Mesoamerican and Andean determined according to phaseolin type. Putative hybrids, with combination of traits between gene pools were also identified. Analysis of microsatellite data, using twenty-two SSR primer pairs, showed medium gene diversity in studied material. Microsatellite-based cluster analysis separated genotypes in two discrete clusters and several subclusters. No clear separation according to gene pool was found between the clusters, however grouping according to gene pool and patterns of phenotypic variation, following these gene pools, were observed within subclusters. Knowledge on detailed relationships of cultivars and landraces based on phenotypic and molecular data would facilitate identification of candidates for future breeding.

scholarly journals Variation of Phenotypic Traits in Twelve Bambara Groundnut (Vigna subterranea (L.) Verdc.) Genotypes and Two F2 Bi-Parental Segregating Populations

Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1451
Author(s):  
Xiuqing Gao ◽  
Aliyu Siise Abdullah Bamba ◽  
Aloyce Callist Kundy ◽  
Kumbirai Ivyne Mateva ◽  
Hui Hui Chai ◽  
...  
Keyword(s):  
Southern Africa ◽  
Negative Relationship ◽  
Principal Component ◽  
Bambara Groundnut ◽  
Structured Populations ◽  
Phenotypic Trait ◽  
Phenotypic Traits ◽  
Potential Contribution ◽  
Vigna Subterranea ◽  
East West

Underutilised species such as bambara groundnut (Vigna subterranea (L.) Verdc.) have the potential to contribute significantly to meeting food and nutritional needs worldwide. We evaluated phenotypic traits in twelve bambara groundnut genotypes from East, West and Southern Africa and Southeast Asia and two F2 bi-parental segregating populations derived from IITA-686 ×Tiga Nicuru and S19-3 ×DodR to determine phenotypic trait variation and their potential contribution to the development of improved crop varieties. All phenotypic traits in twelve genotypes were significantly influenced (p < 0.01) by genotypes. Principal component analysis (PCA) showed that PC1 accounted for 97.33% variation and was associated with four genotypes collected from East and Southern Africa. PC2 accounted for 2.48% of the variation and was associated with five genotypes collected from East, West and Southern Africa. Transgressive segregation for a number of traits was observed in the two F2 bi-parental populations, as some individual lines in the segregating populations showed trait values greater or less than their parents. The variability between twelve genotypes and the two F2 bi-parental segregating populations and the negative relationship between plant architectural traits and yield related traits provide resources for development of structured populations and breeding lines for bambara groundnut breeding programme.

scholarly journals Profils morpho-biometriques de la poule locale du Burkina Faso

2020 ◽  
Vol 14 (6) ◽  
pp. 2240-2256
Author(s):  
S. Pinde ◽  
A.S.R. Tapsoba ◽  
M. Sanou ◽  
F.G. Traore ◽  
R.W. Ouedraogo ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Phenotypic Variability ◽  
Principal Component ◽  
Phenotypic Characterization ◽  
Accurate Identification ◽  
Ecological Zones ◽  
Large Size ◽  
Dimorphisme Sexuel ◽  
Rational Management ◽  
Agro Ecological Zones

Au Burkina Faso les informations sur la poule locale sont rares entrainant des difficultés objectives de son exploitation rationnelle. Un total de 948 individus (692 femelles et 256 mâles) provenant de 251 élevages reparties dans les 3 zones agro-écologiques du Burkina Faso a été évalué pour 13 traits corporels quantitatifs et 09 qualitatifs dans le but de caractériser la variabilité phénotypique de la poule locale. Il ressort 13 coloris de plumage avec le blanc (19,16%) et l’herminé (16,75%) comme couleurs dominantes. Le site et le sexe ont eu une influence (p<0,05) sur les traits corporels analysés. L’Analyse en Composante Principale révèle l’existence de 2 sous-populations: une souche légère de petit gabarit (0,91 kg) dans les trois zones agro-écologiques et une souche lourde de grand gabarit (1,37 kg) dans les zones soudano-sahélienne et soudanienne, laissant présager des différences d’adaptation liées aux facteurs environnementaux. Un dimorphisme sexuel en faveur des mâles a été observé au niveau du poids vif moyen (1,64 kg vs 1,14 kg), de la longueur du corps (42,16 cm vs 37,30 cm) et du périmètre thoracique (29,01 cm vs 25,96 cm). Les résultats rapportés dans cette étude serviront de base pour l’élaboration de programmes de développement basés sur une exploitation rationnelle de la poule locale.Mots clés : Caractérisation phénotypique, poules locales, zones agro-écologiques, Burkina Faso.   In Burkina Faso, information on poultry genetic resources is scarce and this scenario does not allow an accurate identification and management strategy for this species. A total of 948 individuals (692 females and 256 males) belonging to 251 different household, accros the 3 agro-ecological zones of Burkina Faso have been assessed for 13 quantitative traits and 9 qualitative traits in order to characterize phenotypic variability of local poultry. The most frequent plumage colors are white (19.16%) and hermine (16.75%) out of 13 different coulors identified. The site and the sex of the individuals had a statistically significant influence (P <0.05) on the 13 body measurements analyzed. Principal Component Analysis reveals the existence of 2 subpopulations in the 3 agro-ecological zones, a light hen with small size (0.91 kg) and a heavy and large size hen (1.37 kg), almost encountered in Sudan-Sahel and Sudan areas, suggesting differences in adaptation due to environmental factors. A sexual dimorphism was observed in favor of the males considering bodyweight (1,64 kg vs 1,14 kg), body length (42.16 cm vs 37.30 cm) and the Heart girth (29.01 cm vs 25.96 cm for females). The results reported in the current study will be useful for the establishment of breeding programme based on rational management of local poultry in Burkina Faso.Keywords : Phenotypic characterization, local hens, agro-ecological zones, Burkina Faso.  

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